Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (6): 1098-1108.doi: 10.3864/j.issn.0578-1752.2017.06.011

• HORTICULTURE • Previous Articles     Next Articles

Analysis of the Genetic Diversity of Wild Rosa roxburghii Populations in Guizhou Province Based on EST-SSR Marker

ZHANG Huaishan1, YAN Xiuqin1, LU Min1, WANG Daoping2, AN Huaming1   

  1. 1College of Agriculture of Guizhou University/Guizhou Engineering Research Center for Fruit Crops, Guiyang 550025; 2The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002
  • Received:2016-08-12 Online:2017-03-16 Published:2017-03-16

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Abstract: 【Objective】Genetic structure and genetic diversity of wild Rosa roxburghii resources were analyzed and evaluated in order to provide a scientific basis of the protection and excavation of R. roxburghii resources.【Method】Ten EST-SSR primers with good amplification and high polymorphism were used to analyze the genetic diversity and genetic structure within 12 populations including 255 R. roxburghii germplasms by POPGENE 1.31 software. According to Nei’s standard genetic distance, NTSYS-pc2.10e software was used to cluster the populations, and the Mantel correlation relationship between Nei's genetic distance and geographical distance was tested.【Result】The range of Shannon’s information index (I) was from 0.62 to 0.88, Nei’s gene diversity from 0.40 to 0.52. Total percentage of polymorphic loci (P) was 100% in these populations, except for Fuquan and Qinglong populations. The Chi-square test results showed that the 12 populations of R. roxburghii did not follow with the Hardy-Weinberg Equilibrium at most loci, and the Fit and Fis values were negative. Characteristics, such as Fst =0.0403, gene flow Nem=5.9484, genetic identity (0.9068<GI<0.9926) and genetic distance (GD<0.0978) were discovered among the 12 populations. Futhermore, Mantel test revealed a significant correlation between genetic and geographic distance among populations (r=0.2498, P=0.9512).【Conclusion】The 10 pairs of EST-SSR primers are highly polymorphic genetic markers which can be used effectively for evaluating genetic structure and genetic diversity of R. roxburghii. R. roxburghii in Guizhou province has moderate to high diversity. The vast majority of genetic variation and an excess of heterozygosity occur within populations. And among the populations, it has the characteristics of frequent gene exchange, high genetic consistency and small Nei 's genetic distance.

Key words: Rosa roxburghii, SSR, genetic diversity, population genetic structure

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