苏淮猪抗腹泻MUC13基因rs319699771位点多态性 及其与经济性状的关联

doi:10.3864/j.issn.0578-1752.2019.08.014

Abstract

Abstract:

【Background】 MUC13 gene is one of the main genes, which can regulate the infection of enterotoxigenic Escherichia coli F4ac, and cause diarrhea in piglets before weaning. The rs319699771 locus of the gene (G/A mutation) can accurately identify susceptible and resistant individuals, among which GG type is an anti-diarrhea genotype. The anti-diarrhea molecular breeding of Suhuai pig has been implemented by selecting GG genotype individuals, but whether the selection of GG genotype individuals will have adverse effects on other important economic traits, such as growth, carcass and meat quality, which is not yet clear. 【Objective】 The purpose of this study was to analyze the association between the locus and other economic traits to determine whether the molecular selection based on the rs319699771 locus of anti-diarrhea MUC13 gene would have adverse effects on other economic traits of Suhuai pig. 【Method】 In this experiment, 313 Suhuai fattening pigs weighting 87.61±0.54 kg were tested as experimental animals, and their carcass and meat quality traits were determined through slaughter. 261 Suhuai gilt pigs aging for 161.1±0.5 d were tested as experimental animals, and their growth and body size phenotype were also determined. At the same time, the corresponding ear tissue samples of Suhuai fattening pigs and gilt pigs were collected to extract tissue DNA. After Multiplex-PCR reactions, the polymorphism of the rs319699771 locus of MUC13 gene was detected in each pig. The association analysis between the polymorphism genotype of the rs319699771 locus of MUC13 gene and meat quality, carcass and growth traits were conducted by using the general linear model in SAS software. 【Result】 The results of polymorphism of rs319699771 locus of MUC13 gene showed that among the frequency of anti-diarrhea G allele of rs319699771 locus of MUC13 gene, the male and female pigs of the fattening pigs reached 0.695 and 0.634, respectively, and the anti-diarrhea GG genotype frequency was 0.467 and 0.373, respectively, in the male and female fattening pigs. While the locus G allele among the male and female pigs of the gilt reached 0.690 and 0.705, respectively, and the anti-diarrhea GG genotype frequency was 0.508 and 0.480, respectively, in the male and female gilt pigs. This indicated that the frequency of anti-diarrhea gene in Suhuai pig belonged to a higher level, and it could be feasible for the further improvement of anti-diarrhea GG frequency in the Suhuai pig through molecular breeding. The association analysis between polymorphism of rs319699771 locus of MUC13 gene and economic traits of Suhuai pig showed that there was no significant association between the locus’s polymorphism and carcass, meat quality traits in the fattening pigs (P>0.05), and it could be seen that increasing the breeding of the rs319699771 locus of MUC13 gene in Suhuai fattening pigs would not affect the carcass and meat quality traits. The locus’s polymorphism in the gilt extremely associated with the ham circumference index (P<0.01), and on average, the ham circumference of GG type individuals at this locus was about 1.46 cm longer than that of AG type individuals, and about 3 cm longer than that of AA type individuals. Also, the locus tended to associate with daily gain and terminal measurement weight (P<0.10), GG type individuals showed an upward trend compared with AA and AG type individuals. All of the three traits, GG genotype was a favorable genotype. The results showed that the breeding of the anti-diarrhea GG genotype of this locus could improve the growth traits of Suhuai gilt pigs. 【Conclusion】 Based on these results, the anti-diarrhea allele frequency of the rs319699771 locus of MUC13 gene in Suhuai pig was high, and the anti-diarrhea breeding was feasible, and the anti-diarrhea ability of Suhuai pig could be improved by selecting and remaining resistant homozygous GG genotype of rs319699771 locus of MUC13 gene and raising the frequency of the resistant homozygous genotype. At the same time, it could also realize the improvements of ham circumference and daily gain of Suhuai pig.

Key words: Suhuai pig, MUC13 gene, anti-diarrhea, economic traits, association analysis

LIU ChenXi,WANG BinBin,PU Guang,ZHANG Qian,CAO Yang,WANG Huan,GAO Chen,NIU PeiPei,LI PingHua,HUANG RuiHua. Polymorphism of Rs319699771 Locus of Anti-Diarrhea MUC13 Gene in Suhuai Pig Population and Their Association with Economic Traits[J].Scientia Agricultura Sinica, 2019, 52(8): 1449-1457.

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MUC13基因 MUC13 gene	引物序列 Primer sequence
PCR引物序列 PCR primer sequence	上游 Upstream	TCCTCACACCTCCTGCCTTTTC
PCR引物序列 PCR primer sequence	下游 Downstream	TCAAAACAGAGCGATTTCTGGGTAA
多重PCR连接引物序列 Multiplex-PCR binding primer sequence	MUC13_A_GFP	TGAATTTTGGCTATAGRCCTGTGGTTTTTTTTTTTTTTTTTTTTT
	MUC13_A_GFG	TTCCGCGTTCGGACTGATATTCCATGTACATTTCAGAGTCTGAGGGTTG
	MUC13_A_GFA	TACGGTTATTCGGGCTCCTGTTCCATGTACATTTCAGAGTCTGAGGGCTA

群体 Group	性别 Gender (n)	基因型频率Genotype frequency			基因频率Gene frequency
群体 Group	性别 Gender (n)	AA (n)	AG (n)	GG (n)	A	G
育肥群 Fattening pigs	母猪Sow (67)	0.104 (7)	0.522 (35)	0.373 (25)	0.366	0.634
	阉公猪Barrow (246)	0.077 (19)	0.455 (112)	0.467 (115)	0.305	0.695
	合计Total (313)	0.083 (26)	0.470 (147)	0.447 (140)	0.318	0.682
后备群 Gilt	母猪Sow (198)	0.071 (14)	0.449 (89)	0.480 (95)	0.295	0.705
	公猪Boar (63)	0.127 (8)	0.365 (23)	0.508 (32)	0.310	0.690
	合计Total (261)	0.084 (22)	0.429 (112)	0.487 (127)	0.299	0.701

基因型 Genotype	个体数 Number	胴体性状Carcass performances
基因型 Genotype	个体数 Number	胴体重 Weight of carcass (kg)	肋骨数 Number of ribs	胴体直长 Straight length of carcass (cm)	胴体斜长 Oblique length of carcass (cm)	6-7肋膘厚 Backfat thickness at 6-7 ribs (mm)	肩部最厚处膘厚 Backfat thickness at the thickest part of the shoulder (mm)	最后肋骨膘厚 Backfat thickness at the last rib (mm)	腰荐结合膘厚 Backfat thickness at the lumbosacral junction (mm)	三点平均背膘厚 Average backfat thickness (mm)
AA	26	55.89±1.45	30.33±0.75	88.14±0.75	73.97±0.69	29.17±1.26	34.11±1.28	20.06±1.08	22.48±1.40	25.56±1.11
AG	147	57.51±0.70	29.66±0.36	88.69±0.36	74.00±0.33	28.46±0.61	34.15±0.62	21.12±0.52	23.74±0.67	26.37±0.54
GG	140	57.35±0.71	29.82±0.37	88.60±0.37	73.96±0.34	28.87±0.61	34.45±0.63	21.93±0.53	23.59±0.68	26.67±0.54
P-value		0.5470	0.6778	0.7754	0.9955	0.7797	0.9064	0.1684	0.6795	0.6106

基因型 Genotype	个体数 Number	肉质性状Meat quality performances
基因型 Genotype	个体数 Number	2h亮度值 L_2h lightness	2h红度值 a_2h redness	2h黄度值 b_2h yellowness	24h亮度值 L_24h lightness	24h红度值 a_24h redness	24h黄度值 b_24h yellowness	pH_45min	pH_24h	滴水损失 Drip loss (%)	系水力 Water holding capacity (%)	肌内脂肪含量 IMF (%)
AA	26	39.71±0.69	5.10±0.30	11.50±1.67	44.31±0.77	5.76±0.30	12.36±0.26	6.43±0.06	5.65±0.04	2.41±0.24	24.83±1.09	2.07±0.15
AG	147	41.18±0.33	4.66±0.15	11.51±0.81	46.00±0.37	5.51±0.14	12.35±0.13	6.37±0.03	5.67±0.02	2.27±0.12	25.47±0.53	1.98±0.07
GG	140	41.16±0.34	4.44±0.15	12.46±0.81	46.03±0.37	5.48±0.14	12.51±0.13	6.43±0.03	5.70±0.02	2.54±0.12	24.84±0.53	1.99±0.07
P-value		0.1034	0.0843	0.5849	0.0800	0.6711	0.5332	0.2726	0.4739	0.1345	0.5645	0.8144

基因型 Genotype	个体数 Number	生长性状Growth performances
基因型 Genotype	个体数 Number	日增重 Daily gain (g·d^-1)	结测体重 Terminal measurement weight (kg)	体长 Body length (cm)	体高 Body height (cm)	胸围 Chest circumference (cm)	腹围 Abdominal circumference (cm)	管围 Cannon bone circumference (cm)	腿臀围 Ham circumference (cm)	活体背膘 Live backfat (mm)	活体眼肌面积 Live loin eye area (cm²)
AA	22	347.87±9.09	56.50±1.47	97.65±1.29	53.06±0.97	91.31±1.12	102.54±1.91	15.13±0.24	72.78±0.99^b	11.79±0.66	17.18±4.76
AG	112	362.89±4.33	58.43±0.07	98.30±0.61	53.53±0.46	92.05±0.54	103.83±0.91	15.31±0.11	74.32±0.47^ab	11.78±0.31	20.84±2.27
GG	127	370.48±4.12	59.62±0.67	99.20±0.58	54.44±0.44	91.14±0.52	102.33±0.87	15.22±0.11	75.78±0.45^a	11.73±0.03	22.03±2.16
P-value		0.0514	0.0601	0.1784	0.1942	0.4347	0.4209	0.7086	0.0045	0.9917	0.6279

Polymorphism of Rs319699771 Locus of Anti-Diarrhea MUC13 Gene in Suhuai Pig Population and Their Association with Economic Traits

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