Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (6): 1199-1217.doi: 10.3864/j.issn.0578-1752.2021.06.011

• HORTICULTURE • Previous Articles     Next Articles

Function Analysis of vvi-miR172s and Their Target Genes Response to Gibberellin Regulation of Grape Berry Development

XuXian XUAN1(),ZiLu SHENG1,ZhenQiang XIE2,YuQing HUANG1,PeiJie GONG1,Chuan ZHANG1,Ting ZHENG1,Chen WANG1(),JingGui FANG1   

  1. 1College of Horticulture, Nanjing Agricultural University, Nanjing 210095
    2Jiangsu Vocational College of Agriculture and Forestry, Jurong 212499, Jiangsu
  • Received:2020-06-01 Accepted:2020-11-19 Online:2021-03-16 Published:2021-03-25
  • Contact: Chen WANG E-mail:2019804199@njau.edu.cn;wangchen@njau.edu.cn

RichHTML

110

PDF

387

Abstract:

【Objective】 miR172 is an important regulator of plant growth and development. The purpose of this research was to clarify the roles and modes response to gibberellin (GA) of the miR172 family and its target genes in development of different tissues of grape berry. 【Method】The mature and precursor sequences of vvi-miR172a/b/c/d were cloned and identified by miR-RACE and PCR techniques from grapevine cv. Rosario Bianco. The target genes of vvi-miR172s were predicted by psRNA Target software, and the phylogenetic, structure analysis and subcellular localization were performed by bioinformatics tools. RLM-RACE verified the cleavage roles of four target genes by vvi-miR172s. The cis-elements analysis of their promoters was predicted by PLANTCARE software. The qRT-PCR method was used to detect the temporal and spatial expression patterns of vvi-miR172s and target genes in different tissues of grape berry induced by exogenous GA3 application. 【Result】The mature and precursor sequences of vvi-miR172a/b/c/d were cloned, and four target genes (VvAP2, VvRAP2-1, VvRAP2-2 and VvRAP2-3) were identified from grape genome. The cleavage sites of vvi-miR172s on target genes were detected by RLM-RACE, which proved that VvAP2, VvRAP2-1, VvRAP2-2 and VvRAP2-3 were the true target genes. Gene structure analysis result showed that all target genes contained 10 exons, 9 introns and 2 AP2 domains. The type and number of motif elements were similar, indicating that gene structures were highly conserved. Phylogenetic analysis showed that VvAP2, VvRAP2-1, and VvRAP2-2 were closer to poplar, and VvRAP2-3 had high homology with soybean. The secondary structure prediction of target proteins indicated that they existed in the form of alpha-helix and further folded into the stable tertiary structure. Subcellular localization results showed that all target proteins were mainly located in the nucleus. Both vvi-miR172c and VvRAP2-1 had the hormone related cis-elements response to GA3, suggesting that they might be involved in the regulation of grape berry development by responding to corresponding hormones. Microarray data analysis revealed that the expression patterns of vvi-miR172c and target genes were tissue or orGA3n specific. RT-qPCR analysis showed that vvi-miR172a/b/d showed a decrease expression trend in pericarp, while VvRAR2-1 exhibited a reverse expression trend to the former, indicating that vvi-miR172a/b/d neGA3tively regulated VvRAR2-1. However, in flesh, VvAP2 was contradictory to vvi-miR172d, with the increased expression during grape development, suggesting that there was a significant neGA3tive correlation between vvi-miR172d and VvAP2. Interestingly, GA3 treatment promoted the neGA3tive regulation of vvi-miR172d on VvRAP2-1 and induced the neGA3tive regulation of vvi-miR172c on VvAP2/VvRAP2-1/VvRAP2-3. 【Conclusion】VvAP2, VvRAP2-1, VvRAP2-2, and VvRAP2-3 were the real target genes of vvi-miR172s. Among the vvi-miR172 family, vvi-miR172 a/b/d might mediate VvRAP2-1 regulation of pericarp development, whereas vvi-miR172d might mediate VvAP2 involved in the development of grape flesh. vvi-miR172c/d and their target genes might participate in the regulation of grape pericarp and flesh development in response to exogenous GA.

Key words: grape, fruit development, gibberellin, miR172, target gene

Table 1

Sequence and use of primers"

引物名称 Primer name 引物序列 Primer sequence 引物用途 Primer use
vvi-miR172a-F AAGCTTTCGTAGTTGTCTACAAAATGATCTCT vvi-miR172a前体序列的扩增
Amplification of vvi-miR172a precursor
vvi-miR172a-R GGATCCCATCGCTTTACATTTTAGCCACT
vvi-miR172b-F AAGCTTCGGCATCTTTCTCCTCATACC vvi-miR172b前体序列的扩增
Amplification of vvi-miR172b precursor
vvi-miR172b-R GGATCCGGGTTGAAGACGTACATAAATCTCA
vvi-miR172c-F AAGCTTTTTGAAGCGTTCTGCTGTC vvi-miR172c前体序列的扩增
Amplification of vvi-miR172c precursor
vvi-miR172c-R GGATCCCAGATGAAACACTTCCAAGAGA
vvi-miR172d-F AAGCTTGAAGCGAGATCCGTATCCTC vvi-miR172d前体序列的扩增
Amplification of vvi-miR172d precursor
vvi-miR172d-F GGATCCATCTCAAATTACTGCATGATTTCAA
Actin-F TACAATTCCATCATGAAGTGTGATG Actin内参引物
Actin internal reference primer
Actin-R TTAGAAGCACTTCCTGTGAACAATG
VvAP2 qRT-F CTCGCGCATATGATAGGGCT VvAP2定量RT-PCR引物
Real-time PCR of VvAP2
VvAP2 qRT-R ACTTTGTCGGCGAAGGACAT
VvRAP2-1 qRT-F CAACACTGGGGGTTCATCCA VvRAP2-1定量RT-PCR引物
Real-time PCR of VvRAP2-1
VvRAP2-1 qRT-R TTTTGCCATGCCCAGTTTGG
VvRAP2-2 qRT-F CTGACTACCGTGCAAGGGTT VvRAP2-2定量RT-PCR引物
Real-time PCR of VvRAP-22
VvRAP2-2 qRT-R TTTGATCACCCTGGCCTAGC
VvRAP2-3 qRT-F GGGCTAGCAATGACATCGGA VvRAP2-3定量RT-PCR引物
Real-time PCR of VvRAP2-3
VvRAP2-3 qRT-R CATTTGCCACCCCCAGTTTG

Fig. 1

Growth and development of White Rosario grape under GA3 and CK treatments"

Fig. 2

Identification of vvi-miR172a/b/c/d precursor sequences and mature sequences alignment of miR172 in different species A: PCR products of vvi-miR172a/b/c/d primary sequences; 1: vvi-miR172a; 2: vvi-miR172b; 3: vvi-miR172c; 4: vvi-miR172d. B: Chromosomal location of vvi-miR172s and their targets genes; C: Hairpin structure of vvi-miR172s; D: Mature sequences alignment of miR172 in different species"

Table 2

Information of vvi-miR172s and target genes in grapevine"

miRNAs MiRNA成熟
体长度
Length of miRNA		 靶基因编号
Target_Acc.		 靶基因长度
Length of target		 靶区
Target regions		 靶区起始、
终止位点
Target start and stop sites		 错配率
Expectation		 允许不匹配
的最大能量
UPE		 切割方式
Interaction mode
vvi-miR172a/b 21 VIT_07s0031g00220(VvAP2) 2565 CDS:1870-1890 C、C 2.5 15.05 裂解Cleavage
21 VIT_08s0040g03180(VvRAP2-1) 1730 CDS:1331-1351 C、T 2.5 11.489 裂解Cleavage
21 VIT_13s0019g03550(VvRAP2-2) 2217 CDS:1566-1586 C、C 2.5 17.172 裂解Cleavage
21 VIT_06s0004g03590(VvRAP2-3) 2347 CDS:1806-1826 C、T 2.5 14.04 裂解Cleavage
vvi-miR172c 21 VIT_07s0031g00220(VvAP2) 2565 CDS:1870-1890 C、C 0.5 15.05 裂解Cleavage
21 VIT_08s0040g03180(VvRAP2-1) 1730 CDS:1331-1351 C、T 1.0 11.489 裂解Cleavage
21 VIT_13s0019g03550(VvRAP2-2) 2217 CDS:1566-1586 C、C 0.5 17.172 裂解Cleavage
21 VIT_06s0004g03590(VvRAP2-3) 2347 CDS:1806-1826 C、T 1.0 14.04 裂解Cleavage
vvi-miR172d 23 VIT_07s0031g00220(VvAP2) 2565 CDS:1870-1892 C、A 2.0 15.552 裂解Cleavage
23 VIT_08s0040g03180(VvRAP2-1) 1730 CDS:1331-1353 C、G 1.5 9.197 裂解Cleavage
23 VIT_13s0019g03550(VvRAP2-2) 2217 CSS:1566-1588 G、A 2.5 22.9 裂解Cleavage
23 VIT_06s0004g03590(VvRAP2-3) 2347 CDS:1806-1828 C、A 0.5 14.075 裂解Cleavage

Fig. 3

Match degree of vvi-miR172s and their target genes"

Fig. 4

The cleavage site between vvi-miR172s and target genes The dotted lines represent the cleavage sites; numbers indicate the frequency of clones at 5 termini of mRNA fragments"

Fig. 5

Phylogenetic relationship and gene structure and conserved motifs analysis of target genes A: Phylogeny tree of target genes; B: Gene structure of target genes; C: The conserved motifs of target genes"

Fig. 6

Structure analysis and subcellular localization prediction of target proteins A: The secondary structure prediction of target proteins; B: The tertiary structure prediction of target proteins; C: The conserved domains prediction of target proteins; D: The subcellular localization prediction of target proteins"

Fig. 7

Predicted cis-elements in the promoter of vvi-miR172s and their target genes A: The total number of diverse types of cis-elements derived from vvi-miR172s and their target genes promoters; B: Hormone-related cis-elements of vvi-miR172s and their target genes"

Fig. 8

Expression levels of vvi-miR172s (GEO:GSE59802) and target genes (GEO:GSE36128) in different orGA3ns, tissues and development stages A: Expression profiles of vvi-miR172c and target genes in different orGA3ns, tissures and development satges; B: Expression of vvi-miR172c and target genes in different tissues. Bud-AB: Bud after brust; Inflorescence-WD: Well development inflorescence; Flower-FB: Flowering begins; Flower-F: Flowering; Rachis-PFS: Rachis post fruit set; Stem-G: Green stem; Stem-W: Woody stem; Seed-G: Seed collected from green berries"

Fig. 9

Expression levels of vvi-miR172a/b/c/d and its target genes in berry pericarp (A) and flesh (B) of grape *,** represented significant difference (P<0.05) and extremely significant difference (P<0.01), respectively. The same as below"

Fig. 10

Expression levels of vvi-miR172a/b/c/d and target genes in berry pericarp (A) and flesh (B) of grape under GA3 treatment"

Fig. 11

Differential expression and correlation analysis of each member of vvi-miR172 family and target genes in response to GA3 treatment in berry pericarp (A) and flesh (B) of grape"

[1] WANG T Y, PING X K, CAO Y R, JIAN H J, GA3O Y M, WANG J, TAN Y C, XU X F, LU K, LI J N, LIU L Z. Genome-wide exploration and characterization of miR172/euAP2 genes in Brassica napus L. for likely role in flower organ development. BMC Plant Biology, 2019,19(1):336.
[2] XU Z S, CHEN M, LI L C, MA Y Z. Functions and application of the AP2/ERF transcription factor family in crop improvement. Journal of Integrative Plant Biology, 2011,53(7):570-585.
doi: 10.1111/j.1744-7909.2011.01062.x pmid: 21676172
[3] LI J, LUAN Y S, ZHAI J M, LIU P, XIA X Y. Bioinformatic analysis of functional characteristics of miR172 family in tomato. Journal of Northeast Agricultural University (English edition), 2013,20(4):21-29.
[4] 王梦琦, 解振强, 孙欣, 李晓鹏, 朱旭东, 王晨, 房经贵. 葡萄miR159及其靶基因VvGAMYB在花发育过程中的作用分析. 园艺学报, 2017,44(6):1061-1072.
WANG M Q, XIE Z Q, SUN X, LI X P, ZHU X D, WANG C, FANG J G. Function analysis of miR159 and its target gene VvGAMYB in grape flower development. Acta Horticulturae Sinica, 2017,44(6):1061-1072. (in Chinese)
[5] MATHIEU J, YANT L J, MURDTER F, KUTTNER F, SCHMID M. Repression of flowering by the miR172 target SMZ. PLoS Biology, 2009,7(7):e1000148.
doi: 10.1371/journal.pbio.1000148 pmid: 19582143
[6] ZENG C Y, WANG W Q, ZHENG Y, CHEN X, BO W P, SONG S, ZHANG W X, PENG M. Conservation and divergence of microRNAs and their functions in Euphorbiaceous plants. Nucleic Acids Research, 2010,38(3):981-995.
doi: 10.1093/nar/gkp1035 pmid: 19942686
[7] TANG M Y, BAI X, NIU L J, CHAI X, CHEN M S, XU Z F. miR172 regulates both vegetative and reproductive development in the perennial woody plant Jatropha curcas. Plant and Cell Physiology, 2018,59(12):2549-2563.
doi: 10.1093/pcp/pcy175 pmid: 30541045
[8] ZHU Q H, HELLIWELL C A. Regulation of flowering time and floral patterning by miR172. Journal of Experimental Botany, 2011,62:487-495.
[9] 侍婷, 高志红, 章镇, 庄维兵. MicroRNA参与植物花发育调控的研究进展. 中国农学通报, 2010,26(13):267-271.
SHI T, GAO Z H, ZHANG Z, ZHUANG W B. Advance of research on microRNA in flower development regulation. Chinese Agricultural Science Bulletin, 2010,26(13):267-271. (in Chinese)
[10] YAO J L, TOMES S, XU J, GLEAVE A P. How microRNA172 affects fruit growth in different species is dependent on fruit type. Plant Signaling and Behavior, 2016,11(4):e1156833.
doi: 10.1080/15592324.2016.1156833 pmid: 26926448
[11] RIPOLL J J, BAILRY L J, MAI Q A, WU S L, HON C T, CHAPMAN E J, DITTA G S, YANOFSKY M E, YANOFSKY M F. microRNA regulation of fruit growth. Nature Plants, 2015,1(4):15036.
doi: 10.1038/nplants.2015.36 pmid: 27247036
[12] CHEN X M. A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development. Science, 2004,303(5666):2022-2025.
doi: 10.1126/science.1088060 pmid: 12893888
[13] JUNG J H, SEO Y H, SEO P J, REYES J L, JU Y, CHUA N H, PARK C M. The GIGANTEA-regulated microRNA172 mediates photoperiodic flowering independent of CONSTANS in Arabidopsis. Plant Cell, 2007,19(9):2736-2748.
doi: 10.1105/tpc.107.054528 pmid: 17890372
[14] JUNG J H, SEO P J, KANG S K, PARK C M. MiR172 signals are incorporated into the miR156 signaling pathway at the SPL3/4/5 genes in Arabidopsis developmental transitions. Plant Molecular Biology, 2011,76(1):35-45.
[15] YANT L, MATHIEU J, DINH T T, OTT F, LANZ C, WOLLMANN H, CHEN X, SCHMID M. Orchestration of the floral transition and floral development in Arabidopsis by the bifunctional transcription factor APETALA2. Plant Cell, 2010,22(7):2156-2170.
doi: 10.1105/tpc.110.075606 pmid: 20675573
[16] BARTLEY G E, ISHIDA B K. Digital fruit ripening: Data mining in the TIGR tomato gene index. Plant Molecular Biology Reporter, 2002,20(2):115-130.
[17] ALBA R, PAYTON P, FEI Z J, MCQUINN R, DEBBIE P, MARTIN G B, TANKSLEY S D, GIOVANNONI J J. Transcriptome and selected metabolite analyses reveal multiple points of ethylene control during tomato fruit development. Plant Cell, 2005,17(11):2954-2965.
doi: 10.1105/tpc.105.036053 pmid: 16243903
[18] CHUNG M Y, VREBALOV J, ALBA R, LEE J, MCQUINN R, CHUNG J D, KLEIN P, GIOVANNONI J. A tomato (Solanum lycopersicum) APETALA2/ERF gene, SlAP2a, is a negative regulator of fruit ripening. Plant Journal, 2010,64(6):936-947.
[19] 滕飞. 拟南芥AP2/ERF基因ERF055调控茎端分生组织发育的功能研究[D]. 泰安: 山东农业大学, 2013.
TENG F. Functional study of Arabidopsis AP2/ERF family gene ERF055 in regulation of the development of the shoot apical meristem[D]. Tai’an: Shangdong Agrilcultural University, 2013. (in Chinese)
[20] 张计育, 王庆菊, 郭忠仁. 植物AP2/ERF类转录因子研究进展. 遗传, 2012,34(7):835-847.
ZHANG J Y, WANG Q J, GUO Z R. Progresses on plant AP2/ERF transcription factors. Hereditas, 2012,34(7):835-847. (in Chinese)
[21] 王文然, 王晨, 解振强, 贾海锋, 汤崴, 崔梦杰, 房经贵. VvmiR397a及其靶基因VvLACs在葡萄果实发育中的作用分析. 园艺学报, 2018,45(8):1441-1455.
WANG W R, WANG C, XIE Z Q, JIA H F, TANG W, CUI M J, FANG J G. Function analysis of VvmiR397a and its target genes VvLACs in grape berry development. Acta Horticulturae Sinica, 2018,45(8):1441-1455. (in Chinese)
[22] KUNST L, KLENZ J E, MARTINEZ-ZAPATER J, HAUGHN G W. AP2 gene determines the identity of perianth organs in flowers of Arabidopsis thaliana. Plant Cell, 1989,1(12):1195-1208.
doi: 10.1105/tpc.1.12.1195 pmid: 12359889
[23] OKAMURO J K, BOER BGW, JOFUKU KD. Regulation of Arabidopsis flower development. Plant Cell, 1993,5(10):1183-1193.
pmid: 8281037
[24] NIU X, HELENTJARIS T, BATE N J. Maize ABI4 binds coupling element1 in abscisic acid and sugar response genes. Plant Cell, 2002,14(10):2565-2575.
pmid: 12368505
[25] WANG C, ShANGGUAN L F, NICHOLAS K K, WANG X C, HAN J, SONG C N, FANG J G. Characterization of microRNAs identified in a table grapevine cultivar with validation of computationally predicated grapevine miRNAs by miR-RACE. PLoS ONE, 2016(7):e21259.
doi: 10.1371/journal.pone.0021259 pmid: 21829435
[26] 张文颖, 王晨, 朱旭东, 马超, 王文然, 冷祥鹏, 郑婷, 房经贵. 葡萄全基因组DELLA蛋白基因家族鉴定及其应答外源赤霉素调控葡萄果实发育的特征. 中国农业科学, 2018,51(16):3130-3146.
ZHANG W Y, WANG C, ZHU X D, MA C, WANG W R, LENG X P, ZHENG T, FANG J G. Genome-wide identification and expression of DELLA protein gene family during the development of grape berry induced by exogenous GA. Scientia Agricultura Sinica, 2018,51(16):3130-3146. (in Chinese)
[27] 朱旭东. 葡萄蔗糖合酶基因VvSS3的初步功能研究[D]. 南京: 南京农业大学, 2017.
ZHU X D. The preliminary function analysis of the sucrose synthase gene VvSS3 in Vitis vinifera[D]. Nanjing: Nanjing Agricultural University, 2017. (in Chinese)
[28] AKULLAN J B, PINTO D L P, BERTOLINI E, FASOLI M, ZENONI S, TORNIELLI G B, PEZZOTTI M, MEYERS B C, FARINA L, PE M E, MICA E. miRVine: A microRNA expression atlas of grapevine based on small RNA sequencing. BMC Genomics, 2015,16:393.
pmid: 25981679
[29] NAKANO T, SUZUKI K, FUJIMURA T, SHINSHI H. Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiology, 2006,140(2):411-432.
[30] TANG M F, LI G S, CHEN M S. The phylogeny and expression pattern of APETALA2-like genes in rice. Journal of Genetics and Genomics, 2007,34(10):930-938.
pmid: 17945171
[31] GIL-HUMANES J, PISTON F, MARTIN A, BARRO F. Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids. BMC Plant Biology, 2009,9(1):66-79.
[32] ZHUANG J, CAI B, PENG R H, ZHU B, JIN X F, XUE Y, GAO F, FU X Y, TIAN Y S, ZHAO W, QIAO Y S, ZHANG Z, XIONG A S, YAO Q H. Genome-wide analysis of the AP2/ERF gene family in Populus trichocarpa. Biochemical and Biophysical Research Communications, 2008,371(3):468-474.
doi: 10.1016/j.bbrc.2008.04.087 pmid: 18442469
[33] ZHANG G Y, CHEN M, CHEN X P, XU Z S, GUAN S, LI L C, LI A L, GUO J M, MAO L, MA Y Z. Phylogeny, gene structures, and expression patterns of the ERF gene family in soybean (Glycine max L.). Journal of Experimental Botany, 2008,59(15):4095-4107.
pmid: 18832187
[34] LI J P, CHEN F J, LI Y Q, LI P C, WANG Y Q, MI G H, YUAN L X. ZmRAP2.7, an AP2 transcription factor, is involved in Maize brace roots development. Frontiers in plant science, 2019,10:820.
doi: 10.3389/fpls.2019.00820 pmid: 31333689
[35] JOFUKU K D, DEN BOER B D, VAN MONTAGU M, OKAMURO J K. Control of Arabidopsis flower and seed development by the homeotic gene APETALA2. Plant Cell, 1994,6(9):1211-1225.
doi: 10.1105/tpc.6.9.1211 pmid: 7919989
[36] ZHU Q H, HELLIWELLl C A. Regulation of flowering time and floral patterning by miR172. Journal of Experimental Botany, 2011,62(2):487-495.
doi: 10.1093/jxb/erq295 pmid: 20952628
[1] ZHANG KeKun,CHEN KeQin,LI WanPing,QIAO HaoRong,ZHANG JunXia,LIU FengZhi,FANG YuLin,WANG HaiBo. Effects of Irrigation Amount on Berry Development and Aroma Components Accumulation of Shine Muscat Grape in Root-Restricted Cultivation [J]. Scientia Agricultura Sinica, 2023, 56(1): 129-143.
[2] LÜ XinNing,WANG Yue,JIA RunPu,WANG ShengNan,YAO YuXin. Effects of Melatonin Treatment on Quality of Stored Shine Muscat Grapes Under Different Storage Temperatures [J]. Scientia Agricultura Sinica, 2022, 55(7): 1411-1422.
[3] GUO ZeXi,SUN DaYun,QU JunJie,PAN FengYing,LIU LuLu,YIN Ling. The Role of Chalcone Synthase Gene in Grape Resistance to Gray Mold and Downy Mildew [J]. Scientia Agricultura Sinica, 2022, 55(6): 1139-1148.
[4] LI ShiJia,LÜ ZiJing,ZHAO Jin. Identification of R2R3-MYB Subfamily in Chinese Jujube and Their Expression Pattern During the Fruit Development [J]. Scientia Agricultura Sinica, 2022, 55(6): 1199-1212.
[5] WANG HuiLing, YAN AiLing, SUN Lei, ZHANG GuoJun, WANG XiaoYue, REN JianCheng, XU HaiYing. eQTL Analysis of Key Monoterpene Biosynthesis Genes in Table Grape [J]. Scientia Agricultura Sinica, 2022, 55(5): 977-990.
[6] XIE LingLi,WEI DingYi,ZHANG ZiShuang,XU JinSong,ZHANG XueKun,XU BenBo. Dynamic Changes of Gibberellin Content During the Development and Its Relationship with Yield of Brassica napus L. [J]. Scientia Agricultura Sinica, 2022, 55(24): 4793-4807.
[7] WANG Bo,QIN FuQiang,DENG FengYing,LUO HuiGe,CHEN XiangFei,CHENG Guo,BAI Yang,HUANG XiaoYun,HAN JiaYu,CAO XiongJun,BAI XianJin. Difference in Flavonoid Composition and Content Between Summer and Winter Grape Berries of Shine Muscat Under Two-Crop-a-Year Cultivation [J]. Scientia Agricultura Sinica, 2022, 55(22): 4473-4486.
[8] WANG ShuaiYu,ZHANG ZiTeng,XIE AiTing,DONG Jie,YANG JianGuo,ZHANG AiHuan. Mutation Analysis of Insecticide Target Genes in Populations of Spodoptera frugiperda in China [J]. Scientia Agricultura Sinica, 2022, 55(20): 3948-3959.
[9] LIU Xin,ZHANG YaHong,YUAN Miao,DANG ShiZhuo,ZHOU Juan. Transcriptome Analysis During Flower Bud Differentiation of Red Globe Grape [J]. Scientia Agricultura Sinica, 2022, 55(20): 4020-4035.
[10] MA YuQuan,WANG XiaoLong,LI YuMei,WANG XiaoDi,LIU FengZhi,WANG HaiBo. Differences in Nutrient Absorption and Utilization of 87-1 Grape Variety Under Different Rootstock Facilities [J]. Scientia Agricultura Sinica, 2022, 55(19): 3822-3830.
[11] JI XiaoHao,LIU FengZhi,WANG BaoLiang,LIU PeiPei,WANG HaiBo. Genetic Variation of Alcohol Acyltransferase Encoding Gene in Grape [J]. Scientia Agricultura Sinica, 2022, 55(14): 2797-2811.
[12] YANG ShengDi,MENG XiangXuan,GUO DaLong,PEI MaoSong,LIU HaiNan,WEI TongLu,YU YiHe. Co-Expression Network and Transcriptional Regulation Analysis of Sulfur Dioxide-Induced Postharvest Abscission of Kyoho Grape [J]. Scientia Agricultura Sinica, 2022, 55(11): 2214-2226.
[13] HAN Xiao, YANG HangYu, CHEN WeiKai, WANG Jun, HE Fei. Effects of Different Rootstocks on Flavonoids of Vitis vinifera L. cv. Tannat Grape Fruits [J]. Scientia Agricultura Sinica, 2022, 55(10): 2013-2025.
[14] XU XianBin,GENG XiaoYue,LI Hui,SUN LiJuan,ZHENG Huan,TAO JianMin. Transcriptome Analysis of Genes Involved in ABA-Induced Anthocyanin Accumulation in Grape [J]. Scientia Agricultura Sinica, 2022, 55(1): 134-151.
[15] LIU Chuang,GAO Zhen,YAO YuXin,DU YuanPeng. Functional Identification of Grape Potassium Ion Transporter VviHKT1;7 Under Salt Stress [J]. Scientia Agricultura Sinica, 2021, 54(9): 1952-1963.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd