Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (23): 5058-5065.doi: 10.3864/j.issn.0578-1752.2013.23.024

• RESEARCH NOTES • Previous Articles     Next Articles

cDNA-AFLP Analysis of Differentially Expressed Resistant Genes of Minn2761

 LUAN  Zhao-Jie, CAO  Yuan-Yin, LI  Tian-Ya, CHEN  Si, CHEN  Xiu-Mei, ZHU  Gui-Qing, LI  Wei-Hua   

  1. College of Plant Protection, Shenyang Agricultural University, Shenyang 110866
  • Received:2013-05-06 Online:2013-12-01 Published:2013-07-10

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Abstract: 【Objective】The objective of this study is to analyze the differential genes expression of the important Chinese supplementary differential Minn2761 induced by wheat stem rust, and to find out the fragments related to disease-resistant gene expression. 【Method】 The study was carried out by cDNA-AFLP analysis of differentially expressed resistant genes in Minn2761 and Thatcher inoculated with wheat stem rust.【Result】About 7 000 bands were amplified by 140 primer combinations and by average 50 bands appeared per pair of primers. Through cDNA-AFLP analysis, 86 primer combinations amplified specific bands between Minn2761 and Thatcher. Thirty-five possibly disease resistance-related transcript derived fragments (TDFs) were cloned and sequenced. BLASTx analysis showed that the sequences of 30 TDFs were found to be homologous with those in GenBank. Among them, 24 TDFs had known function and 6 TDFs had unknown function.【Conclusion】Eight TDFs having a highly homology with known genes which encoded kinase binding protein, NBS-LRR resistance protein, cinnamoyl CoA reductase-like 2b, cinnamoyl CoA reductase-like 2c, sucrose-phosphate synthase 7, metacaspase-1-like isoform 1, Zn-finger-like protein, and putative disease resistance protein RGA4-like were supposed to the resistance to stem rust of Minn2761.

Key words: wheat stem rust , Minn2761 , cDNA-AFLP , resistance gene

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