Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (5): 856-864.doi: 10.3864/j.issn.0578-1752.2014.05.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity Analysis of 328 Maize Varieties (Hybridized Combinations) Using SSR Markers

 WANG  Feng-Ge, TIAN  Hong-Li, ZHAO  Jiu-Ran, WANG  Lu, YI  Hong-Mei, SONG  Wei, GAO  Yu-Qian, YANG  Guo-Hang   

  1. Maize Research Center, Beijing Academy of Agriculture & Forestry Sciences, Beijing 100097
  • Received:2013-09-04 Online:2014-03-01 Published:2013-12-02

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Abstract: 【Objective】 The objective of this study is to analyze the genetic diversity of 328 maize varieties from breeding years, planting regions, and to research the genetic differentiation characteristics of all the varieties from every appropriate planting region. This study will provide a theoretical basis for the maize regional trial, validation management and breeding strategic adjustment. 【Method】 Forty core SSR primers covering the entire maize genome were used to scan 328 samples by high-throughput 10-plex capillary electrophoresis platform. The polymorphism of 40 SSR loci and genetic diversity of 328 samples were evaluated via the software Power-Marker ver.3.25, and the Principal Coordinate Analysis (PCoA) of these samples from every regional trial groups was executed using statistical analysis software MVSP ver.3.13. 【Result】 The detected alleles of 40 SSR primers ranged from 3 to 16, with an average of 8.10, the polymorphism information index (PIC) ranged from 0.18 to 0.85, with an average of 0.63. There were no significant genetic diversity changes among varieties from different years, and all the PIC values were about 0.60. The total allele and gene-type number of 8 regional trial groups ranged from 170 to 262 and from 200 to 511. The varieties from Jing-Jin-Tang and Xi-Nan displayed the minimum and maximum values. The varieties from Jing-Jin-Tang group displayed the lowest PIC value of 0.51, and the remaining groups were near 0.60, and the average heterozygosity was about 0.60. Principal coordinate analysis (PCoA) of varieties from eight regional trial groups showed that the genetic distribution of varieties from Xian-Shi (maize for table use) and Ji-Zao-Shu (very early maturity maize) groups deviated from the common maize region, displayed an obvious germplasm specificity. As the common maize varieties were used as control samples at early stage, the genetic distribution of Qing-Chu (silage corn) extended to common maize. The genetic distribution of varieties from Jing-Jin-Tang, Xi-Nan and Dong-Bei-Zao groups was relatively concentrated. The genetic distribution of varieties from Ji-Zao-Shu, Jing-Jin-Tang and Xi-Nan groups was almost no overlap. There is an obvious genetic variation of varieties from Dong-Hua-Bei and Huang-Huai-Hai groups, but also there are some overlaps, and the reasons might be the same control variety and the same breeding resource. 【Conclusion】 Genetic diversity and genetic differentiation of 328 maize varieties were analyzed using 40 SSR markers. The results showed that there were no significant genetic diversity changes among varieties in different years, and there were also no significant changes among different groups except that from Jing-Jin-Tang. Principal coordinate analysis showed that the setting of regional trial groups and control varieties has played a guiding role in breeding.

Key words: maize , SSR marker , regional trial , genetic diversity

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