Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (9): 1714-1722.doi: 10.3864/j.issn.0578-1752.2012.09.006

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Screening and Identification of Sorghum Cultivars for Salinity Tolerance During Germination

 SUN  Lu, ZHOU  Yu-Fei, WANG  Che, XIAO  Mu-Ji, TAO  Ye, XU  Wen-Juan, HUANG  Rui-Dong   

  1. 1.沈阳农业大学农学院，沈阳 110866
    2.沈阳农业大学生物科学技术学院，沈阳 110866
  • Received:2011-12-27 Online:2012-05-01 Published:2012-03-19

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Abstract: 【Objective】The objectives of the current study were to explore the response of different sorghum cultivars to salt stress, screen sorghum cultivars suitable for growing in saline soils, and provide a foundation for management of salt stress.【Method】 Thirty seeds of each of 42 sorghum cultivars were treated with 150 mmol•L-1NaCl or distilled water and incubated in Petri dishes for 10 days at humidity of 60%, light/dark of 12h/12h, illumination of 34 μmol•m-2•s-1 and 28℃/25℃ of day/night. Germination and growth were assessed by counting the number of radicals and coleoptiles and leaves that had appeared on a daily basis from the fourth day and measuring their lengths and dry weights on the tenth day using destructive sampling. The cultivars were classified on the basis of salinity tolerance by means of principal component analysis and cluster analysis considering the range of data on germination and growth traits.【Result】The correlation analysis on the relative values of most germination and growth parameters showed positive relationships among several variables. The cultivars were ranked based on the principal components analysis and five groups on salinity tolerance were sorted for the 42 sorghum cultivars according to the cluster analysis.【Conclusion】Principal component analysis results showed that radical length, coleoptiles and leaf weight and germination rate were the most significant factors and are recommended as the main indexes to identify salinity tolerance of sorghum at germination. The analysis showed five of the 42 cultivars were highly salt tolerant, e.g. Liaoza 15, fourteen cultivars were salt tolerant, e.g. Shenshi 104, twelve cultivars were medium salt sensitive, e.g. Aoza 1, eight cultivars were salt sensitive, e.g Tieza 17, and three were highly salt sensitive, e.g. Longza 10. As this research only relates to ranking sensitivity during germination, further work is needed to determine the mechanism of tolerance and its persistence through the plant life cycle.

Key words: sorghum, NaCl stress, salinity tolerance, principal components analysis, cluster analysis

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