水稻籼粳交F8、F2群体穗长QTL比较分析

doi:10.3864/j.issn.0578-1752.2013.23.001

Abstract

Abstract: 【Objective】In this study, rice panicle length QTL was analyzed by using F8 RIL population, the similarities and differences of the genetic map, QTL locations, QTL effects in different derivative population of the same parent were compared and the stable expression of QTL of panicle length trait was identified. The results of the study will provide more information about the genetic behavior of rice panicle length and be benefit to the MAS (marker-assisted selection).【Method】A F8 population containing 188 lines, which was derived from the cross between the indica variety Luhui99 and the japonica variety Nipponbare, was analyzed with a genetic linkage map consisting 207 DNA markers. The statistic software of QTL Network 2.0 was applied to detect QTL and QTL effect analysis for rice panicle length. The similarities and differences of QTL mapping and genetic map between F8 and F2 population were discussed. 【Result】A total of 7 QTL with significant additive effects were detected on chromosomes 2, 3, 6, 7, 8 and 10 in F8 population. The proportions of phenotypic variance explained by individual QTL ranged from 3.38% to 14.8% and the QTL could jointly explain 52.5% of the total phenotypic variance. There were five same chromosomes having rice panicle length QTL in F8 and F2 genetic map and most of the QTL’s physical locations are overlapping or containment. The F8 and F2 genetic maps differed in molecular marker number, marker arrangement, genetic distance and average distance between the markers. 【Conclusion】 Seven rice panicle length QTL were detected in F8 RIL population. Although there were many differences betwen F8 and F2 populations in genetic map, QTL analysis and so on, a steady main QTL was found on the sixth chromosome. In addition, four stable rice panicle length QTL were detected in the study which were not reported in previous references.

Key words: rice , panicle length , QTL mapping

GUO Xiao-Jiao-2, ZHANG Tao-13, JIANG Kai-Feng-13, YANG Li-13, CAO Ying-Jiang-1, YANG Qian-Hua-1, YOU Shu-Mei-1, WAN Xian-Qi-3, LUO Jing-1, LI Zhao-Xiang-1, GAO Lei-1, ZHENG Jia-Kui-123. Comparison of Panicle Length QTL Based on F2 and F8 Populations Derived from Rice Subspecies Cross[J].Scientia Agricultura Sinica, 2013, 46(23): 4849-4857.

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Comparison of Panicle Length QTL Based on F2 and F8 Populations Derived from Rice Subspecies Cross

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