Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (16): 3290-3299.doi: 10.3864/j.issn.0578-1752.2014.16.014

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Accumulation of Reactive Oxygen Species Related to Disease Resistance Induced by BABA in Postharvest Banana (Musa AAA. cv. Brazil) Fruit

 TAN  Wei-ping1, 2, PANG  Xue-qun3, ZHANG  Zhao-qi2, HUANG  Xue-mei2   

  1. 1、Guangdong Vocational College of Science and Trade, Guangzhou 510430;
    2、College of Horticulture, South China Agricultural University/Key Laboratory of Postharvest Technology of Fruits and Vegetables in Guangdong Province, Guangzhou 510642;
    3、College of Biotechnology, South China Agricultural University, Guangzhou 510642
  • Received:2014-03-12 Online:2014-08-18 Published:2014-06-23

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Abstract: 【Objective】 Effects of β-aminobutyric acid (BABA) on diseases-tolerance of harvested banana (Musa AAA Group cv. Brazil) and the possible mechanism were investigated to provide theoretical basis for the new technology of resistant and preservation. 【Method】 In this study, diphenylene iodonium (DPI), a NADPH oxidase specific inhibitor, was used to inhibit ROS generation in BABA treated fruits. After 5 g•L-1 BABA treatment or 3.14 mg•L-1DPI followed with 5 g•L-1BABA treatment, banana fruits were inoculated with Collectotrichum musae (2 ? 105 spores/mL) at 0, 3, 6, 12, 24, 48, and 72 h, respectively, and stored at 20±2℃ and RH 85%-95%. The disease spot sizes were determined at 5 d to 16 d. The fruit inoculated with C. musae at 24 h after BABA or DPI treatments and stored at (20±2)℃ and RH85%-95%, and the peel was used for determination of production rate, activities of catalase (CAT) and ascorbate peroxidase (APX), β-1, 3-glucanase(GUN), phenylalanine ammonia lyase(PAL), chitinase (CHI), gene expressions of MaCAT, MaAPX, MaGLU, MaCHI, MaPAL1 and MaNOX during storage. 【Result】 BABA treatment effectively reduced the disease spot sizes on the peel of inoculated banana at 24 h after BABA treatment and cultured for 5 d, showing that BABA treatment needed proper time to have an effect. The production rate and MaNOX gene expression of BABA treated fruits were evidently higher than those of control during 5-12 d storage. Compared with the control, CAT activity and MaCAT expression were higher on day 5 and 1-5 d, respectively. APX activity and MaAPX expression were higher on 5-8 d and 14 d, and 1-5 d, respectively. CHI activity and MaCHI expression was higher on 5-12 d and 1-5 d, respectively. Both of GUN activity and MaGLU expression were higher on 8-14 d. Both PAL activity and MaPAL1 gene expression were higher on 12-14 d . No significant difference was found in other time points. DPI combined with BABA treatment suppressed the above effects of BABA treatment.【Conclusion】These results strongly suggest that reactive oxygen species was involved in the BABA-induced disease tolerance. BABA treatment started the protection mechanism of reactive oxygen species in banana fruits, including the production of reactive oxygen, the increasing of free radical scavenging enzyme activities, and the response of defense-related gene expression.

Key words: banana , fruit , β-aminobutyric acid (BABA) , Collectotrichum musae , reactive oxygen species (ROS) , defense- related enzymes

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