Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (19): 4101-4109.doi: 10.3864/j.issn.0578-1752.2013.19.016

• HORTICULTURE • Previous Articles     Next Articles

Analysis of Organic Acid Accumulation Characteristics and Organic Acid-Metabolizing Enzyme Activities of Chinese Dwarf Cherry (Cerasus humilis Bunge) Fruit

 WANG  Peng-Fei-1, XUE  Xiao-Fang-12, MU  Xiao-Peng-1, ZHANG  Jian-Cheng-1, CAO  Qin-1, DU  Jun-Jie-1   

  1. 1.College of Horticulture, Shanxi Agricultural University, Taigu 030801, Shanxi
    2.Research Institute of Fruit Tree，Shanxi Academy of Agricultural Sciences, Taigu 030801, Shanxi
  • Received:2013-05-13 Online:2013-10-01 Published:2013-07-04

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Abstract: 【Objective】 The accumulation characteristics of main organic acid and activities of acid-related enzymes between two cultivars of Chinese dwarf cherry with different acidities were compared. 【Method】 Using the method of ultra performance liquid chromatograph (UPLC), the contents of total organic acids, malic acid and citric acid, as well as the activities of organic acid-metabolizing enzymes were analyzed during the fruit development of 6-year-old Chinese dwarf cherry ‘Nongda 3’ and ‘Nongda 4’. 【Result】Malic acid and citric acid of these two varieties accumulated mainly in the late period of fruit development, but their accumulation rates were quite different. Because of the high NADP-ME activity and the low NAD-MDH and PEPC activities, there was only a small amount of malic acid accumulation in the early period of fruit development. During the late period, NADP-ME activity decreased rapidly and NAD-MDH activity and PEPC activity began increasing, which promoted a massive accumulation of malic acid. The sudden increase of NADP-ME activity and the decline of NAD-MDH activity and PEPC activity caused the degradation of mailc acid at the ripening stage. Before the ripening stage(18-19 weeks after anthesis), NAD-MDH activity of ‘Nongda 4’ was higher than the activity of ‘Nongda 3’ , while NADP-ME activity of ‘Nongda 4’ was lower. As a result, the content of malic acid of ‘Nongda 4’ was higher than the content of ‘Nongda 3’. The content of citric acid of ‘Nongda 3’ was higher (17-19 weeks after anthesis) because its CS activity was higher at the ripening stage.【Conclusion】The late period of fruit development is a critical period for the accumulation of malic acid and citric acid. The difference in malic acid accumulation is mainly caused by the coordination change of NAD-MDH activity and NADP-ME activity, while the difference in the accumulation of citric acid is mainly affected by the change of CS activity.

Key words: Chinese dwarf cherry , fruit , malic acid , citric acid , metabolism-related enzymes

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