Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (16): 3273-2380.doi: 10.3864/j.issn.0578-1752.2012.16.006

• PLANT PROTECTION • Previous Articles     Next Articles

Genetic Analysis and Molecular Mapping of Leaf Rust Resistance Genes in Two Chinese Wheat Lines

 ZHOU  Yue, WU  Yu, LI  Xing, LI  Zai-Feng, LIU  Da-Qun   

  1. 河北农业大学植物保护学院/河北省农作物病虫害生物防治工程技术研究中心，河北保定 071001
  • Received:2011-11-07 Online:2012-08-15 Published:2012-02-21

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Abstract: 【Objective】 The objective of this study is to identify the leaf rust resistance genes of two Chinese wheat (Triticum aestivum L.) lines SW8588 and Mianyang 351-15 from Sichuan province, and to provide a basis for breeding wheat cultivars with durable resistance. 【Method】 Mianyang 351-15, SW8588 and 30 differential lines with known leaf rust resistance genes were inoculated with 15 pathotypes of Puccinia triticina for postulating leaf rust resistance genes in the two lines. The two F1 and F2 populations from crosses of Mianyang 351-15/Zhengzhou 5389 and SW8588/Zhengzhou 5389, were inoculated with pathotype FHTT in the greenhouse. The infection types were scored 15 days after inoculation and molecular markers were also used for mapping the resistance genes.【Result】SW8588 did not contain the known leaf rust resistance gene Lr1 and Mianyang 351-15 maybe contain Lr1 and other unknown genes. When inoculating with pathotype FHTT, only one resistance gene was detected in each of the two resistance lines. Using SSR makers, the resistance gene in Mianyang 351-15 was mapped on 5DL and linked to SSR markers barc144 and wmc765 with genetic distances of 8.9 and 20.8 cM, respectively. The STS marker WR003 for Lr1 was co-segregated with this resistance gene in Mianyang 351-15. Based on these results, it was concluded that Mianyang 351-15 contained Lr1 conferring the resistance to FHTT. Using genetic analysis and molecular markers it could be found that SW8588 carried a single dominant resistance gene, temporarily designated LrSW85, co-segregating with STS marker WR003, which may be an allele of Lr1 or was closely linked to Lr1.【Conclusion】Based on gene postulation, genetic analysis and molecular mapping it can be concluded that Mianyang 351-15 contained the leaf rust resistance gene Lr1 and SW8588 contained the leaf rust resistance gene LrSW85 which may be an allele of Lr1 or closely linked to Lr1.

Key words: wheat leaf rust resistance gene, gene mapping, SSR marker, STS marker

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