Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (23): 5003-5011.doi: 10.3864/j.issn.0578-1752.2013.23.017

• HORTICULTURE • Previous Articles     Next Articles

Analysis of the Genetic Relationship of the Main Cultivars of Ping’ou Hybrid Hazelnut (C. heterophylla×C. avellana) by FISH-AFLP Markers

 MA  Qing-Hua-1, CHEN  Xin-2, ZHAO  Tian-Tian-1, LIU  Qing-Zhong-2, WANG  Gui-Xi-1   

  1. 1、Research Institute of Forestry, Chinese Academy of Forestry/Key Laboratory of Forestry Silviculture of State Forestry Administration/State Key Laboratory of Tree Genetics and Breeding, Beijing 100091;
    2、Shandong Institute of Pomology/Shandong Provincial Key Laboratory of Fruit Tree Biotechnology Breeding, Tai’an 271000, Shandong
  • Received:2013-05-28 Online:2013-12-01 Published:2013-08-21

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Abstract: 【Objective】The objective of this study is to establish FISH-AFLP analysis system for Ping’ou hybrid hazelnut and analyze the genetic relationship of the main cultivars.【Method】Ten main cultivars, such as Dawei, were selected as materials. High-quality genomic DNA was extracted with improved CTAB method, and the purified DNA samples were digested with PstⅠ/MseⅠ. After ligation reaction, the samples were used to perform FISH-AFLP detection. The information of all bands was converted into “0-1” matrix. NTSYS pc 2.11F and Popgene 1.32 software were used to conduct the data analysis and plotting.【Result】The results showed that: 15 PstⅠ/MseⅠ primer pairs were selected from 64 combinations with high polymorphism, strong signals and clear bands. A total of 1 739 bands were obtained. The average percentage of polymorphic bands was 97.94%. The similarity coefficient of the main cultivars varied from 0.7556 to 0.8543. All the samples were divided into 4 groups when the threshold value was 0.8398. Each in cutivars of Yuzhui (84-310), Liaozhen No. 4 (85-41) and Ping’ou 69 (84-69) formed an independent group alone and the others constituted the fourth group. The effective number of alleles, Nei’s gene diversity and Shannon’s information index of the main cultivars were 1.3921, 0.2482 and 0.3957, respectively, indicating high genetic diversity among Ping’ou hybrid hazelnut. The unique bands obtained in the experiments could be used in the rapid identification of the main cultivars of Ping’ou hybrid hazelnut. 【Conclusion】 Due to the complex selecting group, Ping’ou hybrid hazelnut is a high polymorphic population and the genetic relationships of the main cultivars are complex. The FISH-AFLP analysis system, PstⅠ/MseⅠprimer pairs and all the genetic parameters obtained in this research can offer reference for the relative researches in Corylus.

Key words: Ping’ou hybrid hazelnut (C. heterophylla×C. avellana) , FISH-AFLP markers , fingerprinting , genetic relationship , genetic diversity

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