外源一氧化氮对盐胁迫下高粱种子萌发及淀粉转化的影响

doi:10.3864/j.issn.0578-1752.2019.22.016

Abstract

Abstract:

【Objective】To discuss the physiological and biochemical regulation of exogenous Nitric Oxide (NO) on the germination of sorghum seeds under salt stress, which provided a theoretical basis for revealing the germination physiology and chemical regulation of sorghum seeds. 【Method】Sorghum (variety: Jintian 08-1) seeds were cultivated with 0, 50, 100, 150, 200, 300, and 400 mmol·L ^-1 NaCl solution. According to the germination rate under different concentrations of NaCl, the suitable salt tolerance concentration, semi-lethal concentration, and limiting concentration of sorghum seeds at the germination stage were defined. Sorghum seeds pretreated with 0.05, 0.1, 0.2, 0.4, 0.6 and 0.8 mmol·L ^-1 sodium nitroprusside (SNP, NO donor) for 12 h at 25℃ in dark, then were cultured in salt solution of 150 mmol·L ^-1 NaCl. The germination potential and germination rate were counted at cultured 36 h and 72 h, respectively. Proline content, malondialdehyde content and starch transformation related indexes were determined at cultured 5 days. Dinitrosalicylic acid is used in colorimetric determination of reducing sugars and to analyze amylase activity by quantitation of enzymatically released reducing sugar. The content of soluble sugar and starch were determined by anthrone method. Proline content and malondialdehyde (MDA) content were measured by acid-ninhydrin method and thiobarbituric acid method, respectively. The germination rate, germination energy, water absorption capacity of seeds, amylase activity, starch and sugar content, proline and other indexes were determined and analyzed to investigate the effects of exogenous NO on sorghum seed germination and starch transformation under salt stress. 【Result】The germination of sorghum seeds was obviously inhibited by more than 100 mmol·L ^-1 NaCl. When NaCl concentration was 150 mmol·L ^-1, the germination rate of sorghum seeds was 63.17%. 400 mmol·L ^-1 NaCl completely inhibited sorghum seeds germination. Pretreatment with SNP greatly relieves the inhibitory effect of the following salt stress to sorghum seeds germination, especially during the early stage of germination (36 h). 0.05 mmol·L ^-1 SNP alleviated the inhibition of salt stress on seed germination, seed germination potential, germination rate and germination index were 14.44%, 12.22% and 18.07% higher than those of the control, respectively (P<0.05). SNP increased the content of proline and soluble sugar in sorghum seeds by 18.97% and 41.43% respectively, which reduced osmotic potential, promoted water absorption and alleviated osmotic stress caused by NaCl. At the same time, the content of MDA decreased by 17.79% compared with NaCl treatment alone. Further investigations showed that pretreatment with NO donor dramatically stimulated the activities of amylase under salt stress by 17.20% compared with NaCl on the first day after treatment, and accelerated the degradation of starch, increased the content of reducing sugar. By the 5th day of SNP+NaCl treatment, the starch content decreased by 19.17%, and the content of soluble sugar and reducing sugar increased by 41.4% and 41.0%, respectively, compared to NaCl treatment. These newly produced substances provided energy for seed germination, and improved the salt resistance of sorghum seeds during germination period. 【Conclusion】According to our results, exogenous NO could regulate the amylase activity and osmotic regulation ability of sorghum seeds during germination period, improved their resistance to salt stress, and promoted seed germination.

Key words: sorghum, salt stress, nitric oxide, seed germination, starch conversion

YIN MeiQiang,WANG Dong,WANG JinRong,LAN Min,ZHAO Juan,DONG ShuQi,SONG Xi’E,ALAM Sher,YUAN XiangYang,WANG YuGuo,WEN YinYuan. Effects of Exogenous Nitric Oxide on Seed Germination and Starch Transformation of Sorghum Seeds Under Salt Stress[J].Scientia Agricultura Sinica, 2019, 52(22): 4119-4128.

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处理 Treatment	发芽势 Germination energy (%)	发芽率 Germination rate (%)	发芽指数 Germination index	活力指数 Vigor index
CK	88.89±5.09a	90.00±5.77a	15.32±1.16a	0.83±0.08a
NaCl	65.56±1.92c	72.22±3.85bcd	9.35±0.18c	0.18±0.02cd
0.05 mmol·L^-1SNP+NaCl	80.00±5.77b	84.44±9.62ab	11.04±1.01b	0.31±0.03b
0.1 mmol·L^-1SNP+NaCl	70.00±3.33c	78.89±8.39abc	9.64±0.42c	0.19±0.02c
0.2 mmol·L^-1SNP+NaCl	68.89±8.39c	78.89±9.62abc	8.61±0.58cd	0.16±0.01cd
0.4 mmol·L^-1SNP+NaCl	63.33±3.33cd	67.78±8.39cd	7.66±1.11de	0.12±0.03de
0.6 mmol·L^-1SNP+NaCl	55.56±3.85de	64.44±6.94d	7.16±0.38e	0.09±0.01e
0.8 mmol·L^-1SNP+NaCl	53.33±3.33e	63.33±3.33d	6.61±0.76e	0.07±0.01e

Effects of Exogenous Nitric Oxide on Seed Germination and Starch Transformation of Sorghum Seeds Under Salt Stress

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