Scientia Agricultura Sinica ›› 2024, Vol. 57 ›› Issue (6): 1180-1190.doi: 10.3864/j.issn.0578-1752.2024.06.012

• FOOD SCIENCE AND ENGINEERING • Previous Articles     Next Articles

Analysis of the Effect of Dielectric Barrier Discharge Cold Plasma on Phenolic Metabolism of Stored Paddy Rice Under High Temperature Stress

HOU Shuai1(), ZHANG YiJia1, ZHOU DanDan2, MA FeiYang1, WANG DaPeng1, ZHAO SiQi1, DING Chao1, LIU Qiang1()   

  1. 1 College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety/Jiangsu Key Laboratory of Quality Control and Further Processing of Cereals and Oil, Nanjing 210023
    2 College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037
  • Received:2023-10-07 Accepted:2024-01-24 Online:2024-03-16 Published:2024-03-25
  • Contact: LIU Qiang

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Abstract:

【Objective】 The aim of his study was to explore the impact of dielectric barrier discharge cold plasma (DBD-CP) treatment on the phenolic metabolism of stored rice under high-temperature stress.【Method】 Under simulated summer high- temperature conditions (35 ℃), fresh high-moisture rice (Japonica rice, 16.0% moisture content on a wet basis) was used as the raw material, the effects of DBD-CP treatment on quality parameters such as L*, a*, b*, yellowness index (YI), reactive oxygen species, and malondialdehyde (MDA) in stored rice were analyzed. Additionally, the study aims to analyze the changes in total phenols, total flavonoids, and antioxidant activities during the storage process. The investigation would involve multiple validations, including ABTS+ and DPPH radical scavenging activities, ferric ion reducing power, and the activity changes of key rate-limiting enzymes in phenylpropane metabolism. The ultimate goal was to explore the impact of DBD-CP treatment on the phenolic metabolism of stored rice. 【Result】 After DBD-CP treatment, the color stability of rice during storage significantly improved. The yellowing index of the treatment group decreased significantly by 12.6% after 60 days of storage. After 20 days of storage, the levels of superoxide anion, hydrogen peroxide, and MDA decreased by 14.8%, 41.6%, and 21.6%, respectively, and the deterioration rate of fresh high-moisture rice was significantly inhibited. Additionally, during the high-temperature stress storage period, the total phenol and total flavonoid content in the treatment group increased simultaneously (P<0.05), with maximum increases of 1.23 and 1.34 times, respectively. The antioxidant properties and iron ion reduction capacity of the corresponding samples were also improved (P<0.05). Combined with the analysis of the activity of key limiting enzymes in phenylpropane metabolism, the activities of phenylalanine ammonia-lyase (PAL), cinnamic acid-4-hydroxylase (C4H), and chalcone synthase (CHS) were significantly enhanced, with increases ranging from 1.71 to 2.28 times. Further correlation analysis confirmed the close association (P<0.05) between the content of phenolic substances in high-moisture rice under high-temperature stress and the activities of PAL, C4H, and CHS enzymes, and a significant negative correlation (P<0.05) with internal reactive oxygen species and MDA content. 【Conclusion】 Dielectric barrier discharge cold plasma treatment could promote the synthesis rate of phenolic compounds within the grains of high-moisture rice by activating phenylpropane metabolism. This treatment enhanced the tissue's antioxidant properties, alleviated membrane lipid peroxidation, and reduced the accumulation of free radicals. As a result, it delayed the quality deterioration of rice under high-temperature storage conditions.

Key words: DBD-CP, phenylpropanoid metabolism, phenolic compounds, antioxidant activity, storage

Fig. 1

The effect of DBD-CP treatment on the apparent color of rice during storage *: Significant difference (P<0.05); **: Extremely significant difference (P<0.01). The same as below"

Fig. 2

Effect of DBD-CP treatment on the content of total phenols and total flavonoids of rice during storage"

Fig. 3

Effect of DBD-CP treatment on the oxidation resistance of rice during storage"

Fig. 4

Effect of DBD-CP treatment on active oxygen content in rice grain during storage"

Fig. 5

Effect of DBD-CP treatment on MDA content in rice"

Fig. 6

Effect of DBD-CP treatment on the activities of key enzymes involved in phenylpropanoid metabolism in rice"

Fig. 7

Correlation analysis between phenylpropanoid metabolism and phenolic compounds in rice treated with DBD-CP"

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