Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (12): 2346-2356.doi: 10.3864/j.issn.0578-1752.2012.12.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Epistatic Effects and QE Interaction Effects of QTLs for Two-Seed Pod Length and Width in Soybean

 YANG  Zhen, PEI  Yu-Feng, XIE  Sheng-Nan, LIU  Chun-Yan, JIANG  Hong-Wei, HAN  Xue, XIN  Da-Wei, CHEN  Qing-Shan, HU  Guo-Hua   

  1. 1.东北农业大学农学院，哈尔滨 150030
    2.黑龙江省农垦科研育种中心，哈尔滨 150090
    3.国家大豆工程技术研究中心，哈尔滨 150050
  • Received:2011-12-19 Online:2012-06-15 Published:2012-03-19

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Abstract: 【Objective】The objective of this study was to investigate the epistatic effects and QE interaction effects of QTLs for two-seed pod length and two-seed pod width.【Method】In order to find out these results, a F2:14-F2:18 RIL population containing 147 lines derived from a cross between Charleston as female parent and Dongnong594 as male parent were used in this experiment. A genetic linkage map was constructed with 164 SSR primers screened in two parents and amplified in 147 lines population. QTLs and epistatic effects and QE Interaction effects of QTLs were located on one site in five years with a mixed linear model (QTLmapper1.6) . QTLs for these were detected in a five-year experiment with the recombination inbred lines (RIL) population derived from a cross between Charleston and Dongnong 594. 【Result】Eight two-seed pod length QTLs and six two-seed pod width with additive effects were detected, the additive effects contribution rate were 27.2% and 16.27%, respectively, the general contribution of interaction between QTLs and environment were 10.19% and 12.18%. Nine pairs of two-seed pod length QTLs with epistatic effects were found in the RIL, accounting for 9.02% of the general phenotypic variation, and eight pairs of two-seed pod width QTLs accounting for 8.81% of the general phenotypic variation.【Conclusion】The results indicated that the epistatic effects and the environmental factor played an important role in the inheritance of two seed-pod length and width in soybean. Therefore, both major and minor QTLs should be considered in the improvement in soybean breeding.

Key words: soybean, two-seed pod length, two-seed pod width, mixed linear model, QTL ×, environment interaction, epistatic effects

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