Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (07): 1364-1368 .

• PLANT PROTECTION • Previous Articles     Next Articles

Molecular Markers of Wheat Leaf Rust Resistance Gene Lr45 Based on AFLP

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  1. 河北农业大学植物病理系
  • Received:2004-12-01 Revised:1900-01-01 Online:2005-07-10 Published:2005-07-10

Abstract: AFLP technology was employed to isolate wheat leaf rust resistance gene Lr45. Sixty primer pairs were screened, of which two primer pairs, P-AGG/M-GAG and P-ACA/M-GGT, displayed polymorphsim in the population of TcLr45, Thatcher, and their F2 generation. Two markers were found tightly linked to Lr45. One links to the Lr45 locus with a distance of 0.6 cM, and the other with a distance of 1.3 cM. The fragement of 261 bp was 86 percent the same as a part of the original sequence of Vulgare HortI gene, and the 105 bp was 96 percent compared with a part of the triticum monococcum phosphatidylserine decarboxylase gene. Both of them included an open reading frame (ORF).

Key words: Wheat leaf rust, AFLP, Lr45, Gene localization

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