Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (2): 324-333.doi: 10.3864/j.issn.0578-1752.2014.02.012

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Screening, Identification of Lignin-Degradating Bacillus MN-8 and Its Characteristics in Degradation of Maize Straw Lignin

 LI  Hong-Ya, LI  Shu-Na, WANG  Shu-Xiang, WANG  Quan, ZHU  Bao-Cheng   

  1. College of Life Sciences, Agricultural University of Hebei, Baoding 071000, Hebei
  • Received:2013-03-01 Online:2014-01-15 Published:2013-03-01

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Abstract: 【Objective】The objective of this study is to obtain the Bacillus with the high lignin-degradation ability and investigate its characteristics in degradation of maize straw lignin, and to provide a basis for microbiological degradation of lignin. 【Method】Bacillus with high ability for degrading lignin was isolated and screened from cattle manure by the decolorization of aniline blue in plate cultivation and the lignin degradation enzyme activity was determined. Among of the obtained Bacillus, the strain with higher ability for degrading lignin was identified through morphology, physiological and biochemical tests, 16S rDNA and gyrB sequence. Then the straw heap fermentation experiment was performed with the inoculation of the bacterial strain obtained above. The changes of lignin peroxidase (LiP), manganese peroxidase (MnP) and the content of lignin in maize straw in the fermentaiton process were monitored to study the lignin-degradation activity of the strain. Its mechanism for degrading lignin was clarified by the GC/MS analysis results of lignin-degradation products. 【Result】 A strain of spore bacterium MN-8 with high ability of lignin-degradation was isolated and screened. Based on the morphology, physiological and biochemical experiments and 16S rDNA sequence analysis, strain MN-8 was identified as Bacillus. The result of 16S rDNA sequence analysis showed that MN-8 was more than 99% similar with Bacillus licheniformis and B. amyloliquefaciens. But the phylogenetic tree based on gyrB sequences showed that strain MN-8 shared 99% identity with B. amyloliquefaciens. Combined with 16S rDNA and gyrB sequence analysis, the strain MN-8 was confirmed as B. amyloliquefaciens. After 16 d incubation in the process of straw heap fermentation, lignin degradation rate was up to 24%. In the two stages of 6-8 d and 10-12 d in the stacking fermentation, the maximum activities of MnP and LiP were appeared. Lignin was degradated apparently in the same stage. And the analysis results of degradation products by GC/MS showed that strain MN-8 could degradate lignin efficiently into some low-molecular-weight matter, such as aromatic compounds 4-hydroxy-3,5-dimethoxy-acetophenone and short chain fatty acids, etc. 【Conclusion】B. amyloliquefaciens MN-8 with high lignin-degradation ability was obtained. It could degradate straw lignin into small molecular substances such as aromatic compounds by the rupture of β-O-4 bond connected between lignin monomer. And its degradation capacity depends on the LiP and MnP.

Key words: lignin-degradation , bacillus , screening , identification , degradation characteristics

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