Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (1): 69-79.doi: 10.3864/j.issn.0578-1752.2013.01.009

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Effects of Different Fertilization Regimes on Abundance and Composition of the Bacterial Community in Greenhouse Vegetable Soils

 ZENG  Xi-Bai, WANG  Ya-Nan, WANG  Yu-Zhong, LIN  Zhi-Ling, LI  Lian-Fang, BAI  Ling-Yu, SU  Shi-Ming, SHEN  Ling-Feng   

  1. 1.Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agricultural Environment and Climate Change, Ministry of Agriculture, Beijing 100081
    2.Center of Agricultural Technology Extension, Liangzhou District of Wuwei City, Gansu Province, Wuwei 733000, Gansu
  • Received:2012-04-08 Online:2013-01-01 Published:2012-11-06

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Abstract: 【Objective】 The impacts of different fertilization regimes on the structure and abundance of soil microbial community were studied.【Method】The influence of fertilization regimes including CK, 1/2MNPK, MNPK, M and NPK on the structure community and abundance of the 16S rRNA gene were analyzed by terminal restriction fragment length polymorphism (T-RFLP) and real-time quantitative PCR.【Result】The sequences from 147 positive clones implied that there were ten phylums bacteria in the soil. They were Firmiutes, Proteobacteria, Chloroflexi, Acidobacteria, Bacteroridetes, Actinobacteria, Verrucomicrobia, Cyanbacteria, Nitrospira, and Planctomycetes. Proteobacteria (relative abundance 26.53%), Bacteroridetes (relative abundance 14.97%) and Actinobacteria (relative abundance 10.88%) were dominated diversities in the soil accounted for relative abundance 52.38%. The highest abundance of Shannon, Simpson’s diversity and Margalef Index were all showed in 0-20 cm soil layer of 1/2MNPK treatment with 3.14, 0.945 and 4.31, respectively. The highest Evenness Index was showed in 0-20 cm layer soil of NPK treatment with 0.941. There were pronounced differences in the primary bacterial community composition and abundance in the different fertilization regimes. RDA results indicated that pH (P=0.002) and soil organic matter (SOM, P=0.006) had the greatest influence on the bacterial community composition. The highest abundance of bacterial both in 0-20 cm and 20-40 cm soil layers were detected in 1/2MNPK treatment. The 16S rRNA gene copy numbers in 1/2MNPK regime with 5.26×109 and 4.96×109 copies/g soil for 0-20 cm and 20-40 cm layers soils respectively, which increased by 90.8% and 197.5% compared to the unfertilized regime.【Conclusion】The dominant populations of bacteria in the soil fertilized with organic manure were significantly different from the chemical fertilizer and unfertilized regime. The rational proportion of chemical fertilizer and organic manure (1/2MNPK) can significantly increase the abundance of 16S rRNA gene in the soil.

Key words: greenhouse vegetable soil , fertilization , bacteria , community structure , abundance

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