Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (9): 1846-1855.doi: 10.3864/j.issn.0578-1752.2021.09.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Influence of Anthocyanin Biosynthesis on Leaf and Fiber Color of Gossypium hirsutum L.

YUAN JingLi(),ZHENG HongLi,LIANG XianLi,MEI Jun,YU DongLiang,SUN YuQiang,KE LiPing()   

  1. School of Life Sciences and Medicine, Zhejiang Sci-Tech University/Plant Genomics & Molecular Improvement of Colored Fiber Lab, Hangzhou 310018
  • Received:2020-10-04 Accepted:2020-12-25 Online:2021-05-01 Published:2021-05-10
  • Contact: LiPing KE E-mail:yuanjingli2017@126.com;keliping@zstu.edu.cn

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Abstract:

【Objective】Cotton is an important economic and oil crop. Both its leaves and fibers can accumulate pigments and present different colors. Studies have confirmed that chlorophyll, carotenoids, and anthocyanin are the main pigments in cotton leaves and their relative proportion changes leaf colors. While proanthocyanidins and their derivatives, which are oligomeric and polymeric products from anthocyanidins, are thought to be responsible for the color formation of brown fibers. This article intends to explore the relationship between the color of leaves and fibers in upland cotton through analyzing the anthocyanidin content and gene expression level in the anthocyanin biosynthesis pathway in different leaf color mutants. The result will help to lay the foundation for the utilization of leaf color mutants and the improvement of the color of colored cotton fibers. 【Method】In this experiment, the anthocyanidin contents in leaves of 21 upland cotton leaf color mutants were detected. According to the leaf and fiber color as well as the anthocyanidin content level, 6 cotton leaf color mutants were selected as materials to measure the anthocyanidin level in leaves and fibers at 15 days post anthesis to analyze the relationship between anthocyanidin content and the leaf or fiber color. Then the expression levels of GhCHS, GhLAR and GhANR in leaves and fibers at different developmental stages (5, 10, 15, 20 DPA) were measured to analyze the influence of target genes on the color formation of leaves and fibers. 【Result】The anthocyanidin content in the leaves of 21 leaf color mutants of G. hirsutum was significantly different, and the purple or fuchsia leaves had higher anthocyanidin content. In the selected six leaf color mutants, the anthocyanidin content in leaves was significantly higher than that in fibers, and brown fibers accumulated more anthocyanidins than white fibers. Compared to GhCHS, GhANR and GhLAR expressed at a very low level in leaves, and no significant correlation was found between leaf color and their expression level. While in fibers, the expressions of GhANR and GhLAR were obviously higher in brown fibers than in white fibers, especially in fibers of 5 DPA to 15 DPA. 【Conclusion】Anthocyanins played important roles in color formation of both leaves and fibers in upland cotton. Purple-red or purple leaves and brown fibers accumulated more anthocyanidins, while the formation of fiber color did not directly correlate with leaf color. In fibers, the contents of anthocyanidins directly related to the expression levels of GhANR and GhLAR, indicating that coloration mechanism of cotton leaves and fibers was not exactly the same, and proanthocyanidins mainly accumulated in fibers.

Key words: Gossypium hirsutum L., leaf color mutant, fiber color, anthocyanin, gene expression

Table 1

Experimental materials"

样本编号
ID		 名称
Name		 原产地
Place of origin		 叶色
Leaf color		 纤维颜色
Fiber color
1 红叶白絮 Hongyebaixu 中国山西省 Shanxi Province, China 紫 Purple 白 White
2 石河子822 Shihezi 822 中国新疆建设兵团Xinjiang Construction Corps, China 绿/紫Green/purple 白 White
3 送兴红叶B Songxinghongye B 中国河北Hebei, China 红Red 白 White
4 红叶棕絮 Hongyezongxu 中国山西Shanxi, China 绿Green 棕 Brown
5 锦9-70 Jin 9-70 中国辽宁Liaoning, China 绿Green 白 White
6 紫花棉 Zihuamian 中国辽宁Liaoning, China 绿/紫Green/purple 棕 Brown
7 红叶鸡脚 Hongyejijiao 中国四川Sichuan, China 红Red 白 White
8 安徽红桃棉 Hongtao 中国安徽Anhui, China 红Red 白 White
9 LA877 美国 U.S.A 绿/红Green/purple 白 White
10 抗红叶 Kanghongye 中国四川Sichuan, China 红Red 白 White
11 小红叶 Xiaohongye 中国河南Henan, China 红Red 白 White
12 红叶花苞棉 Hongyehuabaomian 中国河南Henan, China 红Red 白 White
13 矮红株 Aihongzhu 中国江苏Jiangsu, China 紫Purple 白 White
14 芽黄棉1号 Yahuangmian1 中国湖北Hubei, China 黄/绿Yellow/green 白 White
15 花斑叶Huabanye 中国河南Henan, China 斑驳Mottled leaf 白 White
16 斑叶棉 Banyemian 中国河南Henan, China 斑驳Mottled leaf 白 White
17 红鸡脚柳苞 Hongjijiaoliubao 中国贵州 Guizhou, China 紫Purple 白 White
18 红槿矮 Hongjinai 中国湖北 Hubei, China 绿Green 白 White
19 观赏棉120 Guanshangmian120 中国河北Hebei, China 中红Middle red 白 White
20 贵池红叶 Guichihongye 中国安徽Anhui, China 紫Purple 白 White
21 HS2 中国浙江Zhejiang, China 紫Purple 白 White

Table 2

Primers and sequence used in this experiment"

引物名称
Primer name		 序列
Primer sequence (5′-3′)		 用途
Use
T-GhCHS-F GCTGTTACCTTTCGTGGACC 基因表达分析 Gene expression analysis
T-GhCHS-R TCGCACCATCACTATCTGGC
T-GhANR-F GACGTTGGCTGAAAAGGCAG 基因表达分析 Gene expression analysis
T-GhANR-R CAGAAAAACATGGGCTCGGC
T-GhLAR-F GCATCTTGGACCAGCTCAGT 基因表达分析 Gene expression analysis
T-GhLAR-R GTGTTGTCATGGTAGGGCCA
T-GhUBQ7-F GAAGGCATTCCACCTGACCAAC 内参基因 Reference gene
T-GhUBQ7-R CTTGACCTTCTTCTTCTTGTGCTTG

Fig. 1

Anthocyanins extraction solution of the leaf color mutants of G. hirsutum L. The sample ID is the same as table 1. The same as below"

Fig. 2

Scanning of anthocyanins extraction solution in the leaf color mutants of G. hirsutum L. According to the absorption value at light wavelength 530 nm, the sample ID from top to bottom is 20, 21, 17, 13, 7, 1, 4, 15, 10, 19, 11, 3, 2, 18, 12, 14, 6, 16, 5, 9, 8"

Fig. 3

The anthocyanin content of leaves in the leaf color mutants of G. hirsutum L. Compared with C312, the asterisk showed significant difference (*P<0.05, **P<0.01). The same as below"

Fig. 4

The phenotypic analysis in the leaf and fiber color of G. hirsutum L. A: Phenotypic analysis in the leaf color of G. hirsutum L.; B: Phenotypic analysis in the fiber color of G. hirsutum L."

Fig. 5

Comparison of anthocyanin content of leaves and fibers (15DPA) in the different leaf color mutants of G. hirsutum L. A: Anthocyanin content in leaves of leaf color mutants; B: Anthocyanin content in fiber of leaf color mutant"

Fig. 6

GhCHS, GhLAR and GhANR expression level of leaves in leaf color mutants of G. hirsutum L."

Fig. 7

The expression level of GhCHS, GhLAR, GhANR in the fiber at different developmen stages in different leaf color mutants of G. hirsutum L."

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