两种微生物菌剂对有机基质袋培秋黄瓜产量、品质及根际环境的影响

doi:10.3864/j.issn.0578-1752.2021.14.013

Abstract

Abstract:

【Objective】A bag-cultivated experiment was conducted to test the potential of two microbial agents (Bacillus methylotrophicus and Lactobacillus plantarum) on the yield, quality and rhizosphere environment of autumn cucumber using organic substrate as a growth medium. The results of the experiment were helpful to develop the new functional type of microbial agents to guide the high quality and high efficiency production of cucumber. 【Method】Cucumber cultivar ‘Bonai 526’ was sown in a bag of organic substrate. There were a total of six treatments, including CK1 (Irrigation of water and non-application of microbial agents), NT1 (Irrigation of water and application of Bacillus methylotrophicusagents), NT2 (Irrigation of water and application of Lactobacillus plantarumagents), CK2 (Irrigation of nutrient solution and non-application of microbial agents), FT1 (Irrigation of nutrient solution and application ofBacillus methylotrophicusagents), and FT2 (Irrigation of nutrient solution and application of Lactobacillus plantarumagents). The microbial agents (Bacillus methylotrophicus as ‘VL-10’ and Lactobacillus plantarum as ‘LYS-1’) were added to the cucumber rhizosphere at the rate of 2.5×10¹⁰ CFU/plant on 20th, 35th and 50th day. 【Result】Compared with CK1 and CK2 treatment,Bacillus methylotrophicus inoculation in both of the nutrient levels increased the dry matter content by 13.51% and 15.02%, and yield by 20.83% and 15.63%, respectively; Lactobacillus plantarum increased the dry matter content by 11.43% and 8.42%, and the yield by 17.42% and 14.96%, respectively. The fruit quality under FT1 treatment was the best; compared with CK2 treatment, its content of free amino acid, organic acid, soluble sugar, reducing sugar and vitamin C were significantly increased by 10.61%, 28.93%, 22.92% and 39.88%, respectively,, followed by FT2 treatment. The accumulation of phosphorus and potassium were significantly higher under both NT1 (7.43% and 10.60%) and NT2 (13.50% and 8.19%) compared with CK1 treatment (P<0.05). Compared with CK2 treatment, the accumulation of nitrogen, and potassium were significantly higher under FT2 (24.18% and 26.25%), while the phosphorus concentration in plants were significantly higher under FT1 (17.16%). Compared with CK2 treatment, the nitrogen, phosphorus and potassium fertilizer utilization rates under FT1 treatment were significantly increased by 82.85%, 483.90% and 75.60% (P<0.05), respectively, which under FT2 treatment was significantly increased by 102.42%, 367.98% and 120.46%, respectively (P<0.05). Compared with CK1 treatment, the treatments NT1 and NT2 improved soil enzymatic activities of sucrase (100.66% and 116.60%), catalase (3.39% and 4.10%) and alkaline phosphatase (6.99% and 95.08%) at the full fruiting stage, among which catalase and alkaline phosphatase activities were still higher at the end of the experiment. Compared with CK2 treatment, the FT1 treatment significantly improved soil enzymatic activities of urease (3.75% and 13.13%), sucrase (68.62% and 31.68%) and alkaline phosphatase (18.00% and 109.64%) in the full fruiting period and the seedling pulling period, while the urease (4.95% and 6.12 %), sucrase (24.93% and 63.35%) and alkaline phosphatase activity (26.99% and 84.01%) under FT2 treatment (P<0.05). The effect ofBacillus methylotrophicagent on the urease and alkaline phosphatase activities were better than that ofLactobacillus plantarumagent. Moreover, the substrate incubated with Bacillus methylotrophic showed significantly higher available nitrogen content (63.33% and 72.70%) (P<0.05) compared with their respective controls at the full fruiting stage, and the increase was 25.48% and 86.46% at the end of the experiment, respectively. 【Conclusion】In conclusion, adding 7.5×10¹⁰CFU/plant of Bacillus methylotrophic to the substrate could improve the rhizosphere environment of cucumber and promote the absorption, assimilation and accumulation of essential elements by the plant. Moreover, it could effectively improve the yield and fruit quality of cucumber.

Key words: microbial agents, cucumber, substrate cultivation, yield, fruit quality, rhizosphere environment

WANG JunZheng,ZHANG Qi,GAO ZiXing,MA XueQiang,QU Feng,HU XiaoHui. Effects of Two Microbial Agents on Yield, Quality and Rhizosphere Environment of Autumn Cucumber Cultured in Organic Substrate[J].Scientia Agricultura Sinica, 2021, 54(14): 3077-3087.

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References 37

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处理 Treatment		游离氨基酸 Free amino acids (μg·g^-1)	可溶性蛋白 Soluble protein (μg·g^-1)	有机酸 Organic acid (%)	可溶性糖 Soluble sugar (%)	还原糖 Reducing sugar (%)	维生素C Vitamin C (μg·g^-1)	硝酸盐 Nitrate content (μg·g^-1)
CK1		609.60±6.13f	296.30±5.86c	1.22±0.013d	8.17±0.17d	1.12±0.004c	55.14±0.58d	273.07±4.72d
NT1		700.93±9.03d	349.11±1.08b	1.29±0.017c	9.26±0.34c	1.43±0.068b	55.14±1.01d	338.89±5.16b
NT2		648.57±6.71e	238.72±4.47d	1.42±0.009b	9.12±0.17c	1.37±0.048b	63.88±1.21cd	309.30±5.16c
CK2		745.72±9.07c	373.36±2.81a	1.44±0.013b	9.54±0.23c	1.44±0.080b	73.29±0.58bc	415.58±12.55a
FT1		824.84±7.63a	387.21±4.22a	1.53±0.004a	12.30±0.38a	1.77±0.078a	102.52±5.61a	359.42±5.23b
FT2		800.41±6.48b	302.36±8.98c	1.50±0.015a	10.47±0.41b	1.45±0.033b	79.33±4.62b	360.02±10.16b
方差分析 ANOVA	营养液 F	489.516**	197.361**	318.604**	61.696**	26.000**	0.244	125.693**
	菌剂 M	63.083**	182.428**	33.353**	4.597*	0.153	61.526**	1.797
	营养液×菌剂 F×M	1.696	7.256**	44.611**	21.232**	18.003**	25.139**	33.304**

处理 Treatment		根 Root (g/plant)	茎 Stem (g/plant)	叶 Leaf (g/plant)	果 Fruit (g/plant)	果实干物质分配系数 Distribution index of fruit (%)
CK1		3.52±0.16b	14.00±0.34d	56.81±2.25b	69.30±0.66e	0.48±0.009c
NT1		4.24±0.08a	16.08±0.14c	57.09±3.78b	85.63±1.24c	0.53±0.013ab
NT2		3.57±0.01b	16.61±0.56bc	58.36±2.27b	81.50±1.26d	0.51±0.013bc
CK2		3.38±0.19b	15.75±0.24c	61.99±0.26b	94.34±0.82b	0.54±0.002ab
FT1		4.41±0.24a	20.43±0.71a	70.95±3.81a	106.03±1.10a	0.53±0.011ab
FT2		3.68±0.10b	17.66±0.39b	62.91±1.57ab	105.99±0.43a	0.56±0.006a
方差分析 ANOVA	营养液 F	0.151	44.008**	13.372**	874.705**	17.945**
	菌剂 M	17.263**	30.714**	1.647	122.633**	2.838
	营养液×菌剂 F×M	0.539	7.813**	1.954	3.456	4.494*

处理 Treatment		脲酶 Urease activity (mg·g^-1·d^-1)		蔗糖酶 Sucrase activity (mg·g^-1·d^-1)		过氧化氢酶 Catalase activity (mL·g^-1·d^-1)		碱性磷酸酶 Alkaline phosphatase activity (mg·g^-1·d^-1)
处理 Treatment		盛果期 Full fruiting stage	拉秧期 Seedling pulling stage	盛果期 Full fruiting stage	拉秧期 Seedling pulling stage	盛果期 Full fruiting stage	拉秧期 Seedling pulling stage	盛果期 Full fruiting stage	拉秧期 Seedling pulling stage
CK1		6.53±0.02b	6.78±0.09c	136.86±9.37e	103.36±1.90c	1.93±0.139d	2.36±0.012bc	5.46±0.31c	3.86±0.71b
NT1		5.81±0.04c	6.36±0.02d	274.62±9.00b	83.81±5.17d	2.34±0.003b	2.44±0.009ab	6.59±0.30b	7.53±0.44a
NT2		5.85±0.01c	6.66±0.03c	296.44±1.22a	63.53±8.88e	2.48±0.021b	2.54±0.039a	5.83±0.12c	4.13±0.45b
CK2		6.67±0.03b	6.70±0.03c	160.30±0.37d	96.57±0.80cd	2.03±0.168cd	2.23±0.015d	5.89±0.03c	3.94±0.37b
FT1		6.92±0.11a	7.58±0.09a	270.29±5.32b	127.16±8.72b	2.85±0.018a	2.31±0.033cd	6.95±0.28ab	8.62±0.43a
FT2		7.00±0.10a	7.11±0.05b	200.27±9.24c	157.75±1.71a	2.26±0.006bc	2.46±0.059ab	7.48±0.09a	7.25±0.11a
方差分析 ANOVA	营养液 F	234.482**	127.569**	20.907**	90.592**	0.827	17.273**	2.55	4.972*
	菌剂 M	7.104**	8.047**	182.185**	1.811	23.677**	19.534**	16.378**	1.783
	营养液×菌剂 F×M	39.428**	63.632**	41.375**	40.529**	9.634**	0.466	13.287**	51.432**

处理 Treatment		NH₄⁺-N (mg·kg^-1)		NO₃^--N (mg·kg^-1)		有效态N总量 Total available nitrogen (mg·kg^-1)
处理 Treatment		盛果期 Full fruiting stage	拉秧期Seedling pulling stage	盛果期 Full fruiting stage	拉秧期 Seedling pulling stage	盛果期 Full fruiting stage	拉秧期 Seedling pulling stage
CK1		21.81±0.67d	24.16±0.72b	405.44±5.17e	271.84±2.54f	427.25±5.45e	296.00±3.16f
NT1		75.63±0.69a	20.37±0.23bc	622.19±3.82c	341.12±2.08d	697.81±3.98c	371.41±2.13d
NT2		62.03±2.47b	30.61±2.64a	612.64±18.31c	283.15±4.38e	674.67±20.77c	313.76±5.91e
CK2		15.47±0.51e	30.29±0.37a	514.72±1.64d	389.23±5.04c	530.19±2.14d	409.60±4.94c
FT1		77.39±0.68a	18.67±0.74c	838.24±8.52a	745.07±1.12a	915.63±9.18a	763.73±0.44a
FT2		41.92±0.65c	22.56±0.67b	704.16±7.09b	437.76±3.15b	746.08±7.28b	460.32±3.75b
方差分析 ANOVA	营养液 F	244.174**	1.517	346.006**	5046.852**	223.717**	3769.324**
	菌剂 M	1243.315**	25.307**	467.776**	3411.182**	542.801**	2423.188**
	营养液×菌剂 F×M	180.810**	17.469**	27.105**	975.240**	35.165**	714.535**

因子 Factor	游离氨基酸 Free amino acids	可溶性蛋白 Soluble protein	有机酸 Organic acid	可溶性糖 Soluble sugar	还原糖 Reducing sugar	维生素C Vitamin C	硝酸盐 Nitrate content
脲酶 Urease	0.651**	0.410	0.532*	0.540*	0.312	0.686**	0.408
蔗糖酶 Sucrase	0.166	-0.106	0.296	0.349	0.501*	0.192	-0.053
过氧化氢酶 Catalase	0.426	0.128	0.435	0.652**	0.689**	0.563*	-0.003
碱性磷酸酶 Alkaline phosphatase	0.762**	0.256	0.547*	0.637**	0.467	0.501*	0.320
铵态氮 Ammonium	0.123	0.127	0.094	0.382	0.501*	0.190	-0.150
硝态氮 Nitrate	0.798**	0.315	0.764**	0.879**	0.844**	0.771**	0.340
有效态氮总量 Total available nitrogen	0.728**	0.301	0.693**	0.845**	0.834**	0.716**	0.276

Effects of Two Microbial Agents on Yield, Quality and Rhizosphere Environment of Autumn Cucumber Cultured in Organic Substrate

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