111份紫花苜蓿种质资源萌发期抗旱性鉴定评价

doi:10.3864/j.issn.0578-1752.2025.10.003

Abstract

Abstract:

【Objective】 To identify and evaluate the drought resistance of alfalfa germplasm resources, screen out different drought-resistant alfalfa germplasm materials, and lay a foundation for further development of drought-resistant alfalfa resources creation and breeding utilization. 【Method】 In this study, A total of 111 alfalfa germplasm resources were used as experimental materials, and drought stress conditions were simulated using a 13% PEG-6000 solution. Two treatments were established: drought stress (13% PEG-6000) and a control (distilled water). Drought tolerance at germination stage were comprehensively evaluated by the comprehensive drought resistance coefficient (CDC value) and drought resistance coefficient (D value) using nine indicators, including germination potential, germination rate, germination index, vitality index, promptness index, root length, bud length, fresh weight and dry weight. Meanwhile, single drought tolerance coefficients, correlation analysis, principal component analysis and stepwise regression analysis were conducted to identify the key indicators influencing drought resistance during germination. 【Result】 Analysis of the single drought tolerance coefficients for the nine indicators showed that vitality index, fresh weight, and promptness index were key indicators for screening drought resistance at the germination stage. The single drought tolerance coefficients of the indicators had the highest correlation among germination index and promptness index, germination rate, vitality index, with coefficients of 0.9838, 0.9495 and 0.9338, respectively. Principal component analysis transformed the nine indicators into three principal components with a cumulative contribution of 87.287%. Drought resistance of alfalfa at germination stage was identified using two methods with CDC value and D value, and it was found that the evaluation results of the two methods were highly consistent; however, the D value method was more comprehensive, reliable, and accurate, as it used the weighting coefficients to reflect the degree of influence of each indicator to the overall drought resistance of the varieties. Based on the D value, the 111 alfalfa germplasm resources were clustered into five categories: ClassⅠ(strongest drought resistance, 1 accession), Class Ⅱ (strong drought resistance, 5 accessions), Class Ⅲ (moderate drought resistance, 55 accessions), Class Ⅳ (weak drought resistance, 37 accessions), and Class Ⅴ (drought-sensitive, 13 accessions). 【Conclusion】 The D value evaluation method was found to be the most reliable for assessing drought resistance in alfalfa during the germination stage; The study identified AG37 as the strongest drought resistance germplasm, along with five strong drought resistance accessions, including AG19, AG5, AG13 and other. Vitality index and promptness index were determined to be the most suitable indicators for evaluating drought resistance in alfalfa at the germination stage.

Key words: Medicago sativa L., germination stage, germplasm resources, drought resistance, comprehensive assessment

CHEN CaiJin, MA Lin, BAO MingFang, ZHANG GuoHui, JIANG QingXue, YANG TianHui, WANG Chuan, WANG XiaoChun, GAO Ting, WANG XueMin, LIU WenHui. Identification and Evaluation of Drought Resistance for 111 Germplasm Resources of Alfalfa During Germination Stage[J].Scientia Agricultura Sinica, 2025, 58(10): 1896-1907.

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指标 Index	范围 Range	均值 Mean	均方 Mean square	变异系数 CV (%)
相对发芽势RGE	0.00-2.20	0.72	0.2914	40.22
相对发芽率RGR	0.42-2.50	0.93	0.2621	28.24
相对发芽指数RGI	0.25-2.38	0.67	0.2580	38.38
相对活力指数RVI	0.13-1.57	0.43	0.1778	41.08
相对萌发指数RPI	0.13-2.40	0.65	0.2667	41.19
相对根长RRL	0.72-2.01	1.14	0.2510	22.09
相对芽长RBL	0.48-0.90	0.73	0.0751	10.28
相对鲜重RFW	0.45-1.43	0.64	0.1120	17.44
相对干重RDW	0.71-2.65	1.13	0.2533	22.51

Kaiser-Meyer-Olkin度量值 Kaiser-Meyer-Olkin measurement value	Bartlett球形度检验 Bartlett’s sphericity test
0.717	近似卡方Approximate Chi-squared value	1461.741
	自由度Degrees freedom	36
	显著性Significance	0.000

指标 Index	主成分Principal component
指标 Index	第1主成分PC1	第2主成分PC2	第3主成分PC3
相对发芽势RGE	0.857	-0.142	0.258
相对发芽率RGR	0.936	-0.058	-0.182
相对发芽指数RGI	0.984	-0.070	-0.095
相对活力指数RVI	0.962	0.203	0.077
相对萌发指数RPI	0.978	-0.108	0.011
相对根长RRL	0.659	0.042	-0.430
相对芽长RBL	0.218	-0.265	0.836
相对鲜重RFW	0.156	0.832	0.427
相对干重RDW	0.061	0.919	-0.121
特征值Eigenvalues	4.969	1.691	1.195
贡献率Contribution rate (%)	55.216	18.789	13.282
累计贡献率Cumulative contribution (%)	55.216	74.005	87.287

编号 Number	CDC值 CDC values	编号 Number	D值 D values
AG37	1.6950	AG37	0.6986
AG19	1.2631	AG19	0.4767
AG5	1.1567	AG5	0.4696
AG13	1.1415	AG13	0.4415
AG23	1.0490	AG26	0.4225
AG16	1.0418	AG111	0.4182
AG14	1.0338	AG10	0.3969
AG15	0.9978	AG8	0.3933
AG10	0.9768	AG16	0.3922
AG11	0.9758	AG88	0.3733

Identification and Evaluation of Drought Resistance for 111 Germplasm Resources of Alfalfa During Germination Stage

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