,bagging,transcriptome,high-throughput sequencing,functional classification,"/> Effects of Shading Fruit with Opaque Paper Bag on Transcriptome in Peach

Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (6): 1088-1097.doi: 10.3864/j.issn.0578-1752.2017.06.010

• HORTICULTURE • Previous Articles     Next Articles

Effects of Shading Fruit with Opaque Paper Bag on Transcriptome in Peach

HE Ping, LI LinGuang, WANG HaiBo, CHANG YuanSheng, LI HuiFeng   

  1. Shandong Institute of Pomology, Tai’an 271000, Shandong
  • Received:2016-07-01 Online:2017-03-16 Published:2017-03-16

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Abstract: 【Objective】 The objective of this study was to find the transcriptome differences between shading fruits with opaque paper bags and CK in peach, and enrich the peach transcriptome data. 【Method】 Selecting peach fruits as samples (shading fruits with opaque paper bags and CK, respectively), then the transcriptome libraries of peach fruits were constructed by using the Illumina HiSeqTM 2500 sequencing technique, and were analyzed by using the bioinformatics methods subsequently, such as sequencing assess and gene function annotation. 【Result】 The results showed that the 16.62 Gb transcriptome data were obtained from both the shading bagged fruits and CK , and the base ratios with quality values higher than 30 in reads (Q30) were more than 91% from both the shading fruits and CK. There were 65 300 730 reads and 66 603 686 reads transcriptome data were obtained from the peach fruits used bagging or not, and 85.73% and 84.60% reads could be compared to the peach reference genome sequence, respectively. Compared the transcriptome of shading bagged fruits with CK, 1 963 differentially expressed genes were found in the shading bagged fruits, including 1 255 up-regulated genes and 708 down-regulated genes. Annotation analysis indicated that 1 957 genes were annotated in Nr data, including 1 252 up-regulated genes and 705 down-regulated genes. Through COG analysis, there were 853 functional annotations of these differentially expressed genes, involving 23 functional classifications. With GO function annotation classifications, a total of 1 609 genes were divided into 53 function categories, in which many functional categories were mainly involved, such as molecular binding, catalytic activity, cell process, biological regulation. KEGG analysis showed that a total of 421 genes were annotated to 94 metabolic pathways, and photosynthesis signaling pathway, carbon fixation in photosynthetic organisms, photosynthesis-antenna proteins signaling pathway, flavonoid biosynthesis, ribosome biosynthesis (DNA replication) pathway were significantly enriched. The photosynthesis and flavonoid biosynthesis signaling pathway played an important role in fruit color, but the role of ribosome biosynthesis was not clear. The fruit quality also was detected from the shading bagged fruits and CK. The results showed that shading bagged peach fruits had a remarkable impact on soluble solids and total soluble sugar, which were significantly reduced. But the study also found, on average, little or no effect on total acid and per fruit weight from both the shading fruits and CK. 【Conclusion】 The number of differentially expressed genes under different physiological status of the fruit tissue in peach were obtained. The study found that the photosynthesis and flavonoid biosynthesis signaling pathway played an important role in fruit color and shading bagged peach fruits had a remarkable impact on soluble solids and total soluble sugar.

Key words: peach, ')">shading, bagging')">bagging, transcriptome, high-throughput sequencing, functional classification

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