Abstract: 【Objective】Shatian pummelo (Citrus grandis [L.] Osbeck cv. Shatian) is a peculiar variety in China and it is difficult to regenerate embryogenic callus. Other varieties were used to cross with it in order to gain the callus for further research on cell engineering. 【Method】Shatian pummelo was used as the female parent and crossed with the somatic hybrid NS (Nova Tangelo + Succari Sweet orange, [C. reticulata Blanco × C. paradisi Macf.] cv. Nova + C. sinensis [L.] Osbeck cv. Succari). About 90 days after pollination, the embryos obtained from crosses were cultured on the solid media of MT + ME (malt extraction, 500 mg·L-1) and MT + GA3 (1 mg·L-1). 【Result】The embryogenic callus was induced from the embryoids and plantlets' hypocotyls and can be subcultured. Flow cytometry and SSR analysis verified that the callus was from the triploid hybrid. The callus showed strong capability of embryogenesis and was able to produce a large number of embryoids on MT+ Lactose (50 g·L-1）medium after subcultured for two years.【Conclusion】It is comparatively easy to gain embryogenic callus when Shatian pummelo is crossed with somatic hybrid. The callus is valuable for the conservation and utilization of Shatian pummelo germplasm.

Key words: Shatian pummelo (Citrus grandis [L.] Osbeck cv. Shatian), Embryogenic callus, Flow cytometry, SSR, Genetic identification