Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (11): 2273-2279.doi: 10.3864/j.issn.0578-1752.2012.11.017

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Genotype and Gene Expression of SCD1 Gene Associated with the Content and Composition of Fatty Acid in Milk

 LI  Yun-Long, YANG  Zhang-Ping, WANG  Xiao-Long, CHANG  Ling-Ling, JI  De-Jun, LIU  Shan, GAN  Zong-Hui   

  1. 扬州大学动物科学与技术学院，江苏扬州 225009
  • Received:2011-09-28 Online:2012-06-01 Published:2012-01-12

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Abstract: 【Objective】 The effect of SCD1 different genotypes on gene expression and milk fatty acid composition was studied. 【Method】 Three hundred and three Chinese Holstein cows of a dairy farm were used to detect the polymorphism of the exon 5 of SCD1 gene using PCR-SSCP, and the relative gene expression was assayed by quantitative FQ-PCR, the milk fatty acid composition was assayed using the method of gas chromatography.【Result】One SNP, C878T, was detected, defining three genotypes, CC, CT and TT, with frequencies of 0.617, 0.339 and 0.044, respectively. The allele frequencies of C and T were, respectively, 0.787 and 0.213. Only the expression of CT genotype was significantly higher than TT genotype (P＜0.05). Total saturated fatty acids of TT was significantly higher than CC or CT (P＜0.05). There were highly significant differences in the fatty acid C6:0, C8:0, C14:0, C15:0 and c9t11CLA (P＜0.01) , and significant differences in the fatty acid C4:0, C12:0, C17:0 among these three genotypes (P＜0.05).【Conclusion】The SNP C878T of SCD1 has a great genetic effect on the gene expression of SCD1 and the content of fatty acid in milk, it could be a useful genetic marker for milking trait in Chinese Holstein breeding.

Key words: Chinese Hosltein cow, SCD1 gene, genotype, milk, fatty acid

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