Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (19): 4119-4130.doi: 10.3864/j.issn.0578-1752.2013.19.018

• HORTICULTURE • Previous Articles     Next Articles

Differential Genes Expression in Tea Plant (Cameilla sinensis L.) Induced by Ectropis oblique Feeding Based on cDNA-AFLP

 CAO  Shi-Xian, CHENG  Xi, JIANG  Zheng-Zhong, SHENG  Liang, SHANG  Guan-Ming-Zhu, DENG  Wei-Wei, WEI  Chao-Ling   

  1. College of Tea & Food Science and Technology, Anhui Agricultural University/Ministry-State Key Laboratory Breeding Base of Tea Biochemistry & Biotechnology/Key Laboratory of Tea Plant Biology & Tea Processing, Ministry of Agriculture, Hefei 230036
  • Received:2013-04-15 Online:2013-10-01 Published:2013-06-04

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Abstract: 【Objective】 The objective of this study is to screen the defense genes in tea plant (Camellia sinensis L.) induced by tea looper (Ectropis oblique).【Method】Tea leaves fed by the looper were analyzed by cDNA-AFLP(cDNA-amplified fragment length polymorphism). Transcript-derived fragments (TDF) were screened and confirmed. After that, bioinformatics analysis was followed. And the expression patterns of the differential genes were performed by qRT-PCR.【Result】The results showed that 134 EST(expressed sequence tags) were obtained by sequencing and identifying from the selected 231 TDFs. Among them, 81 TDFs were up-regulated, and 53 were down-regulated, which accounted for 60.4% and 39.6% of the obtained fragments, respectively. According to BLASTx result of TDFs, a large group (23.1%) of these genes shared high homology with genes involved in metabolism, 9.0% genes with functions related to photosynthesis and energy, 8.2% genes with functions related to protein synthesis and storage, signal transduction, 5.2% genes with functions related to disease/defense. But only few genes involved in cell growth and structure, transcription factor and transporters and the ratios were 1.5%, 2.2%, and 3.0%, respectively. Moreover, sequences with unknown proteins occupied 6.7%; and 20.1% sequences with no similarity against the database. TDF-21F1, TDF-22D, TDF-27L3 and TDF-28F were confirmed by qRT-PCR analysis, further result indicated that the expression of the defense genes responsive to Ectropis oblique play significant regulation roles in adapting to negative environment for tea plant.【Conclusion】Using cDNA-AFLP method to analyze Ectropis oblique feeding tea plant and get the TDFs, it could supply valuable information to the study of the molecular mechanism between tea and pest.

Key words: Camellia sinensis , Ectropis oblique , feeding-induced , cDNA-AFLP , defense mechanism

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