Scientia Agricultura Sinica ›› 2024, Vol. 57 ›› Issue (6): 1180-1190.doi: 10.3864/j.issn.0578-1752.2024.06.012

• FOOD SCIENCE AND ENGINEERING • Previous Articles     Next Articles

Analysis of the Effect of Dielectric Barrier Discharge Cold Plasma on Phenolic Metabolism of Stored Paddy Rice Under High Temperature Stress

HOU Shuai1(), ZHANG YiJia1, ZHOU DanDan2, MA FeiYang1, WANG DaPeng1, ZHAO SiQi1, DING Chao1, LIU Qiang1()   

  1. 1 College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety/Jiangsu Key Laboratory of Quality Control and Further Processing of Cereals and Oil, Nanjing 210023
    2 College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037
  • Received:2023-10-07 Accepted:2024-01-24 Online:2024-03-25 Published:2024-03-25
  • Contact: LIU Qiang

RichHTML

4

PDF

63

Abstract:

【Objective】 The aim of his study was to explore the impact of dielectric barrier discharge cold plasma (DBD-CP) treatment on the phenolic metabolism of stored rice under high-temperature stress.【Method】 Under simulated summer high- temperature conditions (35 ℃), fresh high-moisture rice (Japonica rice, 16.0% moisture content on a wet basis) was used as the raw material, the effects of DBD-CP treatment on quality parameters such as L*, a*, b*, yellowness index (YI), reactive oxygen species, and malondialdehyde (MDA) in stored rice were analyzed. Additionally, the study aims to analyze the changes in total phenols, total flavonoids, and antioxidant activities during the storage process. The investigation would involve multiple validations, including ABTS+ and DPPH radical scavenging activities, ferric ion reducing power, and the activity changes of key rate-limiting enzymes in phenylpropane metabolism. The ultimate goal was to explore the impact of DBD-CP treatment on the phenolic metabolism of stored rice. 【Result】 After DBD-CP treatment, the color stability of rice during storage significantly improved. The yellowing index of the treatment group decreased significantly by 12.6% after 60 days of storage. After 20 days of storage, the levels of superoxide anion, hydrogen peroxide, and MDA decreased by 14.8%, 41.6%, and 21.6%, respectively, and the deterioration rate of fresh high-moisture rice was significantly inhibited. Additionally, during the high-temperature stress storage period, the total phenol and total flavonoid content in the treatment group increased simultaneously (P<0.05), with maximum increases of 1.23 and 1.34 times, respectively. The antioxidant properties and iron ion reduction capacity of the corresponding samples were also improved (P<0.05). Combined with the analysis of the activity of key limiting enzymes in phenylpropane metabolism, the activities of phenylalanine ammonia-lyase (PAL), cinnamic acid-4-hydroxylase (C4H), and chalcone synthase (CHS) were significantly enhanced, with increases ranging from 1.71 to 2.28 times. Further correlation analysis confirmed the close association (P<0.05) between the content of phenolic substances in high-moisture rice under high-temperature stress and the activities of PAL, C4H, and CHS enzymes, and a significant negative correlation (P<0.05) with internal reactive oxygen species and MDA content. 【Conclusion】 Dielectric barrier discharge cold plasma treatment could promote the synthesis rate of phenolic compounds within the grains of high-moisture rice by activating phenylpropane metabolism. This treatment enhanced the tissue's antioxidant properties, alleviated membrane lipid peroxidation, and reduced the accumulation of free radicals. As a result, it delayed the quality deterioration of rice under high-temperature storage conditions.

Key words: DBD-CP, phenylpropanoid metabolism, phenolic compounds, antioxidant activity, storage

Fig. 1

The effect of DBD-CP treatment on the apparent color of rice during storage *: Significant difference (P<0.05); **: Extremely significant difference (P<0.01). The same as below"

Fig. 2

Effect of DBD-CP treatment on the content of total phenols and total flavonoids of rice during storage"

Fig. 3

Effect of DBD-CP treatment on the oxidation resistance of rice during storage"

Fig. 4

Effect of DBD-CP treatment on active oxygen content in rice grain during storage"

Fig. 5

Effect of DBD-CP treatment on MDA content in rice"

Fig. 6

Effect of DBD-CP treatment on the activities of key enzymes involved in phenylpropanoid metabolism in rice"

Fig. 7

Correlation analysis between phenylpropanoid metabolism and phenolic compounds in rice treated with DBD-CP"

[1]
程晓雨, 盛亚男, 冯玉超, 王长远. 储藏对稻谷代谢产物的影响研究. 中国粮油学报, 2023. doi: 10.20048/j.cnki.issn.1003-0174.000573.
CHENG X Y, SHENG Y N, FENG Y C, WANG C Y. Study on the effect of storage on rice metabolites. Journal of the Chinese Cereals and Oil Association, 2023. doi: 10.20048/j.cnki.issn.1003-0174.000573. (in Chinese)
[2]
TONG C, GAO H Y, LUO S J, LIU L, BAO J S. Impact of postharvest operations on rice grain quality: A review. Comprehensive Reviews in Food Science and Food Safety, 2019, 18(3): 626-640.

doi: 10.1111/1541-4337.12439
[3]
吴佳. 吉林省优质稻谷产后损失评价与管理策略研究[D]. 长春: 吉林大学, 2021.
WU J. Study on postpartum loss evaluation and management strategy of high quality rice in Jilin province[D]. Changchun: Jilin University, 2021. (in Chinese)
[4]
刘强, 刘纪伟, 田恬, 严薇, 刘兵, 赵思琪, 胡秋辉, 丁超. 高温胁迫下糙米短期储藏气味指纹图谱变化规律的动态分析. 中国农业科学, 2021, 54(2): 379-391. doi: 10.3864/j.issn.0578-1752.2021.02. 013.
LIU Q, LIU J W, TIAN T, YAN W, LIU B, ZHAO S Q, HU Q H, DING C. Dynamic analysis for the characteristics of flavor fingerprints for brown rice in short-term storage under high temperature stress. Scientia Agricultura Sinica, 2021, 54(2): 379-391. doi: 10.3864/j.issn.0578-1752.2021.02.013. (in Chinese)
[5]
HAN Y X, CHENG J H, SUN D W. Activities and conformation changes of food enzymes induced by cold plasma: a review. Critical Reviews in Food Science and Nutrition, 2019, 59(5): 794-811.

doi: 10.1080/10408398.2018.1555131 pmid: 30799633
[6]
CHEN Y Q, CHENG J H, SUN D W. Chemical, physical and physiological quality attributes of fruit and vegetables induced by cold plasma treatment: Mechanisms and application advances. Critical Reviews in Food Science and Nutrition, 2020, 60(16): 2676-2690.

doi: 10.1080/10408398.2019.1654429
[7]
GIANNOGLOU M, XANTHOU Z M, CHANIOTI S, STERGIOU P, CHRISTOPOULOS M, DIMITRAKELLIS P, EFTHIMIADOU Α, GOGOLIDES Ε, KATSAROS G. Effect of cold atmospheric plasma and pulsed electromagnetic fields on strawberry quality and shelf-life. Innovative Food Science & Emerging Technologies, 2021, 68: 102631.
[8]
ZHOU Y H, VIDYARTHI S K, ZHONG C S, ZHENG Z A, AN Y, WANG J, WEI Q, XIAO H W. Cold plasma enhances drying and color, rehydration ratio and polyphenols of wolfberry via microstructure and ultrastructure alteration. LWT, 2020, 134: 110173.

doi: 10.1016/j.lwt.2020.110173
[9]
FARIAS T R B, RODRIGUES S, FERNANDES F A N. Effect of dielectric barrier discharge plasma excitation frequency on the enzymatic activity, antioxidant capacity and phenolic content of apple cubes and apple juice. Food Research International, 2020, 136: 109617.

doi: 10.1016/j.foodres.2020.109617
[10]
DRAGOVIĆ-UZELAC V, BRALA A, LEVAJ B, KOVAČEVIĆ D B, SAVIĆ Z, BIŠKO A. Evaluation of phenolic content and antioxidant capacity of blueberry cultivars (Vaccinium corymbosum L.) grown in the northwest Croatia. Food Technology and Biotechnology, 2010, 48(2): 214-221.
[11]
ZHOU D D, SUN R, ZHU W X, SHI Y L, NI S J, WU C E, LI T T. Impact of dielectric barrier discharge cold plasma on the quality and phenolic metabolism in blueberries based on metabonomic analysis. Postharvest Biology and Technology, 2023, 197: 112208.

doi: 10.1016/j.postharvbio.2022.112208
[12]
MISRA N N, MOISEEV T, PATIL S, PANKAJ S K, BOURKE P, MOSNIER J P, KEENER K M, CULLEN P J. Cold plasma in modified atmospheres for post-harvest treatment of strawberries. Food and Bioprocess Technology, 2014, 7(10): 3045-3054.

doi: 10.1007/s11947-014-1356-0
[13]
LIU S Y, HE T, RAFIQUE H, ZOU L, HU X Z. Effect of low-temperature plasma treatment on the microbial inactivation and physicochemical properties of the oat grain. Cereal Chemistry, 2022, 99(6): 1373-1382.

doi: 10.1002/cche.v99.6
[14]
YAN W, LIU Q, WANG Y, TAO T T, LIU B, LIU J, DING C. Inhibition of lipid and aroma deterioration in rice bran by infrared heating. Food and Bioprocess Technology, 2020, 13(10): 1677-1687.

doi: 10.1007/s11947-020-02503-z
[15]
ZHANG M W, ZHANG R F, ZHANG F X, LIU R H. Phenolic profiles and antioxidant activity of black rice bran of different commercially available varieties. Journal of Agricultural and Food Chemistry, 2010, 58(13): 7580-7587.

doi: 10.1021/jf1007665 pmid: 20521821
[16]
李青, 张名位, 张瑞芬, 魏振承, 邓媛元, 唐小俊, 张业辉, 李武, 马永轩. 5种籼稻品种谷壳中游离态和结合态酚类物质含量及其抗氧化活性比较. 中国农业科学, 2012, 45(6): 1150-1158. doi: 10.3864/j.issn.0578-1752.2012.06.013.
LI Q, ZHANG M W, ZHANG R F, WEI Z C, DENG Y Y, TANG X J, ZHANG Y H, LI W, MA Y X. Free and bound phenolic contents and antioxidant activity of five varieties of indica rice husk. Scientia Agricultura Sinica, 2012, 45(6): 1150-1158. doi: 10.3864/j.issn.0578-1752.2012.06.013. (in Chinese)
[17]
SINGLETON V L, ORTHOFER R, LAMUELA-RAVENTÓS R M. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Oxidants and Antioxidants Part A. Amsterdam: Elsevier, 1999: 152-178.
[18]
JIA Z S, TANG M C, WU J M. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 1999, 64(4): 555-559.

doi: 10.1016/S0308-8146(98)00102-2
[19]
BENZIE I F, STRAIN J J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 1996, 239(1): 70-76.

doi: 10.1006/abio.1996.0292
[20]
滕慧, 米亚妮, 何远菊, 邓宏挺, 艾超, 陈雷. 酰基稳态化花色苷的制备及其热降解特性和抗氧化活性. 食品科学, 2023, 44(6): 49-56.
TENG H, MI Y N, HE Y J, DENG H T, AI C, CHEN L. Preparation of acylated anthocyanin and its thermal degradation properties and antioxidant activity. Food Science, 2023, 44(6): 49-56. (in Chinese)

doi: 10.7506/spkx1002-6630-20220705-044
[21]
HUANG H, BELWAL T, LI L, WANG Y, AALIM H, LUO Z S. Effect of modified atmosphere packaging of different oxygen levels on cooking qualities and phytochemicals of brown rice during accelerated aging storage at 37 ℃. Food Packaging and Shelf Life, 2020, 25: 100529.

doi: 10.1016/j.fpsl.2020.100529
[22]
LIU Y Q, LIU J G, WANG A Q, WANG R, SUN H, STRAPPE P, ZHOU Z K. Physiological and proteomic analyses provide insights into the rice yellowing. Journal of Cereal Science, 2020, 95: 103048.

doi: 10.1016/j.jcs.2020.103048
[23]
LIU J G, LIU M, LIU Y Q, JIA M, WANG S M, KANG X D, SUN H, STRAPPE P, ZHOU Z K. Moisture content is a key factor responsible for inducing rice yellowing. Journal of Cereal Science, 2020, 94: 102988.

doi: 10.1016/j.jcs.2020.102988
[24]
LIU Y Q, SHAD Z M, STRAPPE P, XU L H, ZHANG F J, CHEN Y L, LI D P. A review on rice yellowing: Physicochemical properties, affecting factors, and mechanism. Food Chemistry, 2022, 370: 131265.

doi: 10.1016/j.foodchem.2021.131265
[25]
PARK C E, KIM Y S, PARK K J, KIM B K. Changes in physicochemical characteristics of rice during storage at different temperatures. Journal of Stored Products Research, 2012, 48: 25-29.

doi: 10.1016/j.jspr.2011.08.005
[26]
XU C, ZHANG X Y, LIANG J, FU Y J, WANG J, JIANG M, PAN L. Cell wall and reactive oxygen metabolism responses of strawberry fruit during storage to low voltage electrostatic field treatment. Postharvest Biology and Technology, 2022, 192: 112017.

doi: 10.1016/j.postharvbio.2022.112017
[27]
YANG M Y, BAN Z J, LUO Z S, LI J H, LU H Y, LI D, CHEN C K, LI L. Impact of elevated O2 and CO2 atmospheres on chemical attributes and quality of strawberry (Fragaria × ananassa Duch.) during storage. Food Chemistry, 2020, 307: 125550.

doi: 10.1016/j.foodchem.2019.125550
[28]
ZHAO R P, HAO J X, XUE J, LIU H J, LI L T. Effect of high-voltage electrostatic field pretreatment on the antioxidant system in stored green mature tomatoes. Journal of the Science of Food and Agriculture, 2011, 91(9): 1680-1686.

doi: 10.1002/jsfa.4369 pmid: 21480264
[29]
JIANG Y L, YU L, HU Y W, ZHU Z C, ZHUANG C J, ZHAO Y Y, ZHONG Y. The preservation performance of chitosan coating with different molecular weight on strawberry using electrostatic spraying technique. International Journal of Biological Macromolecules, 2020, 151: 278-285.

doi: S0141-8130(20)30465-7 pmid: 32081757
[30]
LI D, LIMWACHIRANON J, LI L, DU R X, LUO Z S. Involvement of energy metabolism to chilling tolerance induced by hydrogen sulfide in cold-stored banana fruit. Food Chemistry, 2016, 208: 272-278.

doi: 10.1016/j.foodchem.2016.03.113 pmid: 27132850
[31]
严薇. 红外辐射对储藏稻谷脂质代谢的影响研究[D]. 南京: 南京财经大学, 2020.
YAN W. Effect of infrared radiation on lipid metabolism of stored rice[D]. Nanjing: Nanjing University of Finance & Economics, 2020. (in Chinese)
[32]
SHU P, MIN D D, AI W, LI J Z, ZHOU J X, LI Z L, ZHANG X H, SHI Z D, SUN Y J, JIANG Y P, LI F J, LI X A, GUO Y Y. L-Arginine treatment attenuates postharvest decay and maintains quality of strawberry fruit by promoting nitric oxide synthase pathway. Postharvest Biology and Technology, 2020, 168: 111253.

doi: 10.1016/j.postharvbio.2020.111253
[33]
ZHANG X, YANG Q X, LUO M L, SONG M, ZHOU Q, CHEN J Y, JI S J. Insights into profiling of p-coumaric acid treatment on delaying the yellowing of broccoli. Postharvest Biology and Technology, 2023, 201: 112371.

doi: 10.1016/j.postharvbio.2023.112371
[34]
LUO Z S, LI D D, DU R X, MOU W S. Hydrogen sulfide alleviates chilling injury of banana fruit by enhanced antioxidant system and proline content. Scientia Horticulturae, 2015, 183: 144-151.

doi: 10.1016/j.scienta.2014.12.021
[35]
CONCELLÓN A, ZARO M J, CHAVES A R, VICENTE A R. Changes in quality and phenolic antioxidants in dark purple American eggplant (Solanum melongena L. cv. Lucía) as affected by storage at 0℃ and 10℃. Postharvest Biology and Technology, 2012, 66: 35-41.

doi: 10.1016/j.postharvbio.2011.12.003
[36]
JAAKOLA L. New insights into the regulation of anthocyanin biosynthesis in fruits. Trends in Plant Science, 2013, 18(9): 477-483.

doi: 10.1016/j.tplants.2013.06.003 pmid: 23870661
[37]
CÖMERT E D, MOGOL B A, GÖKMEN V. Relationship between color and antioxidant capacity of fruits and vegetables. Current Research in Food Science, 2020, 2: 1-10.

doi: 10.1016/j.crfs.2019.11.001 pmid: 32914105
[38]
LI M L, LI X A, HAN C, JI N N, JIN P, ZHENG Y H. Physiological and metabolomic analysis of cold plasma treated fresh-cut strawberries. Journal of Agricultural and Food Chemistry, 2019, 67(14): 4043-4053.

doi: 10.1021/acs.jafc.9b00656 pmid: 30883111
[39]
HUA X W, LI T T, WU C E, ZHOU D D, FAN G J, LI X J, CONG K P, YAN Z C, WU Z H. Novel physical treatments (Pulsed light and cold plasma) improve the quality of postharvest apricots after long-distance simulated transportation. Postharvest Biology and Technology, 2022, 194: 112098.

doi: 10.1016/j.postharvbio.2022.112098
[1] ZHANG ZhiPeng, TAN YunXiu, LI BaoJun, LI YongCai, BI Yang, LI ShouQiang, WANG XiaoJing, ZHANG Yu, HU Dan. Effects of Exogenous Abscisic Acid Treatment on Periderm Suberification of Postharvest Mini-Tuber Potato from Aeroponic System and Its Possible Mechanisms [J]. Scientia Agricultura Sinica, 2023, 56(6): 1154-1167.
[2] GUO RongBo, LI GuoDong, PAN MengYu, ZHENG XianFeng, WANG ZhaoHui, HE Gang. Effects of Long-Term Straw Return and Nitrogen Application Rate on Organic Carbon Storage, Components and Aggregates in Cultivated Layers [J]. Scientia Agricultura Sinica, 2023, 56(20): 4035-4048.
[3] SONG ZhiZhong, WANG JianPing, SHI ShengPeng, CAO JingWen, LIU WanHao, XU WeiHua, XIAO HuiLin, TANG MeiLing. Identification and Cloning of Ferritin Family Genes in Grape and Response to Compound Amino Acid-Iron Spraying During Different Fruit Developmental Stages [J]. Scientia Agricultura Sinica, 2023, 56(18): 3629-3641.
[4] LÜ XinNing,WANG Yue,JIA RunPu,WANG ShengNan,YAO YuXin. Effects of Melatonin Treatment on Quality of Stored Shine Muscat Grapes Under Different Storage Temperatures [J]. Scientia Agricultura Sinica, 2022, 55(7): 1411-1422.
[5] PENG Xue,GAO YueXia,ZHANG LinXuan,GAO ZhiQiang,REN YaMei. Effects of High-Energy Electron Beam Irradiation on Potato Storage Quality and Bud Eye Cell Ultrastructure [J]. Scientia Agricultura Sinica, 2022, 55(7): 1423-1432.
[6] DONG FuCheng,MA ShuLi,SHI JuanJuan,ZHANG JunMei,CUI Yan,REN YouShe,ZHANG ChunXiang. Expression and Localization of LCN5 in Ram Reproductive Organs and Spermatozoa [J]. Scientia Agricultura Sinica, 2022, 55(7): 1445-1457.
[7] XIANG MiaoLian, WU Fan, LI ShuCheng, WANG YinBao, XIAO LiuHua, PENG WenWen, CHEN JinYin, CHEN Ming. Effects of Melatonin Treatment on Resistance to Black Spot and Postharvest Storage Quality of Pear Fruit [J]. Scientia Agricultura Sinica, 2022, 55(4): 785-795.
[8] ZOU YunQian,LIN ZiZhen,XU RangWei,CHENG YunJiang. Development and Evaluation of a Coating Substitute for Individual Polyethylene Film Packaging of Citrus Fruit [J]. Scientia Agricultura Sinica, 2022, 55(12): 2398-2412.
[9] JIN MengJiao,LIU Bo,WANG KangKang,ZHANG GuangZhong,QIAN WanQiang,WAN FangHao. Light Energy Utilization and Response of Chlorophyll Synthesis Under Different Light Intensities in Mikania micrantha [J]. Scientia Agricultura Sinica, 2022, 55(12): 2347-2359.
[10] HU FeiFei,QIAN ShuYi,HUANG Feng,JIANG Wei,QIANG Yu,JIANG Feng,HU HaiMei,LI Xia,ZHANG ChunHui. Effect of Low Voltage Electrostatic Field-Assisted Short-Term Frozen Storage on Quality of Pork [J]. Scientia Agricultura Sinica, 2021, 54(9): 1993-2005.
[11] HAN ZhanYu,WU ChunYan,XU YanQiu,HUANG FuDeng,XIONG YiQin,GUAN XianYue,ZHOU LuJian,PAN Gang,CHENG FangMin. Effects of High-Temperature at Filling Stage on Grain Storage Protein Accumulation and Its Biosynthesis Metabolism for Rice Plants Under Different Nitrogen Application Levels [J]. Scientia Agricultura Sinica, 2021, 54(7): 1439-1454.
[12] ZHENG FengJun, WANG Xue, LI ShengPing, LIU XiaoTong, LIU ZhiPing, LU JinJing, WU XuePing, XI JiLong, ZHANG JianCheng, LI YongShan. Synergistic Effects of Soil Moisture, Aggregate Stability and Organic Carbon Distribution on Wheat Yield Under No-Tillage Practice [J]. Scientia Agricultura Sinica, 2021, 54(3): 596-607.
[13] HOU ChengLi,HUANG CaiYan,ZHENG XiaoChun,LIU WeiHua,YANG Qi,ZHANG DeQuan. Changes of Antioxidant Activity and Its Possible Mechanism in Tan Sheep Meat in Different Postmortem Time [J]. Scientia Agricultura Sinica, 2021, 54(23): 5110-5124.
[14] ZHAO Shan,ZHONG LingLi,QIN Lin,HUANG ShiQun,LI Xi,ZHENG XingGuo,LEI XinYu,LEI ShaoRong,GUO LingAn,FENG JunYan. Effects of Different Drying Methods on Functional Components and Antioxidant Activity in Sweet Potato Leaves [J]. Scientia Agricultura Sinica, 2021, 54(21): 4650-4663.
[15] LIU Lian,TANG ZhiPeng,LI FeiFei,XIONG Jiang,LÜ BiWen,MA XiaoChuan,TANG ChaoLan,LI ZeHang,ZHOU Tie,SHENG Ling,LU XiaoPeng. Fruit Quality in Storage, Storability and Peel Transcriptome Analysis of Rong’an Kumquat, Huapi Kumquat and Cuimi Kumquat [J]. Scientia Agricultura Sinica, 2021, 54(20): 4421-4433.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd