Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (4): 726-733.doi: 10.3864/j.issn.0578-1752.2012.04.013

• HORTICULTURE • Previous Articles     Next Articles

Analysis of Genetic Diversity and Construction of Fingerprint of Vriesea by ISSR

 GE  Ya-Ying, ZHANG  , FEI  , SHEN  Xiao-Lan, LIU  Jian-Xin, WANG  Wei-Yong, TIAN  Dan-Qing, ZHANG  , ZHI    

  1. 1.浙江省农业科学院花卉研究开发中心，杭州 311202
  • Received:2011-08-23 Online:2012-02-15 Published:2011-12-01

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Abstract: 【Objective】 The purpose of this experiment is to study the genetic diversity and phylogenetic relationship of Vriesea cultivars and establish their DNA fingerprints, which will provide some guidance for crossbreeding and cultivar identification of ornamental bromeliads in Vriesea. 【Method】 Inter-simple sequence repeat（ISSR）was conducted to assess the genetic diversity of 41 Vriesea cultivars and the DNA fingerprints of the cultivars were established.【Result】Among the 41 Vriesea cultivars, 12 ISSR primers produced 132 loci, of which 125 were polymorphisms, and the percentage of polymorphic loci was up to 94.70%. The average value of the effective number of alleles, Nei’s genetic diversity and Shannon’s information index was 1.5311, 0.3101 and 0.4668, respectively. The similarity coefficient between the 41 Vriesea cultivars ranged from 0.3561 to 0.9394. Based on the unweighted pari group method with arithmetic average (UPGMA) and ISSR molecular markers, the 41 Vriesea cultivars were divided into two groups at the similarity coefficient of 0.601. In addition, the DNA fingerprints for the Vriesea cultivars were established by 4 polymorphic ISSR primers.【Conclusion】There existed abundant genetic diversity among 41 Vriesea cultivars and their relationships were significantly correlated with leaf stripe. ISSR molecular markers could be effectively used in genetic diversity and fingerprint analysis for Vriesea cultivars.

Key words: Vriesea, ISSR, genetic diversity, fingerprint

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