Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (9): 1723-1730.doi: 10.3864/j.issn.0578-1752.2012.09.007

• PLANT PROTECTION • Previous Articles     Next Articles

Seasonal Changes in the Cold Hardiness of Chrysopa pallens (Rambur) Prepupa

 YU  Ling-Yuan, SHI  Ai-Ju, ZHENG  Fang-Qiang, LU  Hong, ZHANG  Fan, XU  Yong-Yu   

  1. 1.山东农业大学植物保护学院，山东泰安 271018
    2.北京市农林科学院植物保护环境保护研究所，北京 100097
  • Received:2011-09-06 Online:2012-05-01 Published:2011-11-04

PDF

638

Cited

3

Abstract: 【Objective】The objective of this study is to understand the seasonal changes of cold hardiness of Chrysopa pallens (Rambur) prepupa in the natural conditions. 【Method】The wet weight, supercooling point (SCP) and freezing point (FP), the contents of water, fat, sugar and protein in the prepupa body were determined in different times. 【Result】The SCP and FP of     Ch. pallens prepupa were the lowest in January 2010 (mid-winter period), -17.53 and -7.14℃, respectively, and the highest in July, -8.21 and -3.65℃, respectively. The SCP and FP of prepupa in September and October of 2009 (pre-winter period) were not significantly lower than that in July of 2010 (growth period) and lower than that in April and May of 2010 (post-winter periods). The change patterns of the contents of the water, sugar and protein in the prepupa were similar as that of SCP with decreasing first and increasing later from September 2009 to July 2010, but the changes of content of fat were on the contrary, with higher fat content during winter and lower in other seasons. The highest fat content in prepupa was 46.81% in November 2009 (pre-winter period) and the lowest was 31.07% in July 2010 (growth period).【Conclusion】The cold hardiness of Ch. pallens prepupa varied obviously with seasonal changes, and pre- and mid-winter prepupa had higher cold tolerance than those in the summer and post-winter. The results suggest that the cold hardiness of the prepupa may be related to the contents of water and some biochemical substances in the prepupa body in the natural conditions．

Key words: Chrysopa pallens (Rambur), prepupa, cold hardiness, seasonal changes

[1]赵敬钊. 大草蛉生物学特性研究. 植物保护学报, 1988, 15(2): 123-127.

Zhao J Z. Studies on the bionomics of Chrysopa septempunctata Wesmoer. Acta Phytophylacica Sinica, 1988, 15(2): 123-127. (in Chinese)

[2]牟吉元, 王念慈, 范永贵. 四种草蛉生活史和习性的研究. 植物保护学报, 1980, 7(1): 1-8.

Mu J Y, Wang N C, Fan Y G. Studies on the life histories and bionomics of four species of green lacewings. Acta Phytophylacica Sinica, 1980, 7(1): 1-8. (in Chinese)

[3]景晓红, 康  乐. 光照与飞蝗卵耐寒性的关系. 动物学研究, 2003, 24(3): 196-199.

Jing X H, Kang L. Egg cold hardiness of the migratory locust Locusta migratoria L. under different photoperiod conditions. Zoological Research, 2003, 24(3): 196-199. (in Chinese)

[4]Lee R E. Insect cold hardiness: to freeze or not to freeze. Bioscience, 1989, 39(5): 308-313.

[5]Baust J G, Rojas R R. Review-insect cold hardiness: Facts and fancy. Journal of Insect Physiology, 1985, 31(10): 755-759.

[6]Bale J S. Insect cold hardiness: Freezing and supercooling-an ecophysiological perspective. Journal of Insect Physiology, 1987, 33(12): 899-908.

[7]Danks H V. Insect adaptations to cold and changing environments. The Canadian Entomology, 2006, 138(1): 1-23.

[8]Koveos D S. Rapid cold hardening in the olive fruit fly Bactrocera oleae under laboratory and field conditions. Entomologia Experientalis et Applicata, 2001, 101(3): 257-263.

[9]Pedersen S A, Kristiansen E, Hansen B H, Andersen R A, Zachariassen K E. Cold hardiness in relation to trace metal stress in the freeze-avoiding beetle Tenebrio molitor. Journal of Insect Physiology, 2006, 52(8): 846-853.

[10]马延龙, 候  凤, 马  纪. 荒漠昆虫光滑鳖甲的耐寒性季节变化及其生理机制. 昆虫学报, 2009, 52(4): 372-379.

Ma Y L, Hou F, Ma J. Seasonal changes in cold tolerance of desert beetle Anatolica polita borealis (Coleoptera: Tenebrionidae) and their physiological mechanisms. Acta Entomologica Sinica, 2009, 52(4): 372-379. (in Chinese)

[11]张飞萍, 钟景辉, 江宝福, 粱光红, 苏  军, 任媛媛. 松突圆蚧种群耐寒性的季节变化. 生态学报, 2009, 29(11): 5813-5822.

Zhang F P, Zhong J H, Jiang B F, Liang G H, Su J, Ren Y Y. Seasonal variation in cold tolerance of the population of pine armored scale Hemiberlesia pitysophila Takagi (Homoptera：Diaspididae). Acta Ecologica Sinica, 2009, 29(11): 5813-5822. (in Chinese)

[12]赵  静, 于令媛, 李  敏, 郑方强, 张  帆, 许永玉. 异色瓢虫成虫耐寒能力的季节性变化. 昆虫学报, 2008, 51(12): 1271-1278.

Zhao J, Yu L Y, Li M, Zheng F Q, Zhang F, Xu Y Y. Seasonal variation in cold tolerance of the multicolored ladybeetle, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) adults. Acta Entomologica Sinica, 2008, 51(12): 1271-1278. (in Chinese)

[13]郭海波, 许永玉, 鞠  珍, 李明贵. 中华通草蛉成虫抗寒能力季节性变化. 生态学报, 2006, 26(10): 3238-3244.

Guo H B, Xu Y Y, Ju Z, Li M G. Seasonal changes of cold hardiness of the green lacewing, Chrysoperla sinica (Tjeder) (Neuroptera: Chrysopidae). Acta Ecologica Sinica, 2006, 26(10): 3238-3244. (in Chinese)

[14]强承魁, 杜予州, 于玲雅, 崔亚东, 陆明星, 郑福山. 水稻二化螟越冬幼虫耐寒性物质的动态变化. 应用生态学报, 2008, 19(3): 599-605.

Qiang C K, Du Y Z, Yu L Y, Cui Y D, Lu M X, Zheng F S. Dynamic changes of cold-resistant substances of overwintering Chilo suppressalis (Walker) larvae. Chinese Journal of Applied Ecology, 2008, 19(3): 599-605. (in Chinese)

[15]弓惠芬, 陈  霈, 王  瑞, 连梅力, 夏志红, 阎  毅. 光周期和温度对亚洲玉米螟滞育形成的影响. 昆虫学报, 1984, 27(3): 280-285.

Gong H F, Chen P, Wang R, Lian M L, Xia Z H, Yan Y. The influences of photoperiod and temperature on the diapause of the Asian corn borer Ostrinia furnacallis (Guenee). Acta Entomologica Sinica, 1984, 27(3): 280-285. (in Chinese)

[16]景晓红, 康  乐. 昆虫耐寒性的测定与评价方法. 昆虫知识, 2004, 40(1): 7-10.

Jing X H, Kang L. Overview and evaluation of research methodology for insect cold hardiness. Entomological Knowledge, 2004, 40(1): 7-10. (in Chinese)

[17]蒋智林, 文礼章, 李有志, 李正跃. 甘薯天蛾过冷却点变化与生化成分的关系. 昆虫知识, 2009, 46(2): 294-297.

Jiang Z L, Wen L Z, Li Y Z, Li Z Y. Relationship between the supercooling point and the biochemical componens of Agrius convolvuli. Chinese Bulletin of Entomology, 2009, 46(2): 294-297. (in Chinese)

[18]Wolfe J, Bryant G, Koster K L. What is ‘unfreezable water’, how unfreezable is it and how much is there? CryoLetters, 2002, 23(3): 157-166.

[19]Block W, Zettel J. Activity and dormancy in relation to body water and cold tolerance in a winter-active springtail (Collembola). European Journal of Entomology, 2003, 100: 305-312.

[20]梁中贵, 张卫光, 刘学辉, 孟庆英, 孙绪艮. 松阿扁叶蜂越冬幼虫体内抗寒物质分析. 昆虫知识, 2005, 42(6): 695-699.

Liang Z G, Zhang W G, Liu X H, Meng Q Y, Sun X G. Cold hardiness analysis of over wintering larava of Acantholyda posticalis. Chinese Bulletin of Entomology, 2005, 42(6): 695-699. (in Chinese)

[21]韩瑞东, 孙绪艮, 许永玉, 张卫光. 赤松毛虫越冬幼虫生化物质变化与抗寒性的关系. 生态学报, 2005, 25(6): 1352-1356.

Han R D, Sun X G, Xu Y Y, Zhang W G. The biochemical mechanism of cold-hardiness in overwintering larva of Dendrolimus spectabilis Butler (Lepidoptera: Lasiocampidae). Acta Ecologica Sinica, 2005, 25(6): 1352-1356. (in Chinese)

[22]Zachariassen K E. Is the strategy for cold hardiness in insects determined by their water balance? A study on two closely related families of beetles. Cerambycidae and Chrysomelidae. Journal of Comparative Physiology, 2008, 178(8): 977-984.

[23]Bodine J H. Factors influencing the water content and the rate of metabolism of certain Orthoptera. Journal Experimental Zoology, 1921, 32(1): 137-164.

[24]Bodine J H. Hibernation in Orthoptera. I. Physiological changes during hibernation in certain Orthoptera. Journal of Experimental Zoology, 1923, 37(5): 457-476.

[25]冯玉香, 何维勋. 降低储粮害虫抗寒力的初步研究. 中国农业气象, 1994, 15(5): 1-4.

Feng Y X, He W X. Preliminary studies on decrease the cold-hardiness of stored grain insects. Chinese Journal of Agrometeorology, 1994, 15(5): 1-4. (in Chinese)

[26]Horton D R, Lewis T M, Neven L G. Reduced cold-hardiness of pear psylla caused by exposure to external water and surfactants. The Canadian Entomologist, 1996, 128(5): 825-830.

[27]王荫长, 陈长琨，韩召军. 昆虫生物化学. 北京: 中国农业出版社, 2000: 57-60.

Wang Y C, Chen C K, Han Z J. Insect Biochemistry. Beijing: China Agriculture Press, 2000: 57-60. (in Chinese)

[28]Zachariassen K E. Physiology of cold tolerance in insects. Physiological Reviews, 1985, 65(4): 799-832.

[29]Graham L A, Liou Y C, Walker V K, Davies P L. Hyperactive antifreeze protein from beetles. Nature, 1997, 388: 727-728.
[1] LIU Zhi-ying, LI Xi-liang, LI Feng, TAO Ya, LIU Lei, WANG Zong-li, SUN Qi-zhong. Response of Alfalfa Root Traits to Fall Dormancy and Its Effect on Winter Hardiness [J]. Scientia Agricultura Sinica, 2015, 48(9): 1689-1701.
[2] LI Na, ZHOU Xiao-rong, PANG Bao-ping. Relationship Between Supercooling Capacity and Low Molecular Sugars and Polyols, and Amino Acids in Eggs of Oedaleus asiaticus [J]. Scientia Agricultura Sinica, 2014, 47(24): 4830-4839.
[3] GAO Zhen, DI Heng, ZHANG Ke-Kun, DANG Yuan, DU Yuan-Peng. A Study on Cold Hardiness of Seven Wine Grape Canes by LT-I Analysis [J]. Scientia Agricultura Sinica, 2013, 46(5): 1014-1024.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd