Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (11): 2394-2402.doi: 10.3864/j.issn.0578-1752.2013.11.025

• RESEARCH NOTES • Previous Articles    

Genetic Diversity of Large-Flowered Chrysanthemum Cultivars Revealed by ISSR Analysis

 LUO  Xin-Yan, WANG  Chen, DAI  Si-Lan, LI  Bao-Qin, LIU  Qian-Qian, ZHU  Jun, LU  Jie   

  1. College of Landscape, Beijing Forestry University/National Flower Engineering Research Center, Beijing 100083
  • Received:2012-06-27 Online:2013-06-01 Published:2013-04-11

PDF

788

Cited

3

Abstract: 【Objective】 Large-flowered chrysanthemum processes numerous cultivars with complex morphological types. This study aims to facilitate the research on classification and evolution history of chrysanthemum cultivars by a probe in genetic diversity of chrysanthemum cultivars belonging to different floral types respectively.【Method】 The genetic diversity of 150 large-flowered chrysanthemum cultivars belonging to 5 floral types were investigated using 10 primers with clear bands and steady repetitiveness chosen from 50 ISSR primers. 【Result】 A total of 229 bands were amplified, of which 220 were polymorphic loci. The main index were: PPB＝96.07%, He＝0.3485, I＝0.5154(at species level). The genetic variation in each floral types is quite high, in which the Flat-petal group is the highest (PPB＝95.20%), whereas the Anemone-petal group (PPB＝86.90%) and the Spoon-petal group (PPB＝89.52%) were the lowest. The level of genetic differentiation among floral types was very low, and genetic diversity mainly derived from variations of cultivars at flower type level. The result of UPGMA cluster was highly relevant to morphological variations among floral types. 【Conclusion】 The result of the research shows that genetic diversity of large-flowered chrysanthemum cultivars can be revealed well and truly by ISSR molecular marker，which processes of great meaning to research of classification, identification and evolution of chrysanthemum cultivars.

Key words: large-flowered chrysanthemum , floral type , genetic diversity , ISSR

[1]戴思兰, 陈俊愉. 菊属7个种的人工种间杂交试验. 北京林业大学学报, 1996 (4): 16-22.

Dai S L, Chen J Y. Artificial interspecific cross among seven species of Dendranthema in China. Journal of Beijing University, 1996(4): 16-22. (in Chinese)

[2]李鸿渐, 邵建文. 中国菊花品种资源的调查收集和分类. 南京农业大学学报, 1990, 13(1): 30-36.

Li H J, Shao J W. Investigation, collection and classification of     chrysanthemum cultivars in China. Journal of Nanjing Agricultural University, 1990, 13(1): 30-36. (in Chinese)

[3]戴思兰, 陈俊愉, 李文彬. 菊花起源的RAPD分析. 植物学报, 1998, 40(11): 1053-1059.

Dai S L, Chen J Y, Li W B.  Application of RAPD analysis in the study on the origin of Chinese cultivated chrysanthemum, Acta Botanica Sinica, 1998, 40(11): 1053-1059. (in Chinese)

[4]戴思兰, 王文奎, 黄家平. 菊属系统学及菊花起源研究进展. 北京林业大学学报, 2002, 24(5/6): 230-234.

Dai S L, Wang W K, Huang J P. Advances of researches on phylogeny of Dendranthema and origin of chrysanthemum. Journal of Beijing Forestry University, 2002, 24(5/6): 230-234. (in Chinese)

[5]汤忠皓. 中国菊花品种分类的探讨. 园艺学报, 1963, 2: 411-420.

Tang Z H. Study on classification of Chinese chrysanthemum varieties. Acta Horticulturae Sinica, 1963, 2: 411-420. (in Chinese)

[6]张莉俊, 戴思兰. 菊花种质资源研究进展. 植物学报, 2009, 44(5): 526-535.

Zhang L J, Dai S L. Research advance on germplasm resource of Chrysanthemum×morifolium. Chinese Bulletin of Botany, 2009, 44(5): 526-535. (in Chinese)

[7]张树林. 菊花品种分类的研究. 园艺学报, 1965, 4(1): 30-36.

Zhang S L. Studies on the classification of garden varieties of florists’ chrysanthemum. Acta Horticulturae Sinica, 1965, 4(1): 30-36. (in Chinese)

[8]马克平. 试论生物多样性的概念. 生物多样性, 1993, 1(1): 20-22.

Ma K P. On the concept of biodiversity. Biodiversity Science, 1993, 1(1): 20-22. (in Chinese)

[9]Solbrig O T. From Genes to Ecosystems: A Research Agenda for Biodiversity. Cambridge, Mass.: IUBS, 1991.

[10]秦贺兰, 游捷, 高俊平. 菊花18个品种的RAPD分析. 园艺学报, 2002, 29(5): 488-490.

Qin H L, You J, Gao J P. RAPD analysis of 18 chrysanthemum cuitivars. Acta Horticulturae Sinica, 2002, 29(5): 488-490. (in Chinese)

[11]韩洁, 胡楠, 李玉阁, 尚富德. 菊花品种资源遗传多样性AFL分析. 园艺学报, 2007, 34(4): 1041-1046.

Han J, Hu N, Li Y G, Shang D F. Genetic diversity of chrysanthemum cultivars revealed by AFLP analysis. Acta Horticulturae Sinica, 2007, 34(4): 1041-1046. (in Chinese)

[12]田赟, 雒新艳, 戴思兰. RAPD技术在菊花芽变品种鉴定中的应用.分子植物育种, 2008, 6(6): 1223-1232.

Tian Y, Luo X Y, Dai S L. RAPD analysis of sporting and similar cultivars in Chrysanthemum. Molecular Plant Breeding, 2008, 6(6): 1223-1232. (in Chinese)

[13]邹喻萍,葛颂,王晓东. 系统与进化植物学中的分子标记. 北京: 科学出版社, 2001.

Zou Y P, Ge S, Wang X D. Molecular Markers in Systematic and Evolutionary Botany. Beijing: Science Press, 2001. (in Chinese)

[14]邱英雄, 胡绍庆, 陈跃磊, 陈晓, 吴光洪. ISSR-PCR技术在桂花品种分类中的应用. 园艺学报, 2004, 31(4): 529-532.

Qiu Y X, Hu S Q, Chen Y L, Chen X, Wu G H. Studies on cultivar classification of Osmanthus fragrans by ISSR-PCR analysis. Acta Horticulturae Sinica, 2004, 31(4): 529-532. (in Chinese)

[15]金则新, 李均敏, 顾奇萍.云锦杜鹃自然居群遗传多样性的ISSR分析. 园艺学报, 2006, 33(6): 1263-1267.

Jin Z X, Li J M, Gu Q P. Genetic diversity in the natural populations of Rhododendron fortunei revealed by ISSR molecular markers. Acta Horticulturae Sinica, 2006, 33(6): 1263-1267. (in Chinese)

[16]代红艳, 郭修武, 张叶, 李媛媛, 李贺, 周传生, 张志宏. 山楂(Crataegus pinnatifida Bge.)遗传多样性的RAPD和ISSR分析. 园艺学报, 2008, 35(8): 1117-1124.

Dai H Y, Guo X W, Zhang Y, Li Y Y, Li H, Zhou C S, Zhang Z H. Genetic diversity of Crataegus pinnatifida Bge. as evaluated by RAPD and ISSR markers. Acta Horticulturae Sinica, 2008, 35(8): 1117-1124. (in Chinese)

[17]周俐宏, 张金柱, 李振, 车代弟. ISSR揭示不同类型月季遗传多样性. 基因组学与应用生物学, 2009, 28(2): 311-315.

Zhou L H, Zhang J Z, Li Z, Che D D. Genetic diversity of different roses revealed by ISSR. Genomics and Applied Biology, 2009, 28(2): 311-315. (in Chinese)

[18]苏家乐, 姜维华, 冯立国, 李畅, 陶俊.部分一品红品种遗传关系的ISSR分析. 扬州大学学报 : 农业与生命科学版, 2010, 31(3): 86-89.

Su J L, Jiang W H, Feng L G, Li C, Tao J. ISSR analysis of genetic diversity among some cultivars of poinsettia (Euphorbia pulcherrima Willd.). Journal of Yangzhou University: Agricultural and Life Science Edition , 2010, 31(3): 86-89. (in Chinese)

[19]缪恒彬, 陈发棣, 赵宏波. 85个大菊品种遗传关系的ISSR分析. 园艺学报, 2007, 34(5): 1243-1248.

Miu H B, Chen F D, Zhao H B. Genetic relationship of 85 chrysanthemum  [Dendranthema × grandiflora (Ramat.) Kitamura]cultivars revealed by ISSR analysis. Acta Horticulturae Sinica, 2007, 34(5): 1243-1248. (in Chinese)

[20]Quinn G P, Keough M J. Experimental Design and Data Analysis for Biologists. Cambridge, UK: Cambridge University Press, 2003.

[21]Porebski S, Bailey L G, Baum B R. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Molecular Biology Reporter, 1997, 15(1): 8-15.

[22]Yeh F C, Yang R, Boyle T J, Ye Z, Xiyan M. POPGENE 32, Microsoft Windows-based Freeware for Population Genetic Analysis, version1.32. Edmonton, Canada. University of Alberta, 2000.

[23]Excoffier L, Smouse P, Quattro J M. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics, 1992, 131: 479-491.

[24]Rohlf F J. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Version 2.1. Exeter Software. New York, 2000.

[25]吴在生, 李海龙, 刘建辉, 左志锐, 田瑞昌. 65个菊花栽培品种遗传多样性的AFLP分析. 南京林业大学学报 :自然科学版, 2007, 31(5): 67-70.

Wu Z S, Li H L, Liu J H, Zuo Z R, Tian R C. Analysis of genetic diversity among 65 chrysanthemum cultivars based on AFLP. Journal of Nanjing Forestry University: Natural Science Edition, 2007, 31(5): 67-70. (in Chinese)

[26]刘民, 张世红, 梁海永, 甄志先.部分菊花品种遗传多样性的AFLP分析. 河北农业大学学报, 2008, 31(1): 48-52.

Liu M, Zhang S H, Liang H Y, Zhen Z X. AFLP analysis on the Genetic diversity of some chrysanthemum species. Journal of Agricultural University of Hebei, 2008, 32(1): 48-52. (in Chinese)

[27]欧阳彩虹, 何桥, 秦国新, 郭启高, 韩国辉, 王勇, 秦兵, 梁国鲁. 25个菊花品种遗传多样性的ISSR分析.山西农业大学学报:自然科学版, 2010, 30(3): 201-204.

Ouyang C H, He Q, Qin G X, Guo Q G, Han G H, Wang Y, Qin B, Liang G L. Genetic diversity of 25 chrysanthemum [Dendranthema × grandiflora (Ramat.) Kitamura]cuitivars revealed by ISSR analysis. Journal of Shandong Agriculture University: Natural Science Edition, 2010,  30(3): 201-204. (in Chinese)

[28]雒新艳, 刘倩倩, 白新祥, 朱珺, 卢洁, 李宝琴, 戴思兰. 北京地区独本菊品种资源及花型和花色分布研究. 北方园艺, 2009(7): 183-188.

Luo X Y, Liu Q Q, Bai X X, Zhu J, Lu J, Li B Q, Dai S L. Flower type and color distribution of single-stem chrysanthemum cultivars in Beijing area. Northern Horticulture, 2009(7): 183-188. (in Chinese)

[29]Nei M. Estimation of average heterozygosis and genetic distance from a small number of individuals. Genetics, 1978, 89(3): 583-590.

[30]Wright S. Evolution in Mendelian population. Genetics, 1931, 16: 97-159.

[31]刘春迎, 王莲英.菊花品种的数量分类研究. 北京林业大学学报, 1995, 17(2): 79-87.

Liu C Y, Wang L Y. The numerical classification of some cultivars of florist’s chrysanthemum. Journal of Beijing Forestry University, 1995, 17(2):79-86. (in Chinese)
[1] JIANG Peng, ZHANG Peng, YAO JinBao, WU Lei, HE Yi, LI Chang, MA HongXiang, ZHANG Xu. Phenotypic Characteristics and Related Gene Analysis of Ningmai Series Wheat Varieties [J]. Scientia Agricultura Sinica, 2022, 55(2): 233-247.
[2] XiaoChuan LI,ChaoHai WANG,Ping ZHOU,Wei MA,Rui WU,ZhiHao SONG,Yan MEI. Deciphering of the Genetic Diversity After Field Late Blight Resistance Evaluation of Potato Breeds [J]. Scientia Agricultura Sinica, 2022, 55(18): 3484-3500.
[3] YingLing WAN,MengTing ZHU,AiQing LIU,YiJia JIN,Yan LIU. Phenotypic Diversity Analysis of Chinese Ornamental Herbaceous Peonies and Its Germplasm Resource Evaluation [J]. Scientia Agricultura Sinica, 2022, 55(18): 3629-3639.
[4] HU GuangMing,ZHANG Qiong,HAN Fei,LI DaWei,LI ZuoZhou,WANG Zhi,ZHAO TingTing,TIAN Hua,LIU XiaoLi,ZHONG CaiHong. Screening and Application of Universal SSR Molecular Marker Primers in Actinidia [J]. Scientia Agricultura Sinica, 2022, 55(17): 3411-3425.
[5] CHEN Xu,HAO YaQiong,NIE XingHua,YANG HaiYing,LIU Song,WANG XueFeng,CAO QingQin,QIN Ling,XING Yu. Association Analysis of Main Characteristics of Bur and Nut with SSR Markers in Chinese Chestnut [J]. Scientia Agricultura Sinica, 2022, 55(13): 2613-2628.
[6] XU Xiao,REN GenZeng,ZHAO XinRui,CHANG JinHua,CUI JiangHui. Accurate Identification and Comprehensive Evaluation of Panicle Phenotypic Traits of Landraces and Cultivars of Sorghum bicolor (L.) Moench in China [J]. Scientia Agricultura Sinica, 2022, 55(11): 2092-2108.
[7] TANG XiuJun,FAN YanFeng,JIA XiaoXu,GE QingLian,LU JunXian,TANG MengJun,HAN Wei,GAO YuShi. Genetic Diversity and Origin Characteristics of Chicken Species Based on Mitochondrial DNA D-loop Region [J]. Scientia Agricultura Sinica, 2021, 54(24): 5302-5315.
[8] LI XinYuan, LOU JinXiu, LIU QingYuan, HU Jian, ZHANG YingJun. Genetic Diversity Analysis of Rhizobia Associated with Medicago sativa Cultivated in Northeast and North China [J]. Scientia Agricultura Sinica, 2021, 54(16): 3393-3405.
[9] WANG FuQiang,ZHANG Jian,WEN ChangLong,FAN XiuCai,ZHANG Ying,SUN Lei,LIU ChongHuai,JIANG JianFu. Identification of Grape Cultivars Based on KASP Markers [J]. Scientia Agricultura Sinica, 2021, 54(13): 2830-2842.
[10] YANG Tao,HUANG YaJie,LI ShengMei,REN Dan,CUI JinXin,PANG Bo,YU Shuang,GAO WenWei. Genetic Diversity and Comprehensive Evaluation of Phenotypic Traits in Sea-Island Cotton Germplasm Resources [J]. Scientia Agricultura Sinica, 2021, 54(12): 2499-2509.
[11] CUI YiPing,PENG AiTian,SONG XiaoBing,CHENG BaoPing,LING JinFeng,CHEN Xia. Investigation on Occurrence of Citrus Huanglongbing and Virus Diseases, and Prophage Genetic Diversity of Huanglongbing Pathogen in Meizhou, Guangdong [J]. Scientia Agricultura Sinica, 2020, 53(8): 1572-1582.
[12] JiaYing CHANG,ShuSen LIU,Jie SHI,Ning GUO,HaiJian ZHANG,HongXia MA,ChunFeng YANG. Pathogenicity and Genetic Diversity of Bipolaria maydis in Sanya, Hainan and Huang-Huai-Hai Region [J]. Scientia Agricultura Sinica, 2020, 53(6): 1154-1165.
[13] MoRan XU,RuiMing LIN,FengTao WANG,Jing FENG,ShiChang XU. Evaluation of Resistance to Stripe Rust and Genetic Diversity and Detection of Resistance Genes in 103 Wheat Cultivars (Lines) [J]. Scientia Agricultura Sinica, 2020, 53(4): 748-760.
[14] GAO Yuan,WANG DaJiang,WANG Kun,CONG PeiHua,ZHANG CaiXia,LI LianWen,PIAO JiCheng. Genetic Diversity and Phylogenetics of Malus baccata (L.) Borkh Revealed by Chloroplast DNA Variation [J]. Scientia Agricultura Sinica, 2020, 53(3): 600-611.
[15] WANG BaoBao,GUO Cheng,SUN SuLi,XIA YuSheng,ZHU ZhenDong,DUAN CanXing. The Genetic Diversity, Pathogenicity, and Toxigenic Chemotypes of Fusarium graminearum Species Complex Causing Maize Ear Rot [J]. Scientia Agricultura Sinica, 2020, 53(23): 4777-4790.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd