24-表油菜素内酯对盐碱胁迫下大豆生育、生理及细胞超微结构的影响

doi:10.3864/j.issn.0578-1752.2017.05.004

Abstract

Abstract: 【Objective】The aim of this experiment was to study the effects of exogenous EBR (24-epibrassinolide) on the growth indices, physiological characteristics and cellular ultrastructure of soybean under saline-alkali stress. 【Method】Soybean variety Heinong 44 was employed as the test material, and cultivated in 110 mmol·L^-1nutrient solution saline-alkali stress treatments for 3 d and 7 d to investigate the effects of 1.2 mg·L^-1exogenous EBR on the height and root growth, SOD, POD, and APX three kinds of antioxidant enzyme activities, relative electrical conductivity, superoxide anion (O₂^-) production rate, contents of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA), free proline concentration content, chlorophyll content of soybean leaves and the responses of cellular ultrastructure of soybean leaves and root tips under saline-alkali stress. 【Result】Under the condition of saline-alkali stress dealing with 3 d and 7 d, compared to the control, the three kinds of antioxidant enzyme activities of SOD, POD, and APX, free proline content, relative electrical conductivity, O₂^-production rate, contents of H₂O₂ and MDA all increased, the growth indices and chlorophyll content all decreased; the chloroplast and mitochondria cellular ultrastructure of soybean leaves suffered severe damages. The mitochondria and endoplasmic reticulum cellular ultrastructure of soybean root tips suffered severe damages, and vacuole was ruptured. Application of EBR under saline-alkali stress increased soybean plant height, root length, fresh mass of root by 6.45%, 9.60% and 19.85%, respectively. Application of EBR the activities of SOD, POD, and APX of soybean leaves in 3 d and 7 d treatments increased by 16.92% and 9.68%, 48.85% and 61.44%, 19.05% and 20.36%, respectively, relative electrical conductivity, O₂^-production rate, H₂O₂ and MDA contents significantly decreased by 19.58% and 28.26%, 28.06% and 40.92%, 28.62% and 31.21%, 31.03% and 37.17%, respectively, free proline content and chlorophyll content significantly increased by 3.67% and 15.96%, 13.34% and 16.87%, respectively. At the same time, the stability of soybean leaves and root tip cell ultrastructure were maintained and the aging of cells and disintegration were delayed.【Conclusion】The results suggested that application of exogenous EBR under saline-alkali stress could increase the antioxidant enzyme activities, free proline concentration and chlorophyll content, reduce the accumulation of reactive oxygen species(ROS), maintain the cell structure integrity, promote seedling growth and resulting in improving the tolerance of soybean seedlings to saline-alkali stress.

Key words: soybean, saline-alkali stress, 24-epibrassinolide, fertility, physiological characteristics, cell ultra-structure

WU Yang, GAO HuiChun, ZHANG BiXian, ZHANG HaiLing, WANG QuanWei, LIU XinLei, LUAN XiaoYan, MA YanSong. Effects of 24-Brassinolide on the Fertility, Physiological Characteristics and Cell Ultra-Structure of Soybean Under Saline-Alkali Stress[J].Scientia Agricultura Sinica, 2017, 50(5): 811-821.

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Effects of 24-Brassinolide on the Fertility, Physiological Characteristics and Cell Ultra-Structure of Soybean Under Saline-Alkali Stress

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