Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (4): 625-639.doi: 10.3864/j.issn.0578-1752.2017.04.003


Drought Resistance of Sesame Germplasm Resources and Association Analysis at Adult Stage

LIU WenPing1, LÜ Wei1, LI DongHua2, REN GuoXiang1, ZHANG YanXin2WEN Fei1, HAN JunMei1, ZHANG XiuRong2


  1. 1 Institute of Economic Crops Research, Shanxi Academy of Agricultural Sciences, Taiyuan 030031; 2 Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062
  • Received:2016-08-25 Online:2017-02-16 Published:2017-02-16

Abstract: 【Objective】By association mapping between 33 SSR markers and 18 sesame drought-resistance phenotypes, identifying the associated loci, providing a basis for drought-resistance gene mapping and functional marker development, identifying drought-resistant sesame germplasm resources and providing parental materials and varieties for drought-resistance breeding.【Method】Sesame germplasm populations were cultivated using pots and stressed by drought-water-drought duplication treatment, morphology and growth index during the drought-stress period and mature period were analyzed, then the combination method of SAS, SPSS, and membership function were used for drought-resistance evaluation. At the same time, association analysis was carried out using GLM and MLM models based on molecular markers and drought-resistance phenotype data.【Result】After drought stress, obviously different responses were observed among materials in the population, each index that related to drought resistance averaged smaller than the control; the averaged coefficient of variation of 18 traits was 0.31, which was higher than the control. T test of all traits between treatment and control showed a significant difference. A total of 10 indices related to drought-resistance were screened out, and 12 germplasms with high drought-resistance were identified by functional and clustering analysis. A total of 170 loci were detected by 33 SSR makers in the tested materials, the average loci number was 5.15 per marker. Genetic structure analysis indicated that the population consists of 2 sub-groups. There were 120 loci and 63 loci that were detected by association analysis based on GLM model and MLM model, respectively, the explanation rate ranged from 3.85% to 14.3%, and from 4.00% to 12.5%. There were 12 loci and 3 loci were detected with higher explanation rate than 10% by GLM and MLM, respectively, 5 loci were detected simultaneously by GLM and MLM. By comparison of the primers sequences with sesame genome, 3 genome regions were presumed located genes related with drought-resistance.【Conclusion】This research selected 12 sesame germplasms with high drought-resistance by comprehensive evaluation method, at the same time, 4033-3 and 4033-2 loci that were detected with the highest explanation rate by association analysis based on GLM model and MLM model, respectively, and the explanation rate were 14.3% and 12.5%.

Key words: sesame germplasm, drought resistance, SSR markers, association analysis

[1]    黄凤洪. 花生芝麻加工技术. 北京: 金盾出版社, 1995.
Huang F H. The processing technology of Peanut and sesame. Beijing: Shield press, 1995. (in Chinese)
[2]    Nemeth M, Janda T, Horvath E, Paldi E, Szalai G. Exogenous salicylic acid increases polyamine content but may decrease drought tolerance in maize. Plant Science, 2002, 162: 569-574.
[3]    Fu B Y, Xiong J H, Zhu L H, Zhao X Q, Xu H X, Gao Y M, Li Y S, Xu J L, Li Z K. Identification of functional candidate genes for drought tolerance in rice. Molecular Genetics and Genomics, 2007, 278: 599-609.
[4]    李灿东, 蒋洪蔚, 郭泰, 王志新, 吴秀红, 郑伟, 陈庆山, 胡国华. 大豆耐旱选择群体基因型分析与株高QTL定位. 大豆科学, 2011, 30(1): 15-19.
Li C D, Jiang H W, Guo T, Wang Z X, Wu X H, Zheng W, Chen Q S, Hu G H. QTL identification of plant height and analysis of genotype to soybean in selection population. Soybean Science, 2011, 30(1): 15-19. (in Chinese)
[5]    Pakniyat H, Tavakol E. RAPD markers associated with drought tolerance in bread wheat (Triticum aestivum L.). Pakistan Journal of Biological Sciences, 2007, 10: 3237-3239.
[6]    贺鸿雁, 孙存华, 杜伟, 李扬. PEG 6000胁迫对花生幼苗渗透调节物质的影响. 中国油料作物学报, 2006, 28(1): 76-78.
He H Y, Sun C H, Du W, Li Y. Effects of PEG 6000 osmotic stress on osmolytes of peanut seed-ling. Chinese Journal of Oil Crop Sciences, 2006, 28(1): 76-78. (in Chinese)
[7]    李震, 杨春杰, 张学昆, 邹崇顺, 程勇, 郑普英, 李桂英. PEG胁迫下甘蓝型油菜品种()种子发芽耐旱性鉴定. 中国油料作物学报, 2008, 30(4): 438-442.
Li Z, Yang C J, Zhang X K, Zou C S, Cheng Y, Zheng P Y, Li G Y. Evaluation of drought tolerance in rapeseed (Brassica napus L.) during germination under PEG6000 stress. Chinese Journal of Oil Crop Sciences, 2008, 30(4): 438-442. (in Chinese)
[8]    CARTER T E JR, RUFTY T W, KUO C G. Soybean plant introduction exhibiting drought and aluminum tolerance. Adaptation of food crops to temperature and water stress. Proceedings of an international symposium, Taiwan, 1993: 335-346.
[9]   Songsri P, Jogloy S, Kesmala T, Vorasoot N, Akkasaeng C, Patanothai A. Responses of reproductive characters of drought resistant peanut genotypes to drought. Asian Journal of Plant Sciences, 2008, 7(5): 427-439.
[10]   刘吉利, 赵长星, 吴娜, 王月福, 王铭伦. 苗期干旱及复水对花生光合特性及水分利用效率的影响. 中国农业科学, 2011, 44(3): 469-476.
LIU J L, ZHAO C X, WU N, WANG Y F, WANG M L. Effects of drought and rewatering at seedling stage on photosynthetic characteristics and water use efficiency of peanut. Scientia Agricultura Sinica, 2011, 44(3): 469-476. (in Chinese)
[11]   张智猛, 戴良香, 丁红, 陈殿绪, 杨伟强, 宋文武, 万书波. 中国北方主栽花生品种抗旱性鉴定与评价. 作物学报, 2012, 38(3): 495-504.
ZHANG Z M, DAI L X, DING H, CHEN D X, YANG W Q, SONG W W, WAN S B. Identification and evaluation of drought resistance in different peanut varieties widely grown in Northern China. Acta Agronomica Sinica, 2012, 38(3): 495-504. (in Chinese)
[12]   Norouzi M, Toorchi M, Salekdeh G H, Mohammadi S A, Nishabouri M R, Aharizad S. Effect of water deficit on growth, grain yield and osmotic adjustment in rapeseed. Journal of Food, Agriculture & Environment, 2008, 6(2): 132-138.
[13]   Eslam B P. Evaluation of physiological indices, yield and its components as screening techniques for water deficit tolerance in oilseed rape cultivars. Journal of Agricultural Science and Technology, 2009, 11: 413-422.
[14]   李震, 吴北京, 陆光远, 程勇, 邹崇顺, 张学昆. 不同基因型油菜对苗期水分胁迫的生理响应. 中国油料作物学报, 2012, 34(1): 33-39.
Li Z, Wu B J, Lu G Y, Cheng Y, Zou C S, Zhang X K. Differences in physiological responses of Brassica napus genotypes under water stress during seedling stage. Chinese Journal of Oil Crop Science, 2012, 34(1): 33-39. (in Chinese)
[15]   张霞, 谢小玉. PEG胁迫下甘蓝型油菜种子萌发期抗旱鉴定指标的研究. 西北农业学报, 2012, 21(2): 72-77.
Zhang X, Xie X Y. Studies on identification indexes of drought resistance by PEG during seed germination of rapeseed (Brassica napus L.). Acta Agriculturae Boreali-occidentalis Sinica, 2012, 21(2): 72-77. (in Chinese)
[16]   谢小玉, 张霞, 张兵. 油菜苗期抗旱性评价及抗旱相关指标变化分析. 中国农业科学, 2013, 46(3): 476-485.
Xie X Y, Zhang X, Zhang B. Evaluation of drought resistance and analysis of variation of relevant parameters at seedling stage of rapeseed (Brassica napus L.). Scientia Agricultura Sinica, 2013, 46(3): 476-485. (in Chinese)
[17]   Abbasian A, Rad A H S. Investigation the response of rapeseed cultivars to moisture regimes in different growth stages. Journal of Central European Agriculture, 2011, 12(2): 353-366.
[18]   Mensah J K, Obadoni B O, Eruotor P G, ONOMEIRIEGUNA F. Simulated flooding and drought effects on germination, growth and yield parameters of sesame (Sesamum indicum L.). African Journal of Biotechnology, 2006, 5(13): 1249-1253.
[19]   孙建, 饶月亮, 乐美旺, 颜廷献, 颜小文, 周红英. 干旱胁迫对芝麻生长与产量性状的影响及其抗旱性综合评价. 中国油料作物学报, 2010, 32(4): 525-533.
SUN J, RAO Y L, LE M W, YAN T X, YAN X W, ZHOU H Y. Effects of drought stress on sesame growth and yield characteristics and comprehensive evaluation of drought tolerance. Chinese journal of oil crop sciences, 2010, 32(4): 525-533. (in Chinese)
[20]   唐富福. 水稻农艺和品质若干性状的全基因组初步关联分析[D]. 杭州: 浙江大学, 2013.
Tang F F. Genome-wide association analysis in some agronomic and quality traits of rice[D]. Hangzhou: Zhejiang University, 2013. (in Chinese)
[21]   薛瑞, 周广奇, 胡新文, 郭建春, 唐燕琼. 柱花草种质抗旱性综合评价. 中国农学通报, 2009, 25(11): 224-233.
Xue R, Zhou G Q, Hu X W, Guo J C, Tang Y Q. Comprehensive evaluation of drought resistance in stylosanthes germplasms. Chinese Agricultural Science Bulletin, 2009, 25(11): 224-233. (in Chinese)
[22]   黎冬华, 刘文萍, 张艳欣, 王林海, 危文亮, 高媛, 丁霞, 王蕾, 张秀荣. 芝麻耐旱性的鉴定方法及关联分析. 作物学报, 2013, 39(8): 1425-1433.
LI D H, LIU W P, ZHANG Y X, WANG L H, WEI W L, GAO Y, DING X, WANG L, ZHANG X R. Identification method of drought tolerance and association mapping for sesame (Sesamum indicum L.). Acta Agronomica Sinica, 2013, 39(8): 1425-1433. (in Chinese)
[23]   孙建, 张秀荣, 张艳欣, 王林海, 黄波. 湿害处理对不同生育时期芝麻叶片保护酶活性和种子产量的影响. 应用与环境生物学报, 2009, 15(6): 790-795.
Sun J, Zhang X R, Zhang Y X, WANG L H, HUANG B. Effects of waterlogging on leaf protective enzyme activities and seed yield of sesame at different growth stages. Chinese Journal of Applied & Environmental Biology, 2009, 15(6): 790-795. (in Chinese)
[24]   刘海卿, 孙万仓, 刘自刚, 武军艳. 北方寒旱区白菜型冬油菜抗寒性与抗旱性评价及其关系. 中国农业科学, 2015, 48(18): 3743-3756.
LIU H Q, SUN W C, LIU Z G, WU J Y. Evaluation of drought resistance and cold resistance and research of their relationship at seedling stage of winter rapeseed (Brassica campestris L.) in cold and arid regions in North China. Scientia Agricultura Sinica, 2015, 48(18): 3743-3756. (in Chinese)
[25]   祁旭升, 王兴荣, 许军, 张建平, 米君. 胡麻种质资源成株期抗旱性评价. 中国农业科学, 2010, 43(15): 3076-3087.
Qi X S, Wang X R, Xu J, Zhang J P, Mi J. Drought-resistance evaluation of flax germplasm at adult plant stage. Scientia Agricultura Sinica, 2010, 43(15): 3076-3087. (in Chinese)
[26]   Porebski S, Bailey L G, Baum B R. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Molecular Biology Reporter, 1997, 15: 8-15.
[27]   Zhang Y X, Zhang X Y, Hua W, Wang L H, Che Z. Analysis of genetic diversity among indigenous landraces from sesame (Sesamum indicum L.) core collection in China as revealed by SRAP and SSR markers. Genes and Genomics, 2010, 32: 207-215.
[28]   Kadkhodaie A, Zahedi M, Razmjoo J, Pessarakli M. Changes in some anti-oxidative enzymes and physiological indices among sesame genotypes (Sesamum indicum L.) in response to soil water deficits under field conditions. Acta Physiology Plant, 2014, 36: 641-650.
[29]   Condon A G, Richards R A, Rebetzke G J, Farquhar G D. Breeding for high water use efficiency. Journal of Experimental Botany, 2004, 55: 2447-2460.
[30]   Subrahmanyam D, Subash N, Haris A, Sikka A K. Influence of water stress on leaf photosynthetic characteristics in wheat cultivars differing in their susceptibility to drought. Photosynthetica, 2006, 44: 125-129.
[31]   Subrahmanyam D, Subash N, Haris A, Sikka A K. Influence of water stress on leaf photosynthetic characteristics in wheat cultivars differing in their susceptibility to drought. Photosynthetica, 2006, 44: 125-129.
[32]   朱宗河, 郑文寅, 张学昆. 甘蓝型油菜耐旱相关性状的主成分分析及综合评价. 中国农业科学, 2011, 44(9): 1775-1787.
Zhu Z H, Zheng W Y, Zhang X K. Principal component analysis and comprehensive evaluation on morphological and agronomic traits of drought tolerance in rapeseed (Brassica napus L.). Scientia Agricultura Sinica, 2011, 44(9): 1775-1787. (in Chinese)
[33]   杨万明, 王敏, 李贵全, 杜维俊. PEG胁迫下大豆BIL群体芽期性状与耐旱性评价. 中国油料作物学报, 2013, 35(5): 564-571.
YANG W M, WANG M, LI G Q, DU W J. Germination traits of soybean BIL population under PEG stress and assessment of their drought tolerance Chinese Journal of Oil Crop Sciences, 2013, 35(5): 564-571. (in Chinese)
[34]   贾寿山, 朱俊刚, 王曙光, 史雨刚, 孙黛珍. 山西小麦地方品种萌发期的抗旱性. 华北农学报, 2011, 26(2): 213-217.
JIA S S, ZHU J G, WANG S G, SHI Y G, SUN D Z. Drought resistance of Shanxi wheat landraces at sprouting stage. Acta Agriculturae Boreali-Sinica, 2011, 26(2): 213-217. (in Chinese)
[1] LI ZhouShuai,DONG Yuan,LI Ting,FENG ZhiQian,DUAN YingXin,YANG MingXian,XU ShuTu,ZHANG XingHua,XUE JiQuan. Genome-Wide Association Analysis of Yield and Combining Ability Based on Maize Hybrid Population [J]. Scientia Agricultura Sinica, 2022, 55(9): 1695-1709.
[2] ZHI Lei,ZHE Li,SUN NanNan,YANG Yang,Dauren Serikbay,JIA HanZhong,HU YinGang,CHEN Liang. Genome-Wide Association Analysis of Lead Tolerance in Wheat at Seedling Stage [J]. Scientia Agricultura Sinica, 2022, 55(6): 1064-1081.
[3] MENG Yu,WEN PengFei,DING ZhiQiang,TIAN WenZhong,ZHANG XuePin,HE Li,DUAN JianZhao,LIU WanDai,FENG Wei. Identification and Evaluation of Drought Resistance of Wheat Varieties Based on Thermal Infrared Image [J]. Scientia Agricultura Sinica, 2022, 55(13): 2538-2551.
[4] CHEN Xu,HAO YaQiong,NIE XingHua,YANG HaiYing,LIU Song,WANG XueFeng,CAO QingQin,QIN Ling,XING Yu. Association Analysis of Main Characteristics of Bur and Nut with SSR Markers in Chinese Chestnut [J]. Scientia Agricultura Sinica, 2022, 55(13): 2613-2628.
[5] WANG Juan, MA XiaoMei, ZHOU XiaoFeng, WANG Xin, TIAN Qin, LI ChengQi, DONG ChengGuang. Genome-Wide Association Study of Yield Component Traits in Upland Cotton (Gossypium hirsutum L.) [J]. Scientia Agricultura Sinica, 2022, 55(12): 2265-2277.
[6] YAN YongLiang,SHI XiaoLei,ZHANG JinBo,GENG HongWei,XIAO Jing,LU ZiFeng,NI ZhongFu,CONG Hua. Genome-Wide Association Study of Grain Quality Related Characteristics of Spring Wheat [J]. Scientia Agricultura Sinica, 2021, 54(19): 4033-4047.
[7] JunYi GAI,JianBo HE. Major Characteristics, Often-Raised Queries and Potential Usefulness of the Restricted Two-Stage Multi-Locus Genome-Wide Association Analysis [J]. Scientia Agricultura Sinica, 2020, 53(9): 1699-1703.
[8] JianBo HE,FangDong LIU,WuBin WANG,GuangNan XING,RongZhan GUAN,JunYi GAI. Restricted Two-Stage Multi-Locus Genome-Wide Association Analysis and Its Applications to Genetic and Breeding Studies [J]. Scientia Agricultura Sinica, 2020, 53(9): 1704-1716.
[9] LiYuan PAN,JianBo HE,JinMing ZHAO,WuBin WANG,GuangNan XING,DeYue YU,XiaoYan ZHANG,ChunYan LI,ShouYi CHEN,JunYi GAI. Detection Power of RTM-GWAS Applied to 100-Seed Weight QTL Identification in a Recombinant Inbred Lines Population of Soybean [J]. Scientia Agricultura Sinica, 2020, 53(9): 1730-1742.
[10] ShuGuang LI,YongCe CAO,JianBo HE,WuBin WANG,GuangNan XING,JiaYin YANG,TuanJie ZHAO,JunYi GAI. Genetic Dissection of Protein Content in a Nested Association Mapping Population of Soybean [J]. Scientia Agricultura Sinica, 2020, 53(9): 1743-1755.
[11] ZhiJun XU,Sheng ZHAO,Lei XU,XiaoWen HU,DongSheng AN,Yang LIU. Discovery of Microsatellite Markers from RNA-seq Data in Cultivated Peanut (Arachis hypogaea) [J]. Scientia Agricultura Sinica, 2020, 53(4): 695-706.
[12] WEI Xin, WANG HanTao, WEI HengLing, FU XiaoKang, MA Liang, LU JianHua, WANG XingFen, YU ShuXun. Cloning and Drought Resistance Analysis of GhWRKY33 in Upland Cotton [J]. Scientia Agricultura Sinica, 2020, 53(22): 4537-4549.
[13] LIU ChenXi,WANG BinBin,PU Guang,ZHANG Qian,CAO Yang,WANG Huan,GAO Chen,NIU PeiPei,LI PingHua,HUANG RuiHua. Polymorphism of Rs319699771 Locus of Anti-Diarrhea MUC13 Gene in Suhuai Pig Population and Their Association with Economic Traits [J]. Scientia Agricultura Sinica, 2019, 52(8): 1449-1457.
[14] SUN Kai, LI DongXiu, YANG Jing, DONG JiChi, YAN XianCheng, LUO LiXin, LIU YongZhu, XIAO WuMing, WANG Hui, CHEN ZhiQiang, GUO Tao. Genome-Wide Association Analysis for Rice Submergence Seedling Rate [J]. Scientia Agricultura Sinica, 2019, 52(3): 385-398.
[15] WANG YuBin, PING JunAi, NIU Hao, CHU JianQiang, DU ZhiHong, Lü Xin, LI HuiMing, ZHANG FuYao. Evaluation of Identification and Classification Index for Drought Resistance at Middle and Late Growth Stage in Grain Sorghum Germplasms [J]. Scientia Agricultura Sinica, 2019, 52(22): 4039-4049.
Full text



No Suggested Reading articles found!