Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (2): 353-358.doi: 10.3864/j.issn.0578-1752.2012.02.018

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

cDNA-AFLP Analysis on Transcripts Associated Gene with Broodiness in Muscovy Duck

 WU  Xu, YAN  Mei-Jiao, LIU  Li-Ping, FU  Xing-Yuan, LIAN  Sen-Yang, LI  Ang   

  1. 1.福建农林大学动物科学学院，福州 350002
  • Received:2011-03-04 Online:2012-01-15 Published:2011-07-19

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Abstract: 【Objective】 The objective of this experiment is to identify differentially expressed genes of Muscovy Duck broodiness. 【Method】cDNA-AFLP approach was employed to analyze genes differentially expressed in broodiness and non-broodiness Muscovy Duck individuals derived from a single RF white feathers line. 【Result】 There were 82 differentially expressed bands associated with broodiness behaviors, 15 bands of them were subject to sequence analysis. Database searches indicate that 8 cDNA fragments show high homology to known genes, which include some function genes in hypothalamic- pituitary-gonadal axis regulating reproduction, such as GnRH gene and FSH gene. Meanwhile genes were also found in GH-IGF axis, such as GH gene. Other genes participated in signal transduction, transcription, metabolism biological processes, such as superoxide dismutase, keratin related protein, thiocyanate enzyme release S-transferase enzymes etc. Two cDNA fragments show homology to unknown function genes, 5 cDNA fragments show no detectable homology to know genes, suggesting that they probably represent novel genes. Three bands selected randomly from them were confirmed by RT-PCR and that result was consistent with the results of cDNA-AFLP. 【Conclusion】 The results of this paper uncovered the regulation mode of gene express variation of different Muscovy Duck broodiness traits, and thus further providing the molecular mechanism of broodiness of Muscovy Duck.

Key words: Muscovy Duck (Cairina moschata), broodiness, cDNA-AFLP, gene expression profiling, differentially expressed genes

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