Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (10): 1891-1902.doi: 10.3864/j.issn.0578-1752.2022.10.002


Screening of Sweetpotato Varieties Tolerant to Low Nitrogen at Seedling Stage and Evaluation of Different Nitrogen Efficiencies

FAN WenJing1(),LIU Ming2,ZHAO Peng2,ZHANG QiangQiang2,WU DeXiang1,GUO PengYu1,ZHU XiaoYa2,JIN Rong2,ZHANG AiJun2,TANG ZhongHou2()   

  1. 1College of Agronomy, Anhui Agricultural University, Hefei 230036
    2Xuhuai Area of Jiangsu Province Xuzhou Institute of Agricultural Sciences of Xuhuai District of Jiangsu Province/Xuzhou Sweetpotato Research Center of Jiangsu Province/Key Laboratory of Sweetpotato Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Xuzhou 221131, Jiangsu
  • Received:2021-11-16 Accepted:2021-12-31 Online:2022-05-16 Published:2022-06-02
  • Contact: ZhongHou TANG;


【Objective】The objective of this article was to establish a low-N tolerance evaluation system for sweetpotato varieties, to screen low-N-tolerant genotypes and evaluate different N efficiency categories and to provide a theoretical basis for studying the low-N-tolerant physiological mechanism of sweetpotato varieties and mining N-efficient genes.【Method】Under the treatment of low N stress (0 mmol·L-1) and normal N application (14 mmol·L-1) of hydroponic experiment. Selected 126 sweetpotato varieties from different sweetpotato areas at home and abroad, we collected eleven indicators, including the shoot biomass, shoot biomass increase, root biomass increase, plant biomass increase ratio, root-to-shoot ratio, vine length, root length, leaf number, CCI, nitrogen accumulation, nitrogen physiological utilization efficiency, and calculated the low-N tolerance index of all indicators. The study carried out principal component analysis by using the comprehensive membership function method, regression analysis and cluster analysis to comprehensively evaluate the low-N-tolerant sweetpotato varieties and N efficiency types.【Result】1) Under low N level, the average of the shoot biomass, shoot biomass increase, root biomass increase, plant biomass increase ratio, vine length, root length, leaf number, CCI, and N accumulation of 126 tested sweetpotato varieties was lower than that under normal N level, while the average of root-to-shoot ratio and nitrogen physiological utilization efficiency were higher than that under normal N level; 2) The variation coefficient of shoot biomass, shoot biomass increase, root biomass increase, plant biomass increase ratio, root-to-shoot ratio, vine length, root length, leaf number, nitrogen accumulation, nitrogen physiological utilization efficiency at low-N stress was higher than that at normal N level, and the amplification of them were ranked as shoot biomass increase>plant biomass increase ratio>root biomass increase>leaf number>shoot biomass>nitrogen physiological utilization efficiency>nitrogen accumulation>root length> root-to-shoot ratio>Vine length; 3) The principal component analysis was carried out on the low-N-tolerant index of eleven indicators, extracted three principal components, the cumulative variance contribution rate of which was 72.67%, and calculated the comprehensive evaluation Y-value; 4) The correlation between the low-N-tolerant index of shoot biomass, shoot biomass increase, root biomass increase, plant biomass increase ratio, leaf number, vine length, root length, root-to-shoot ratio, nitrogen accumulation, nitrogen physiological utilization efficiency and the Y-value were highly significant(P<0.01), among them, the correlation of shoot biomass increase, root biomass increase, plant biomass increase ratio, nitrogen accumulation and shoot biomass was higher, with the correlation coefficients of 0.85, 0.86, 0.81, 0.79 and 0.73, respectively; 5) The regression equation of the Y-value screened eight indicators to evaluate low-N tolerance of sweetpotato varieties, and the cluster analysis on eight indicators showed that the sweetpotato genotypes were classified into three types, low-N-tolerant, intermediate, and low-N-sensitive. The agronomic traits and N efficiency traits of three sweetpotato categories were analyzed by variance analysis.【Conclusion】Shoot biomass, shoot biomass increase, root biomass increase, root length, vine length, leaf number, nitrogen accumulation and nitrogen physiological utilization efficiency were selected as the low-N tolerance evaluation indicators of sweetpotato; The study screened 7 low-N-tolerant sweetpotato varieties: 13104-2/Zishu1, Yibinhongxinshu, Zhezishu 2, Yuzi 3, Yuzi 6, Luozi 1 and Yuzixiang 10; The results of variance analysis showed that the low-N-tolerant varieties performed better than the intermediate and the low-N sensitive varieties, and there are significant difference in five traits: shoot biomass, shoot biomass increase, root biomass increase, vine length and nitrogen accumulation.

Key words: sweetpotato, low-N tolerance, screening indexes, comprehensive evaluation, N efficiency categories

Table 1

Variation analysis of various traits at different levels of nitrogen supply"

正常氮处理CK 低氮处理N0
CV (%)
CV (%)
地上干重 Shoot biomass (g) 0.83—4.44 2.27 33.68 0.50—4.38 1.87 48.27
地上干物质增加量 Shoot biomass increase (g) 0.26—3.39 1.45 45.40 0.01—2.75 0.79 70.52
地下干物质增加量 Root biomass increase (g) 0.10—1.57 0.66 46.31 0.12—1.61 0.60 52.57
总干物质增加率 Plant biomass increase ratio (%) 45.61—632.24 267.50 43.12 9.04—497.51 145.17 60.47
根冠比 Root shoot ratio 0.12—0.63 0.29 32.71 0.08—0.87 0.34 41.78
蔓长 Vine length (cm) 19.93—42.73 29.75 16.80 18.27—54.60 29.10 21.68
根长 Root length (cm) 11.10—68.33 41.37 29.68 7.80—91.60 37.33 39.19
叶数 Leaf number 4—11 6.53 19.96 4—17 6.23 33.45
CCI值 Chlorophyll content index 11.77—53.67 25.30 27.23 5.13—21.83 13.03 21.91
氮积累量 N accumulation (g/plant) 0.087—0.50 0.24 36.78 0.03—0.28 0.10 49.38
Nitrogen physiological utilization efficiency (%)
4.00—11.53 8.52 13.66 2.34—22.32 13.24 28.86

Fig. 1

PC dispersion points of low-nitrogen tolerance index of each indictor"

Table 2

Weighting coefficients, eigenvalues, variance contribution rate and cumulative variance contribution rate of the 3 principal components of each trait index"

主成分 Principal components
地上干重Shoot biomass 0.3703 -0.3193 -0.2293
地上干物质增加量Shoot biomass increase 0.3983 -0.0773 -0.2555
地下干物质增加量Root biomass increase 0.3795 0.0727 -0.0871
总干物质增加率Plant biomass increase ratio 0.3327 0.3361 -0.1239
根冠比Root shoot ratio 0.1134 0.5903 0.1480
蔓长Vine length 0.3174 -0.2588 0.3445
根长Root length 0.2092 0.0521 0.7267
叶数Leaf number 0.2753 0.0314 0.2342
CCI值Chlorophyll content index 0.7529 -0.1891 0.2893
氮积累量 N accumulation 0.3878 -0.2664 -0.1674
氮素生理利用效率 Nitrogen physiological utilization efficiency 0.2464 0.4977 -0.1568
特征值Eigenvalues 5.2190 1.7060 1.0680
方差贡献率 Variance contribution rate (%) 47.4460 15.5130 9.7060
累计方差贡献率 Cumulative variance contribution rate (%) 47.4460 62.9590 72.6650

Table 3

Correlations of the comprehensive value Y for low-N tolerance with index in sweetpotato"

Correlation coefficient
P value
地上干重Shoot biomass 0.730** 0.000
地上干物质增加量Shoot biomass increase 0.848** 0.000
地下干物质增加量Root biomass increase 0.858** 0.000
总干物质增加率Plant biomass increase ratio 0.812** 0.000
根冠比Root shoot ratio 0.408** 0.000
蔓长Vine length 0.680** 0.000
根长Root length 0.549** 0.000
叶数Leaf number 0.645** 0.000
CCI值Chlorophyll content index 0.150 0.093
氮积累量 N accumulation 0.788** 0.000
Nitrogen physiological utilization efficiency
0.655** 0.000

Fig. 2

Cluster analysis diagram of 8 related indexes of low nitrogen stress tolerance index"

Table 4

Evaluation of traits of low-nitrogen-tolerant, intermediate, and low nitrogen susceptible sweetpotatoes"

Shoot biomass increase
Root biomass increase
N accumulation
Nitrogen physiological utilization efficiency
Low nitrogen tolerance
2.33±0.37a 2.81±0.84a 3.67±1.49a 1.45±0.76a 1.57±0.24a 1.42±0.38a 1.39±0.23a 2.10±0.39a
1.627±0.45b 1.23±0.45b 2.33±0.64b 1.39±0.47a 1.26±0.34b 1.09±0.46b 0.92±0.24b 1.77±0.55ab
Low nitrogen
0.76±0.37c 0.51±0.35c 0.86±0.42c 0.91±0.25b 0.94±0.20c 0.90±0.35b 0.40±0.19c 1.52±0.47b
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