IGF1-PI3K-Akt信号通路相关基因在黄羽肉鸡肌肉和肝脏中的表达

doi:10.3864/j.issn.0578-1752.2021.09.018

Abstract

Abstract:

【Objective】IGF1-PI3K-Akt signal pathway have been implicated in the regulation of growth and development of chicken. In the present study, the expression of IGF, IGF1R and IRS1genes of IGF1-PI3K-Akt signal pathway was first studied in skeletal muscle and liver tissues of Guangxi partridge chickens with slow growth rate and Huashan partridge chickens with moderate growth rate, which would provide a reference for clarifying the regulation mechanism of growth and development of yellow feather broilers. 【Method】SPSS20.0 software was used to compare the differences of growth traits between Guangxi partridge chickens and Huashan partridge chickens. Expression of IGF1, IGF1R and IRS1 was quantified by RT-PCR in the breast muscle (BM), leg muscle (LM) and liver on days 9, 12, and 16 of embryonic development, as well as at 0, 7, 21, 35, 49, and 63 days post-hatching (PH) in the two chicken breeds, and the correlations between the gene expression level and body weight as well as tissue weight were also analyzed by SPSS20.0 software. 【Result】The body weight, skeletal muscle and liver weight of chicken in the early growth development showed significant breed- and age-specificity. The growth of Huashan partridge chickens had far exceeded that of Guangxi partridge chickens from the late embryonic development. The expression patterns of IGF1, IGF1R and IRS1 showed significant differences in breed-, tissue- and age-specific fashion. Overall, the expression levels of the three genes in skeletal muscles of embryonic development were much higher than those in liver; in contrast, the expression levels in liver were higher than those in skeletal muscle at post-hatching development. As for breed, expression levels of the studied genes in skeletal muscle of Guangxi partridge chickens were higher than those in Huashan partridge chickens; in contrast, liver expression levels were higher in Huashan partridge chickens. IGF1 mRNA could be detected as early as on E9 d in the skeletal muscles of both chicken breeds, and the highest level was appeared at this stage in LM, significantly higher than those on the other studied development stages expect on E12 d, and the lowest level was appeared on 0 d at hatching; while in BM, the highest level was appeared on E12 d and lowest level was appeared on 63 d; however, the level of IGF1 mRNA differed between breeds in liver. For Guangxi partridge chickens, the highest level was observed on 21 d and there was no expression during embryonic development, whereas the highest level was found on 49 d and very low level during embryonic development in Huashan partridge chickens. IGF1R mRNA could be detected at the whole studied stages in skeletal muscles and liver of both chicken breeds, and the highest level was appeared on E9 d in both BM and LM, significantly higher than those on the other studied development stages in Guangxi partridge chickens, and significantly higher than those on the other studied development stages expect on E12 d in Huashan partridge chickens. In liver, the highest expression level of IGF1R was detected on 21 d in Guangxi partridge chickens, significantly higher than those on E9 d, E12 d, E16 d and 0 d; while the highest level was found on 63 d and significantly higher than those on the other studied development stages. IRS1 mRNA could also be detected at the whole studied stages in skeletal muscles and liver of both chicken breeds. In skeletal muscles, expression of IRS1 were higher on E9 d or E12 d than any other age, and then decreased with age and kept relative low level during PH development in both breeds. The liver IRS1 expression pattern was consistent with IGF1R in both breeds. Significant positive relationships were observed for the expression of studied genes in BM, LM and liver tissues of both chicken breeds. Meanwhile, the skeletal muscle expression of these three genes were all showed significant negative relationships with body weight and breast (leg) muscle weight, whereas the liver expression of these three genes were all showed significant positive relationships with body weight, breast (leg) muscle weight and liver weight. 【Conclusion】These results implied that the expression of selected genes that comprise the IGF1-PI3K-Akt pathway existed identical trends, and differential expression between breeds and tissues might be one of the main reasons for the different growth rate of the different types of yellow feather broiler.

Key words: yellow feather broiler, gene expression, IGF1-PI3K-Akt pathway, skeletal muscle, liver

SHU JingTing,JI GaiGe,SHAN YanJu,ZHANG Ming,JU XiaoJun,LIU YiFan,TU YunJie,SHENG ZhongWei,TANG YanFei,JIANG HuaLian,ZOU JianMin. Expression Analysis of IGF1-PI3K-Akt-Dependent Pathway Genes in Skeletal Muscle and Liver Tissue of Yellow Feather Broilers[J].Scientia Agricultura Sinica, 2021, 54(9): 2027-2038.

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Figures/Tables 5

Table 1

Fig. 1

Table 2

The relative expression level of IGF1, IGF1R and IRS1 in skeletal muscles and liver of Huashan and Guangxi partridge chickens at different developmental stages"

基因Gene	组织Tissue	品种 Breed	9 ed	12 ed	16 ed	0 d	7 d	21 d	35 d	49 d	63 d
IGF1	胸肌 Breast	GXMJ	9.65±1.06 a	9.78±0.37a	5.18±1.07b	1.51±0.33 c	0.27±0.08d	0.19±0.05d	0.14±0.03d	0.06±0.005e	0.05±0.005e
	胸肌 Breast	HSMJ	1.12±0.18b**	1.92±0.26a**	0.98±0.26b**	0.42±0.07c*	0.24±0.08c	0.18±0.05c	0.17±0.03c	0.13±0.04c	0.05±0.004d
	腿肌 Leg	GXMJ	54.92±2.51a	54.77±5.06a	33.20±3.39 b	1.32±0.39c	2.08±0.37cd	2.86±0.44d	2.51±0.48cd	3.42±0.85d	1.58±0.16c
	腿肌 Leg	HSMJ	10.44±1.5a**	10.31±0.2a**	1.77±0.20b**	0.26±0.65c*	2.74±0.57d	1.53±0.26b	3.79±0.86d	2.25±0.42b	1.06±0.18b
	肝脏 Liver	GXMJ	0	0	0	1.23±0.23c	4.02±1.07c	57.62±6.46a	47.84±5.54a	52.47±7.05a	29.81±3.59b
	肝脏 Liver	HSMJ	0.04±0.01e*	0.22±0.02d*	0.31±0.04d	3.02±0.52c*	5.11±1.47c	66.06±6.99b*	74.44±9.4ab*	80.87±12.0a*	79.71±9.1a**
IGF 1R	胸肌 Breast	GXMJ	3.50±0.23 a	1.64±0.15b	2.63±0.33b	1.32±0.33 c	0.06±0.01d	0.08±0.01d	0.10±0.02d	0.03±0.00d	0.04±0.00d
	胸肌 Breast	HSMJ	0.45±0.07a**	0.24±0.04b**	0.25±0.06b**	0.17±0.07bc*	0.02±0.00c	0.02±0.00c	0.05±0.00c	0.03±0.00c	0.01±0.00c
	腿肌 Leg	GXMJ	6.14±0.86a	4.21±0.56b	2.31±0.37c	1.18±0.28d	0.46±0.08d	0.37±0.05d	0.49±0.05d	0.30±0.04d	0.21±0.06d
	腿肌 Leg	HSMJ	1.24±0.23a**	1.21±0.27a*	0.18±0.05b**	0.10±0.01b**	0.17±0.02b	0.12±0.02b	0.29±0.06b	0.17±0.02b	0.17±0.01b
	肝脏 Liver	GXMJ	0.15±0.02c	0.16±0.02 c	0.37±0.03c	0.96±0.06b	1.62±0.19ab	1.96±0.27a	1.48±0.16ab	1.54±0.19ab	1.35±0.28ab
	肝脏 Liver	HSMJ	0.21±0.05d	0.36±0.04d	0.50±0.03d	1.21±0.18c	1.77±0.23bc	1.78±0.30bc	1.88±0.24bc	2.60±0.35b*	5.74±0.91a*
IRS1	胸肌 Breast	GXMJ	2.07±0.09a	1.69±0.12a	1.51±0.16a	1.50±0.13a	0.17±0.02b	0.30±0.06b	0.37±0.03b	0.22±0.03b	0.21±0.02b
	胸肌 Breast	HSMJ	0.56±0.05a**	0.39±0.03a*	0.31±0.02a*	0.55±0.06a*	0.09±0.01c	0.16±0.01b	0.21±0.03b	0.13±0.04b	0.02±0.00c*
	腿肌 Leg	GXMJ	5.84±0.59a	4.32±0.49b	1.93±0.11c	1.51±0.04cd	0.79±0.06cd	1.07±0.13cd	0.90±0.10cd	0.80±0.06cd	0.61±0.05d
	腿肌 Leg	HSMJ	2.08±0.26a**	2.28±0.54a*	0.63±0.10b*	0.73±0.04b*	0.26±0.04b*	0.40±0.06b*	0.60±0.09b	0.48±0.07b	0.38±0.04b
	肝脏 Liver	GXMJ	0.09±0.00c	0.22±0.04c	0.10±0.01c	0.92±0.20b	1.80±0.27ab	3.08±0.46a	2.84±0.55a	3.02±0.74a	2.63±0.22a
	肝脏 Liver	HSMJ	0.08±0.00f	0.11±0.01f	0.08±0.01f	0.76±0.13e	1.52±0.11e	2.58±0.15d	3.53±0.53c	6.29±0.73b*	16.05±2.01a**

Table 2

Table 3

Table 4

References 30

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参数 Parameters		广西麻鸡Guangxi Partridge		花山麻鸡Huashan Partridge
参数 Parameters		IGF1R	IRS1	IGF1R	IRS1
胸肌 Breast muscle	IGF1	0.851**	0.641**	0.418**	0.457**
胸肌 Breast muscle	IRS1	0.806**		0.648**
腿肌 Leg muscle	IGF1	0.884**	0.873**	0.719**	0.670**
腿肌 Leg muscle	IRS1	0.854**		0.884**
肝脏 Liver	IGF1	0.494**	0.682**	0.489**	0.389**
肝脏 Liver	IRS1	0.759**		0.911**

组织 Tissue	参数 Parameters	广西麻鸡Guangxi Partridge			花山麻鸡Huashan Partridge
组织 Tissue	参数 Parameters	IGF1	IGF1R	IRS1	IGF1	IGF1R	IRS1
胸肌 Breast muscle	体重Body weight	-0.453**	-0.537**	-0.467**	-0.519*	-0.430**	-0.594**
胸肌 Breast muscle	胸肌重Breast weight	-0.405**	-0.483**	-0.424**	-0.441**	-0.361**	-0.542**
腿肌 Leg muscle	体重Body weight	-0.413**	-0.471**	-0.451**	-0.389**	-0.332**	-0.370**
腿肌 Leg muscle	腿肌重Leg weight	-0.377**	-0.435**	-0.417**	-0.362**	-0.284*	-0.321**
肝脏 Liver	体重Body weight	0.369*	0.965**	0.817**	0.693**	0.781**	0.817**
	肝脏重Liver weight	0.458**	0.409**	0.851**	0.601**	0.794**	0.851**
	胸肌重Breast weight	0.352**	0.453**	0.783**	0.563**	0.696**	0.752**
	腿肌重Leg weight	0.365**	0.483**	0.812**	0.598**	0.723**	0.795**

Expression Analysis of IGF1-PI3K-Akt-Dependent Pathway Genes in Skeletal Muscle and Liver Tissue of Yellow Feather Broilers

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基因名称 Gene name	引物序列 Primer sequence	PCR条件 PCR conditions (℃)
IGF1	5′ primer: AGCTGGTTGATGCTCTTCAGTTCGT	60
IGF1	3′ primer: TCCTCAGGTCACAACTCTGGAAGC	60
IGF1R	5′ primer: TTCAGGAACCAAAGGGCGA	55
IGF1R	3′ primer: TGTAATCTGGAGGGCGATACC	55
IRS1	5′ primer: CTACCGCCTGTGCCTGACTAAC	60
IRS1	3′ primer: CGTCCCACCTCGATGAAGAAG	60
HSP70	5′ primer: TCTGCTCCTGTTGGATGTC	60
HSP70	3′ primer: TGGGAATGGTGGTGTTACG	60

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