海岛棉纤维强度QTL紧密连锁InDel分子标记开发及效应评价

doi:10.3864/j.issn.0578-1752.2025.09.002

Abstract

Abstract:

【Objective】 The objective of this study is to develop InDel molecular markers for Island cotton, which is characterized by its superior fiber quality, particularly the fiber tensile strength-a key indicator of cotton fiber quality. The study aims to validate these markers using RIL (Recombinant Inbred Line) populations and resource materials, thereby providing a theoretical foundation for breeding new varieties of Island cotton with enhanced fiber quality. 【Method】 Utilizing a previously established population of 213 Pima S-7 and 5917 F_5:6 RILs, we conducted QTL (Quantitative Trait Locus) mapping to identify the locus regulating fiber strength in Island cotton, designated qFS-chr17-1. InDel markers were designed based on whole genome sequencing (WGS) data of the parental lines, followed by the identification of polymorphic markers. Preliminary validation of these markers was performed using 40 extreme family materials selected based on phenotypic data. Genotyping was carried out on both the 213 RIL population and the 213 Island cotton resource population, alongside multi-year fiber quality data to assess the markers' effectiveness. 【Result】 The genotyping of the RIL and Island cotton resource populations with the two developed InDel markers indicated a close linkage to fiber strength phenotypic data, with significant differences observed in fiber strength traits among the differentiated materials. The analysis of genotypic combinations revealed an upward trend in fiber strength across four combination types, with materials exhibiting the Hap3 (B/A) and Hap4 (B/B) genotypes demonstrating significantly greater fiber strength than those with Hap1 (A/A) and Hap2 (A/B). Furthermore, the InDel-3L2 marker showed significant correlations with fiber length, fiber uniformity, and spinning consistency index, consistent with the observed phenotypic trends. Analysis of multi-year fiber quality data from two experimental sites revealed environmental variability in fiber quality, while temperature data indicated that the developed molecular markers are minimally influenced by environmental factors. Clustering analysis of fiber quality data from 213 Island cotton resource materials, combined with molecular marker genotyping, identified eight materials exhibiting superior fiber quality. 【Conclusion】 This study successfully developed two InDel molecular markers closely linked to the fiber strength QTL (qFS-chr17-1), which maintain their effectiveness upon combination. The InDel-3L2 marker demonstrates significant correlations with fiber length, fiber uniformity, and spinning consistency index. These markers can efficiently and accurately identify high-strength fiber resources in Island cotton, contributing to the breeding of improved fiber quality. Additionally, eight materials with excellent fiber quality have been identified.

Key words: Gossypium barbadense, fiber strength, InDel markers, germplasm resources, combined molecular markers

LÜ Tao, SUN GuoQing, GUO DongCai, CHEN QuanJia, CAI YongSheng, FAN BiaoXing, QU YanYing, ZHENG Kai. Development and Effectiveness Evaluation of InDel Molecular Markers Closely Linked to Fiber Strength QTL in Gossypium barbadense[J].Scientia Agricultura Sinica, 2025, 58(9): 1684-1701.

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References 40

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引物名称 Primer name	染色体D03 Chr. D03	插入/缺失 In/Del	扩增片段 Amplicon size (bp)	引物序列 Primer sequence (5′-3′)
InDel-3L2	3274389	Del 19bp	145	F(CCGAACAAAGGGTTAAATCG)
InDel-3L2	3274389	Del 19bp	145	R(TTCGACTGCCAGACAAATAA)
InDel-3R3	3986566	In 16bp	172	F(TAAATTTCCCTCCTTTCGGC)
InDel-3R3	3986566	In 16bp	172	R(TGATTGAGTATGGGTTTTGG)

标记Marker	Hap1	Hap2	Hap3	Hap4
InDel-3L2	A	A	B	B
InDel-3R3	A	B	A	B
总计Total	100	40	31	13

类型 Type	材料 Material	纤维长度 FL (mm)	纤维强度 FS (cN·tex^-1)	马克隆值 MIC	纤维伸长率 FE (%)	纤维整齐度 FU (%)	纺纱一致性指数 SCI
Hap4 (B/B)	GB2	33.94	41.49	4.34	5.30	86.58	199.05
	GB7	31.82	42.55	4.18	5.75	87.14	213.79
	GB8	32.93	43.88	4.08	6.08	87.55	204.30
	GB21	34.45	45.82	4.07	6.10	89.73	224.61
	GB26	32.51	45.79	4.31	4.99	88.13	215.15
	GB33	35.56	42.77	4.29	6.47	88.58	201.13
	GB37	33.87	46.32	4.10	6.40	87.59	210.13
	GB85	34.30	42.58	4.04	5.34	86.15	216.90
Hap3 (B/A)	GB3	32.77	43.94	4.14	5.60	87.54	204.97
	GB5	34.74	42.57	4.24	6.41	88.20	202.22
	GB6	33.73	42.70	4.08	5.65	88.23	217.74
	GB15	34.67	43.84	4.27	5.34	87.94	208.15
	GB16	34.61	43.16	4.29	5.87	88.17	206.27
	GB18	33.99	41.99	4.18	5.81	87.73	204.03
	GB20	34.83	44.36	4.15	5.76	88.45	211.63
	GB23	32.45	42.89	4.23	5.98	88.49	210.73
	GB35	33.51	42.18	4.55	5.54	87.06	195.89
	GB38	32.66	44.28	4.43	6.01	87.78	201.15
	GB58	32.92	43.33	4.52	5.47	87.96	218.97

Development and Effectiveness Evaluation of InDel Molecular Markers Closely Linked to Fiber Strength QTL in Gossypium barbadense

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年份 Year	环境点Environment
	6月June		7月July		8月August
	库尔勒Korla	阿瓦提Awati	库尔勒Korla	阿瓦提Awati	库尔勒Korla	阿瓦提Awati
2015	14	29	3	10	7	26
2016	9	8	6	14	19	30
2017	10	17	2	15	11	25
2018	8	28	6	15	5	19
2020	22	25	6	28	12	25
2021	29	27	10	13	28	30
2022	14	19	17	16	19	28
2023	13	16	11	13	14	21

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