代乳粉添加甘露寡糖对7—28日龄湖羊羔羊胃肠道发育的影响

doi:10.3864/j.issn.0578-1752.2020.02.014

Abstract

Abstract:

【Objective】This study was conducted to investigate the effects of mannan oligosaccharides (MOS) supplementation to milk replacer on the development of gastrointestinal tract of 7-28 day-old Hu lambs. 【Method】 Thirty 7 day-old Hu male lambs were chosen and divided into 2 groups randomly, fifteen lambs in each group and each lamb as a repeat. Lambs were fed milk replacer with or without 0.2 % MOS, respectively. The test lasted for 21 days. Eight lambs were selected from each group randomly and slaughtered at 28 day-old. The weights of the compound stomach and the intestinal tract with and without content, and lengths of the intestinal tract were measured, and the relative quality and length were calculated. While the tissue samples from fundus gland region of the abomasum, the middle part of duodenum, jejunum and ileum were fixed in paraformaldehyde to analyse the histomorphology, and the apoptotic rate of intestinal epithelial cells as well. And the mRNA expression of claudin 1, zonula occludens-1 (ZO-1), and occludin protein of duodenum, jejunum and ileum mucosa were measured.【Result】The results showed that except relative length of jejunum (P=0.040), the relative weights (% body weight, % stomach weight, % intestinal tract weight, and % gastrointestinal tract weight), relative lengths (% intestinal tract length), content of stomach and intestinal tract (% body weight, % stomach content weight, % intestinal tract content weight, and % gastrointestinal tract content weight), the apoptotic rate of intestinal epithelial cells and mRNA expression of claudin 1 protein in intestinal tract of lambs were not affected by MOS (P＞0.05). However, MOS elongated the villus height and the muscular thickness, and decreased the villus width of lamb duodenum significantly (P=0.033, P=0.047, P=0.015). MOS also up-regulated the mRNA expression of ZO-1 protein of lamb jejunum significantly (P=0.028). And there was a tendency that MOS elongated villus height, width and crypt depth of ileum, muscular thickness of abomasum, and mRNA expression of occludin protein of ileum (P=0.075, P=0.078, P=0.085, P=0.084, P=0.052).【Conclusion】MOS almost did not affect the relative weights, lengths, and content distribution of gastrointestinal tract of 7-28 days old Hu lambs, but improved the histomorphology of duodenum and ileum, indicating it could maintain barrier function of intestinal tract and benefit to nutrients digestibility.

Key words: lamb, mannan oligosaccharides, milk replacer, gastrointestinal tract, development

ZHENG Chen,LI FaDi,LI Fei,ZHOU JuWang,DUAN PengWei,LIU HuiHui,FAN HaiMiao,ZHU WeiLi,LIU Ting. Effects of Adding Mannan Oligosaccharides to Milk Replacer on the Development of Gastrointestinal Tract of 7-28 Days Old Hu Lambs[J].Scientia Agricultura Sinica, 2020, 53(2): 398-408.

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Figures/Tables 8

Table 1

Table 2

Table 3

Effects of MOS on relative quality of gastrointestinal tract of lambs"

项目Item		对照组Control	MOS组 MOS	SEM	P值 P value
胃室 Stomach
瘤胃 Rumen	%全胃质量 % the quality of total stomach	29.70	30.45	0.58	0.533
瘤胃 Rumen	%全胃肠质量 % the quality of total gastrointestinal tract	6.71	6.62	0.27	0.869
网胃 Reticulum	%全胃质量 % the quality of total stomach	9.16	9.46	0.40	0.724
网胃 Reticulum	%全胃肠质量 % the quality of total gastrointestinal tract	2.05	2.06	0.10	0.939
瓣胃 Omasum	%全胃质量 % the quality of total stomach	4.08	4.15	0.21	0.883
瓣胃 Omasum	%全胃肠质量 % the quality of total gastrointestinal tract	0.92	0.89	0.05	0.795
皱胃 Abomasum	%全胃质量 % the quality of total stomach	57.05	55.94	0.74	0.471
皱胃 Abomasum	%全胃肠质量 % the quality of total gastrointestinal tract	12.81	12.14	0.35	0.356
肠道 Intestinal tract
十二指肠 Duodenum	%全肠质量 % the quality of total intestinal tract	3.22	2.93	0.14	0.297
十二指肠 Duodenum	%全胃肠质量 % the quality of total gastrointestinal tract	2.49	2.28	0.09	0.292
空肠Jejunum	%全肠质量 % the quality of total intestinal tract	64.21	61.69	1.07	0.254
空肠Jejunum	%全胃肠质量 % the quality of total gastrointestinal tract	49.80	48.36	1.06	0.517
回肠 Ileum	%全肠质量 % the quality of total intestinal tract	1.91	2.41	0.21	0.258
回肠 Ileum	%全胃肠质量 % the quality of total gastrointestinal tract	1.69	1.88	0.13	0.477
盲肠 Cecum	%全肠质量 % the quality of total intestinal tract	5.57	6.89	0.44	0.135
盲肠 Cecum	%全胃肠质量 % the quality of total gastrointestinal tract	4.32	5.38	0.34	0.116
结肠 Colon	%全肠质量 % the quality of total intestinal tract	18.95	19.43	0.57	0.686
结肠 Colon	%全胃肠质量 % the quality of total gastrointestinal tract	14.67	15.17	0.41	0.558
直肠 Rectum	%全肠质量 % the quality of total intestinal tract	6.14	6.65	0.23	0.275
直肠 Rectum	%全胃肠质量 % the quality of total gastrointestinal tract	4.75	5.19	0.16	0.162

Table 3

Table 4

Table 5

Table 6

Effects of MOS on relative quality of each part of stomach and intestinal content to total stomach and intestinal tract content, and gastrointestinal tract content of lambs"

项目 Item		对照组Control	MOS组 MOS	SEM	P值 P value
胃室内容物 Stomach content
瘤胃内容物 Rumen content	%全胃内容物质量 % the quality of total stomach content	26.96	33.15	2.56	0.239
瘤胃内容物 Rumen content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	17.30	21.12	1.80	0.306
网胃内容物 Reticulum content	%全胃内容物质量 % the quality of total stomach content	4.99	6.68	1.28	0.532
网胃内容物 Reticulum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	3.50	4.06	0.03	0.742
瓣胃内容物 Omasum content	%全胃内容物质量 % the quality of total stomach content	0.23	0.24	0.03	0.988
瓣胃内容物 Omasum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	0.14	0.13	0.01	0.698
皱胃内容物 Abomasum content	%全胃内容物质量 % the quality of total stomach content	68.58	60.09	2.71	0.119
皱胃内容物 Abomasum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	43.14	39.30	2.35	0.434
肠道内容物 Intestinal tract content
十二指肠内容物 Duodenum content	%全肠内容物质量 % the quality of total intestinal tract content	7.20	6.23	1.63	0.777
十二指肠内容物 Duodenum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	1.98	2.22	0.39	0.765
空肠内容物 Jejunum content	%全肠内容物质量 % the quality of total intestinal tract content	39.34	46.21	2.22	0.126
空肠内容物 Jejunum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	14.28	16.95	1.69	0.451
回肠内容物 Ileum content	%全肠内容物质量 % the quality of total intestinal tract content	1.75	1.45	0.31	0.666
回肠内容物 Ileum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	0.80	0.57	0.20	0.618
盲肠内容物 Cecum content	%全肠内容物质量 % the quality of total intestinal tract content	12.73	9.88	1.35	0.312
盲肠内容物 Cecum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	4.88	3.51	0.70	0.345
结肠内容物 Colon content	%全肠内容物质量 % the quality of total intestinal tract content	33.02	30.57	2.83	0.681
结肠内容物 Colon content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	12.49	10.25	1.17	0.356
直肠内容物 Rectum content	%全肠内容物质量 % the quality of total intestinal tract content	5.99	7.80	1.05	0.405
直肠内容物 Rectum content	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	2.06	2.77	0.42	0.414

Table 6

Table 7

Table 8

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营养成分 Ingredients^1}	含量 Concentration
干物质 Dry matter	95.68
蛋白质 Protein	24.89
粗脂肪 Ether extract	17.50
粗纤维 Crude fiber	2.16
粗灰分 Crude ash	5.81
钙 Calcium	1.02
总磷 Total phosphorus	0.59

项目Item	对照组Control	MOS组 MOS	SEM	P值 P value
皱胃 Abomasum
黏膜厚度 Mucosa thickness	333.36	331.46	9.74	0.927
肌层厚度 Muscular thickness	548.48	686.99	40.11	0.084
十二指肠 Duodenum
绒毛高度 Villus height	361.99b	446.39a	20.44	0.033
绒毛宽度 Villus width	80.04a	61.96b	3.92	0.015
隐窝深度 Crypt depth	148.87	163.33	8.46	0.414
肌层厚度 Muscular thickness	161.93b	201.87a	10.25	0.047
绒毛高度/隐窝深度值V/C	2.57	2.82	0.11	0.258
空肠Jejunum
绒毛高度 Villus height	431.59	445.30	20.29	0.748
绒毛宽度 Villus width	71.13	68.01	2.04	0.464
隐窝深度 Crypt depth	173.48	158.21	6.65	0.265
肌层厚度 Muscular thickness	157.73	153.01	8.27	0.786
绒毛高度/隐窝深度值V/C	2.59	2.89	0.09	0.118
回肠 Ileum
绒毛高度 Villus height	373.81	428.38	15.25	0.075
绒毛宽度 Villus width	68.91	78.87	2.81	0.078
隐窝深度 Crypt depth	176.96	213.10	10.38	0.085
肌层厚度 Muscular thickness	274.31	230.82	18.54	0.267
绒毛高度/隐窝深度值V/C	2.19	2.15	0.06	0.775

项目Item	对照组Control	MOS组 MOS	SEM	P值 P value
十二指肠 Duodenum
Claudin 1	0.98	0.94	0.10	0.863
ZO-1	0.92	1.05	0.08	0.403
Occludin	0.86	1.18	0.10	0.112
细胞凋亡 Apoptosis (%)	11.27	5.04	2.90	0.302
空肠Jejunum
Claudin 1	0.91	1.09	0.21	0.671
ZO-1	0.80b	1.20a	0.10	0.028
Occludin	0.96	1.04	0.08	0.630
细胞凋亡 Apoptosis (%)	21.09	18.08	5.40	0.791
回肠 Ileum
Claudin 1	0.87	0.81	0.18	0.875
ZO-1	0.92	1.06	0.07	0.362
Occludin	0.83	1.17	0.09	0.052
细胞凋亡 Apoptosis (%)	47.29	37.62	6.54	0.487

Effects of Adding Mannan Oligosaccharides to Milk Replacer on the Development of Gastrointestinal Tract of 7-28 Days Old Hu Lambs

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项目Item	对照组Control	MOS组 MOS	SEM	P值 P value
胃室 Stomach
瘤胃 Rumen	0.54	0.50	0.02	0.386
网胃 Reticulum	0.16	0.15	0.01	0.555
瓣胃 Omasum	0.08	0.07	0.01	0.488
皱胃 Abomasum	1.04	0.92	0.04	0.138
肠道 Intestinal tract
十二指肠 Duodenum	0.20	0.17	0.01	0.066
空肠Jejunum	4.00	3.71	0.14	0.316
回肠 Ileum	0.13	0.14	0.01	0.697
盲肠 Cecum	0.35	0.41	0.02	0.201
结肠 Colon	1.18	1.15	0.04	0.675
直肠 Rectum	0.38	0.39	0.01	0.663

项目Item	对照组Control	MOS组 MOS	SEM	P值 P value
十二指肠 Duodenum	2.29	2.01	0.09	0.107
空肠Jejunum	81.84a	80.53b	0.33	0.040
回肠 Ileum	0.83	0.95	0.01	0.292
盲肠 Cecum	0.91	1.12	0.06	0.094
结肠 Colon	11.96	12.89	0.29	0.115
直肠 Rectum	2.17	2.49	0.11	0.151

项目Item	对照组Control	MOS组 MOS	SEM	P值 P value
胃室内容物 Stomach content
瘤胃内容物 Rumen content	1.44	1.67	0.20	0.587
网胃内容物 Reticulum content	0.24	0.34	0.07	0.473
瓣胃内容物 Omasum content	0.01	0.01	0.001	0.946
皱胃内容物 Abomasum content	3.43	3.18	0.24	0.623
肠道内容物 Intestinal tract content
十二指肠内容物 Duodenum content	0.15	0.14	0.02	0.868
空肠内容物Jejunum content	1.26	1.35	0.20	0.833
回肠内容物 Ileum content	0.11	0.05	0.03	0.378
盲肠内容物 Cecum content	0.43	0.30	0.08	0.422
结肠内容物 Colon content	1.08	0.81	0.13	0.298
直肠内容物 Rectum content	0.17	0.21	0.03	0.503

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