乳酸菌半固态发酵对沙田柚果粉苦味及活性成分的影响

doi:10.3864/j.issn.0578-1752.2024.13.013

Abstract

Abstract:

【Objective】 The effects of semi-solid fermentation with different lactobacillus on the bitterness and active components in Shatianyu (Citrus grandis L. Osbeck) fruit powder (SFP) were explored. The aim of the present study was to establish the debitterizing technology and prepare low-bitterness SFP enriched in flavonoids and dietary fiber (DF), for providing the theoretical basis and technical support for the application of SFP in healthy food.【Method】 Shatianyu septum and pulp were dried and crushed to generate SFP, then, the bitterness of SFP and the equivalent main flavonoid glycosides (naringin and melitidin) in SPF were evaluated by sensory evaluation to clarify the bitter substance base. Lactobacillus plantarum GIM 1.1516 (LP1.1516), Lactobacillus casei GIM 1.411 (LC1.411), Lactobacillus acidophilus GIM 1.731 (LA1.731) and Lactobacillus rhamnosus GIM 1.325 (LR1.325) were used to ferment Shatianyu homogenate. During fermentation, the reducing sugar content, pH and acidity were dynamically monitored, the naringin and melitidin degradation rate were analyzed by HPLC, and the bitterness of Shatianyu homogenate after fermentation was also evaluated to determine the optimal fermentation conditions. Then, the fermented SFP was prepared, and its bitterness, together with total phenolic, total flavonoids, total DF (TDF), soluble DF (SDF), and insoluble DF (IDF) content were determined, respectively. Furthermore, the oxygen radical absorbance capacity (ORAC), ferric reducing antioxidant power (FRAP), ABTS free radical scavenging ability, together with water retention (WRC), water swelling (WSC), and oil adsorption capacity (OAC) of fermented SFP were determined, respectively.【Result】 SFP exhibited a more bitter taste, which was mainly caused by the co-flavor of naringin and melitidin, the main flavonoid components in SFP. During the first 12 h-fermentation, LP1.1516, LC1.411, LA1.731 and LR1.325 could use the reducing sugar in the homogenate and produce acids, while during 12-24 h-fermentation, only LR1.325 could still use the reducing sugar and produce acids. Shatianyu homogenate fermented by LR1.325 for 12 h showed the highest total degradation rates of naringin and melitidin (41.1%), also showed the highest sensory bitter removal rates (66.0%). Compared with unfermented SFP, the taste of SFP prepared by 12 h-LR1.325 fermentation changed from bitterer to between little bitter and slightly bitter, with a sensory bitter removal rate of 60.8%. Furthermore, the later showed higher total flavonoid content (increasing 19.8%), lower IDF (decreasing 9.6%), and higher SDF content (increasing 19.6%). Also, SFP prepared by 12 h-LR1.325 fermentation showed higher ORAC antioxidant activity (increasing 24.1%), higher water retention (increasing 20.8%) and water swelling capacity (increasing 32.0%).【Conclusion】 12 h-LR1.325 fermentation of Shatianyu homogenate consisting of septum and pulp generates low-bitterness SFP enriched in flavonoids and DF, which could be used in the development of related functional foods.

Key words: Shatianyu (Citrus grandis L. Osbeck) fruit powder, flavonoids, dietary fiber, semi-solid fermentation with lactobacillus, debitterizing, antioxidant activity, hydration property

YE JiaMin, ZHANG MingWei, LU Qi, ZHANG RuiFen, DENG Mei. Effects of Semi-Solid Fermentation with Lactobacillus on the Bitterness and Active Components of Shatianyu (Citrus grandis L. Osbeck) Fruit Powder[J].Scientia Agricultura Sinica, 2024, 57(13): 2662-2673.

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References 43

[1]	DENG M, ZHANG S, DONG L H, HUANG F, JIA X C, SU D X, CHI J W, MUHAMMAD Z, MA Q, ZHAO D, ZHANG M W, ZHANG R F. Shatianyu (Citrus grandis L. Osbeck) flavonoids and dietary fiber in combination are more effective than individually in alleviating high-fat-diet-induced hyperlipidemia in mice by altering gut microbiota. Journal of Agricultural and Food Chemistry, 2022, 70(46): 14654-14664.
[2]	DENG M, ZHANG R F, ZHANG S, LU Q, DONG L H, HUANG F, JIA X C, MA Q, CHI J W, ZHAO D, YAN S J, ZHANG M W. The flavonoid profiles in different tissue parts of Shatianyu (Citrus grandis L. Osbeck) and their in vitro bioactivity. LWT-Food Science and Technology, 2023, 180: 114712.
[3]	DENG M, YE J M, ZHANG S, ZHANG R F, LU Q, DONG L H, HUANG F, JIA X C, ZHANG M W. Composition, structural, physicochemical and functional properties of dietary fiber from different tissue parts of Shatianyu (Citrus grandis L. Osbeck). LWT, 2024, 191: 115581.
[4]	江飞凤, 胡鹏刚, 田太江, 谭晓辉, 潘雪梅, 闫锦. 柚子酒酶法脱苦工艺优化及香气成分分析. 现代食品科技, 2021, 37(8): 275-285.
	JIANG F F, HU P G, TIAN T J, TAN X H, PAN X M, YAN J. Optimization of enzymatic process for removing bitterness and analysis of aroma components in pomelo wine. Modern Food Science and Technology, 2021, 37(8): 275-285. (in Chinese)
[5]	LI L J, TAN W S, LI W J, ZHU Y B, CHENG Y S, NI H. Citrus taste modification potentials by genetic engineering. International Journal of Molecular Sciences, 2019, 20(24): 6194.
[6]	GORDON R M, WASHINGTON T L, SIMS C A, GOODRICH- SCHNEIDER R, BAKER S M, YAGIZ Y, GU L W. Performance of macroporous resins for debittering HLB-affected grapefruit juice and its impacts on furanocoumarin and consumer sensory acceptability. Food Chemistry, 2021, 352: 129367.
[7]	GONZÁLEZ-TEMIÑO Y, RUÍZ M O, ORTEGA N, RAMOS-GÓMEZ S, BUSTO M D. Immobilization of naringinase on asymmetric organic membranes: Application for debittering of grapefruit juice. Innovative Food Science & Emerging Technologies, 2021, 73: 102790.
[8]	GUAN C R, YUAN Y, ZHANG W J, DING X L, ZHANG C C, CHEN D W, LU M L, GU R X, CHEN X. Variation of bitter components of the asparagus juices during lactic acid bacteria fermentation. Bioscience, Biotechnology, and Biochemistry, 2021, 85(11): 2300-2310.
[9]	FERREIRA-LAZARTE A, PLAZA-VINUESA L, DE LAS RIVAS B, VILLAMIEL M, MUÑOZ R, MORENO F J. Production of α-rhamnosidases from Lactobacillus plantarum WCFS1 and their role in deglycosylation of dietary flavonoids naringin and rutin. International Journal of Biological Macromolecules, 2021, 193: 1093-1102.
[10]	XIAO J B. Dietary flavonoid aglycones and their glycosides: Which show better biological significance? Critical Reviews in Food Science and Nutrition, 2017, 57(9): 1874-1905. doi: 10.1080/10408398.2015.1032400 pmid: 26176651
[11]	霍擎. 玛咖提取物中苦味物质分析与品质控制[D]. 武汉: 华中科技大学, 2018.
	HUO Q. Analysis of bitter substances in maca extract and quality control[D]. Wuhan: Huazhong University of Science and Technology, 2018. (in Chinese)
[12]	卢琦, 贾栩超, 邓梅, 张瑞芬, 董丽红, 黄菲, 池建伟, 刘磊, 张名位. 不同干制方式对沙田柚果渣粉活性物质的影响. 中国农业科学, 2022, 55(14): 2825-2836. doi: 10.3864/j.issn.0578-1752.2022. 14.012.
	LU Q, JIA X C, DENG M, ZHANG R F, DONG L H, HUANG F, CHI J W, LIU L, ZHANG M W. Effects of different drying methods on bioactive components of shatianyou(Citrus grandis L. Osbeck) pomace powder. Scientia Agricultura Sinica, 2022, 55(14): 2825-2836. doi: 10.3864/j.issn.0578-1752.2022.14.012. (in Chinese)
[13]	文伟, 张名位, 刘磊, 张瑞芬, 魏振承, 张雁, 马永轩, 张晖. 乳酸菌发酵对脱脂米糠中糖和酚类物质含量的影响. 现代食品科技, 2016, 32(2): 137-141.
	WEN W, ZHANG M W, LIU L, ZHANG R F, WEI Z C, ZHANG Y, MA Y X, ZHANG H. Effects of lactic acid bacteria fermentation on the content of sugars and polyphenols of defatted rice bran. Modern Food Science and Technology, 2016, 32(2): 137-141. (in Chinese)
[14]	中华人民共和国国家卫生和计划生育委员会. GB 5009.239-2016, 食品安全国家标准食品酸度的测定. 北京: 中国标准出版社. 2016.
	National Health and Family Planning Commission of the P.R.C. GB 5009.239-2016, National Standard for Food Safety-Determination of Acidity in Food. Beijing: Standards Press of China. 2016. (in Chinese)
[15]	PAIVA F F, VANIER N L, DE JESUS BERRIOS J, PAN J, DE ALMEIDA VILLANOVA F, TAKEOKA G, ELIAS M C. Physicochemical and nutritional properties of pigmented rice subjected to different degrees of milling. Journal of Food Composition and Analysis, 2014, 35(1): 10-17.
[16]	赖婷, 刘磊, 张名位, 张瑞芬, 张雁, 魏振承, 邓媛元. 不同乳酸菌发酵对桂圆肉中酚类物质及抗氧化活性的影响. 中国农业科学, 2016, 49(10): 1979-1989. doi: 10.3864/j.issn.0578-1752.2016.10.013.
	LAI T, LIU L, ZHANG M W, ZHANG R F, ZHANG Y, WEI Z C, DENG Y Y. Effect of lactic acid bacteria fermentation on phenolic profiles and antioxidant activity of dried Longan flesh. Scientia Agricultura Sinica, 2016, 49(10): 1979-1989. doi: 10.3864/j.issn. 0578-1752.2016.10.013. (in Chinese)
[17]	王立峰. 薏米中多酚类物质对抗氧化、抗肿瘤和降血脂作用的评价研究[D]. 无锡: 江南大学, 2012.
	WANG L F. Evaluation research of antioxidant, antitumour activities and hypercholesterolemia-induced of polyphenol from adlay (Coix lachryma-jobi L.)[D]. Wuxi: Jiangnan University, 2012. (in Chinese)
[18]	DENG M, DENG Y Y, DONG L H, MA Y X, LIU L, HUANG F, WEI Z C, ZHANG Y, ZHANG M W, ZHANG R F. Effect of storage conditions on phenolic profiles and antioxidant activity of Litchi pericarp. Molecules, 2018, 23(9): 2276.
[19]	中华人民共和国国家卫生和计划生育委员会. GB 5009.88-2014, 食品安全国家标准食品中膳食纤维的测定. 北京: 中国标准出版社. 2015.
	National Health and Family Planning Commission of the P.R.C. GB 5009.88-2014, National Standard for Food Safety-Determination of Dietary Fiber in Food. Beijing: Standards Press of China. 2015. (in Chinese)
[20]	DENG M, LIN Y S, DONG L H, JIA X C, SHEN Y L, LIU L, CHI J W, HUANG F, ZHANG M W, ZHANG R F. Physicochemical and functional properties of dietary fiber from pummelo (Citrus grandis L. Osbeck) and grapefruit (Citrus paradisi Mcfad) cultivars. Food Bioscience, 2021, 40: 100890.
[21]	SU D X, TI H H, ZHANG R F, ZHANG M W, WEI Z C, DENG Y Y, GUO J X. Structural elucidation and cellular antioxidant activity evaluation of major antioxidant phenolics in lychee pulp. Food Chemistry, 2014, 158: 385-391. doi: 10.1016/j.foodchem.2014.02.134 pmid: 24731359
[22]	DENG M, JIA X C, DONG L H, LIU L, HUANG F, CHI J W, MA Q, ZHAO D, ZHANG M W, ZHANG R F. Structural elucidation of flavonoids from Shatianyu (Citrus grandis L. Osbeck) pulp and screening of key antioxidant components. Food Chemistry, 2022, 366: 130605.
[23]	张月婷. 乳杆菌发酵脱除柚子柚皮苷的研究[D]. 广州: 华南理工大学, 2015.
	ZHANG Y T. Study of the removal of naringin by Lactobacillus fermentation in pomelo[D]. Guangzhou: South China University of Technology, 2015. (in Chinese)
[24]	BARROCA M, SANTOS G, JOHANSSON B, GILLOTIN F, FELLER G, COLLINS T. Deciphering the factors defining the pH-dependence of a commercial glycoside hydrolase family enzyme. Enzyme and Microbial Technology, 2017, 96: 163-169.
[25]	LI N, HAN X W, XU S J, LI C Y, WEI X, LIU Y, ZHANG R, TANG X H, ZHOU J P, HUANG Z X. Glycoside hydrolase family 39 β-xylosidase of Sphingomonas showing salt/ethanol/trypsin tolerance, low-pH/low-temperature activity, and transxylosylation activity. Journal of Agricultural and Food Chemistry, 2018, 66(36): 9465-9472.
[26]	徐慧珏. 两株乳酸菌对沙田柚汁的脱苦研究[D]. 广州: 华南理工大学, 2014.
	XU H J. Study of debittering effect of two strains of Lactobacillus in Shatian pomelo juice[D]. Guangzhou: South China University of Technology, 2014. (in Chinese)
[27]	XU J, SHI X J, ZHANG X M, WANG Z Z, XIAO W, ZHAO L G. Immobilization of GH78 α-L-rhamnosidase from Thermotoga petrophilea with high-temperature-resistant magnetic particles Fe₃O₄- SiO₂-NH₂-Cellu-ZIF8 and its application in the production of prunin form naringin. Journal of Microbiology and Biotechnology, 2021, 31(3): 419-428.
[28]	LU M F, LIU S M, ZHAO L G, PEI J J. Screening β-glucosidase and α-rhamnosidase for biotransformation of naringin to naringenin by the one-pot enzymatic cascade. Enzyme and Microbial Technology, 2023, 167: 110239.
[29]	SHEHATA M G, AWAD T S, ASKER D, EL SOHAIMY S A, ABD EL-AZIZ N M, YOUSSEF M M. Antioxidant and antimicrobial activities and UPLC-ESI-MS/MS polyphenolic profile of sweet orange peel extracts. Current Research in Food Science, 2021, 4: 326-335. doi: 10.1016/j.crfs.2021.05.001 pmid: 34124691
[30]	SINGH B, SINGH J P, KAUR A, SINGH N. Phenolic composition, antioxidant potential and health benefits of citrus peel. Food Research International, 2020, 132: 109114.
[31]	KUMAR D, LADANIYA M S, GURJAR M, MENDKE S, KUMAR S, GHOSH D. Elucidation of flavanones, phenols and antioxidant capacity influenced by drying methods from physiologically dropped underutilized Citrus grandis fruits. Frontiers in Plant Science, 2023, 14: 1193635.
[32]	KUMAR D, LADANIYA M S, GURJAR M, KUMAR S, MENDKE S. Quantification of flavonoids, phenols and antioxidant potential from dropped Citrus reticulata blanco fruits influenced by drying techniques. Molecules, 2021, 26(14): 4159.
[33]	臧文静. 不同品种柑橘果实黄酮类化合物组分鉴定与抗氧化活性研究[D]. 杭州: 浙江大学, 2019.
	ZANG W J. Identification and determination of flavonoids from different Citrus cultivars fruit and their antioxidant activity[D]. Hangzhou: Zhejiang University, 2019. (in Chinese)
[34]	CHEN Y, PAN H L, HAO S X, PAN D M, WANG G J, YU W Q. Evaluation of phenolic composition and antioxidant properties of different varieties of Chinese citrus. Food Chemistry, 2021, 364: 130413.
[35]	VALDENEGRO M, BERNALES M, KNOX M, VINET R, CABALLERO E, AYALA-RASO A, KUČEROVÁ D, KUMAR R, VIKTOROVÁ J, RUML T, FIGUEROA C R, FUENTES L. Characterization of fruit development, antioxidant capacity, and potential vasoprotective action of peumo (Cryptocarya alba), a native fruit of Chile. Antioxidants, 2021, 10(12): 1997.
[36]	WANG Z W, TANG H R, LIU G W, GONG H X, LI Y G, CHEN Y L, YANG Y X. Compound probiotics producing cellulase could replace cellulase preparations during solid-state fermentation of millet bran. Bioresource Technology, 2023, 385: 129457.
[37]	杨立启. 益生菌发酵柑橘全果汁中抗氧化活性和挥发性物质研究[D]. 杭州: 浙江工业大学, 2019.
	YANG L Q. Changes in antioxidant activity and volatile compounds during the probiotic fermentation of Citrus juice[D]. Hangzhou: Zhejiang University of Technology, 2019. (in Chinese)
[38]	杨雪. 发酵法提高柑橘皮渣可溶性膳食纤维含量及产物性能的研究[D]. 南京: 南京农业大学, 2015.
	YANG X. Research on soluble dietary fiber content improvement of Citrus dregs prepared through fermentation method and product properties study[D]. Nanjing: Nanjing Agricultural University, 2015. (in Chinese)
[39]	YAN H T, KERR W L. Total phenolics content, anthocyanins, and dietary fiber content of apple pomace powders produced by vacuum-belt drying. Journal of the Science of Food and Agriculture, 2013, 93(6): 1499-1504. doi: 10.1002/jsfa.5925 pmid: 23080413
[40]	IJAZ N, AFZAAL M, NIAZ B, SAEED F, NOSHEEN F, ASIF KHAN M, HUSSAIN M, RAZA M A, IMRAN M, AL JBAWI E. Structural and functional investigations of wall material extracted from banana peels. International Journal of Food Properties, 2023, 26(1): 1636-1644.
[41]	JUREVIČIŪTĖ I, KERŠIENĖ M, BAŠINSKIENĖ L, LESKAUSKAITĖ D, JASUTIENĖ I. Characterization of berry pomace powders as dietary fiber-rich food ingredients with functional properties. Foods, 2022, 11(5): 716.
[42]	ZHUANG Z Q, CHEN M, NIU J H, QU N, JI B, DUAN X, LIU Z D, LIU X B, WANG Y T, ZHAO B T. The manufacturing process of kiwifruit fruit powder with high dietary fiber and its laxative effect. Molecules, 2019, 24(21): 3813.
[43]	ZHANG L L, ZHU M T, SHI T, GUO C, HUANG Y S, CHEN Y, XIE M Y. Recovery of dietary fiber and polyphenol from grape juice pomace and evaluation of their functional properties and polyphenol compositions. Food & Function, 2017, 8(1): 341-351.

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苦味等级 Bitterness grade	极苦 Very bitter	较苦 More bitter	稍苦 Slightly bitter	少许苦 Little bitter	无苦 Without bitter
奎宁 Quinine (g∙mL^-1)	1.0×10^-4	5.0×10^-5	2.5×10^-5	2.5×10^-6	5.0×10^-7
苦度值 Bitterness value	10	5.0	2.5	1.0	0.5

样品 Sample	苦度值 Bitterness value	苦味等级 Bitterness grade	脱苦率 Debitter rate (%)
未发酵沙田柚匀浆 Unfermented SFH	3.35±0.3c	稍苦—较苦 Slightly-More bitter	-
植物乳杆菌发酵沙田柚匀浆 SFH fermented by LP1.1516	1.52±0.1b	少许苦 Little bitter	54.6±4.8a
嗜酸乳杆菌发酵沙田柚匀浆 SFH fermented by LA1.731	1.43±0.1ab	少许苦 Little bitter	57.4±5.4a
干酪乳杆菌发酵沙田柚匀浆 SFH fermented by LC1.411	1.56±0.1b	少许苦 Little bitter	53.3±5.0a
鼠李糖乳杆菌发酵沙田柚匀浆 SFH fermented by LR1.325	1.14±0.1a	少许苦 Little bitter	66.0±5.6b

Effects of Semi-Solid Fermentation with Lactobacillus on the Bitterness and Active Components of Shatianyu (Citrus grandis L. Osbeck) Fruit Powder

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