Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (8): 1572-1582.doi: 10.3864/j.issn.0578-1752.2020.08.007

• PLANT PROTECTION • Previous Articles     Next Articles

Investigation on Occurrence of Citrus Huanglongbing and Virus Diseases, and Prophage Genetic Diversity of Huanglongbing Pathogen in Meizhou, Guangdong

CUI YiPing,PENG AiTian(),SONG XiaoBing,CHENG BaoPing,LING JinFeng,CHEN Xia   

  1. Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640
  • Received:2019-09-16 Accepted:2019-11-18 Online:2020-04-16 Published:2020-04-29
  • Contact: AiTian PENG


【Objective】The objective of this study is to investigate the impacts of citrus Huanglongbing (HLB) and virus diseases on citrus production in Meizhou City of Guangdong Province, and to reveal the local ‘Candidatus Liberibacter asiaticus’ (CLas, the pathogen of HLB in China) strains genetic diversity based on the prophage region.【Method】The primers were designed with 16S rDNA as the template, and the incidence of HLB in different sampling sites in Meizhou City in the 3 years from 2017 to 2019 was detected by qPCR. After RNA extraction, RT-PCR followed by ordinary PCR were applied to detect Citrus tristeza virus (CTV), Citrus exocortis viroid (CEVd), Citrus chlorotic dwarf-associated virus (CCDaV), Citrus tatter leaf virus (CTLV) and Citrus yellow vein clearing virus (CYVCV) using their own reported primers, separately. Two pairs of primers (Lap-TJ-F/Lap-TJ-R1 and Lap-TJ-F/Lap-TJ-R2) were designed based on the supervariant gene regions corresponding to two prophage types (SC1 and SC2), and the genetic diversity of CLas prophage regions was analyzed using ordinary PCR method.【Result】The results of CLas detection showed that only the sample from one orchard (Honey pummelo base in Shunxing Company in Dapu County) in September, 2018 was free, other inspected areas in Meizhou all had CLas. Overall, the CLas detection rate of navel orange in Meizhou was 55.3%, the honey pummelo was 61.5%, and the Shatian pummelo was 61.7%. Among them, the CLas detection rate of honey pummelo and Shatian pummelo collected from Datuo Town, Pingyuan County in 2017 was 100%, the detection rate of honey pummelo and Shatian pummelo in Baima Town, Xingning City was 100% and 80%, respectively. The detection rate of samples in other sampling areas was 16.7%-83.3%. Although the detection rate in some areas of Meizhou was high, the content of pathogenic bacteria was not high, most of them were at the early and middle stages of HLB, which belonged to a preventable and controllable stage. In the same region, the total detection rate of citrus viruses in Meizhou City was not high. CTV, CTLV and CYVCV were the main viruses in Meizhou, CEVd and CCDaV were not detected. The detection rate of CTV, CTLV and CYVCV on navel orange was 78.9%, 7.9% and 21.1%, on honey pummelo was 15.4%, 25.0%, and 9.6%, on Shatian pummelo was 6.4%, 2.1%, and 4.3%, respectively. Through prophage genetic diversity analysis of CLas strains in Meizhou City, four CLas prophage amplicon types (SC1-1, SC1-2, SC2-1 and SC2-2) were observed in the CLas from all the tested samples, SC2-1 represented the predominant CLas type in navel orange and Shatian pummelo in Meizhou City, and SC1-1 was predominant in honey pummelo.【Conclusion】The citrus HLB in Meizhou citrus producing area is currently at a controllable stage. The prophage of its pathogenic CLas strain is unique in different varieties. Due to the existence of citrus virus diseases during spot inspection, seedling supervision needs to be strengthened during the planting process and effective measures should be taken to prevent and control HLB and Diaphorina citri.

Key words: citrus Huanglongbing, Candidatus Liberibacter asiaticus’ (CLas), citrus virus disease, prophage, genetic diversity

Table 1

The detailed message of part of sample locations"

Crop years (a)
Area (hm2)
Pingyuan County
仁居镇飞龙村Feilong Village, Renju Town 脐橙Navel orange >2 100
仁居镇六吉村Liuji Village, Renju Town >1 100
Meixian District
石扇镇西南村Xi’nan Village, Shishan Town 蜜柚Honey pummelo >20 100
人民广场森林公园People’s Square Forest Park >5 >10
石扇镇西南村Xi’nan Village, Shishan Town 沙田柚Shatian pummelo >2 300
城东镇玉水村Yushui Village, Chengdong Town >25 >10
大埔县Dapu County 大东镇东光村Dongguang Village, Dadong Town >9 1000

Table 2

Detection of CLas in main citrus producing areas in Meizhou City"

采样时间Sampling time 采集地
样品数(个)Number 检出率
Detection rate
HLB content
(×103 copies/g)
Ct range
2019-06 平远县仁居镇飞龙村Feilong/Renju/Pingyuan 脐橙
Navel orange
11 63.6 0.069-735 21.18-35.27
10 30.0 0.078-0.123 34.39-35.08
Honey pummelo
10 70.0 0.14-393 22.13-34.19
People’s Square Forest Park in Meixian District
12 66.7 0.073-277 22.66-35.17
Shatian pummelo
10 60.0 0.073-576 21.55-35.18
10 80.0 0.072-856 20.95-35.20
15 60.0 0.069-1.02 31.18-35.27
2018-01 平远县仁居镇李氏家庭农场
Family Farm of LI/Renju Town/Pingyuan County
脐橙× Navel orange 11 54.5 0.072-49.7 25.27-35.19
脐橙* Navel orange 6 83.3 0.071-0.76 31.62-35.22
Nanfu/ Yanyang/Meixian
Honey pummelo
6 16.7 0.059 35.50
5 80.0 0.042-0.31 32.98-36.02
5 60.0 0.135-1.7 30.43-34.24
2018-09 大埔顺兴公司蜜柚基地
Honey pummelo base in Shunxing Company in Dapu County
5 0 0 0
沙田柚Shatian pummelo 5 0 0 0
2017-07 兴宁市白马镇
蜜柚Honey pummelo 6 100.0 0.199-1036 20.66-32.26
沙田柚Shatianpummelo 5 80.0 0.052-0.683 31.78-35.68
2017-08 平远县大拓镇
蜜柚Honey pummelo 3 100.0 0.232-1106 20.56-33.42
沙田柚Shatian pummelo 2 100.0 0.18-1742 19.87-33.81
2017-2019 梅州市
Meizhou City
脐橙Navel orange 38 55.3 0.069-735 21.18-35.27
蜜柚Honey pummelo 52 61.5 0.042-1106 20.56-36.02
沙田柚Shatian pummelo 47 61.7 0.052-1742 19.87-35.68

Table 3

Detection of citrus viruses in main citrus producing areas in Meizhou City"

Location (Village/Town/County)
检出率Detection rate (%)
Navel orange
11 100.0 0 0 0 0
10 50.0 0 0 0 0
Honey pummelo
10 50.0 0 0 0 0
People’s Square Forest Park in Meixian County
10 0 0 0 0 0
Shatian pummelo
12 0 0 0 0 0
10 0 0 0 0 0
15 0 0 0 0 0
Family Farm of LI, Renju Town, Pingyuan County
脐橙× Navel orange 11 81.8 0 0 9.1 72.7
脐橙* Navel orange 6 83.3 0 0 33.3 0
Honey pummelo
6 0 0 0 50 0
5 0 0 0 100 0
5 0 0 0 60 0
Honey pummelo base in Shunxing Company in Dapu County
5 0 0 0 20 40
沙田柚Shatian pummelo 5 0 0 0 0 0
蜜柚Honey pummelo 3 100.0 0 0 0 100.0
沙田柚Shatian pummelo 2 100.0 0 0 50.0 100.0
蜜柚Honey pummelo 6 0 0 0 20.0 0
沙田柚Shatian pummelo 5 10.0 0 0 0 0
Meizhou City
脐橙Navel orange 38 78.9 0 0 7.9 21.1
蜜柚Honey pummelo 52 15.4 0 0 25.0 9.6
沙田柚Shatian pummelo 47 6.4 0 0 2.1 4.3

Table 4

Sample information and amplicon types of partial CLas strains from Meizhou City"

Ct-value CLas 扩增类型
Amplified type
qPCR Ordinary PCR
Zhongluotan Town, Baiyun District, Guangzhou City
砂糖橘Sugar orange Z1 22.08 A+ A+ SC2-1 (2)
默克特Murcott Z2 25.89 A+ A+ SC2-1(2)
蜜柚Honey pummelo Z3 22.08 A+ A+ SC2-1
Renju Town, Pingyuan County
脐橙Navel orange R1 21.18 A+ A+ SC2-1
R2 35.20 A+ A- SC2-1
R3 34.80 A+ A- SC2-1
R4 N A- A- -
Shishan Town, Meixian District
蜜柚Honey pummelo SM1 22.13 A+ A+ SC1-1
SM2 32.69 A+ A- SC1-1
沙田柚Shatian pummelo S1 21.55 A+ A+ SC2-1
S2 33.96 A+ A- SC2-1
Chengdong Town, Meixian District
沙田柚Shatian pummelo C1 31.68 A+ A- SC2-1
C2 34.06 A+ A- SC1-2
People’s Square Forest Park in Meixian District
蜜柚Honey pummelo G1 22.66 A+ A+ SC2-1
G2 34.60 A+ A- SC2-1
Dadong Town, Dapu County
蜜柚Honey pummelo D1 31.18 A+ A- SC2-1
D2 34.92 A+ A- -

Fig. 1

Electrophoresis profile of representative Meizhou citrus CLas strains from PCR amplification with three primer sets targeting three gene regions"

Fig. 2

Phylogenetic tree of prophage gene sequences constructed by neighbor-joining method"

[1] BLAUSTEIN R A, LORCA G L, TEPLITSKI M . Challenges for managing Candidatus Liberibacter spp. (Huanglongbing disease pathogen): Current control measures and future directions. Phytopathology, 2018,108(4):424-435.
[2] DA GRACA J V, DOUHAN G W, HALBERT S E, KEREMANE M L, LEE R F, VIDALAKIS G, ZHAO H W . Huanglongbing: An overview of a complex pathosystem ravaging the world’s citrus. Journal of Integrative Plant Biology, 2016,58(4):373-387.
[3] JAGOUEIX S, BOVE J M, GARNIER M . The phloem-limited bacterium of greening disease of citrus is a member of the α subdivision of the Proteobacteria. International Journal of Systematic Bacteriology, 1994,44(3):379-386.
[4] DO CARMO TEIXEIRA D, SAILLARD C, EVEILLARD S, DANET J L, DA COSTA P I, AYRES A J, BOVÉ J . ‘ Candidatus Liberibacter americanus’, associated with citrus Huanglongbing (greening disease) in São Paulo State, Brazil. International Journal of Systematic and Evolutionary Microbiology, 2005,55(5):1857-1862.
[5] DUAN Y P, ZHOU L J, HALL D G, LI W, DODDAPANENI H, LIN H, LIU L, VAHLING C M, GABRIEL D W, WILLIAMS K P, DICKERMAN A, SUN Y, GOTTWALD T . Complete genome sequence of citrus Huanglongbing bacterium ‘ Candidatus Liberibacter asiaticus’obtained through metagenomics. Molecular Plant-Microbe Interactions, 2009,22(8):1011-1020.
[6] 陈燕玲, 郑正, 许美容, 邓晓玲 . 杂柑‘W·默科特’黄龙病菌分子鉴定和遗传多样性分析. 园艺学报, 2019,46(6):1155-1162.
CHEN Y L, ZHENG Z, XU M R, DENG X L . Molecular identification and genetic diversity of ‘ Candidatus Liberibacter asiaticus’ in W. Murcott tangerine. Acta Horticulturae Sinica, 2019,46(6):1155-1162. (in Chinese)
[7] 丁芳, 洪霓, 钟云, 易干军, 王国平 . 中国柑橘黄龙病病原16S rDNA序列研究. 园艺学报, 2008,35(5):649-654.
DING F, HONG N, ZHONG Y, YI G J, WANG G P . Studies on 16S rDNA sequence of citrus Huanglongbing bacteria in China. Acta Horticulturae Sinica, 2008,35(5):649-654. (in Chinese)
[8] TOMIMURA K, MIYATA S, FURUYA N, KUBOTA K, OKUDA M, SUBANDIYAH S, HUNG T H, SU H J, IWANAMI T . Evaluation of genetic diversity among ‘ Candidatus Liberibacter asiaticus’ isolates collected in Southeast Asia. Phytopathology, 2009,99(9):1062-1069.
[9] 姚锦爱, 谢荔岩, 郑璐平, 胡奇勇 . 柑桔黄龙病亚洲种病原(Candidatus Liberobacter asiaticus) 16S rDNA的PCR-SSCP分析. 福建农业学报, 2008,23(3):331-333.
YAO J A, XIE L Y, ZHENG L P, HU Q Y . PCR-SSCP analysis on 16S rDNA of citrus Huanglongbing pathogen ( Candidatus Liberibacter asiaticum). Fujian Journal of Agricultural Sciences, 2008,23(3):331-333. (in Chinese)
[10] VILLECHANOUX S, GARNIER M, LAIGRET F, RENAUDIN J, BOVÉ J M . The genome of the non-cultured, bacterial-like organism associated with citrus greening disease contains the nusG-rplKAJL- rpoBC gene cluster and the gene for a bacteriophage type DNA polymerase. Current Microbiology, 1993,26(3):161-166.
[11] OKUDA M, MATSUMOTO M, TANAKA Y, SUBANDIYAH S, IWANAMI T . Characterization of the tufB-secE-nusG-rplKAJL-rpoB gene cluster of the citrus greening organism and detection by loop-mediated isothermal amplification. Plant Disease, 2005,89(7):705-711.
[12] TEIXEIRA D C, EVEILLARD S, SIRAND-PUGNET P, WULFF A, SAILLARD C, AYRES A J, BOVÉ J M . The tufB-secE-nusG- rplKAJL-rpoB gene cluster of the liberibacters: Sequence comparisons, phylogeny and speciation. International Journal of Systematic and Evolutionary Microbiology, 2008,58(6):1414-1421.
[13] PLOTTO A, BALDWIN E, MCCOLLUM G, MANTHEY J, NARCISO J, IREY M . Effect of Liberibacter infection (Huanglongbing or “greening” disease) of citrus on orange juice flavor quality by sensory evaluation. Journal of Food Science, 2010,75(4):S220-S230.
[14] 黄永辉, 程保平, 彭埃天, 宋晓兵, 凌金锋, 陈霞 . 广东不同地区柑橘黄龙病菌的遗传多样性分析. 植物保护, 2014,40(1):60-64.
HUANG Y H, CHENG B P, PENG A T, SONG X B, LING J F, CHEN X . Genetic diversity of Candidatus liberibacter isolates from different geographical regions of Guangdong Province. Plant Protection, 2014,40(1):60-64. (in Chinese)
[15] 董俊, 戴良英, 易图永 . 湖南省柑橘黄龙病菌M94319和16S rDNA序列的扩增和分析. 湖南农业科学, 2015(8):1-3, 7.
DONG J, DAI L Y, YI T Y . Amplification and analysis ofM94319 and 16S rDNA sequence of citrus Huanglongbing in Hunan. Hunan Agricultural Sciences, 2015(8):1-3, 7. (in Chinese)
[16] 谭锦, 王雪峰, 苏华楠, 李中安, 周常勇 . 中国柑橘黄龙病病原菌两个原噬菌体超变异基因遗传多样性. 中国农业科学, 2013,46(18):3784-3792.
TAN J, WANG X F, SU H N, LI Z G, ZHOU C Y . Genetic diversity of two hypervariable genes from prophage regions of ‘ Candidatus Liberibacter asiaticus’ in China. Scientia Agricultura Sinica, 2013,46(18):3784-3792. (in Chinese)
[17] XU M R, ZHENG Z, LI X Y, HONG H X, DENG X L . Intraspecific genetic diversity analysis of ‘ Candidatus Liberibacter asiaticus’ by short tandem repeats and PAGE. Acta Pthytopathologica Sinica, 2014,44(6):609-619.
[18] 黄爱军, 苏华楠, 王雪峰, 唐科志, 李中安, 周常勇 . 基于SSR标记的中国亚洲韧皮杆菌种群结构研究. 中国农业科学, 2014,47(22):4488-4494.
HUANG A J, SU H N, WANG X F, TANG K Z, LI Z G, ZHOU C Y . Population structure of ‘ Candidatus Liberibacter asiaticus’ in China revealed by SSR markers. Scientia Agricultura Sinica, 2014,47(22):4488-4494. (in Chinese)
[19] 卢占军, 刘映雪, 丁鹏, 赵培娅, 黄爱军, 苏华楠, 钟八莲 . 基于SSR标记的赣南柑桔黄龙病菌遗传多样性分析. 中国南方果树, 2016,45(5):1-6.
LU Z J, LIU Y X, DING P, ZHAO P Y, HUANG A J, SU H N, ZHONG B L . Genetic diversity of Candidatus Liberibacter asiaticus in Gannan using SSR markers. South China Fruits, 2016,45(5):1-6. (in Chinese)
[20] 李国华, 谢岳昌, 孙中兴, 徐秋明 . 梅州市柑桔产业现状及发展对策. 广东农业科学, 2010(9):244-246.
LI G H, XIE Y C, SUN Z X, XU Q M . The present situation of citrus industry in Meizhou and its development countermeasures. Guangdong Agricultural Sciences, 2010(9):244-246. (in Chinese)
[21] 沈兆敏 . 2012年我国柑橘生产现状浅析及持续发展对策. 果农之友, 2013(10):38-39, 41.
SHEN Z M . Analysis on the present situation of Chinese citrus production in 2012 and its sustainable development countermeasures. Fruit Growers’ Friend, 2013(10):38-39, 41. (in Chinese)
[22] 李月, 张志标, 钟进良, 黄静, 杜小珍, 张雄基, 刘蕊, 温清英, 马瑞丰 . 梅州柚产业现状及发展对策. 现代农业科技, 2019(3):75-76, 78.
LI Y, ZHANG Z B, ZHONG J L, HUANG J, DU X Z, ZHANG X J, LIU R, WEN Q Y, MA R F . Current status of Meizhou pummelo industry and its development countermeasures. Modern Agricultural Science and Technology, 2019(3):75-76, 78. (in Chinese)
[23] LI W, HARTUNG J S, LEVY L . Quantitative real-time PCR for detection and identification of Candidatus Liberibacter species associated with citrus Huanglongbing. Journal of Microbiological Methods, 2006,66(1):104-115.
[24] HOCQUELLET A, TOORAWA P, BOVÉ J M, GARNIER M . Detection and identification of the two Candidatus Liberobacter species associated with citrus huanglongbing by PCR amplification of ribosomal protein genes of the β operon. Molecular and Cellular Probes, 1999,13:373-379.
[25] TAMURA K, DUDLEY J, NEI M, KUMAR S . MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 2007,24(8):1596-1599.
[26] 肖远辉, 曾继吾, 张秋明, 周碧容 . 柑橘衰退病、裂皮病和碎叶病的多重RT-PCR检测方法研究. 植物病理学报, 2007,37(1):31-35.
XIAO Y H, ZENG J W, ZHANG Q M, ZHOU B R . Simultaneous detection of Citrus tristeza virus (CTV), Citrus exocortis viroid (CEVd) and Citrus tatter leaf virus (CTLV) by multiplex RT-PCR. Acta Phytopathologica Sinica, 2007,37(1):31-35. (in Chinese)
[27] LOCONSOLE G, SALDARELLI P, DODDAPANENI H, SAVINO V, MARTELLI G P, SAPONARI M . Identification of a single-stranded DNA virus associated with citrus chlorotic dwarf disease, a new member in the family Geminiviridae. Virology, 2012,432(1):162-172.
[28] 陈洪明, 王雪峰, 周彦, 周常勇, 郭俊, 李中安 . 尤力克柠檬上一种新病害的生物学特性及RT-PCR检测. 植物保护学报, 2015,42(4):557-563.
CHEN H M, WANG X F, ZHOU Y, ZHOU C Y, GUO J, LI Z G . Biological characterization and RT-PCR detection of a new disease of Eureka lemon. Journal of Plant Protection, 2015,42(4):557-563. (in Chinese)
[29] 崔一平, 彭埃天, 李子力, 凌金峰, 宋晓兵, 郭泽成, 陈霞, 程保平, 殷瑜 . 海南省柑橘主产区黄龙病和病毒病的发生危害情况调研初报. 植物保护, 2019,45(4):236-242.
CUI Y P, PENG A T, LI Z L, LING J F, SONG X B, GUO Z C, CHEN X, CHENG B P, YIN Y . Survey on HLB and virus disease of main citrus production area in Hainan Province. Plant Protection, 2019,45(4):236-242. (in Chinese)
[30] 桑文, 刘燕梅, 邱宝利 . 柑橘木虱绿色防控技术研究进展. 应用昆虫学报, 2018,55(4):557-564.
SANG W, LIU Y M, QIU B L . Advance in the eco-friendly management of Diaphorina citri. Chinese Journal of Applied Entomology, 2018,55(4):557-564. (in Chinese)
[1] LIU Jiao,LIU Chang,CHEN Jin,WANG MianZhi,XIONG WenGuang,ZENG ZhenLing. Distribution Characteristics of Prophage in Multidrug Resistant Escherichia coli as well as Its Induction and Isolation [J]. Scientia Agricultura Sinica, 2022, 55(7): 1469-1478.
[2] HUANG JiaQuan,LI Li,WU FengNian,ZHENG Zheng,DENG XiaoLing. Proliferation of Two Types Prophage of ‘Candidatus Liberibacter asiaticus’ in Diaphorina citri and their Pathogenicity [J]. Scientia Agricultura Sinica, 2022, 55(4): 719-728.
[3] JIANG Peng, ZHANG Peng, YAO JinBao, WU Lei, HE Yi, LI Chang, MA HongXiang, ZHANG Xu. Phenotypic Characteristics and Related Gene Analysis of Ningmai Series Wheat Varieties [J]. Scientia Agricultura Sinica, 2022, 55(2): 233-247.
[4] XiaoChuan LI,ChaoHai WANG,Ping ZHOU,Wei MA,Rui WU,ZhiHao SONG,Yan MEI. Deciphering of the Genetic Diversity After Field Late Blight Resistance Evaluation of Potato Breeds [J]. Scientia Agricultura Sinica, 2022, 55(18): 3484-3500.
[5] YingLing WAN,MengTing ZHU,AiQing LIU,YiJia JIN,Yan LIU. Phenotypic Diversity Analysis of Chinese Ornamental Herbaceous Peonies and Its Germplasm Resource Evaluation [J]. Scientia Agricultura Sinica, 2022, 55(18): 3629-3639.
[6] HU GuangMing,ZHANG Qiong,HAN Fei,LI DaWei,LI ZuoZhou,WANG Zhi,ZHAO TingTing,TIAN Hua,LIU XiaoLi,ZHONG CaiHong. Screening and Application of Universal SSR Molecular Marker Primers in Actinidia [J]. Scientia Agricultura Sinica, 2022, 55(17): 3411-3425.
[7] CHEN Xu,HAO YaQiong,NIE XingHua,YANG HaiYing,LIU Song,WANG XueFeng,CAO QingQin,QIN Ling,XING Yu. Association Analysis of Main Characteristics of Bur and Nut with SSR Markers in Chinese Chestnut [J]. Scientia Agricultura Sinica, 2022, 55(13): 2613-2628.
[8] XU Xiao,REN GenZeng,ZHAO XinRui,CHANG JinHua,CUI JiangHui. Accurate Identification and Comprehensive Evaluation of Panicle Phenotypic Traits of Landraces and Cultivars of Sorghum bicolor (L.) Moench in China [J]. Scientia Agricultura Sinica, 2022, 55(11): 2092-2108.
[9] LI ZhenXi,LI WenTing,HUANG JiaQuan,ZHENG Zheng,XU MeiRong,DENG XiaoLing. Detection of ‘Candidatus Liberibacter asiaticus’ by Membrane Adsorption Method Combined with Visual Loop-Mediated Isothermal Amplification [J]. Scientia Agricultura Sinica, 2022, 55(1): 74-84.
[10] ZHAO Ke,ZHENG Lin,DU MeiXia,LONG JunHong,HE YongRui,CHEN ShanChun,ZOU XiuPing. Response Characteristics of Plant SAR and Its Signaling Gene CsSABP2 to Huanglongbing Infection in Citrus [J]. Scientia Agricultura Sinica, 2021, 54(8): 1638-1652.
[11] TANG XiuJun,FAN YanFeng,JIA XiaoXu,GE QingLian,LU JunXian,TANG MengJun,HAN Wei,GAO YuShi. Genetic Diversity and Origin Characteristics of Chicken Species Based on Mitochondrial DNA D-loop Region [J]. Scientia Agricultura Sinica, 2021, 54(24): 5302-5315.
[12] LI XinYuan, LOU JinXiu, LIU QingYuan, HU Jian, ZHANG YingJun. Genetic Diversity Analysis of Rhizobia Associated with Medicago sativa Cultivated in Northeast and North China [J]. Scientia Agricultura Sinica, 2021, 54(16): 3393-3405.
[13] WANG FuQiang,ZHANG Jian,WEN ChangLong,FAN XiuCai,ZHANG Ying,SUN Lei,LIU ChongHuai,JIANG JianFu. Identification of Grape Cultivars Based on KASP Markers [J]. Scientia Agricultura Sinica, 2021, 54(13): 2830-2842.
[14] YANG Tao,HUANG YaJie,LI ShengMei,REN Dan,CUI JinXin,PANG Bo,YU Shuang,GAO WenWei. Genetic Diversity and Comprehensive Evaluation of Phenotypic Traits in Sea-Island Cotton Germplasm Resources [J]. Scientia Agricultura Sinica, 2021, 54(12): 2499-2509.
[15] CaiLing TENG,Xi ZHONG,HaoDi WU,Yan HU,ChangYong ZHOU,XueFeng WANG. Biologic and Transcriptomic Analysis of Citrus hystrix Responses to ‘Candidatus Liberibacter asiaticus’ at Different Infection Stages [J]. Scientia Agricultura Sinica, 2020, 53(7): 1368-1380.
Full text



No Suggested Reading articles found!