Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (20): 4177-4188.doi: 10.3864/j.issn.0578-1752.2020.20.007

• PLANT PROTECTION • Previous Articles     Next Articles

Effect of Panicle Neck Blast on Grain Yield and Stem Node Metabolites at the Rice Filling Stage

PAN ZhengYan1,2(),LIU Bo1,JIANG HongBo1,YAO JiPan1,BAI YuanJun1(),XU ZhengJin2()   

  1. 1Liaoning Rice Research Institute, Shenyang 110101
    2College of Agriculture, Shenyang Agricultural University, Shenyang 110161
  • Received:2020-03-11 Accepted:2020-05-08 Online:2020-10-16 Published:2020-10-26
  • Contact: YuanJun BAI,ZhengJin XU E-mail:pzhyma@126.com;cycbyj@126.com;xuzhengjin@126.com

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Abstract:

【Objective】The objective of this study is to detect the stem node metabolite of susceptible cultivars which suffered the rice blast under the natural condition, analyze the reason for rice cultivars susceptibility and yield reduction, and to provide a theoretical basis for the susceptible mechanism study of japonica rice and ecological control.【Method】With susceptible cultivar which suffered the rice blast Liaoxing1 and japonica rice restorer C418 as the materials, the yield and quality character including seed setting rate, 1000-grain weight, length, brown rice were measured at the maturation stage. The incidence of panicle neck blast was investigated at the filling stage, and GC-MS was carried out to analyze the metabolites of diseased stem nodes and normal stem nodes, the metabolite composition between diseased stem nodes and normal stem nodes was analyzed by principal components analyses (PCA), the variable weights (VIP>1.0) of partial least-squares discriminant analysis (PLS-DA) were used to screen the species of differential metabolites, the difference of carbohydrate and amino acid metabolites was compared, and the reasons for the decrease of rice yield were analyzed from the metabolites.【Result】The incidence of panicle neck blast of Liaoxing1 and C418 was 57.23% and 82.94%, respectively. The panicle neck blast significantly reduced the head rice rate of the primary branch, the seed set rate, 1000-grain weight, length-width ratio and head rice rate of the secondary branch, there was no significant difference in other characters. 358 metabolites were identified in the two cultivars, the metabolites of diseased stem node increased by 73.46% and 67.88%, respectively. The PCA and PLS-DA analysis could clearly identify the metabolites of diseased stem node and normal stem node. The metabolite phenotypes of Liaoxing 1 and C418 were obviously different between the diseased stem node and normal stem node. Compared with normal stem node, the relative content of 61 metabolites induced by Liaoxing 1 diseased stem nodes changed significantly, and the relative content of 47 metabolites induced by C418 diseased stem nodes changed significantly, there were 30 metabolites in same of the diseased stem nodes of these two cultivars. The relative content of secondary metabolites of vanillic acid, alpha tocopherol, dehydroascorbic acid, triacontanol, daidzein and N-acetyl-d-mannosamine significantly decreased by 87.03%, 96.29%, 77.95%, 74.56%, 86.48% and 82.68% in Liaoxing1, respectively. In C418, it decreased by 99.92%, 99.68%, 97.26%, 86.67%, 94.96% and 76.74%, respectively. The relative content of the above six types of secondary metabolites in normal stem node of Liaoxing1 was 2.64, 2.39, 2.39, 2.27, 3.09 and 2.56 times of that in C418, respectively. The panicle neck blast increased 63.33% of carbohydrate metabolites and 68.42% of amino acid metabolites in stem node, the carbohydrate and amino acid metabolites increased by 1.02-17.92 and 1.05-13.12 times, respectively. Among them, the enhanced amplitude of carbohydrate metabolites in C418 was more significant.【Conclusion】The occurrence of panicle neck blast significantly changed the metabolite composition of susceptible cultivars Liaoxing 1 and C418 stem node, the susceptible cultivar may adjust its defensive reaction to the rice blast following the relative amount change of vanillic acid, alpha tocopherol, dehydroascorbic acid, triacontanol, daidzein and N-acetyl-d-mannosamine. By accumulating a large amount of the carbohydrate and amino acid metabolites, the diseased stem nodes reduce their transport to grains and restrain the formation of yield.

Key words: Oryza sativa, panicle neck blast, metabolite, carbohydrate, amino acid, yield

Table 1

Effect of panicle neck blast on Liaoxing 1 and C418 yield components"

品种
Cultivar		 处理
Treatment		 一次枝梗 Primary branch 二次枝梗 Secondary branch
结实率
Seed setting rate (%)		 千粒重
1000-grain weight (g)		 结实率
Seed setting rate (%)		 千粒重
1000-grain weight (g)
辽星1号
Liaoxing1		 LX1-CK 95.05±4.05 25.39±0.74 89.59±8.91 22.35±0.79
LX1-disease 92.28±7.45 24.64±4.05 84.42±17.52 21.15±3.81
P值P-value 0.0016** 0.11 0.0004** 0.0218*
C418 C418-CK 87.11±5.11 27.54±0.88 81.03±7.04 23.68±1.53
C418-disease 85.00±10.81 25.91±1.78 75.00±34.95 21.74±3.83
P值P-value 0.0899 0.0037** 0.0116* 0.0136*

Table 2

Effect of panicle neck blast on appearance quality of Liaoxing 1 and C418"

品种
Cultivar		 部位
Part		 处理
Treatment		 粒长
Length (mm)		 粒宽
Width (mm)		 长宽比
Length-width ratio		 垩白率
Chalky rice rate (%)
辽星1号Liaoxing1 一次枝梗
Primary branch		 LX1-CK 7.30±0.03 3.16±0.01 2.31±0.01 5.03±0.30
LX1-disease 7.19±0.02 3.18±0.01 2.27±0.01 7.75±1.10
P值P-value 0.0116* 0.6178 0.0525 0.0136*
二次枝梗
Secondary branch		 C418-CK 7.15±0.04 3.04±0.02 2.19±0.01 19.23±1.60
C418-disease 7.04±0.05 3.08±0.04 2.00±0.05 15.36±10.61
P值P-value 0.0413* 0.4243 0.0002** 0.0976
C418 一次枝梗
Primary branch		 LX1-CK 8.50±0.02 3.12±0.01 2.73±0.01 18.92±0.77
LX1-disease 8.42±0.16 3.01±0.02 2.80±0.03 15.59±2.56
P值P-value 0.2781 0.0016** 0.0513 0.1284
二次枝梗
Secondary branch		 C418-CK 8.31±0.03 3.06±0.01 2.07±0.01 29.05±1.22
C418-disease 8.25±0.14 2.96±0.03 1.97±0.03 25.35±4.43
P值P-value 0.4419 0.0104* 0.0115* 0.1404

Table 3

Effect of panicle neck blast on milling quality of Liaoxing 1 and C418 (%)"

品种
Cultivar		 处理
Treatment		 一次枝梗 Primary branch 二次枝梗 Secondary branch
糙米率
Brown rice		 精米率
Milled rice		 整精米率
Head milled rice		 糙米率
Brown rice		 精米率
Milled rice		 整精米率
Head milled rice
辽星1号Liaoxing1 LX1-CK 81.26±0.30 71.19±2.25 69.99±2.21 80.55±3.00 69.81±1.84 67.22±2.25
LX1-disease 80.03±4.54 69.07±2.08 64.44±12.41 79.16±1.63 68.38±6.05 63.76±4.21
P值P-value 0.1964 0.0688 0.0179* 0.1797 0.5054 0.0061**
C418 C418-CK 78.44±0.95 66.47±9.88 62.96±13.91 76.75±3.02 64.88±1.68 63.06±6.15
C418-disease 74.98±13.96 62.41±9.48 57.43±14.50 72.47±4.42 60.93±6.90 56.92±8.05
P值P-value 0.1517 0.0741 0.0081** 0.0956 0.0941 0.0444*

Fig. 1

PCA and PLS-DA analysis of the metabolites between diseased stem nodes and normal stem nodes"

Fig. 2

Hierarchical cluster analysis of differential metabolites resulting in thermal energy maps Centroid method and euclidean distance method are used for cluster analysis. The red rectangle indicates that the metabolite content is significantly up-regulated and the blue rectangle indicates the significant down-regulation of the metabolite content. The same as Fig. 3"

Fig. 3

Thermal energy maps of hierarchical cluster analysis of carbohydrate metabolites and amino acid metabolites"

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