Scientia Agricultura Sinica ›› 2025, Vol. 58 ›› Issue (23): 4825-4836.doi: 10.3864/j.issn.0578-1752.2025.23.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity Analysis of Phenotypic Traits of 244 Forage Oat Germplasm Resources

CHEN CaiJin1,2,3(), MA Lin1(), JIANG QingXue1, LIU JinHui1, MIAO Tong1, ZHANG ZhiPeng1, MENG Xiang1, MA XiaoRan1, ZHOU XinYue1, ZHANG Jian1,2, LIU WenHui2,*(), WANG XueMin1,*()   

  1. 1 Institute of Animal Science of Chinese Academy of Agricultural Sciences, Beijing 100193
    2 Academy of Animal Science and Veterinary Medicine of Qinghai University, Xining 810016
    3 Guyuan Branch, Ningxia Academy of Agricultural and Forestry Sciences, Guyuan 756000, Ningxia
  • Received:2025-06-04 Accepted:2025-08-22 Online:2025-12-01 Published:2025-12-09
  • Contact: LIU WenHui, WANG XueMin

Abstract:

【Objective】 To analyze the genetic diversity relationship of phenotypic traits in forage oat germplasm resources worldwide, conduct Cluster analysis and Comprehensive evaluation, explore excellent materials, and provide Material basis and Technical support for Germplasm creation and Breeding utilization of forage oats. 【Method】 Using 244 collected domestic and foreign forage oat germplasm as experimental materials, they were planted in Guyuan City, Ningxia from 2023 to 2024, and 11 Phenotypic traits including plant height, stem diameter, tiller number, leaf number, leaf length, leaf width, ear length, whorled layers number, number of spikelets, fresh weight, and hay weight were systematically measured. Multiple evaluation methods such as correlation analysis, principal component analysis, membership function, and hierarchical cluster analysis were comprehensively used for multi-dimensional evaluation. 【Result】 Phenotypic variant analysis showed that the coefficient of variation of the 11 traits ranged from 12.11% to 42.69%, among which tiller number, leaf length, and number of spikelets had higher coefficient of variation, which were 42.69%, 32.90%, and 32.77% respectively; The genetic diversity index ranged from 1.408 to 2.077, among which number of spikelets, stem diameter, and hay weight had higher genetic diversity index, which were 2.077, 2.075, and 2.074 respectively; Correlation analysis revealed that there were 41 pairs of significant or extremely significant relationships among the 11 traits, and the indicators with higher correlation with hay weight of forage oats were fresh weight, leaf number, leaf width, whorled layers number, plant height, and number of spikelets in sequence; The principal component analysis showed that the contribution rate of six principal components were extracted, which were 28.980%, 14.833%, 12.494%, 9.556%, 7.495%, and 6.850% in sequence, with the Cumulative contribution rate reaching 80.209%; Hierarchical cluster analysis divided the 244 germplasm resources into 5 Groups. Among them, the germplasm resources in Group Ⅱ had thick stem diameter, long ear length, and many whorled layers number, belonging to lodging-resistant or high yield materials; The germplasm in Group Ⅲ and Ⅳ had high plant height, long leaf length, and many tiller number, belonging to high yield forage materials; The germplasm in Group Ⅴ had multiple leaves, wide Leaf width, many number of spikelets, and high fresh and hay weight, belonging to high-quality forage type and high grain yield materials. The Comprehensive phenotypic evaluation value (F value) of germplasm resources was obtained through analytical methods such as membership function, and 20 accessions of Core germplasm resources with excellent comprehensive traits were screened out. 【Conclusion】 The results revealed that the 244 forage oat germplasm resources possess rich phenotypic genetic variation and diversity; Cluster analysis divided the 244 germplasm resources into 5 different groups, which can be selected and utilized according to the excellent characteristics of different germplasm resources and breeding objectives; 20 accessions of forage oat germplasm resources with excellent comprehensive traits, such as As166, As129, As163, As131 and As80 were screened out using F value.

Key words: Avena sativa L., germplasm resources, phenotypic traits, genetic diversity, principal component analysis, clustering analysis

Fig. 1

Frequency distribution of quantitative traits in 244 forage oat germplasm resources PH: Plant height; SD: Stem diameter; TN: Tiller number; LN: Leaf number; LL: Leaf length; LW: Leaf width; EL: Ear length; WLN: Whorled layers number; NS: Number of spikelets; FW: Fresh weight; HW: Hay weight. The same as below"

Table 1

Descriptive statistics of quantitative traits in 244 forage oat germplasm resources"

性状
Character
最小值
Minimum
最大值
Maximum
均值
Mean
极差
Range
标准差
SD
变异系数
CV (%)
遗传多样性指数
H'
株高PH (cm) 74.00 156.00 122.72 82.00 14.86 12.11 2.014
茎粗SD (mm) 3.80 11.64 7.81 7.84 1.49 19.02 2.075
分蘖数TN 3.00 52.00 17.98 49.00 7.67 42.69 2.008
叶片数LN 3.00 8.00 5.29 5.00 1.07 20.23 1.474
叶长LL (cm) 10.50 60.50 29.40 50.00 9.67 32.90 2.004
叶宽LW (cm) 0.80 3.60 2.21 2.80 0.50 22.67 2.039
穗长EL (cm) 15.80 48.80 31.63 33.00 5.87 18.55 2.049
轮生层数WLN 3.00 9.00 6.19 6.00 1.02 16.51 1.408
小穗数NS 21.00 167.00 92.43 146.00 30.28 32.77 2.077
鲜重FW (kg) 0.05 0.37 0.18 0.32 0.05 30.08 2.062
干重HW (kg) 0.02 0.11 0.06 0.09 0.02 32.75 2.074

Fig. 2

Correlation analysis plot for 11 quantitative traits * and ** means significant (P<0.05) and highly significant (P<0.01) correlations between traits, respectively"

Table 2

KMO and Bartlett's test of sphericity table"

Kaiser-Meyer-Olkin度量值
Kaiser-Meyer-Olkin measurement value
Bartlett的球形度检验
Bartlett’s sphericity test
0.687 近似卡方 Approximate Chi-squared value 自由度 df Sig.
658.636 55 0.000

Table 3

Principal component analysis table for 11 quantitative traits"

性状
Character
主成分Principal component
PC1 PC2 PC3 PC4 PC5 PC6
株高PH 0.504 0.223 0.493 0.379 -0.081 -0.447
茎粗SD 0.531 -0.233 -0.050 -0.419 0.378 -0.466
分蘖数TN -0.256 0.420 0.394 0.329 0.628 0.224
叶片数LN 0.308 0.663 0.205 -0.254 -0.408 0.025
叶长LL 0.448 -0.506 -0.151 0.609 -0.070 -0.004
叶宽LW 0.745 -0.325 -0.098 0.083 -0.014 0.099
穗长EL 0.679 0.088 0.493 0.012 -0.069 0.034
轮生层数WLN 0.673 -0.084 0.107 -0.041 -0.068 0.485
小穗数NS 0.662 -0.095 0.064 -0.373 0.277 0.192
鲜重FW 0.448 0.428 -0.628 0.154 0.029 -0.015
干重HW 0.428 0.586 -0.497 0.132 0.149 -0.046
贡献率Contribution rate (%) 28.980 14.833 12.494 9.556 7.495 6.850

Fig. 3

Cluster analysis map of 244 forage oat germplasm resources"

Table 4

Top 20 forage oat germplasm resources"

编号
Number
F
F value
排位
Rank
所属类群
Classification group
编号
Number
F
F value
排位
Rank
所属类群
Classification group
As166 0.7619 1 As88 0.6609 11
As129 0.7504 2 As82 0.6588 12
As163 0.7338 3 As47 0.6576 13
As131 0.6931 4 As170 0.6569 14
As80 0.6910 5 As190 0.6559 15
As158 0.6760 6 As157 0.6512 16
As73 0.6682 7 As93 0.6476 17
As49 0.6669 8 As92 0.6463 18
As29 0.6654 9 As78 0.6451 19
As74 0.6636 10 As96 0.6435 20
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