Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (22): 4574-4586.doi: 10.3864/j.issn.0578-1752.2012.22.004

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Effect of Tillage in Fallow Period on Soil Water, Post-Anthesis Proline Accumulation and Grains Protein Accumulation in Dryland Wheat

 ZHAO  Hong-Mei, GAO  Zhi-Qiang, SUN   Min, ZHAO  Wei-Feng, LI   Qing, DENG   Yan, YANG  Zhen-Ping   

  1. College of Crop Science, Shanxi Agricultural University, Taigu 030801, Shanxi
  • Received:2012-06-26 Online:2012-11-15 Published:2012-09-10

PDF

843

Cited

7

Abstract: 【Objective】 A field plot experiment was conducted to study the effect of tillage in fallow period at different times on soil water storage, post-anthesis proline accumulation in flag leaves and grains and grains protein accumulation in winter wheat. The study will supply a new way for soil moisture conservation of one time in fallow period and a theoretical basis for improving yield and quality of dryland wheat.【Method】The effects of two treatment stages including 15 days and 45 days after wheat harvest, two tillage methods including deep tillage and subsoiling (no tillage as control) on soil water storage, post-anthesis proline accumulation in flag leaves and grains, protein accumulation, the change of activity of glutamine synthetase (GS) and activity of glutamate dehydrogenase(GDH), grains protein and the relation between proline content and nitrogen metabolism enzyme were determined. 【Result】The results showed that soil water storage at the depth of 0-300 cm was improved by tillage in fallow period, especially in dry year and deep tillage in 45 days after wheat harvest had better soil moisture conservation effects. Proline content in flag leaves, proline content in grains of post-anthesis 5-15 d and activity of GDH in flag leaves and grains were reduced, however, GS in grains and protein yield in grains were enhanced by tillage in fallow period. Tillage stage had obvious difference in activity of GS and grain protein content in different rainfall years. Activity of GS in flag leaves and protein content in grains were reduced by tillage in dry year. In abundant water year the activity of GS in flag leaves and protein content in grains were reduced in 15 days after wheat harvest and enhanced in 45 days after wheat harvest by tillage. Under tillage in fallow period, the correlation between soil water storage before sowing and proline content in grains was higher than that of proline content in flag leaves by tillage in fallow period. The relation between proline content in flag leaves and the activity of GDH in flag leaves, proline content in grains and the activity of GS in flag leaves was closed in 15 days after wheat harvest by tillage. The relation between proline content in flag leaves and the activity of GDH in grains was closed in 45 days after wheat harvest by tillage. But there were no relation between proline in grains and two enzymes in 45 days after wheat harvest by tillage. 【Conclusion】The above results suggested that tillage in fallow period especially in dry year had better soil moisture conservation effects, benefit for decreasing the proline content in flag leaves and proline content in grains at early filling stage, relieving drought degree of wheat suffered. As proline content had a great relation with nitrogen metabolism enzyme, so grains protein accumulation was affected. Tillage stage had great regulation effects on soil water, post-anthesis proline accumulation and grains protein accumulation. Deep tillage after rainfall benefit for grains protein formation.

Key words: dryland wheat , fallow period , tillage , proline content , nitrogen metabolism , protein content

[1]毛红玲, 李 军, 贾志宽, 王 蕾. 旱作麦田保护性耕作蓄水保墒和增产增收效应. 农业工程学报, 2010, 26(8): 44-51.

Mao H L, Li J, Jia Z K, Wang L. Soil water conservation effect, yield and income increments of conservation tillage measures on dryland wheat field. Transactions Chinese Society Agricultural Engineering, 2010, 26(8): 44-51. (in Chinese)

[2]Su Z Y, Zhang J S, Wu W L, Cai D X, Lv J J, Jiang G H, Huang J, Gao J, Hartmann R, Gabriels D. Effects of conservation tillage practices on winter wheat water-use ef?ciency and crop yield on the Loess Plateau, China. Agricultural Water Management, 2007, 87: 307-314.

[3]侯贤清, 王 维, 韩清芳, 贾志宽, 严 波, 李永平, 苏 秦. 夏闲期轮耕对小麦田土壤水分及产量的影响. 应用生态学报, 2011, 22(10): 2524-2532.

Hou X Q, Wang W, Han Q F, Jia Z K, Yan B, Li Y P, Su Q. Effects of rotational tillage during summer fallow on wheat field soil water regime and grain yield. Chinese Journal of Applied Ecology, 2011, 22(10): 2524-2532. (in Chinese)

[4]侯贤清, 李 荣, 韩清芳, 王 维, 贾志宽. 夏闲期不同耕作模式对土壤蓄水保墒效果及作物水分利用效率的影响. 农业工程学报, 2012, 28(3): 94-100.

Hou X Q, Li R, Han Q F, Wang W, Jia Z K. Effects of different tillage patterns during summer fallow on soil water conservation and crop water use efficiency. Transactions Chinese Society Agricultural Engineering, 2012, 28(3): 94-100. (in Chinese)

[5]Hellmann H, Funck D, Rentsch D, Frommer W B. Hypersensitivity of an arabidopsis sugar signaling mutant toward exogenous proline application. Plant Physiology, 2000, 123: 779-790.

[6]Bates L S, Waldren R P, Teare I D. Rapid determination of free proline for water stress studies. Plant Soil, 1973, 39: 205-207.

[7]Lu B, Yuan Y, Zhang C, Ou J, Zhou W, Lin Q. Modulation of key enzymes involved in ammonium assimilation and carbon metabolism by low temperature in rice (Oryza sativa L.) roots. Plant Science, 2005, 169: 295-302.

[8]侯贤清, 韩清芳, 贾志宽, 李永平, 杨宝平. 半干旱区夏闲期不同耕作方式对土壤水分及小麦水分利用效率的影响. 干旱地区农业研究, 2009, 27(5): 52-58.

Hou X Q, Han Q F, Jia Z K, Li Y P, Yang B P. Effects of different tillage practices in summer fallow period on soil water and crop water use efficiency in semi-arid areas. Agricultural Research in the Arid Areas, 2009, 27(5): 52-58. (in Chinese)

[9]Jin K, Cornelis W M, Schiettecatte W, Lu J J, Yao Y Q, Wu H J, Gabriels D, De Neve S, Cai D X, Jin J Y, Hartmann R. Effects of different management practices on the soil–water balance and crop yield for improved dryland farming in the Chinese Loess Plateau. Soil and Tillage Research, 2007, 96: 131-144.

[10]王小彬, 蔡典雄, 金　轲, 吴会军, 白占国, 张灿军, 姚宇卿, 吕军杰, 王育红, 杨　波, Hartmann R, Gabriels D. 旱坡地麦田夏闲期耕作措施对土壤水分有效性的影响. 中国农业科学, 2003, 36(9): 1044-1049.

Wang X B, Cai D X, Jing K, Wu H J, Bai Z G, Zhang C J, Yao Y Q, Lu J J, Wang Y H, Yang B, Hartmann R, Gabriels D. Water availability for winter wheat affected by summer fallow tillage practices in sloping dryland. Scientia Agricultura Sinica, 2003, 36(9): 1044-1049. (in Chinese)

[11]许振柱, 于振文, 王 东, 张永丽. 灌溉条件对小麦籽粒蛋白质组分积累及品质的影响. 作物学报, 2003, 9(5): 682-687.

Xu Z Z, Yu Z W, Wang D, Zhang Y L. Effect of irrigation conditions on protein composition accumulation of grain and its quality in winter wheat. Acta Agronomica Sinica, 2003, 29(5): 682-687. (in Chinese)

[12]王晨阳, 郭天财, 彭 羽, 朱云集, 马冬云, 张灿军. 花后灌水对小麦籽粒品质性状及产量的影响. 作物学报, 2004, 30(10): 1031-1035.

Wang C Y, Guo T C, Peng Y, Zhu Y J, Ma D Y, Zhang C J. Effects of post-anthesis irrigation on grain quality indices and yield in winter wheat (Triticum aestivum L.). Acta Agronomica Sinica, 2004, 30(10): 1031-1035. (in Chinese)

[13]兰 涛, 姜 东, 谢祝捷, 戴廷波, 荆 奇, 曹卫星. 土壤干旱和渍水对不同专用小麦籽粒品质的影响. 水土保持学报, 2004, 18(1): 193-196.

Lan T, Jiang D, Xie Z J, Dai T B, Jing Q, Cao W X. Effects of post-anthesis drought and waterlogging on grain quality traits in different specialty wheat varieties. Journal Soil Water Conservation, 2004, 18(1): 193-196. (in Chinese)

[14]Xie Z J, Jiang D, Can W X, Dai T B, Jing Q. Effects of post anthesis soil water status on the activities of key regulatory enzymes of starch and protein accumulation in wheat grains. Journal Plant Physiologica Molecular Biology, 2003, 29: 309-316.

[15]张保军, 樊虎玲. 环境条件对小麦蛋白质的影响研究进展. 水土保持研究,2002,9(2): 61-63.

Zhang B J, Fan H L. Progress on the study of effects of environment conditions on wheat protein. Research Water and Soil Conservation, 2002, 9(2): 61-63. (in Chinese)

[16]赵 辉, 荆 奇, 戴廷波, 姜 东, 曹卫星. 花后高温和水分逆境对小麦籽粒蛋白质形成及其关键酶活性的影响. 作物学报, 2007, 33(11): 2021-2027.

Zhao H, Jing Q, Dai T D, Jiang D, Cao W X. Effects of post-anthesis high temperature and water stress on activities of key regulatory enzymes involved in protein formation in two wheat cultivars. Acta Agronomica Sinica, 2007, 33(11): 2021-2027. (in Chinese)

[17]付雪丽, 王晨阳, 郭天财, 朱云集, 马冬云, 王永华. 水氮互作对小麦籽粒蛋白质、淀粉含量及其组分的影响. 应用生态学报, 2008, 19(2): 317-322.

Fu X L, Wang C Y, Guo T C, Zhu Y J, Ma D Y, Wang Y H. Effects of water-nitrogen interaction on the contents and components of protein and starch in wheat grains. Chinese Journal Applied Ecology, 2008, 19(2): 317-322. (in Chinese)

[18]王小纯, 熊淑萍, 马新明. 不同氮素形态对专用小麦花后氮代谢关键酶活性及籽粒蛋白质含量的影响. 生态学报, 2005, 25(4): 802-808.

Wang X C, Xiong S P, Ma X M. Effects of different nitrogen forms on key enzyme activity involved in nitrogen metabolism and grain protein content in specialty wheat cultivars. Acta Ecologica Sinica, 2005, 25(4): 802-808. (in Chinese)

[19]马新明, 李 琳, 赵 鹏, 熊淑平, 郭 飞. 土壤水分对强筋小麦'豫麦34'氮素同化酶活性和籽粒品质的影响. 植物生态学报, 2005, 29(1): 48-53.

Ma X M, Li L, Zhao P, Xiong S P, Guo F. Effects of water control on activities of nitrogen assimilation enzymes and grain quality in winter wheat. Acta Phytoecologica Sinica, 2005,29(1): 48-53. (in Chinese)

[20]Verslues P E, Bray E A . Role of abscisic acid (ABA) and Arabidopsis thaliana ABA-insensitive loci in low water potential-induced ABA and proline accumulation. Journal of Experiment Botany, 2006, 57: 201-212.

[21]Wang Z Q, Yuan Y Z, Ou J Q, Lin Q H, Zhang C F. Glutamine synthetase and glutamate dehydrogenase contribute differentially to proline accumulation in leaves of wheat (Triticum aestivum) seedlings exposed to different salinity. Journal of Plant Physiology, 2007, 164: 695-701.

[22]Vendruscolo E C G, Schuster I, Pileggi M, Scapim C A, Molinari H B C, Marur C J, Vieira L G E. Stress-induced synthesis of proline confers tolerance to water de?cit in transgenic wheat. Journal of Plant Physiology, 2007, 164: 1367-1376.

[23]Delauney A J, Verma D P. Proline biosynthesis and osmoregulation in plants. Plant Journal, 1993, 4: 215-223.

[24]Kishor P B K, Sangam S, Amrutha R N, Laxmi1 P S, Naidu K R, Rao K R S S, Rao S, Reddy K J, Theriappan P, Sreenivasulu N. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plant growth and abiotic stress tolerance. Current Science, 2005, 88: 424-438.

[25]Larher F, Aziz A, Deleu C, Lemesle P, Ghaffar A, Bouchard F, Plasman M. Suppression of osmoinduced proline response of rape seed leaf discs by polyamines. Physiologia Plantarum, 1998, 102: 139-147.

[26]Damianos S S, Nikolaos V P, Konstantinos A P, Eleni D P, Loannis D D, Dimitris I Y, Antonios K, Anastasia K P, Euripides G S, Kalliopi A R A. Abiotic stress generates ROS that signal expression of anionic glutamate dehydrogenases to form glutamate for proline synthesis in tobacco and grapevine. Plant Cell, 2006, 18: 2767-2781.

[27]Brugière N, Dubois F, Limami A M, Lelandais M, Roux Y, Sangwan R S, Hirel B. Glutamine synthetase in the phloem plays a major role in controlling proline production. Plant Cell, 1999, 11: 1995-2011.
[1] HU Sheng,LI YangYang,TANG ZhangLin,LI JiaNa,QU CunMin,LIU LieZhao. Genome-Wide Association Analysis of the Changes in Oil Content and Protein Content Under Drought Stress in Brassica napus L. [J]. Scientia Agricultura Sinica, 2023, 56(1): 17-30.
[2] YU QiLong,HAN YingYan,HAO JingHong,QIN XiaoXiao,LIU ChaoJie,FAN ShuangXi. Effect of Exogenous Spermidine on Nitrogen Metabolism of Lettuce Under High-Temperature Stress [J]. Scientia Agricultura Sinica, 2022, 55(7): 1399-1410.
[3] WANG Juan,CHEN HaoNing,SHI DaChuan,YU TianYi,YAN CaiXia,SUN QuanXi,YUAN CuiLing,ZHAO XiaoBo,MOU YiFei,WANG Qi,LI ChunJuan,SHAN ShiHua. Functional Analysis of AhNRT2.7a in Response to Low-Nitrogen in Peanut [J]. Scientia Agricultura Sinica, 2022, 55(22): 4356-4372.
[4] ZHAO LiMing,HUANG AnQi,WANG YaXin,JIANG WenXin,ZHOU Hang,SHEN XueFeng,FENG NaiJie,ZHENG DianFeng. Effect of Deep Tillage Under Continuous Rotary Tillage on Yield Formation of High-Quality Japonica Rice in Cold Regions [J]. Scientia Agricultura Sinica, 2022, 55(22): 4550-4566.
[5] ZHU ChangWei,MENG WeiWei,SHI Ke,NIU RunZhi,JIANG GuiYing,SHEN FengMin,LIU Fang,LIU ShiLiang. The Characteristics of Soil Nutrients and Soil Enzyme Activities During Wheat Growth Stage Under Different Tillage Patterns [J]. Scientia Agricultura Sinica, 2022, 55(21): 4237-4251.
[6] RU Chen,HU XiaoTao,LÜ MengWei,CHEN DianYu,WANG WenE,SONG TianYuan. Effects of Nitrogen on Nitrogen Accumulation and Distribution, Nitrogen Metabolizing Enzymes, Protein Content, and Water and Nitrogen Use Efficiency in Winter Wheat Under Heat and Drought Stress After Anthesis [J]. Scientia Agricultura Sinica, 2022, 55(17): 3303-3320.
[7] ZHAO XiaoHui,ZHANG YanYan,RONG YaSi,DUAN JianZhao,HE Li,LIU WanDai,GUO TianCai,FENG Wei. Study on Critical Nitrogen Dilution Model of Winter Wheat Spike Organs Under Different Water and Nitrogen Conditions [J]. Scientia Agricultura Sinica, 2022, 55(17): 3321-3333.
[8] YUAN Cheng,ZHANG MingCong,WANG MengXue,HUANG BingLin,XIN MingQiang,YIN XiaoGang,HU GuoHua,ZHANG YuXian. Effects of Intertillage Time and Depth on Photosynthetic Characteristics and Yield Formation of Soybean [J]. Scientia Agricultura Sinica, 2022, 55(15): 2911-2926.
[9] BAI Fei,BAI GuiPing,WANG ChunYun,LI Zhen,GONG DePing,HUANG Wei,CHENG YuGui,WANG Bo,WANG Jing,XU ZhengHua,KUAI Jie,ZHOU GuangSheng. Effects of Tillage Depth and Shading on Root Growth and Nutrient Utilization of Rapeseed [J]. Scientia Agricultura Sinica, 2022, 55(14): 2726-2739.
[10] MA LiXiao,LI Jing,ZOU ZhiChao,CAI AnDong,ZHANG AiPing,LI GuiChun,DU ZhangLiu. Effects of No-Tillage and Straw Returning on Soil C-Cycling Enzyme Activities in China: Meta-Analysis [J]. Scientia Agricultura Sinica, 2021, 54(9): 1913-1925.
[11] HAN ZhanYu,WU ChunYan,XU YanQiu,HUANG FuDeng,XIONG YiQin,GUAN XianYue,ZHOU LuJian,PAN Gang,CHENG FangMin. Effects of High-Temperature at Filling Stage on Grain Storage Protein Accumulation and Its Biosynthesis Metabolism for Rice Plants Under Different Nitrogen Application Levels [J]. Scientia Agricultura Sinica, 2021, 54(7): 1439-1454.
[12] BiSheng WANG,WeiShui YU,XuePing WU,LiLi GAO,Jing LI,XiaoJun SONG,ShengPing LI,JinJing LU,FengJun ZHENG,DianXiong CAI. Effects of Straw Addition on Soil Organic Carbon and Related Factors Under Different Tillage Practices [J]. Scientia Agricultura Sinica, 2021, 54(6): 1176-1187.
[13] ZHENG FengJun, WANG Xue, LI ShengPing, LIU XiaoTong, LIU ZhiPing, LU JinJing, WU XuePing, XI JiLong, ZHANG JianCheng, LI YongShan. Synergistic Effects of Soil Moisture, Aggregate Stability and Organic Carbon Distribution on Wheat Yield Under No-Tillage Practice [J]. Scientia Agricultura Sinica, 2021, 54(3): 596-607.
[14] HUANG Ming,WU JinZhi,LI YouJun,FU GuoZhan,ZHAO KaiNan,ZHANG ZhenWang,YANG ZhongShuai,HOU YuanQuan. Effects of Tillage Practices and Nitrogen Fertilizer Application Rates on Grain Yield, Protein Content in Winter Wheat and Soil Nitrate Residue in Dryland [J]. Scientia Agricultura Sinica, 2021, 54(24): 5206-5219.
[15] ZHOU YongJie,XIE JunHong,LI LingLing,WANG LinLin,LUO ZhuZhu,WANG JinBin. Effects of Long-Term Reduce/Zero Tillage and Nitrogen Fertilizer Reducing on Maize Yield and Soil Carbon Emission Under Fully Plastic Mulched Ridge-Furrow Planting System [J]. Scientia Agricultura Sinica, 2021, 54(23): 5054-5067.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd