Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (23): 4977-4984.doi: 10.3864/j.issn.0578-1752.2013.23.014

• HORTICULTURE • Previous Articles     Next Articles

Diversity of Resistance to Jujube Witches’ Broom Disease Among the Strains of Ziziphus jujuba Mill. Junzao

 XIAO  Jing-1, YANG  Yan-Rong-1, ZHAO  Jin-2, LIU  Meng-Jun-1   

  1. 1.Research Center of Chinese Jujube, Agricultural University of Hebei, Baoding 071001, Hebei
    2.College of Life Science, Agricultural University of Hebei, Baoding 071000, Hebei
  • Received:2013-08-05 Online:2013-12-01 Published:2013-09-27

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Abstract: 【Objective】Sixteen strains of Z. jujuba Junzao were collected and evaluated, aiming at revealing the diversity of resistance to JWB within cultivar, screening excellent strains with high resistance. 【Method】The JWB symptom development and phytoplasma infection were studied through field investigation and PCR analysis. The fruit economical characters, including the rate of water content, fruit weight, fruit shape index, ratio of edibility, sugar content, Vc content and titratable acidity, were comprehensively evaluated among different strains using synthesis-reasonable satisfaction and multivalue theory merging rule. The genetic diversity of Junzao strains were analyzed by AFLP. 【Result】 Different strains of Junzao showed significant differences in JWB resistance. According to the situations of disease symptom development and phytoplasma infection, the strains were divided into four groups: sensitive, delayed sensitive, delayed resistant and resistant, and 4 strains of Junzao (T15, J2, J5, J8) with very high resistance to JWB were screened out. In the resistant group, the fruit quality of T15, J5 and J8 was also at higher level among the sixteen strains. AFLP analysis showed that there had significant differences in DNA level among different strains of Junzao. 【Conclusion】 Four strains of Junzao (T15, J2, J5, J8) with very high resistance to JWB were screened out, and three of them (T15, J5 and J8) also have an excellent fruit quality.

Key words: Junzao , jujube witches&rsquo, broom , symptom investigation , phytoplasma , fruit quality , resistant strains

[1]刘孟军, 赵锦, 周俊义. 枣疯病. 北京: 中国农业出版社, 2010: 12-14.

Liu M J, Zhao J, Zhou J Y. Jujube Witches’ Broom Disease. Beijing: China Agriculture Press, 2010: 12-14. (in Chinese)

[2]赵锦, 刘孟军. 抗枣疯病种质资源的筛选与应用. 植物遗传资源学报, 2006, 7(4): 398-403.

Zhao J, Liu M J. Screening and application of Chinese jujube germplasm with high resistance to withes’ broom disease. Journal of Plant Genetic Resources, 2006, 7(4): 398-403. (in Chinese)

[3]田国忠, 李志清, 赵俊芳, 谢守江, 胡佳续, 赵京芳, 王振亮, 史忠儒, 温秀军, 张志善. 我国部分枣树品种（系）对枣疯病的抗性研究//第三届中国森林保护学术大会论文摘要集. 2010: 25.

Tian G Z, Li Z Q, Zhao J F, Xie S J, Hu J X, Zhao J F, Wang Z L, Shi Z R, Wen X J, Zhang Z S. Some Chinese jujube varieties (strains) on the resistance of JWB//Third China Forest Protection Academic Conference Abstract Book. 2010: 25. (in Chinese)

[4]温秀军, 孙朝晖, 孙士学, 史忠儒, 陈德廷. 抗枣疯病枣树品种及品系的选择. 林业科学, 2001, 37(5): 87-92.

Wen X J, Sun C H, Sun S X, Shi Z R, Chen D T. Selection of resistant varieties and individuals of jujube trees to jujube witches’ broom disease. Scientia Silvae Sinicae, 2001, 37(5): 87-92. (in Chinese)

[5]温秀军, 郭晓军, 田国忠, 孙朝晖, 李永. 几个枣树品种和婆枣单株对枣疯病抗性的鉴定. 林业科学, 2005, 41(3): 88-96.

Wen X J, Guo X J, Tian G Z, Sun C H, Li Y. Identification of resistances of several jujube cultivars and selected Pozao single trees against jujube witches’ broom disease. Scientia Silvae Sinicae, 2005, 41(3): 88-96. (in Chinese)

[6]温秀军, 孙朝晖, 田国忠, 郭小军, 王振亮, 李永. 抗枣疯病枣树品系选育及抗病机理初探. 林业科技开发, 2006, 20(5): 12-18.

Wen X J, Sun C H, Tian G Z, Guo X J, Wang Z L, Li Y. A preliminary study on the disease-resistant mechanism and selection of varieties in jujube witches’ broom. China Forestry Science and Technology, 2006, 20(5): 12-18. (in Chinese)

[7]刘孟军, 赵锦, 周俊义. 枣疯病病情分级体系研究. 河北农业大学学报, 2006, 29(1): 31-33.

Liu M J, Zhao J, Zhou J Y. Grading system of jujube witches’ broom symptom. Journal of Agricultural University of Hebei,  2006, 29(1): 31-33. (in Chinese)

[8]李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000.

Li H S. The Principle and Technology of Plant Physiology and Biochemistry Experiment. Beijing: Higher Education Press, 2000. (in Chinese)

[9]王浣尘. 多维价值理论在工业技术系统选择和设计中的应用. 国外自动化, 1982(1): 11-21.

Wang H C. Application of multi-dimensional value theory in the selection and design of the industrial technology system. Foreign Automation, 1982(1): 11-21. (in Chinese)

[10]贺仲雄. 模糊数学与应用. 天津: 天津科学技术出版社, 1983.

He Z X. Fuzzy Mathematics and Application. Tianjin: Tianjin Science and Technology Press, 1983. (in Chinese)

[11]赵思东, 胡春水, 田伟政, 杜正涛, 李前华. 湖南南部柑桔栽培品种评价及调整研究. 经济林研究, 1998(3): 8-11.

Zhao S D, Hu C S, Tian W Z, Du Z T, Li Q H. Evaluation and adjustment of citrus cultivars of the southern Hunan. Economic Forest Researches, 1998(3): 8-11. (in Chinese)

[12]何照范, 张迪请. 保健食品化学及其检测技术. 北京: 中国轻工业出版社, 1999: 99-100.

He Z F, Zhang D Q. Health Food Chemistry and Its Detection Technology. Beijing: China Light Industry Press, 1999: 99-100. (in Chinese)

[13]无锡工业大学,天津轻工业学院. 食品分析. 北京: 中国轻工业出版社, 2001: 104.

Industrial University of Wuxi, Light Industry College of Tianjin. Food Analysis. Beijing: China Light Industry Press, 2001: 104. (in Chinese)

[14]Sambrook J, Bussell D W. Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harber Laboratory Press, 2001: 898-915.

[15]乔勇, 赵锦, 杨海旭, 刘孟军. 21个枣品种(系)的AFLP指纹分析. 植物遗传资源学报, 2009, 10(2): 205-210.

Qiao Y, Zhao J, Yang H X, Liu M J. AFLP analysis on 21 cultivars and strains of Chinese jujube. Journal of Plant Genetic Resources, 2009, 10(2): 205-210. (in Chinese)

[16]Sarwat M, Das S, Srivastava P S. Analysis of genetic diversity through AFLP, SAMPL, ISSR and RAPD markers in Tribulus terrestris, a medicinal herb. Plant Cell and Reports, 2008, 27: 519-528.

[17]Russi L, Moretti C, Raggi L, Albertini E, Falistocco E. Identifying commercially relevant Echinacea species by AFLP molecular markers. Genome, 2009, 52: 912-918.

[18]Tripathi N, Saini N, Tiwari S. Assessment of genetic diversity among Aloe vera accessions using amplified fragment length polymorphism. International Journal of Medicinal and Aromatic Plants, 2011, 1: 115-121.

[19]Misra A, Shasany A K, Shukla A K, Darokar M P, Singh S C, Sundaresan V, Singh J, Bagchi G D, Jain S P, Saikia D, Khanuja S P S. AFLP markers for identification of Swertia species (Gentianaceae). Genetic and Molecular Research, 2010, 9: 1535-1544.

[20]Shu Z, Wang Z, Mu X, Liang Z, Guo H. A dominant gene for male sterility in Salvia miltiorrhiza Bunge. PLoS One, 2012, 7(11): e50903.

[21]Siosemardeh A, Osmani Z, Bahramnejad B, Vahabi K, Roohi E. Identification of AFLP markers associated with stress tolerance index in Sardari wheat ecotypes. Journa of Agricultural Science and Technology, 2012, 14: 629-643.

[22]Liu Z W, Fu T D, Tu J X, Chen B Y. Inheritance of seed color and identification of RAPD and AFLP markers linked to the seed in rapeseed (Brassica napus L.). Theoretical and Applied Genetics, 2005, 110: 303-310.

[23]Negi M S, Devic M, Delseny M, Lakshmikumaran M. Identification of AFLP fragments linked to seed coat colour in Brassica junca and conversion to a SCAR marker for rapid selection. Theoretical and Applied Genetics, 2009, 101: 146-152.

[24]黄河, 王顺利, 戴思兰. 利用cDNA-AFLP技术鉴定菊花品种‘紫荷’的抗白锈病相关基因. 中国农业科学, 2012, 45(5): 926-935.

Huang H, Wang S L, Dai S L. cDNA-AFLP analysis of white rust response genes in Chrysanthemum morifolium ‘Zihe’. Scientia Agricultura Sinica, 2012, 45(5): 926-935. (in Chinese)

[25]Zhang Y, Guo L, Shu Z, Sun Y, Chen Y, Liang Z and Guo H. Identification of amplified fragment length polymorphism (AFLP) markers tightly associated with drought stress gene in male sterile and fertile Salvia miltiorrhiza Bunge. International Journal of Molecular Sciences, 2013, 14: 6518-6528.
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