Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (12): 2339-2345.doi: 10.3864/j.issn.0578-1752.2012.12.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Expression on Profiling of Rice PP2Ac Type Phosphatase Proteins in Seedlings Under Salt-stressed Conditions

 LIU  Zhao, JIA  Lin, JIA  Meng, GUAN  Ming-Li, CAO  Ying-Hao, LIU  Li-Juan, CAO  Zhen-Wei, LI  Li-Yun, LIU  Guo-Zhen   

  1. 1.河北农业大学生命科学学院，河北保定 071001
    2.中国科学院北京基因组研究所，北京 101300
  • Received:2012-01-04 Online:2012-06-15 Published:2012-02-20

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Abstract: 【Objective】The protein expression profiling and the mechanism of rice salt-tolerance were studied.【Method】 The expression patterns of five rice phospatase 2A catalytic subunits (PP2Ac) in seedlings under salt-stressed conditions were surveyed by Western blotting via antibody-based proteomics strategy. 【Result】 The results indicated that the expression of OsPP2Ac-4 protein was up-regulated in salt-tolerant rice variety Lansheng, and the expressions of OsPP2Ac-2, OsPP2Ac-3 and OsPP2Ac-5 were up-regulated in salt-sensitive rice variety 9311, while the expression of OsPP2Ac-4 was down-regulated in 9311. The expression of PP2Ac proteins was stable and in a similar level for Lansheng and 9311 under normal growth conditions, but the abundance variations were occurred only under salt-stressed conditions. Based on the Massively Parallel Signature Sequencing (MPSS) data derived from salt-stressed rice seedlings, it was found that the transcription of OsPP2Ac-2, OsPP2Ac-3 and OsPP2Ac-5 were down-regulated by comparison with control seedlings without salt treatment.【Conclusion】Four salt stress-related rice PP2Ac proteins were identified.

Key words: rice (Oryza sativa L.), PP2Ac phosphatase, salt stress, Western blotting

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