Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (18): 3543-3555.doi: 10.3864/j.issn.0578-1752.2022.18.006

• PLANT PROTECTION • Previous Articles     Next Articles

Cloning, Expression and Anti-TMV Function Analysis of Nicotiana benthamiana NbMBF1c

YuXia WEN(),Jian ZHANG,Qin WANG,Jing WANG,YueHong PEI,ShaoRui TIAN,GuangJin FAN,XiaoZhou MA,XianChao SUN()   

  1. Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400715
  • Received:2022-04-06 Accepted:2022-05-09 Online:2022-09-16 Published:2022-09-22
  • Contact: SUN XianChao E-mail:wenyuxia123456@163.com;sunxianchao@163.com

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Abstract:

【Objective】Tobacco mosaic virus (TMV) is an important virus that harms crops such as Solanaceae, Cruciferae and Cucurbitaceae, causing great losses to agricultural production. The objective of this study is to obtain Nicotiana benthamiana multiprotein bridging factor 1c (MBF1c) by molecular cloning, clarify the antiviral function and mechanism of NbMBF1c by bioinformatics, cell biology and molecular biology methods, and to provide a theoretical basis for the antiviral breeding of crops.【Method】Based on the full-length NbMBF1c sequence reported in Sol Genomics Network, primers were designed to clone the full-length sequence of NbMBF1c. GeneDoc and MEGA X were employed to align the homologous protein sequences of NbMBF1c and other species. The gene characteristics and protein structure of NbMBF1c were analyzed by bioinformatics. The tissue expression and its expression after TMV infection were detected by real-time fluorescence quantitative PCR (qRT-PCR). Tobacco rattle virus (TRV)-induced gene silencing technology was used to silence NbMBF1c and determine its effect on TMV-GFP infection. The transient overexpression vectors of pART27-GFP-NbMBF1c and pART27-Myc-NbMBF1c were constructed and the fusion protein GFP-NbMBF1c was expressed in N. benthamiana leaf to observe its subcellular localization under confocal microscope. Myc-NbMBF1c was expressed and the changes of TMV-GFP infection after NbMBF1c expressed were observed. qRT-PCR was employed to detect the expression of hormone related genes after NbMBF1c silencing, and the mechanism of NbMBF1c affecting virus infection was analyzed.【Result】NbMBF1c, which is 441 bp in full length, encodes 146 amino acids and contains a conserved domain HTH (helix-turn-helix). Phylogenetic analysis showed that NbMBF1c had the most closely relationship with Nicotiana tomentosiformis MBF1c (XP_009614458.1). NbMBF1c was localized in the cytoplasm and nucleus and had specific tissue expression, with the highest expression in roots, followed by stems, leaves and flowers. Silencing of NbMBF1c in N. benthamiana significantly reduced the tobacco plant height and promoted TMV-GFP infection, on the 5th day after TMV-GFP inoculation, the virus content of new leaf in the silencing treatment was higher than that in the control group. Transient overexpression of Myc-NbMBF1c suppressed TMV-GFP infection, after inoculation with TMV-GFP, virus infection was reduced. However, NbMBF1c did not interact with TMV components, but silencing NbMBF1c up-regulated the expression of abscisic acid (ABA) related gene NCED3, PYL1, ABAI, and SnRK2E and jasmonic acid (JA) synthesis pathway AOS1, while down-regulated the expression of JA signaling pathway receptor COI1.【Conclusion】NbMBF1c is mainly located in cytoplasm and nucleus, and acts as a positive regulator to inhibit TMV infection in N. benthamiana. NbMBF1c does not inhibit viral infection by directly interacting with TMV components, but by regulating phytohormone production and signal transduction.

Key words: Nicotiana benthamiana, NbMBF1c, tobacco mosaic virus (TMV), gene expression, abscisic acid (ABA), jasmonic acid (JA)

Table 1

Primer sequences used in this study"

用途
Use		 引物名称
Primer name		 引物序列F
Primer sequence F (5′-3′)		 引物序列R
Primer sequence R (5′-3′)
实时荧光定量PCR
qRT-PCR		 qNbMBF1c GACGCCGGTTTGAATAAGAA GGCCGTTCATTGATCTGTTT
qTMV-GFP GACCTGACAAAAATGGAGAAGATCT GAAAGCGGACAGAAACCCGCTG
qNbNCED3 ACCCGACTCGATTTTCAATG AACTTTTGGCCATGGTTCTG
qNbAOS1 CATGAAAGATTTGCCGGTTT ACTTGCAAGCATCAGCAATG
qNbPYL1 CAGGGTTGACACCAGAA TGTGCGAGTAGGGAAGA
qNbSnRK2E TTGCCACCGAGACTTGA CTGCGAATGTAACACTGAGGA
qNbABAI1 GCCGTTGTTTGTTCATCTC ACTGTATCACCTTGCCTCC
qNbCOl 1 CAGCAGCCCATTGTTTCTTAC TACTGGCCAAGTACTTCCAATC
qNbPR1 ATGGTCAATACGGCGAAAAC CCTAGCACATCCAACACGAA
qNbActin CTTGAAACAGCAAAGACCAGC CATCCTATCAGCAATGCCCG
沉默载体构建
Construction of silent vector		 TRV-NbMBF1c GCTCTAGAGCCGGTCCGACCAACT CCGCTCGAGTTCTAACATTAACGACAGCG
瞬时表达载体构建Construction of transient overexpression vectors GFP-NbMBF1c CGGAATTCATGCCGGTCCGACCAACT GCTCTAGATCACTTGTGAATTTTACCTC
Myc-NbMBF1c CGGAATTCATGCCGGTCCGACCAACT GCTCTAGATCACTTGTGAATTTTACCTC
酵母载体构建Construction of yeast-expression vectors
AD-NbMBF1c CCGGAATTCATGCCGGTCCGACCAACT CGCGTCGACTCACTTGTGAATTTTACCTC
BK-NbMBF1c CCGGAATTCATGCCGGTCCGACCAACT CGCGTCGACTCACTTGTGAATTTTACCTC
BK-TMV CP GCGAATTCATGTCTTACAGTATCACTAC CCGCTCGAGTCAAGTTGCAGGACCAGAGG
AD-TMV P50 CATGGAGGCCGAATTGAATTCATGGAGATAGAGTCTTTAGAGCAGTT GCCGCTGCAGGTCGAGTCGACCTATTGTGTTCCTGCA
BK-TMV MP GGAATTCCATATGCAATGACTCATTCTCATGAACCATTATTTTATCAACT CGGGATCCAAACGAATCC

Fig. 1

Amino acid sequence alignment and phylogenetic analysis of NbMBF1c and MBF1c from other plants NbMBF1c and its homologous phylogenetic tree, the numbers on the branches represented the values of Bootstrap test, the short line below indicated the distance scale 0.050"

Fig. 2

Expression analysis of NbMBF1cNbMBF1c expression after TMV infection. The statistical analyses were performed using Student’s t-test (***P<0.001). The experiments were repeated three times with three plants each time. Values represent means±SE from three biological replications"

Fig. 3

Subcellular localization of NbMBF1c and localization change of NbMBF1c after TMV infection eGFP:NbMBF1c with mCherry:00 and mCherry:H2B were transiently co-expressed in the epidermal cells of N. benthamiana leaves by agroinfiltration. mCherry and GFP fluorescence signals were visualized using laser confocal microscope at 48 h after infiltration and were depicted in red and green, respectively. Pearson’s coefficient of localization represents fluorescence coincidence degree, the intensity of GFP fluorescence (green line) and mCherry fluorescence (red line) were presented in the right panels. The white scale bars represented 20 μm; 313Location of eGFP:NbMBF1c after TMV infection"

Fig. 4

Phenotype and silencing efficiency detection in NbMBF1c silenced plant Silencing efficiency detection in TRV:NbMBF1c. The statistical analyses were performed using Student’s t-test (*0.01<P<0.05, ***P<0.001). The experiments were repeated three times with ten plants each time. Values represent means±SE from three biological replications"

Fig. 5

Silencing of NbMBF1c promotes TMV-GFP infection Detection of TMV-GFP content in TRV:NbMBF1c and TRV:00. The statistical analyses were performed using Student’s t-test (**0.001<P<0.01, ***P<0.001). The experiments were repeated three times with ten plants each time. Values represent means±SE from three biological replications"

Fig. 6

Overexpression of NbMBF1c inhibits TMV-GFP infection"

Fig. 7

The interaction of NbMBF1c and TMV virus components was analyzed by Y2H"

Fig. 8

Effect of NbMBF1c silencing on expression of ABA and JA related genes Actin is employed as an internal reference. The statistical analyses were performed using Student’s t-test (*0.01<P<0.05, **0.001<P<0.01, ns means no significant difference). The experiments were repeated three times with ten plants each time. Values represent means±SE from three biological replications"

[1] 侯红琴, 谭志琼, 张振臣. 烟草花叶病毒属研究新进展. 中国农学通报, 2008, 24(5): 304-307.
HOU H Q, TAN Z Q, ZHANG Z C. Research advances in Tobamovirus. Chinese Agricultural Science Bulletin, 2008, 24(5): 304-307. (in Chinese)
[2] SUZUKI N, BAJAD S, SHUMAN J, SHULAEV V, MITTLER R. The transcriptional co-activator MBF1c is a key regulator of thermotolerance in Arabidopsis thaliana. The Journal of Biological Chemistry, 2008, 283(14): 9269-9275.
doi: 10.1074/jbc.M709187200
[3] JAIMES-MIRANDA F, MONTES R. The plant MBF1 protein family: A bridge between stress and transcription. Journal of Experimental Botany, 2020, 71(6): 1782-1791.
doi: 10.1093/jxb/erz525
[4] TAKEMARU K I, HARASHIMA S, UEDA H, HIROSE S. Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation. Molecular and Cellular Biology, 1998, 18(9): 4971-4976.
doi: 10.1128/MCB.18.9.4971
[5] OZAKI J, TAKEMARU K, IKEGAMI T, MISHIMA M, UEDA H, HIROSE S, KABE Y, HANDA H, SHIRAKAWA M. Identification of the core domain and the secondary structure of the transcriptional coactivator MBF1. Genes to Cells, 1999, 4(7): 415-424.
doi: 10.1046/j.1365-2443.1999.00267.x
[6] SUZUKI N, RIZHSKY L, LIANG H, SHUMAN J, SHULAEV V, MITTLER R. Enhanced tolerance to environmental stress in transgenic plants expressing the transcriptional coactivator multiprotein bridging factor 1c (MBF1c). Plant Physiology, 2005, 139: 1313-1322.
doi: 10.1104/pp.105.070110
[7] SUZUKI N, SEJIMA H, TAM R, SCHLAUCH K, MITTLER R. Identification of the MBF1 heat-response regulon of Arabidopsis thaliana. The Plant Journal, 2011, 66(5): 844-851.
doi: 10.1111/j.1365-313X.2011.04550.x
[8] GARG A K, KIM J K, OWENS T G, RANWALA A P, CHOI Y D, KOCHIAN L V, WU R J. Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99(25): 15898-15903.
[9] PENNA S. Building stress tolerance through over-producing trehalose in transgenic plants. Trends in Plant Science, 2003, 8(8): 355-357.
doi: 10.1016/S1360-1385(03)00159-6
[10] KIM M J, LIM G H, KIM E S, KO C B, YANG K Y, JEONG J A, LEE M C, KIM C S. Abiotic and biotic stress tolerance in Arabidopsis overexpressing the multiprotein bridging factor 1a (MBF1a) transcriptional coactivator gene. Biochemical and Biophysical Research Communications, 2007, 354(2): 440-446.
doi: 10.1016/j.bbrc.2006.12.212
[11] LV X, XIANG S, WANG X, WU L, LIU C, YUAN M, GONG W, WIN H, HAO C, XUE Y, MA L, CHENG D, SUN X. Synthetic chloroinconazide compound exhibits highly efficient antiviral activity against tobacco mosaic virus. Pest Management Science, 2020, 76(11): 3636-3648.
doi: 10.1002/ps.5910
[12] LIU C, TIAN S, LV X, PU Y, PENG H, FAN G, MA X, MA L, SUN X. Nicotiana benthamiana asparagine synthetase associates with IP-L and confers resistance against tobacco mosaic virus via the asparagine-induced salicylic acid signalling pathway. Molecular Plant Pathology, 2022, 23(1): 60-77.
doi: 10.1111/mpp.13143
[13] 刘昌云, 李欣羽, 田绍锐, 王靖, 裴悦宏, 马小舟, 樊光进, 汪代斌, 孙现超. 番茄SlN-like的克隆、表达与抗病毒功能. 中国农业科学, 2021, 54(20): 4348-4357.
LIU C Y, LI X Y, TIAN S R, WANG J, PEI Y H, MA X Z, FAN G J, WANG D B, SUN X C. Cloning, expression and anti-virus function analysis of Solanum lycopersicum SlN-like. Scientia Agricultura Sinica, 2021, 54(20): 4348-4357. (in Chinese)
[14] LIU C, PU Y, PENG H, LV X, TIAN S, WEI X, ZHANG J, ZOU A, FAN G, SUN X. Transcriptome sequencing reveals that photoinduced gene IP-L affects the expression of PsbO to response to virus infection in Nicotiana benthamiana. Physiological and Molecular Plant Pathology, 2021, 114: 101613.
doi: 10.1016/j.pmpp.2021.101613
[15] YU R M, SUO Y Y, YANG R, CHANG Y N, TIAN T, SONG Y J, WANG H J, WANG C, YANG R J, LIU H L, GAO G. StMBF1c positively regulates disease resistance to Ralstonia solanacearum via it’s primary and secondary upregulation combining expression of StTPS5 and resistance marker genes in potato. Plant Science, 2021, 307: 110877.
doi: 10.1016/j.plantsci.2021.110877
[16] QIN D, WANG F, GENG X, ZHANG L, YAO Y, NI Z, PENG H, SUN Q. Overexpression of heat stress-responsive TaMBF1c, a wheat (Triticum aestivum L.) multiprotein bridging factor, confers heat tolerance in both yeast and rice. Plant Molecular Biology, 2015, 87(1): 31-45.
doi: 10.1007/s11103-014-0259-9
[17] 唐平华, 陈国平, 潘宇, 张彦杰, 张彬, 胡宗利. 番茄多蛋白桥梁因子基因LeMBF1的克隆及抗病性分析. 生命科学研究, 2012, 16(2): 138-143, 148.
TANG P H, CHEN G P, PAN Y, ZHANG Y J, ZHANG B, HU Z L. Cloning and analysis of pathogen resistance of multiprotein bridging factor gene LeMBF1 in tomato. Life Science Research, 2012, 16(2): 138-143, 148. (in Chinese)
[18] 曹兴, 郭尚敬, 高祥斌, 吕福堂, 隋娟娟, 穆红梅, 吴泽, 义鸣放, 张秀省. 百合转录辅激活因子LlMBF1c基因的克隆与表达分析. 江苏农业学报, 2018, 34(5): 1120-1127.
CAO X, GUO S J, GAO X B, LÜ F T, SUI J J, MU H M, WU Z, YI M F, ZHANG X S. Cloning and expression analysis of LlMBF1c in lily. Jiangsu Journal of Agricultural Sciences, 2018, 34(5): 1120-1127. (in Chinese)
[19] SALGADO-BLANCO D, LÓPEZ-URÍAS F, OVANDO-VÁZQUEZ C, JAIMES-MIRANDA F. DNA-MBF1 study using molecular dynamics simulations. On the road to understanding the heat stress response in DNA-protein interactions in plants. European Biophysics Journal, 2021, 50(8): 1055-1067.
doi: 10.1007/s00249-021-01565-x
[20] TSUDA K, YAMAZAKI K I. Structure and expression analysis of three subtypes of Arabidopsis MBF1 genes. Biochimica et Biophysica Acta, 2004, 1680(1): 1-10.
[21] WANG X, DU Y, YU D. Trehalose phosphate synthase 5-dependent trehalose metabolism modulates basal defense responses in Arabidopsis thaliana. Journal of Integrative Plant Biology, 2019, 61(4): 509-527.
doi: 10.1111/jipb.12704
[22] 张毅, 张岗, 董艳玲, 郭军, 黄丽丽, 康振生. 条锈菌诱导的小麦MBF1转录辅激活因子基因的克隆及其特征分析. 作物学报, 2009, 35(1): 11-17.
doi: 10.3724/SP.J.1006.2009.00011
ZHANG Y, ZHANG G, DONG Y L, GUO J, HUANG L L, KANG Z S. Cloning and characterization of a MBF1 transcriptional coactivator factor in wheat induced by stripe rust pathogen. Acta Agronomica Sinica, 2009, 35(1): 11-17. (in Chinese)
doi: 10.3724/SP.J.1006.2009.00011
[23] MATSUSHITA Y, MIYAKAWA O, DEGUCHI M, NISHIGUCHI M, NYUNOYA H. Cloning of a tobacco cDNA coding for a putative transcriptional coactivator MBF1 that interacts with the tomato mosaic virus movement protein. Journal of Experimental Botany, 2002, 53(373): 1531-1532.
doi: 10.1093/jexbot/53.373.1531
[24] NAMBARA E, MARION-POLL A. Abscisic acid biosynthesis and catabolism. Annual Review of Plant Biology, 2005, 56: 165-185.
doi: 10.1146/annurev.arplant.56.032604.144046
[25] GUO W, CHEN R, DU X, ZHANG Z, YIN Y, GONG Z, WANG G. Reduced tolerance to abiotic stress in transgenic Arabidopsis overexpressing a Capsicum annuum multiprotein bridging factor 1. BMC Plant Biology, 2014, 14: 138.
doi: 10.1186/1471-2229-14-138
[26] ALAZEM M, LIN N S. Interplay between ABA signaling and RNA silencing in plant viral resistance. Current Opinion in Virology, 2020, 42: 1-7.
doi: 10.1016/j.coviro.2020.02.002
[27] WANG L, WU Y, TIAN Y, DAI T, XIE G, XU Y, CHEN F. Overexpressing Jatropha curcas CBF2 in Nicotiana benthamiana improved plant tolerance to drought stress. Gene, 2020, 742: 144588.
doi: 10.1016/j.gene.2020.144588
[28] XIE K, LI L, ZHANG H, WANG R, TAN X, HE Y, HONG G, LI J, MING F, YAO X, YAN F, SUN Z, CHEN J. Abscisic acid negatively modulates plant defence against rice black-streaked dwarf virus infection by suppressing the jasmonate pathway and regulating reactive oxygen species levels in rice. Plant, Cell and Environment, 2018, 41(10): 2504-2514.
doi: 10.1111/pce.13372
[29] OKA K, KOBAYASHI M, MITSUHARA I, SEO S. Jasmonic acid negatively regulates resistance to tobacco mosaic virus in tobacco. Plant and Cell Physiology, 2013, 54(12): 1999-2010.
doi: 10.1093/pcp/pct137
[30] LIU Y, SCHIFF M, DINESH-KUMAR S P. Involvement of MEK1 MAPKK, NTF6 MAPK, WRKY/MYB transcription factors, COI1 and CTR1 in N-mediated resistance to tobacco mosaic virus. The Plant Journal, 2004, 38(5): 800-809.
doi: 10.1111/j.1365-313X.2004.02085.x
[31] ZHU F, XI D H, YUAN S, XU F, ZHANG D W, LIN H H. Salicylic acid and jasmonic acid are essential for systemic resistance against tobacco mosaic virus in Nicotiana benthamiana. Molecular Plant- Microbe Interactions, 2014, 27(6): 567-577.
doi: 10.1094/MPMI-11-13-0349-R
[32] ATTARAN E, ZEIER T E, GRIEBEL T, ZEIER J. Methyl salicylate production and jasmonate signaling are not essential for systemic acquired resistance in Arabidopsis. The Plant Cell, 2009, 21(3): 954-971.
doi: 10.1105/tpc.108.063164
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