基于转录组测序开发糜子SSR标记

doi:10.3864/j.issn.0578-1752.2020.10.002

Abstract

Abstract:

【Objective】Based on the data of transcriptome sequencing, 200 pairs of SSR primers were detected using six proso millet accessions with different origin. The aim of this study is to construct a set of molecular markers for assessing the genetic diversity of proso millet germplasms.【Method】Primer Premier 5.0 software was used to design primers, DNA was extracted by modified CTAB method and amplified by PCR. Polyacrylamide gel electrophoresis was used to screen the polymorphism of primers. Genetic diversity parameters were calculated with PowerMarker 3.25 and PopGen 1.32.【Result】Ninety seven and eighty out of 200 pairs of primers were shown monomorphic and polymorphic, respectively. A total of 20 were mono-nucleotide SSRs, among them 10 were polymorphic with repeat motif A (50%) and T (50%). A total of 36 were di-nucleotide SSRs, among them 15 were polymorphic with seven types of repeat motif, AG class was the most frequent, followed by the TC, GC and GA, CA, TA and AC were the least. A total of 144 were tri-nucleotide SSRs, among them 55 were polymorphic with 24 types of repeat motif, GGC, GCG and GCC class were the most frequent, followed by the GAA, GCT, CGC, etc, ACC, AGG, CAG, CGT, AAG, AAC, TCG, CGA, ATT, CAA and CCA were the least. In terms of the mono-, di-, tri-nucleotide SSR, their Rp value ranged from 0.67 to 4.67 (mean 2.07), 1.33 to 4.33 (mean 2.73) and 0.67 to 4.00 (mean 1.83), respectively. Evaluating the distribution frequency based on Rp, 80 SSRs were divided into five intervals viz 0-1, 1-2, 2-3, 3-4 and 4-5, with 17 (21.25%), 36 (45.00%), 11 (13.75%), 14 (17.50%) and 2 (2.50%) markers, respectively. For the mean of the Rp value, the mono-, di-, tri-nucleotide SSR were 0.33 to 0.67 (average 0.51), 0.40 to 0.78 (average 0.59) and 0.33 to 0.83 (average 0.59), respectively. As for mono-nucleotide SSRs, a total of 22 allelic variations were detected with 2-3 alleles (average 2.2000) for each locus; 2 and 3 alleles were identified for 8 and 2 loci, respectively. In terms of di-nucleotide SSRs, a total of 38 allelic variations were generated with 2-3 alleles (average 2.5333) for each locus; 7 and 8 loci produced 2 and 3 alleles, respectively. As for tri-nucleotide SSRs, a total of 136 allelic variations were generated with 2-3 alleles (average 2.4727) for each locus; 29 and 26 loci produced 2 and 3 alleles, respectively. In terms of the polymorphism information content, the mono-, di-, tri-nucleotide SSR were 0.3750-0.5355, 0.2392-0.7438 and 0.2392-0.7438, respectively. In terms of the diversity index, the mono-, di-, tri-nucleotide SSR were 0.6365-1.0776 (average 0.7497), 0.5623-1.0986 (average 0.8339) and 0.5623-1.0889 (average 0.8312), respectively. 【Conclusion】Two hundreds pairs of SSR primers obtained from transcriptome sequencing were used to detect genetic diversity of 6 proso millet accessions. It was shown that 177 (88.5%) out of the 200 pairs primers amplified intact bands, and 80 SSRs appeared polymorphic with the polymorphic rate 40%.

Key words: proso millet (Panicum miliaceun L.), SSR, resolving power (Rp), polymorphism information content (PIC), diversity index

CHEN XiaoHong,HE JieLi,SHI TianTian,SHAO HuanHuan,WANG HaiGang,CHEN Ling,GAO ZhiJun,WANG RuiYun,QIAO ZhiJun. Developing SSR Markers of Proso Millet Based on Transcriptome Sequencing[J].Scientia Agricultura Sinica, 2020, 53(10): 1940-1949.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2020.10.002

https://www.chinaagrisci.com/EN/Y2020/V53/I10/1940

Figures/Tables 7

Table 1

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

References 45

[1]	VETRIVENTHAN M, AZEVEDO V C R, UPADHYAYA H D, NARESH D . Variability in the global proso millet ( Panicum miliaceum L.) germplasm collection conserved at the ICRISAT GenBank. Agriculture, 2019,9:112.
[2]	国家谷子糜子产业技术体系. 中国现代农业产业可持续发展战略研究·谷子糜子分册. 北京: 中国农业出版社, 2018.
	CHINA AGRICULTURE RESEARCH SYSTEM of FOXTAIL MILLET and PROSO MILLET. Sustainable Development Strategy of Modern Agricultural Industry in China: Foxtail Millet and Proso Millet Beijing: China Agriculture Press, 2018. (in Chinese)
[3]	王瑞云 . 糜子遗传多样性及进化研究进展. 北京: 中国农业出版社, 2017.
	WANG R Y. Genetic Diversity and Evolution Advancement in Common Millet (Panicum miliaceum L.). Beijing: China Agriculture Press, 2017. (in Chinese)
[4]	HABIYAREMYE C, MATANGUIHAN J B, GUEDES J D, GANJYAL G M, WHITEMAN M R, KIDWELL K K, MURPHY K M . Proso millet ( Panicum miliaceum L.) and its potential for cultivation in the Pacific Northwest, U.S.: A review. Frontiers in Plant Science, 2017,7:1961.
[5]	DIAO X M . Production and genetic improvement of minor cereals in China. The Crop Journal, 2017,5(2):103-114.
[6]	WANG R Y, HUNT H V, QIAO Z J, WANG L, HAN Y H . Diversity and cultivation of broomcorn millet ( Panicum miliaceum L.) in China: A review. Economic Botany, 2016,70(3):332-342.
[7]	CAVERS P B, KANE M . The biology of Canadian weeds: 155 Panicum miliaceum L. Canadian Journal of Plant Sciences, 2016,96:939-988.
[8]	CHANGMEI S, DOROTHY J . Millet-the frugal grain. International Journal of Scientific Research and Reviews, 2014,3(4):75-90.
[9]	COULIBALY A, KOUAKOU B, CHEN J . Phytic acid in cereal grains: structure healthy or harmful ways to reduce phytic acid in cereal grains and their effects on nutritional quality. American Journal of Plant Nutrition and Fertilization Technology, 2011. 1(1):1-22.
[10]	HUNT H V, RUDZINSKI A, JIANG H E, WANG R Y, THOMAS M G, JONES M K . Genetic evidence for a western Chinese origin of broomcorn millet ( Panicum miliaceum). The Holocene, 2018,28(12):1968-1978. doi: 10.1177/0959683618798116
[11]	WANG R Y, WANG H G, LIU X H, JI X, CHEN L, LU P, LIU M X, TENG B, QIAO Z J . Waxy allelic diversity in common millet (Panicum miliaceum L.) in China. The Crop Journal, 2018,6(4):377-385.
[12]	WANG R Y, WANG H G, LIU X Y, LIAN S, CHEN L, QIAO Z J, MCINERNEY C E, WANG L . Drought-induced transcription of resistant and sensitive common millet varieties. The Journal of Animal & Plant Sciences, 2017,27(4):1303-1314.
[13]	薛延桃, 陆平, 乔治军, 刘敏轩, 王瑞云 . 基于SSR标记的黍稷种质资源遗传多样性及亲缘关系研究. 中国农业科学, 2018,51(15):2846-2859.
	XUE Y T, LU P, QIAO Z J, LIU M X, WANG R Y . Genetic diversity and genetic relationship of broomcorn millet ( Panicum miliaceum L.) germplasm based on SSR markers. Scientia Agricultura Sinica, 2018,51(15):2846-2859. (in Chinese)
[14]	王瑞云, 季煦, 陆平, 刘敏轩, 许月, 王纶, 王海岗, 乔治军 . 利用荧光SSR分析中国糜子遗传多样性. 作物学报, 2017,43(4):530-548.
	WANG R Y, JI X, LU P, LIU M X, XU Y, WANG L, WANG H G, QIAO Z J . Analysis of genetic diversity in common millet (Panicum miliaceum) using fluorescent SSR in Chinese. Acta Agronomica Sinica, 2017,43(4):530-548. (in Chinese)
[15]	王璐琳, 王瑞云, 何杰丽, 薛延桃, 陈凌, 王海岗, 乔治军 . 糜子特异性SSR标记的开发. 山西农业科学, 2018,46(1):1-4, 86.
	WANG L L, WANG R Y, HE J L, XUE Y T, CHEN L, WANG H G, QIAO Z J . Development of specific SSR marker in common millet. Journal of Shanxi Agricultural Sciences, 2018,46(1):1-4, 86. (in Chinese)
[16]	董俊丽, 王海岗, 陈凌, 王君杰, 曹晓宁, 王纶, 乔治军 . 糜子骨干种质遗传多样性和遗传结构分析. 中国农业科学, 2015,48(16):3121-3131.
	DONG J L, WANG H G, CHEN L, WANG J J, CAO X N, WANG L, QIAO Z J . Analysis of genetic diversity and structure of proso millet core germplasm. Scientia Agricultura Sinica, 2015,48(16):3121-3131. (in Chinese)
[17]	FLAJŠMAN M, ŠTAJNER N, AČKO D K . Genetic diversity and agronomic performance of Slovenian landraces of proso millet ( Panicum miliaceum L.). Turkish Journal of Botany, 2019,43(2):185-195.
[18]	赖勇, 王鹏喜, 范贵强, 司二静, 王晋, 杨轲, 孟亚雄, 李葆春, 马小乐, 尚勋武, 王化俊 . 大麦SSR标记遗传多样性及其与农艺性状关联分析. 中国农业科学, 2013,46(2):233-242. doi: 10.3864/j.issn.0578-1752.2013.02.002
	LAI Y, WANG P X, FAN G Q, SI E J, WANG J, YANG K, MENG Y X, LI B C, MA X L, SHANG X W, WANG H J . Genetic diversity and association analysis using SSR markers in barley. Scientia Agricultura Sinica, 2013,46(2):233-242. (in Chinese) doi: 10.3864/j.issn.0578-1752.2013.02.002
[19]	王果, 胡正, 张保缺, 王成社, 张辉 . 山西省野生大豆资源遗传多样性分析. 中国农业科学, 2008,41(7):2182-2190.
	WANG G, HU Z, ZHANG B Q, WANG C S, ZHANG H . Genetic diversity analysis of Shanxi’s wild soybean ( Glycine soja). Scientia Agricultura Sinica, 2008,41(7):2182-2190. (in Chinese)
[20]	DAS S, KHOUND R, SANTRA M, SANTRA D K . Beyond bird feed: proso millet for human health and environment. Agriculture, 2019,9(3):64.
[21]	陈雪燕, 王亚娟, 雒景吾, 吉万全 . 陕西省小麦地方品种主要性状的遗传多样性研究. 麦类作物学报, 2007,27(3):456-460.
	CHEN X Y, WANG Y J, LUO J W, JI W Q . Genetic diversity in main characters of wheat landraces in Shaanxi province. Journal of Triticeae Crops, 2007,27(3):456-460. (in Chinese)
[22]	HU X Y, WANG J F, LU P, ZHANG H S . Assessment of genetic diversity in broomcorn millet ( Panicum miliaceum L.) using SSR markers. Journal of Genetics and Genomics, 2009,36:491-500. doi: 10.1016/S1673-8527(08)60139-3
[23]	CHO Y I, CHUNG J W, LEE G A, MA K H, DIXIT A, GWAG J G, PARK Y J . Development and characterization of twenty-five new polymorphic microsatellite markers in proso millet (Panicum miliaceum L.). Genes & Genomics, 2010,32(3):267-273.
[24]	RAJPUT S G, HARVESON T, SANTRA D K . Development and characterization of SSR markers in proso millet ( Panicum miliaceum L.) based on switchgrass genomics. American Journal of Plant Sciences, 2014,5(1):175-186. doi: 10.4236/ajps.2014.51023
[25]	王银月, 刘敏轩, 陆平, 乔治军, 杨天育, 李海, 崔喜艳 . 构建黍稷分子遗传图谱SSR引物的筛选. 作物杂志, 2014,4(8):32-38.
	WANG Y Y, LIU M X, LU P, QIAO Z J, YANG T Y, LI H, CUI X Y . The SSR marker selection of broomcorn millet (Panicum miliaceum L.) for construction of genetic linkage map. Crops, 2014,4:32-38. (in Chinese)
[26]	连帅, 陆平, 乔治军, 张琦, 张茜, 刘敏轩, 王瑞云 . 利用SSR分子标记研究国内外黍稷地方品种和野生资源的遗传多样性. 中国农业科学, 2016,49(17):3264-3275. doi: 10.3864/j.issn.0578-1752.2016.17.002
	LIAN S, LU P, QIAO Z J, ZHANG Q, ZHANG Q, LIU M X, WANG R Y . Genetic diversity in broomcorn millet ( Panicum miliaceum L.) from China and abroad by using SSR markers. Scientia Agricultura Sinica, 2016,49(17):3264-3275. (in Chinese) doi: 10.3864/j.issn.0578-1752.2016.17.002
[27]	刘笑瑜 . 利用高基元SSR分析中国糜子资源的遗传多样性[D]. 太谷: 山西农业大学, 2017.
	LIU X Y . Analysis of genetic diversity of Chinese proso millet resources by high motif SSR[D]. Taigu: Shanxi Agricultural University, 2017. (in Chinese)
[28]	JIANG Y M, LI H Q, ZHANG J N, XIANG J Y, CHENG R H, LIU G Q . Whole genomic EST-SSR development based on high-throughput transcript sequencing in proso millet ( Panicum miliaceum L.). International Journal of Agriculture & Biology, 2018,20(3):617-620.
[29]	寇淑君, 霍阿红, 付国庆, 纪军建, 王瑶, 左振兴, 刘敏轩, 陆平 . 利用荧光SSR分析中国糜子的遗传多样性和群体遗传结构. 中国农业科学, 2019,52(9):1475-1487. doi: 10.3864/j.issn.0578-1752.2019.09.001
	KOU S J, HUO A H, FU G Q, JI J J, WANG Y, ZUO Z X, LIU M X, LU P . Genetic diversity and population structure of broomcorn millet in China based on fluorescently labeled SSR. Scientia Agricultura Sinica, 2019,52(9):1475-1487. (in Chinese) doi: 10.3864/j.issn.0578-1752.2019.09.001
[30]	石甜甜, 何杰丽, 高志军, 陈凌, 王海岗, 乔治军, 王瑞云 . 利用EST-SSR评估糜子资源遗传差异. 中国农业科学, 2019,52(22):4100-4109.
	SHI T T, HE J L, GAO Z J, CHEN L, WANG H G, QIAO Z J, WANG R Y . Genetic diversity of common millet resources assessed with EST-SSR markers. Scientia Agricultura Sinica, 2019,52(22):4100-4109. (in Chinese)
[31]	何杰丽, 石甜甜, 陈凌, 王海岗, 高志军, 杨美红, 王瑞云, 乔治军 . 糜子EST-SSR分子标记的开发及种质资源遗传多样性分析. 植物学报, 2019,54(6):723-732.
	HE J L, SHI T T, CHEN L, WANG H G, GAO Z J, YANG M H, WANG R Y, QIAO Z J . The genetic diversity of common millet ( Panicum miliaceum) germplasm resources based on the EST-SSR markers. Chinese Bulletin of Botany, 2019,54(6):723-732. (in Chinese)
[32]	翟中会, 陈希南, 王娟 . 利用Primer Premier 5.0进行引物设计. 西北医学教育, 2008,16(4):695-698.
	ZHAI Z H, CHEN X N, WANG J . Primer design with Primer Premier 5.0. Northwest Medical Education, 2008,16(4):695-698. (in Chinese)
[33]	EDWARDS K, JOHNSTONE C, THOMPSON C . A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Research, 1991,19(6):1349.
[34]	PREVOST A, WILKINSON M J . A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics, 1999,98(1):107-112.
[35]	LIU K, MUSE S V . PowerMarker: Integrated analysis environment for genetic marker data. Bioinformatics, 2005,21:2128-2129. doi: 10.1093/bioinformatics/bti282
[36]	YEH F C, BOYLE T J . Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgian Journal of Botany, 1997,129:157.
[37]	HU Y G, ZHU J, LIU F, ZHANG Z, CHAI Y, WEINING S . Genetic diversity among Chinese landraces and cultivars of broomcorn millet ( Panicum miliaceum) revealed by the polymerase chain reaction. Annals of Applied Biology, 2008,153(3):357-364.
[38]	SATYA P, KARAN M, JANA S, MITRA S, SHARMA A, KARMAKAR P G, RAY D P . Start codon targeted (SCoT) polymorphism reveals genetic diversity in wild and domesticated populations of ramie ( Boehmeria nivea L. Gaudich.), a premium textile fiber producing species. Meta Gene, 2015,3:62-70. doi: 10.1016/j.mgene.2015.01.003
[39]	SATYA P, BANERJEE R, KARAN M, MUKHOPADHYAY E, CHAUDHARY B, BERA A, MARUTHI R T, SARKAR S K . Insight into genetic relation and diversity of cultivated and semi-domesticated under-utilized Crotalaria species gained using start codon targeted (SCoT) markers. Biochemical Systematics and Ecology, 2016,66:24-32. doi: 10.1016/j.bse.2016.02.032
[40]	BHATTACHARYYA P, KUMARIA S, KUMAR S, TANDON P . Start Codon Targeted (SCoT) marker reveals genetic diversity of Dendrobium nobile Lindl., an endangered medicinal orchid species. Gene, 2013,529(1):21-26. doi: 10.1016/j.gene.2013.07.096
[41]	TIWARI G, SINGH R, SINGH N, CHOUDHURY D R, PALIWAL R, KUMAR A, GUPTA V . Study of arbitrarily amplified (RAPD and ISSR) and gene targeted (SCoT and CBDP) markers for genetic diversity and population structure in Kalmegh [Andrographis paniculata (Burm. f.) Nees]. Industrial Crops and Products, 2016,86:1-11.
[42]	黄建敏 . 青篱柴转录组高通量测序分析及SSR分子标记的开发[D]. 贵阳: 贵州师范大学, 2018.
	HUANG J M . High-throughput sequencing analysis and development of SSR molecular markers in the transcriptome of Tirpitzia sinensis[D]. Guiyang: Guizhou Normal University, 2018. (in Chinese)
[43]	HU Z, ZHANG T, GAO X X, WANG Y, ZHANG Q, ZHOU H J, ZHAO G F, WANG M L, WOESTE K E, ZHAO P . De novo assembly and characterization of the leaf, bud, and fruit transcriptome from the vulnerable tree Juglans mandshurica for the development of 20 new microsatellite markers using Illumina sequencing. Molecular Genetics and Genomics, 2016,291(2):849-862.
[44]	BISWAS M K, CHAI L J, MAYER C, XU Q, GUO W W, DENG X X . Exploiting BAC-end sequences for the mining, characterization and utility of new short sequences repeat (SSR) markers in Citrus. Molecular Biology Reports, 2012,39(5):5373-5386. doi: 10.1007/s11033-011-1338-5
[45]	张文芳 . 基于怀山药转录组测序的SSR分子标记的开发与应用[D]. 新乡: 河南师范大学, 2018.
	ZHANG W F . The development and application of SSR molecular markers from Dioscorea oppostia Thumb. based on transcriptomic sequencing[D]. Xinxiang: Henan Normal University, 2018. (in Chinese)

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

序号 No.	统一编号 Unicode	名称 Accession name	来源 Origin	生态栽培区 Ecotope
1	00000177	红糜子Hongmizi	黑龙江省宁安市 Ning’an, Heilongjiang	东北春糜子区 Northeast spring-sowing ecotope
2	00000750	白糜子Baimizi	新疆沙湾县 Shawan, Xinjiang	西北春夏糜子区 Northwest spring & summer-sowing ecotope
3	00006653	金黍Jinshu	海南省 Hainan	南方秋冬糜子区 Southern autumn & winter-sowing ecotope
4	00007238	大红糜子Dahongmizi	内蒙古巴彦淖尔 Bayanzhuoer, Inner Mongolia	北方春糜子区 Northern spring-sowing ecotope
5	00007478	白圪塔糜Baigetami	青海省湟中县 Huangzhong, Qinghai	西北春夏糜子区 Northwest spring & summer-sowing ecotope
6		红黍子Hongshuzi	河南省安阳市 Anyang, Henan	华北夏糜子区 Northern summer-sowing ecotope

Developing SSR Markers of Proso Millet Based on Transcriptome Sequencing

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 7

References 45

Related Articles 15

Metrics

Comments

Recommended 0

[1]	ZHU YanSong,ZHANG YaFei,CHENG Li,YANG ShengNan,ZHAO WanTong,JIANG Dong. Identification of 60 Citrus Accessions Using Target SSR-seq Technology [J]. Scientia Agricultura Sinica, 2022, 55(22): 4458-4472.
[2]	HU GuangMing,ZHANG Qiong,HAN Fei,LI DaWei,LI ZuoZhou,WANG Zhi,ZHAO TingTing,TIAN Hua,LIU XiaoLi,ZHONG CaiHong. Screening and Application of Universal SSR Molecular Marker Primers in Actinidia [J]. Scientia Agricultura Sinica, 2022, 55(17): 3411-3425.
[3]	YANG Cheng,GONG GuiZhi,PENG ZhuChun,CHANG ZhenZhen,YI Xuan,HONG QiBin. Genetic Relationship Among Citrus and Its Relatives as Revealed by cpInDel and cpSSR Marker [J]. Scientia Agricultura Sinica, 2022, 55(16): 3210-3223.
[4]	WANG LuWei,SHEN ZhiJun,LI HeHuan,PAN Lei,NIU Liang,CUI GuoChao,ZENG WenFang,WANG ZhiQiang,LU ZhenHua. Analysis of Genetic Diversity of 79 Cultivars Based on SSR Fluorescence Markers for Peach [J]. Scientia Agricultura Sinica, 2022, 55(15): 3002-3017.
[5]	CHEN Xu,HAO YaQiong,NIE XingHua,YANG HaiYing,LIU Song,WANG XueFeng,CAO QingQin,QIN Ling,XING Yu. Association Analysis of Main Characteristics of Bur and Nut with SSR Markers in Chinese Chestnut [J]. Scientia Agricultura Sinica, 2022, 55(13): 2613-2628.
[6]	SUN Yue,YANG HuiMin,HE RongRong,ZHANG JunXiang. Implantation and Persistence of Inoculated Active Dry Yeast in Industrial Wine Fermentations [J]. Scientia Agricultura Sinica, 2021, 54(9): 2006-2016.
[7]	NIE XingHua, ZHENG RuiJie, ZHAO YongLian, CAO QingQin, QIN Ling, XING Yu. Genetic Diversity Evaluation of Castanea in China Based on Fluorescently Labeled SSR [J]. Scientia Agricultura Sinica, 2021, 54(8): 1739-1750.
[8]	HU DongMei,JIANG Dong,LI YongPing,PENG Lei,LI DongYun,ZHU YanSong,YANG YunGuang. Identification of Bud Sport Mutation of Satsuma Mandarin by Target SSR-seq Technology [J]. Scientia Agricultura Sinica, 2021, 54(23): 5083-5096.
[9]	WANG Yan,FAN BaoJie,CAO ZhiMin,ZHANG ZhiXiao,SU QiuZhu,WANG Shen,WANG XueQing,PENG XiuGuo,MEI Li,WU YuHua,LIU ShaoXing,TIAN ShengMin,XU JunJie,JIANG ChunZhi,WANG WeiJuan,LIU ChangYou,TIAN Jing. Quantitative Trait Locus Mapping of Bruchids Resistance Based on A Novel Genetic Linkage Map in Cowpea (Vigna unguiculata) [J]. Scientia Agricultura Sinica, 2021, 54(22): 4740-4749.
[10]	Xue CHEN,Rui WANG,FuYu JING,ShengSen ZHANG,LeDong JIA,MouZheng DUAN,Yu WU. Location and Linkage Markers for Candidate Interval of the White Petal Gene in Brassica napus L. by Next Generation Sequencing [J]. Scientia Agricultura Sinica, 2020, 53(6): 1108-1117.
[11]	ZhiJun XU,Sheng ZHAO,Lei XU,XiaoWen HU,DongSheng AN,Yang LIU. Discovery of Microsatellite Markers from RNA-seq Data in Cultivated Peanut (Arachis hypogaea) [J]. Scientia Agricultura Sinica, 2020, 53(4): 695-706.
[12]	KOU ShuJun, HUO AHong, FU GuoQing, JI JunJian, WANG Yao, ZUO ZhenXing, LIU MinXuan, LU Ping. Genetic Diversity and Population Structure of Broomcorn Millet in China Based on Fluorescently Labeled SSR [J]. Scientia Agricultura Sinica, 2019, 52(9): 1475-1477.
[13]	CHANG JiaYing,LIU ShuSen,MA HongXia,SHI Jie,GUO Ning,ZHANG HaiJian. Genetic Diversity Analysis of Curvularia lunata in Summer Maize in Huang-Huai-Hai Region [J]. Scientia Agricultura Sinica, 2019, 52(5): 822-836.
[14]	WU BingChao, TONG Lei, DU ZhaoChang, HU JiaLing, ZHANG Huan, CHEN Yi, LIU Wei, ZHANG XinQuan, HUANG LinKai. Mutagenic Effects of ⁶⁰Co-γ-rays on Two Species of Pennisetum Genus Forages [J]. Scientia Agricultura Sinica, 2019, 52(3): 414-427.
[15]	JianFeng LI,Bo ZHANG,JianZhang QUAN,YongFang WANG,XiaoMei ZHANG,Yuan ZHAO,XiLei YUAN,XiaoPing JIA,ZhiPing DONG. Associated Loci Detection and Elite Allelic Variations Analysis of Main Agronomic Traits in Foxtail Millet (Setaria italica L.) Based on SSR Markers [J]. Scientia Agricultura Sinica, 2019, 52(24): 4453-4469.