Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (11): 2394-2402.doi: 10.3864/j.issn.0578-1752.2013.11.025

• RESEARCH NOTES • Previous Articles    

Genetic Diversity of Large-Flowered Chrysanthemum Cultivars Revealed by ISSR Analysis

 LUO  Xin-Yan, WANG  Chen, DAI  Si-Lan, LI  Bao-Qin, LIU  Qian-Qian, ZHU  Jun, LU  Jie   

  1. College of Landscape, Beijing Forestry University/National Flower Engineering Research Center, Beijing 100083
  • Received:2012-06-27 Online:2013-06-01 Published:2013-04-11

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Abstract: 【Objective】 Large-flowered chrysanthemum processes numerous cultivars with complex morphological types. This study aims to facilitate the research on classification and evolution history of chrysanthemum cultivars by a probe in genetic diversity of chrysanthemum cultivars belonging to different floral types respectively.【Method】 The genetic diversity of 150 large-flowered chrysanthemum cultivars belonging to 5 floral types were investigated using 10 primers with clear bands and steady repetitiveness chosen from 50 ISSR primers. 【Result】 A total of 229 bands were amplified, of which 220 were polymorphic loci. The main index were: PPB＝96.07%, He＝0.3485, I＝0.5154(at species level). The genetic variation in each floral types is quite high, in which the Flat-petal group is the highest (PPB＝95.20%), whereas the Anemone-petal group (PPB＝86.90%) and the Spoon-petal group (PPB＝89.52%) were the lowest. The level of genetic differentiation among floral types was very low, and genetic diversity mainly derived from variations of cultivars at flower type level. The result of UPGMA cluster was highly relevant to morphological variations among floral types. 【Conclusion】 The result of the research shows that genetic diversity of large-flowered chrysanthemum cultivars can be revealed well and truly by ISSR molecular marker，which processes of great meaning to research of classification, identification and evolution of chrysanthemum cultivars.

Key words: large-flowered chrysanthemum , floral type , genetic diversity , ISSR

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