Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (17): 3726-3736.doi: 10.3864/j.issn.0578-1752.2021.17.014

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Effects of Nitrogen Application Rate on Nitrogen Use Efficiency, Yield and Quality of Late Sowing Rapeseed

LI XiaoYong1(),GU ChiMing1,LIU Kang1,LIAO Xing1,HUANG Wei2,YANG ZhiYuan3,QIN Lu1()   

  1. 1Oil Crops Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetics Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062
    2Huanggang Academy of Agricultural Sciences, Huanggang 438000, Hubei
    3Yingcheng Agricultural Technology Extension Center, Yingcheng 432400, Hubei
  • Received:2020-09-27 Accepted:2020-11-11 Online:2021-09-01 Published:2021-09-09
  • Contact: Lu QIN E-mail:dashuai_17@163.com;qinlu-123@126.com

Abstract:

【Objective】 Under the multi-cropping system in the Yangtze River Basin, the rapeseed sowing date was delayed, resulting in low yield and poor quality. In this study, in order to explore the effect of different nitrogen rates on the yield and seed quality of late sowing rapeseed, the related indexes of late sowing rapeseed, including yield and its components, agronomic characters, nutrient accumulation, grain quality, and so on, were measured under different nitrogen application levels in order to find out the best nitrogen rate of late sowing rapeseed. 【Method】 Field experiments were carried out in Yingcheng and Huanggang, Hubei province. A rapeseed variety“Zhongyouza19”was used as the material, and two sowing dates (S1,10th October; S2, 10th November) and 4 nitrogen rates (N0, 0; N1, 120 kg·hm-2; N2, 240 kg·hm-2; N3, 360 kg·hm-2) were set in this study. 【Result】 (1) Late sowing of rapeseed significantly reduced the yield. With an increase in nitrogen rate, the pods per plant, seeds per pod and 1000-seed weight had a significant increase under different sowing dates. Compared with N0, the yield under N1, N2 and N3 was increased by 31.9%, 68.6% and 79.8% on average under normal sowing date, respectively, and increased by 36.0%, 82.0% and 87.3% on average under late sowing date, respectively. (2) With the sowing date delaying, the root crown diameter, plant height and branch height were decreased significantly, and the above-ground biomass were increased, whereas the root biomass and the root-shoot ratio were decreased. With an increase in nitrogen rate, the root crown diameter, plant height, branch height, the root and shoot biomass were increased, but the root-shoot ratio was decreased, in addition, the lodging angle were increased by 162.7%, 254.7% and 374.7% on average under normal sowing date, respectively, and increased by 105.5%, 208.7% and 303.1% on average under late sowing date, respectively. Lodging was more severe under higher nitrogen rate. (3) With the sowing date delaying, the nitrogen content and nitrogen uptake in the root were decreased. The nitrogen content in the seed was increased, but the nitrogen uptake and oil content in the seed were decreased. Then, the nitrogen content in the stem and the pod shell did not change significantly, but the nitrogen uptake increased significantly. With an increase in nitrogen rate, the nitrogen content and uptake in each part were increased and the oil yield also showed an increasing trend, but the oil yield (1 830.5 kg·hm-2 and 1 534.5 kg·hm-2 (Yingcheng), 1 535.1 kg·hm-2 and 1 220.0 kg·hm-2(Huanggang)) and the seed nitrogen use efficiency (34.88% and 31.14% (Yingcheng), 27.95% and 25.48% (Huanggang)) reached the maximum at the nitrogen rate level of 240 kg·hm-2under different sowing dates. There was a significant positive correlation between seed nitrogen use efficiency and grain yield and oil yield, but there was no correlation with lodging angle. The grain yield and oil yield could be improved by increasing seed nitrogen use efficiency without aggravating lodging. 【Conclusion】 Increasing nitrogen rate could improve the yield of late sowing rapeseed, but the nitrogen rate should be controlled at about 240 kg·hm-2 to further control lodging and obtain maximum oil yield.

Key words: rapeseed (Brassica napus L.), sowing date, nitrogen rate, yield, oil yield

Fig. 1

Average temperature at different test sites"

Table 1

Effects of sowing dates and nitrogen rates on yield and yield components of rapeseed"

播期
Sowing date
施氮量
Nitrogen rate
应城 Yingcheng 黄冈 Huanggang
单株角果数
Effective pods per plant
每角果粒数
Seeds per pod
千粒重1000-seed weight (g) 单株产量
Yield per plant (g)
实际产量
Yield (kg·hm-2)
单株角果数
Effective pods per plant
每角果粒数
Seeds per pod
千粒重1000-seed weight (g) 单株产量
Yield per plant (g)
实际产量
Yield (kg·hm-2)
S1 N0 60.7c 17.6c 4.29c 4.57e 2058.6e 67.3b 17.2c 3.91d 4.53e 2039.4e
N1 72.7b 20.4b 4.45c 6.59c 2964.2c 69.5b 19.1b 4.09c 5.42d 2438.1d
N2 78.4a 21.2b 4.92ab 8.17a 3674.3a 82.8a 19.5ab 4.45b 7.16b 3223.1b
N3 78.9a 21.2b 5.07a 8.48a 3818.7a 85.0a 19.0b 4.87a 7.88a 3546.2a
S2 N0 39.8e 21.7b 4.43c 3.82f 1719.6f 43.5e 18.9b 4.13c 3.39f 1526.1f
N1 48.8d 23.8a 4.72b 5.48d 2465.8d 49.7d 20.2ab 4.31b 4.33e 1947.0e
N2 59.2c 24.2a 4.98a 7.14bc 3215.0b 61.7c 20.4ab 4.78a 6.00c 2699.2c
N3 63.0c 23.0a 5.00a 7.26b 3265.9b 62.8c 21.0a 4.75a 6.26c 2815.9c
方差分析Variance analysis
S ** ** NS ** ** ** ** ** ** **
N ** ** ** ** ** ** ** ** ** **
S×N NS NS NS NS NS NS NS ** NS NS

Fig. 2

Seed quality at different sowing dates and nitrogen rates of rapeseed"

Table 2

Effects of sowing dates and nitrogen rates on agronomic traits of rapeseed at maturity"

播期
Sowing date
施氮量
Nitrogen rate
应城 Yingcheng 黄冈 Huanggang
根颈粗
Root crown diameter (mm)
株高
Plant height
(cm)
分枝起点高度
Branch height (cm)
根颈粗
Root crown diameter (mm)
株高
Plant height
(cm)
分枝起点高度
Branch height (cm)
S1 N0 9.41d 153.40bc 102.50c 8.03f 142.77de 96.11c
N1 11.95b 179.00a 119.46b 11.52c 165.73ab 111.43b
N2 12.09b 180.85a 121.29b 12.33b 176.80a 118.44a
N3 13.10a 183.00a 125.45a 13.24a 178.25a 120.47a
S2 N0 5.68f 144.50c 83.93f 6.53g 136.20e 75.25f
N1 8.50e 154.00bc 87.53f 9.51e 149.00cde 81.50e
N2 9.45d 156.67bc 92.86e 10.17d 154.62bcd 87.37d
N3 11.47c 160.90b 97.33d 11.21c 159.35bc 92.35c
方差分析Variance analysis
S ** ** ** ** ** **
N ** ** ** ** ** **
S×N ** NS ** * NS **

Fig. 3

Aboveground biomass, root biomass and root-shoot ratio at different sowing dates and nitrogen rates of rapeseed"

Fig. 4

Lodging angle at different sowing dates and nitrogen rates of rapeseed"

Table 3

Effects of sowing dates and nitrogen rates on nitrogen content and uptake"

试验点
Trial site
播期
Sowing date
施氮量
Nitrogen rate
根 Root 茎 Stem 角果壳 Pod shell 籽粒 Grain
氮含量
N content
(%)
氮积累量
N uptake(kg·hm-2
氮含量
N content
(%)
氮积累量
N uptake(kg·hm-2
氮含量
N content
(%)
氮积累量
N uptake(kg·hm-2
氮含量
N content (%)
氮积累量
N uptake(kg·hm-2
应城 Yingcheng S1 N0 0.60cd 3.63cd 0.39d 4.28g 0.59c 7.47f 3.21c 65.96e
N1 0.65bcd 4.25c 0.58cd 26.30e 0.65bc 15.65de 3.45c 102.27d
N2 0.82bc 6.76b 0.77bc 40.15d 0.75bc 21.21c 4.07b 149.67bc
N3 1.37a 11.46a 1.19a 66.16b 1.21a 34.52b 4.61a 176.18a
S2 N0 0.50d 2.83d 0.47cd 10.93f 0.52c 11.22ef 3.36c 57.80e
N1 0.59cd 3.50cd 0.60cd 35.43d 0.66bc 16.84cd 3.58c 88.23d
N2 0.66bcd 4.24c 0.94ab 57.73c 0.84b 31.78b 4.12b 132.55c
N3 0.84b 6.55b 1.22a 85.92a 1.18a 47.00a 4.94a 161.35ab
黄冈 Huanggang S1 N0 0.67de 3.73de 0.52d 8.91g 0.73c 7.79f 3.31e 67.48ef
N1 0.87c 6.27c 0.74c 30.81e 0.94b 23.94d 3.62d 88.22d
N2 1.10b 9.47b 1.06b 53.83c 1.00b 27.94d 4.17c 134.56b
N3 1.51a 13.45a 1.23a 71.04b 1.44a 46.02b 4.72b 167.26a
S2 N0 0.57e 2.93e 0.58d 16.04f 0.79c 14.94e 3.46de 52.87f
N1 0.74d 4.78d 0.76c 36.53d 0.96b 26.16d 3.68d 71.68de
N2 1.05b 8.36b 1.07b 68.87b 1.01b 33.63c 4.22c 114.06c
N3 1.08b 9.09b 1.27a 92.54a 1.46a 57.07a 5.04a 142.00b
方差分析 Variance analysis
S ** ** NS ** NS ** ** **
N ** ** ** ** ** ** ** **
S×N ** ** NS ** NS ** NS NS

Fig. 5

Effect of sowing dates and nitrogen rates on nitrogen use efficiency"

Table 4

Correlation coefficients between nitrogen uptake and other yield and quality traits"

实际产量 Yield 含油率 Oil content 产油量 Oil yield 倒伏角度 Lodging angle
根氮积累量 Root nitrogen uptake 0.731** -0.717** 0.568* 0.586*
茎氮积累量 Stem nitrogen uptake 0.688** -0.932** 0.461 0.974**
角果壳氮积累量 Pod shell nitrogen uptake 0.587* -0.961** 0.330 0.941**
籽粒氮积累量 Seed nitrogen uptake 0.956** -0.702** 0.832** 0.785**
籽粒氮利用率 Seed nitrogen use efficiency 0.860** 0.123 0.887** 0.168
氮生理效率 Nitrogen physiological efficiency -0.543* 0.923** -0.312 -0.932**
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