油菜素内酯对玉米叶片捕光、CO2固定及有机物运输的影响

doi:10.3864/j.issn.0578-1752.2017.21.017

Abstract

Abstract: 【Objective】The objective of this study is to clear the influence and the molecular mechanism of brassinosteroid on the photosynthetic characteristics of action in maize (Zea mays L.) leaves, and then to provide the theoretical basis and technical references for efficient using of brassinosteroid in maize field. 【Method】We sprayed 100 nmol·L^-1 brassinosteroid on maize of 8 leaves period, then observed and analyzed the chloroplast structure, starch accumulation, chlorophyll content, phosphoenol plyruvate carboxylase (PEPC), photosynthetic rate, the activity of sucrose phosphate synthase (SPS) and sucrose synthase (SS) in leaves. 【Result】After 15 days from brassinosteroid treatment, compared with the control treatment, the net photosynthetic rate increased by 32.6%. At the same time, the accumulation of starch grains in the chloroplast was increased significantly. Compared with the control, the chlorophyll content of the leaves treated with brassinosteroid increased by 28.57%. The above results showed that brassinosteroid treatment can improve the light-harvesting ability of maize leaves. PEPC is an enzyme that catalyzes the fixation of CO₂ in C₄ plants. The results showed that brassinosteroid treatment can improve the activity of PEPC in maize leaves, and the activity of PEPC increased by 14.52% compared with the control. This showed that brassinosteroid treatment can improve the ability of fixing CO₂ of maize leaves. Transportation of photosynthetic products is an important factor to determine yield. Through the ultrastructural observation of the phloem tissue cells of maize leaves, it was found that the cell inclusions in the phloem conducting tissue increased under brassinosteroid treatment. Sucrose is the main transportation form of photosynthetic products. Sucrose synthase and sucrose phosphate synthase are key enzymes in sucrose synthesis, and their activity can reflect the transport capacity and strength of photosynthetic products input to grain. In this study, the activity of sucrose synthase and sucrose phosphate synthase increased by 28.26% and 30.20%, respectively, under brassinosteroid treatment. The results showed that brassinosteroid treatment could improve the output ability of maize photosynthetic products.【Conclusion】 Brassinosteroid can raise the light energy utilization of maize blades by raising the photosynthetic pigment content, can enhance the capacity of maize leaf CO₂ fixation by raising the PEP carboxylase activity, and can promote the transformation and transportation of organic matter in maize leaf through raising the activity of sucrose synthase and sucrose phosphatase.

Key words: brassinosteroid, light-harvesting ability, CO₂ fixation, organic matter transport, maize

ZANG JinPing, ZHAO AiJia, ZHAO YaLin, YAN QingDi, FENG JiaJia, ZHANG HaiLi, WANG FengRu, DONG JinGao. The Influence of Brassinosteroid on the Light Catching, CO2 Fixation and the Translocation of Organic Compounds in Maize Leaves[J].Scientia Agricultura Sinica, 2017, 50(21): 4228-4234.

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The Influence of Brassinosteroid on the Light Catching, CO2 Fixation and the Translocation of Organic Compounds in Maize Leaves

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