不同磷水平下4种柑橘砧木的生长状况、根系形态和生理特性

doi:10.3864/j.issn.0578-1752.2015.03.13

Abstract

Abstract: 【Objective】Effects of different phosphorus (P) levels on the plants growth, roots morphology and related physiological characteristics of citrus rootstocks were studied for providing a scientific basis of high P efficiency citrus rootstocks excavation.【Method】The wild Citrus ichangensis Swingle, C. aurantium L., C. limonia Osbeck and cultivated species Poncirus trifoliata Raf. in Guizhou Karst mountain were used as materials, and the date including the growth and biomass, root morphological characteristics, activity of acid phosphatase (APase) and nitrate reductase (NR) of leaves and roots, and secretory acid phosphatase (SAPase) of roots and root absorption kinetics of P characteristics were researched through sand cultivation.【Result】The growth and development of C. ichangensis Swingle was less inhibited under 5 mg•L^-1 P level, followed by C. limonia Osbeck and C. aurantium L., restrained intensively to P. trifoliate Raf.. When P concentrations of nutrient solution was 25 mg•L^-1, the amount including the plant height, base diameter and fresh weight and dry weight of biomass, total root length, total surface area, total volume and total root tip reached the maximum for C. ichangensis Swingle and C. limonia Osbeck, followed by 5 mg•L^-1 P level, 45 mg•L^-1 P level was the minimum. With the P concentrations of nutrient solution increasing, the data including the plant height, base diameter and fresh weight and dry weight of biomass, total root length, total surface area, total volume, total root tip amount and root-shoot ratio of C. aurantium L. and P. trifoliata Raf. increased, however, the root-shoot ratio of C. ichangensis Swingle and C. limonia Osbeck decreased. Under low P conditions, C. ichangensis Swingle had the biggest lateral root roughness and the second lateral roots amount and root-shoot ratio, the NR activity in leaves was all in the order of C. ichangensis Swingle ＞C. limonia Osbeck ＞C. aurantium L. ＞P. trifoliata Raf., the NR activity in roots was all in the order of C. aurantium L. ＞C. limonia Osbeck ＞ C. ichangensis Swingle＞P. trifoliata Raf.. The NR activity in leaves and roots of different citrus rootstocks receded with P levels decreased significantly. The APase activity in leaves and roots, and the SAPase activity in roots was all in the order of C. ichangensis Swingle＞C. limonia Osbeck＞C. aurantium L. ＞P. trifoliata Raf. under low P stress. The I_max of C. ichangensis Swingle was greater than C. limonia Osbeck , C.aurantium L. and P. trifoliata Raf. under low P conditions, while the K_m and C_min was smaller than C. limonia Osbeck, C. aurantium L. and P. trifoliata Raf.【Conclusion】The ability to endure to low P stress of C. ichangensis Swingle was much stronger, and response of root morphology and physiological adaptation mechanisms were more obvious than the other three kinds of citrus rootstocks under low P conditions, which showed strong absorption characteristics of P dynamics.

Key words: citrus rootstock, phosphorus, growth, root morphology, physiological characteristics

FAN Wei-guo, LUO Yan. Growth Status, Root Morphology andPhysiologicalCharacteristics of Four Citrus Rootstocks Under Different Phosphorus Levels[J].Scientia Agricultura Sinica, 2015, 48(3): 534-545.

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References

[1] Carroll P V, Claudia U S，Deborah L A. Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. New Phytologist, 2003, 157(3): 423-447.

[2] Liao H, Yan X L. Adaptive changes and genotypic variation for root architecture of common bean in response to phosphorus deficiency. Acta Botanica Sinica, 2000, 42(2): 158-163.

[3] 陈磊, 王盛锋, 刘自飞, 刘荣乐, 汪洪. 低磷条件下植物根系形态反应及其调控机制. 中国土壤与肥料, 2011(6): 1-12.

Chen L, Wang S F, Liu Z F, Liu R L, Wang H. Morphological and physiological responses of plant root growth to low phosphorus stress. Soil and Fertilizer in China, 2011(6): 1-12. (in Chinese)

[4] 唐宏亮, 申建波, 张福锁, Zed R. 磷和外源生长素对白羽扇豆(Lupinus albus L.)根形态和生理特性的影响. 中国科学: 生命科学, 2013, 43(3): 201- 212.

Tang H L, Shen J B, Zhang F S, Zed R. Interactive effects of phosphorus deficiency and exogenous auxin on root morphological and physiological traits in white lupin (Lupinus albus L.). Scientia Sinica Vitae, 2013, 43(3): 201- 212. (in Chinese)

[5] Saber K, Ahmed D, Tarek S, Nahla L, Jean J D, Chedly A. Root proliferation, proton efflux, and acid phosphatase activity in common bean (Phaseolus vulgaris) under phosphorus shortage. Plant Biology, 2009, 52: 395-402.

[6] Kanehiro K. The activities of soil and root acid phosphatase in the nine tropical rain forests that differ in phosphorus availability on Mount Kinabalu, Borneo. Plant Soil, 2013, 367: 215-224.

[7] Nielsen N E, Barber S A. Differences among genotypes of corn in the kinetics of P uptake. Agronomy Journal, 1978, 70: 695-698.

[8] 谢钰容, 周志春, 金国庆, 陈跃. 马尾松不同种源P素吸收动力学特征. 林业科学研究, 2003, 16(5): 548-553.

Xie Y R, Zhou Z C, Jin G Q, Chen Y. P element absorption kinetics characteristics of different provenance mason pine. Forest Research, 2003, 16(5): 548-553. (in Chinese)

[9] 王孝纯, 周建朝, 於丽华, 肖东旭. 不同基因型甜菜无机磷吸收动力学参数研究. 中国甜菜糖业, 2007(2): 5-7.

Wang X C, Zhou J C, Yu L H, Xiao D X. Studies on kinetic parameters of inorganic phosphorus uptake in different sugar beet genotypes. China Sugar Beet Sugar, 2007(2): 5-7. (in Chinese)

[10] 蔡树美, 刘文桃, 张震, 柏彦超, 钱晓晴. 不同品种浮萍磷素吸收动力学特征. 生态与农村环境学报, 2011, 27(2): 48-52.

Cai S W, Liu W T, Zhang Z, Bai Y C, Qiang X Q. Phosphorus uptake kinetics of different types of duckweed. Journal of Ecology and Rural Environment, 2011, 27(2): 48-52. (in Chinese)

[11] 罗燕, 樊卫国. 不同施磷水平下4种柑橘砧木的根际土壤有机酸、微生物及酶活性. 中国农业科学, 2014, 47(5): 955-967.

LuoY, Fan W G. Organic acid content, microbial quantity and enzyme activity in rhizosphere soil of four citrus rootstocks under different phosphorus levels. Scientia Agricultura Sinica, 2014, 47(5): 955-967. (in Chinese)

[12] 申建波, 毛达如. 植物营养研究方法. 北京: 中国农业大学出版社, 2011: 12-20.

Shen J B, Mao D R. Plant Nutrition Research Methods. Beijing: China Agricultural University Press, 2011: 12-20. (in Chinese)

[13] Lopez-Bucio J, Hernandez-Abreu E, Sanchez-Calderon L, Nieto-Jacobo M F, Simpson J, Herrera-Estrella L. Phosphate availability alters architecture and causes changes in hormone sensitivity in the Arabidopsis root system. Plant Physiology, 2002, 129: 244-256.

[14] 张志良, 瞿伟菁. 植物生理学实验指导. 第3版. 北京: 高等教育出版社, 2003: 25-44.

Zhang Z L, Qu W J. The Guide of Plant Physiological Experiment. 3rd ed.. Beijing: Higher Education Press, 2003: 25-44. (in Chinese)

[15] 林启美, 黄德明. 应用酸性磷酸酶进行番茄磷素诊断. 华北农学报, 1991, 6(2): 73-83.

Lin Q M, Huang D M. Evaluation of an acid phosphatase assay for detection of phosphorus deficiency in tomato leaves. Acta Agriculturae Boreali-Sinica, 1991, 6(2): 73-83. (in Chinese)

[16] 樊明寿, 徐冰, 王艳. 缺磷条件下玉米根系酸性磷酸酶活性的变化. 中国农业科技导报, 2001, 3(3): 33-36.

Fan M S, Xu B, Wang Y. Acid phosphatase activities of intact roots and ground root tissues of maize grown in high P or low P nutrient solution. Journal of Agricultural Science and Technology, 2001, 3(3): 33-36. (in Chinese)

[17] 蒋廷惠, 郑绍建, 石锦芹, 胡霭堂, 史瑞和, 徐茂. 植物吸收养分动力学研究中的几个问题. 植物营养与肥料学报, 1995, 1(2): 11-17.

Jiang H T, Zheng S J, Shi J Q, Hu A T, Shi R H, Xu M. Serveral considerations in kinetic research on nutrients uptake by plants. Plant Nutrition and Fertilizer Science, 1995, 1(2): 11-17. (in Chinese)

[18] 鲍士旦. 土壤农化分析. 北京: 中国农业出版社, 2000: 71-73.

Bao S D. Soil Agrochemistry Analysis. Beijing: China Agricultural Press, 2000: 71-73. (in Chinese)

[19] 严小龙, 廖红, 戈振扬, 罗锡文. 植物根构型特性与磷吸收效率. 植物学通报, 2000, 17(6): 511-519.

Yan X L, Liao H, Ge Z Y, Luo X W. Root architectural characteristics and phosphorus acqtfisifion eficiency in plants. Chinese Bulletin of Botany, 2000, 17(6): 511-519. (in Chinese)

[20] 张福锁, 申建波, 冯固. 根际生态学. 北京: 中国农业大学出版社, 2009.

Zhang F S, Shen J B, Feng G. Rhizosphere Ecology. Beijing: China Agricultural University Press, 2009. (in Chinese)

[21] Ericsson T. Growth and shoot-root ratio of seedlings in relation to nutrient availability. Plant and Soil, 1995, 168: 205-214.

[22] Nielsen K L, Eshel A, Lynch J P. The effect of phosphorus availability on the carbon economy of contrasting common bean (Phaseolus vulgaris L.) genotypes. Journal of Experiental Botany, 2001, 52: 329-339.

[23] 何鹏, 王大鹏, 韦家少, 吴敏, 吴炳孙, 高乐, 覃怀德. 低磷胁迫对巴西橡胶树幼苗若干生理代谢指标的影响. 热带作物学报, 2012, 33(11): 1976-1979. (in Chinese)

He P, Wang D P, Wei J S, Wu M, Wu B S, Gao L, Qin H D. Low phoshorus stress on the physiological metabolism of hevea seedling. Chinese Journal of Tropical Crops, 2012, 33(11): 1976-1979. (in Chinese)

[24] 李景娟, 高建伟. 氮磷调控根系形态的分子机制研究进展. 山东农业科学, 2013, 45(6): 123-129.

Li J J, Gao J W. Research advances in molecular regulation mechanism of nitrogen and phosphorus on root morphology. Shandong Agricultural Science, 2013, 45(6): 123-129. (in Chinese)

[25] Gaume A, Machler F, Leon C D, Narro L, Frossard E. Low-P tolerance by maize (Zea mays L.) genotypes: significance of root growth, and organic acids and acid phosphatase root exudation. Plant and Soil, 2001, 228: 253-264.

[26] Lim J H, Chung I M, Ryu S S, Park M R, Song J Y. Differential responses of rice acid phosphatase activities and isoforms to phosphorus deprivation. Journal of Biochemistry and Molecular Biology, 2003, 36(6): 597-602.

[27] 黄宇, 张海伟, 徐芳森. 植物酸性磷酸酶的研究进展. 华中农业大学学报, 2008, 27(1): 148-154.

Huang Y, Zhang H W, Xu F S. Research summarization on acid phosphatase in higher plants. Journal of Huazhong Agricultural University, 2008, 27(1): 148-154. (in Chinese)

[28] Fernando C B Z, Dirceu M, Pedro R F, José A Q, Rodrigo M B. Efficiency of phosphorus uptake and utilization in citrus rootstocks. Revista Brasileira de Ciência, 2012, 36(2): 485-496.

[29] 李天忠, 张志宏. 现代果树生物学. 北京: 科学出版社, 2008: 290-295.

Li T Z, Zhang Z H . Modern Fruit Biology. Beijing: Science Press, 2008: 290-295. (in Chinese)

[30] 陆景陵. 植物营养学. 北京: 北京农业出版社, 1994: 28-33.

Lu J L. Plant Nutrition. Beijing: Beijing Agricultural University Press, 1994: 28-33. (in Chinese)

[31] 齐炳林, 曹翠玲, 王菲, 雷忠萍, 赵倩茹, 李君. 磷胁迫对豇豆幼苗硝酸还原酶活性和硝态氮含量的影响. 干旱地区农业研究, 2010, 28(1): 147-151.

Qi B L, Cao C L, Wang F , Lei Z P, Zhao Q R, Li J. Influence of low phosphorus on nitrate reductive activity and NO₃^--N content in cowpea seedling. Agricultural Research in the Arid Areas, 2010, 28(1): 147-151. (in Chinese)

[32] 蔡柏岩, 葛菁萍, 祖伟. 施磷水平对不同基因型大豆品种硝酸还原酶活性影响. 大豆科学, 2007, 26(3): 359-362.

Cai B Y, Ge J P, Zu W. The affection of phosphours level to different genotype soybean nitrate reduce tase (NR) activities. Soybean Science, 2007, 26(3): 359-362. (in Chinese)

[33] 吴明才, 肖昌珍, 郑普英. 大豆磷营养研究. 中国农业科学, 1999, 32(3): 59-65.

Wu M C, Xiao C Z, Zheng P Y. Study on the physiological function of phosphorus to soybean. Scientia Agricultura Sinica, 1999, 32(3): 59-65. (in Chinese)

[34] 乔海涛, 杨洪强, 范伟国. 缺素环境中平邑甜茶根系硝酸还原酶活性及硝态氮含量的变化. 中国农学通报, 2007, 23(10): 114-117.

Qiao H T, Yang H Q, Fan W G. Changes of nitrate reductase activity and nitrate nitrgen content in the root of Malus hupehensis Rehd under element deficient condition. Chinese Agricultural Science Bulletin, 2007, 23(10): 114-117. (in Chinese)

[35] 樊卫国, 安华明, 刘国琴. 养分和水分状况对刺梨树体硝酸还原酶活性与氮素营养的影响. 果树学报, 2007, 24(2): 162-167.

Fan W G, An H M, Liu G Q. Effects of nutrition condition and drought stress on NR activity and nitrogen nutrition in organs of Rosa roxburghii. Journal of Fruit Science, 2007, 24(2): 162-167. (in Chinese)

[36] 樊卫国, 王立新. 不同供磷水平对纽荷尔脐橙幼树生长及叶片营养元素含量的影响. 中国农业科学, 2012, 45(4): 714-725.

Fan W G, Wang L X. Effect of different phosphorus levels on growth and leaf element contents of young Newhall navel orange trees. Scientia Agricultura Sinica, 2012, 45(4): 714-725. (in Chinese)

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