Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (5): 856-864.doi: 10.3864/j.issn.0578-1752.2014.05.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity Analysis of 328 Maize Varieties (Hybridized Combinations) Using SSR Markers

 WANG  Feng-Ge, TIAN  Hong-Li, ZHAO  Jiu-Ran, WANG  Lu, YI  Hong-Mei, SONG  Wei, GAO  Yu-Qian, YANG  Guo-Hang   

  1. Maize Research Center, Beijing Academy of Agriculture & Forestry Sciences, Beijing 100097
  • Received:2013-09-04 Online:2014-03-01 Published:2013-12-02

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Abstract: 【Objective】 The objective of this study is to analyze the genetic diversity of 328 maize varieties from breeding years, planting regions, and to research the genetic differentiation characteristics of all the varieties from every appropriate planting region. This study will provide a theoretical basis for the maize regional trial, validation management and breeding strategic adjustment. 【Method】 Forty core SSR primers covering the entire maize genome were used to scan 328 samples by high-throughput 10-plex capillary electrophoresis platform. The polymorphism of 40 SSR loci and genetic diversity of 328 samples were evaluated via the software Power-Marker ver.3.25, and the Principal Coordinate Analysis (PCoA) of these samples from every regional trial groups was executed using statistical analysis software MVSP ver.3.13. 【Result】 The detected alleles of 40 SSR primers ranged from 3 to 16, with an average of 8.10, the polymorphism information index (PIC) ranged from 0.18 to 0.85, with an average of 0.63. There were no significant genetic diversity changes among varieties from different years, and all the PIC values were about 0.60. The total allele and gene-type number of 8 regional trial groups ranged from 170 to 262 and from 200 to 511. The varieties from Jing-Jin-Tang and Xi-Nan displayed the minimum and maximum values. The varieties from Jing-Jin-Tang group displayed the lowest PIC value of 0.51, and the remaining groups were near 0.60, and the average heterozygosity was about 0.60. Principal coordinate analysis (PCoA) of varieties from eight regional trial groups showed that the genetic distribution of varieties from Xian-Shi (maize for table use) and Ji-Zao-Shu (very early maturity maize) groups deviated from the common maize region, displayed an obvious germplasm specificity. As the common maize varieties were used as control samples at early stage, the genetic distribution of Qing-Chu (silage corn) extended to common maize. The genetic distribution of varieties from Jing-Jin-Tang, Xi-Nan and Dong-Bei-Zao groups was relatively concentrated. The genetic distribution of varieties from Ji-Zao-Shu, Jing-Jin-Tang and Xi-Nan groups was almost no overlap. There is an obvious genetic variation of varieties from Dong-Hua-Bei and Huang-Huai-Hai groups, but also there are some overlaps, and the reasons might be the same control variety and the same breeding resource. 【Conclusion】 Genetic diversity and genetic differentiation of 328 maize varieties were analyzed using 40 SSR markers. The results showed that there were no significant genetic diversity changes among varieties in different years, and there were also no significant changes among different groups except that from Jing-Jin-Tang. Principal coordinate analysis showed that the setting of regional trial groups and control varieties has played a guiding role in breeding.

Key words: maize , SSR marker , regional trial , genetic diversity

[1]Hartings H, Berardo N, Mazzinelli G F, Valoti P, Verderio A, Motto M. Assessment of genetic diversity and relationships among maize (Zea mays L.) Italian landrace by morphological traits and AFLP profiling. Theoretical and Applied Genetics, 2008, 117: 831-842.

[2]Lu H, Bernardo R. Molecular marker diversity among current and historical maize inbreds. Theoretical and Applied Genetics, 2001, 103: 613-617.

[3]Yu Y, Wang R, Shi Y, Song Y, Wang T, Li Y. Genetic diversity and structure of the core collection for maize inbred lines in China. Maydica, 2007, 52: 181-194.

[4]Barcaccia G, Lucchin M, Parrini P. Characterization of a flint maize (Zea mays var. indurate） Italian landrace: II. Genetic diversity and relatedness assessed by SSR and Inter-SSR molecular markers. Genetic Resource and Crop Evolution, 2003, 50: 253-271.

[5]Smith J S C, Chin E C L, Shu H, Smith O S, Wall S J, Senior M L, Mitchell S E, Kresovich S, Ziegle J. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): Comparisons with data from RFLPS and pedigree. Theoretical and Applied Genetics, 1997, 95: 163-173.

[6]Garcia A A F, Benchimol L L, Barbosa A M M, Geraldi I O, Souza Jr. C L, Souza A P. Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines. Genetics and Molecular Biology, 2004, 27: 579-588.

[7]齐永文, 张冬玲, 张洪亮, 王美兴, 孙俊立, 廖登群, 魏兴华, 裘宗恩, 汤圣祥, 曹永生, 王象坤, 李自超. 中国水稻选育品种遗传多样性及其近50年变化趋势. 科学通报, 2006, 51(6): 693-699.

Qi Y W, Zhang D L, Zhang H L, Wang M X, Sun J L, Liao D Q, Wei X H, Qiu Z E, Tang S X, Cao Y S, Wang X K, Li Z C. Genetic diversity of rice cultivars (Oryza Sativa L.) in China and the temporal trends in recent fifty years. Chinese Science Bulletin, 2006, 51(6): 681-688. (in Chinese)

[8]郝晨阳, 王兰芬, 张学勇, 游光霞, 董玉琛, 贾继增, 刘旭, 尚勋武, 刘三才, 曹永生. 我国育成小麦品种的遗传多样性演变. 中国科学C辑, 2005, 35(5): 408-415.

Hao C Y, Wang L F, Zhang X Y, You G X, Dong Y S, Jia J Z, Liu X, Shang X W, Liu S C, Cao Y S. Genetic diversity in Chinese modern wheat varieties revealed by microsatellite markers. Science in China: Series C Life Sciences, 2006, 49(3): 218-226.(in Chinese)

[9]郝晨阳, 董玉琛, 王兰芬, 游光霞, 张洪娜, 盖红梅, 贾继增, 张学勇. 我国普通小麦核心种质的构建及遗传多样性分析. 科学通报, 2008, 53(8): 908-915.

Hao C Y, Dong Y C, Wang L F, You G X, Zhang H N, Gai H M, Jia J Z, Zhang X Y. Genetic diversity and construction of core collection in Chinese wheat genetic resources. Chinese Science Bulletin, 2008, 53(10): 1518-1526. (in Chinese)

[10]文自翔, 赵团结, 丁艳来, 盖钧镒. 中国栽培及野生大豆的遗传多样性、地理分化和演化关系研究. 科学通报, 2009, 54(21): 3301-3310.

Wen Z X, Zhao T J, Ding Y L, Gai J Y. Genetic diversity, geographic differentiation and evolutionary relationship among ecotypes of Glycine max and G. soja in China. Chinese Science Bulletin, 2009, 54(23): 4393-4403. (in Chinese)

[11]Gethi J G, Labate J A, Lamkey K R, Smith M E, Kresovich S. SSR variation in important U.S. maize inbred lines. Crop Science, 2002, 42: 951-957.

[12]腾文涛, 曹靖生, 陈彦惠, 刘向辉, 景希强, 张发军, 李建生. 十年来中国玉米杂种优势群及其模式变化的分析. 中国农业科学, 2004, 37(12): 1804-1811.

Teng W T, Cao J S, Chen Y H, Liu X H, Jing X Q, Zhang F J, Li J S. Analysis of maize heterotic groups and patterns during past ecade in China. Scientia Agricultura Sinica, 2004, 37(12): 1804-1811. (in Chinese)

[13]Legesse B W, Myburg A A, Pixley K V, Botha A M. Genetic diversity of African maize inbred lines revealed by SSR markers. Hereditas, 2007, 144: 10-17.

[14]Xie C X, Ren W B, Sun G L, Peng J H. Inferring genome ancestry and estimating molecuar relatedness among 187 Chinese maize inbred lines. Journal of Genetics and Genomics, 2007, 34(8): 738-748.

[15]Lu Y L, Yan J B, Guimarães C T, Taba S, Hao Z F, Gao S B, Chen S J, Li J S, Zhang S H, Vivek B S, Magorokosho C, Mugo S, Makumbi D, Parentoni S N, Shah T, Rong T, Crouch J H, Xu Yunbi. Molecular characterization of global maize breeding germplasm based on genome-wide single nucleotide polymorphims. Theoretical and Applied Genetics, 2009, 120: 93-115.

[16]Yao Q L, Yang K C, Pan G T, Rong T Z. Genetic diversity of maize (Zea mays L.) landraces from southwest China based on SSR data. Journal of Genetics and Genomics, 2007, 34: 851-860.

[17]Liu Z Z, Guo R H, Zhao J R, Cai Y L, Wang F G, Cao M J, Wang R H, Shi Y S, Song Y C, Wang T Y, Li Y. Population structure and genetci diversity of maize landraces from the southwest mazie region of China. Maydica, 2009, 54: 63-76.

[18]曾三省. 中国玉米杂交种的种质基础. 中国农业科学, 1990, 23(4): 1-9.

Zeng S X. The maize germplasm base of hybreds in China. Scientia Agricultura Sinica, 1990, 23(4):1-9. (in Chinese)

[19]李俊芳, 孙世贤, 王守才. 国家玉米主产区预试品种的SSR分析: II. 预试品种的遗传多样性. 玉米科学, 2007, 15(1): 16-20.

Li J F, Sun S X, Wang S C. Analysis of maize variety in national main production area using SSR technique: II Genetic diversity of maize variety. Journal of Maize Sciences, 2007, 15(1): 16-20. (in Chinese)

[20]陈发波, 杨克诚, 荣廷昭, 潘光堂. 西南及四川区试玉米组合遗传多样性分析. 作物学报. 2007, 33(6): 991-998.

Chen F B, Yang K C, Rong T Z, Pan G T. Analysis of genetic diversity of maize hybrids in the regional tests of sichuan and southwest China. Acta Agronomica Scinica, 2007, 33(6): 991-998. (in Chinese)

[21]赵静, 孙娟, 张仁和, 薛吉全. 基于SSR技术分析陕西省玉米主栽品种的遗传多样性. 西北农业学报, 2008, 17(3): 124-128.

Zhao J, Sun J, Zhang R H, Xue J Q. Genetic diversity of main maize cultivars in Shaanxi province analysed by SSR markers. Acta Agriculturae Boreali-occidentalis Sinica, 2008, 17(3): 124-128. (in Chinese)

[22]Reif J C, Hamrit S, Heckenberger M, Schipprack W, Maurer H P, Bohn M, Melchinger A E. Trends in genetic diversity among European maize cultivars and their parental components during the past 50 years. Theoretical and Applied Genetics, 2005, 111: 838-845.

[23]CIMMYT. Laboratory Protocols: CIMMYT Applied Molecular Genetics Laboratory. Third Edition. Mexico, D.F.: CIMMYT. 2005.

[24]Wang F G, Tian H L, Zhao J R, Yi H M, Wang L, Song W. Development and characterization of a core set of SSR markers for fingerprinting analysis of Chinese maize varieties. Maydica, 2011, 56: 7-17.

[25]王凤格, 赵久然, 孙世贤, 支巨振, 易红梅, 宋伟, 田红丽, 杨国航. 玉米品种DNA指纹鉴定技术-SSR标记的研究与应用. 北京: 中国农业科学技术出版社, 2011: 68-70.

Wang F G, Zhao J R, Sun S X, Zhi J Z, Yi H M, Song W, Tian H L, Yang G H. DNA Fingerprinting Technology of Maize Varieties- Research and Application of SSR Markers. Beijing: China Agriculture Science Technology Press, 2011: 68-70. (in Chinese)

[26]Liu K, Muse S V. PowerMarker: An integrated analysis environment for genetic marker analysis. Bioinformatics, 2005, 21: 2128-2129.

[27]Yeh F C, Yang R C, Boyle T. Popgene version 1.31 Microsoft Window-based Freeware for population genetic analysis. Centre for International Forestry Research, University of Alberta and Tim Boyle, Edmonton, Alta, Canada. 1999.

[28]Nei M. Genetic distance between populations. The American Naturalist, 1972, 106: 283-292.

[29]Buckler E S, Stevens N M. Maize Origins, Domestication, and selection//Motley T J, Zerega N, Cross H. Darwin’s Harvest. Columbia University Press, New York, 2005: 67-90.
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