Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (14): 3046-3054.doi: 10.3864/j.issn.0578-1752.2013.14.021

• RESEARCH NOTES • Previous Articles     Next Articles

Differentially Expressed Protein Profile of Maize Seedling Leaves Between Hybrid and Its Parental Lines

 GUO  Bao-Jian, SUI  Zhi-Peng, LI  Yang-Yang, FENG  Wan-Jun, YAN  Wen-Wen, LI  Hui-Min, SUN  Qi-Xin, NI  Zhong-Fu   

  1. State Key Laboratory for Agrobiotechnology, China Agricultural University / National Plant Gene Research Centre (Beijing)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education / Beijing Key Laboratory of Crop Genetic Improvement, Beijing 100193
  • Received:2013-02-21 Online:2013-07-15 Published:2013-04-16

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Abstract: 【Objective】To construct protein expression profile of seedling leaves between maize hybrid and its parental lines, with the purpose to give an insight into the molecular basis of leaf size heterosis. 【Method】 Differentially expressed protein profile of the third leaf on 5th day after germination between maize hybrid and its parental lines used for 2-DE analysis and differentially expressed proteins were identified by using MALDI TOF MS method. 【Result】 A total of 630 protein spots were detected, among which 52 protein spots were found to be differentially expressed between maize hybrid and its parental lines. Differentially expressed protein spots could be grouped into six models, that is UPF1 (expression in hybrid and uniparent but not in another parent), HDH(hybrid is equal to the highly expressed parent), LDH (hybrid is equal to the lowly expressed parent), URH (up-regulated in hybrid), DRH (down-regulated in hybrid), F1nBP (hybrid-specific expressed protein spots), and the numbers were 15, 13, 8, 6, 7 and 3, respectively. Moreover, 28 of the 52 differentially expressed protein spots were identified by using MALDI TOF MS, which were grouped into nine functional categories, including metabolism, development, stress, glycolysis, transcription regulation, protein folding and degradation, tricarboxylic acid cycle, cytoskeleton and unknown protein. 【Conclusion】 Significant alterations in protein expression occurred in seedling leaves of hybrids and parental lines and differentially expressed proteins implicated in some functional categories might contribute to heterosis related to leaf size heterosis.

Key words: maize , leaf size , heterosis , differentially expressed protein , molecular mechanism

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